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The map label for this gene is dsbA

Identifier: 21672682

GI number: 21672682

Start: 470145

End: 470774

Strand: Direct

Name: dsbA

Synonym: BUsg415

Alternate gene names: 21672682

Gene position: 470145-470774 (Clockwise)

Preceding gene: 21672674

Following gene: 21672683

Centisome position: 73.29

GC content: 17.78

Gene sequence:

>630_bases
ATGAAAAAAATATTGATTGTTTTATACAGTATATTTTTATCTTTTACTGCTTCGTCTTATGAATTTAATAATAAAAAAGA
ATATGAAATAGAAAAAAGAAATATTTCTAATGTTCCTAAAGTGATGCATTTTTTTTCTTTTTTTTGTCCGTATTGTTATG
AATTAGAAAAGATATACAATATTCAATCTTTAATTAAAAATAAAATAGATAAAAAAATAAAAATACAAACTTATCATGTT
AATTTTTTAGGAGGAGAATTTAGCAAAATACTAACAAAAATTTGGATAATAGCTCAAAAAATGAAAGTGGAAGAAAAAAT
TATGATGCCTATATTTAAAGAAATTCAAGAAAATAATACTATAAGTCATACTTCTAATATTAAAAATATATTTTTGCAAA
AAACAGGCATAAATAAAGATCAATACAATAAATTTTGGAATAGCTTTACAATTAAAATGCTAATAAAAAAAAATGATAAT
GATATAAATAAAATTAAATTAAATCATGTTCCTACTATGATAGTAAACGGAAAATATGTAATTGACTATTATAAATTAGA
AAAGATTTTTAAAACAAATTTCTCTAAAAAATATATAAAATTAATAAAATTTTTACTTTCAAAAAAGTAA

Upstream 100 bases:

>100_bases
GAAAATTTAACATTATATACTAGATAGGTGTATTCTTTCATTTTATGAATTGTTTTAAAATTTTTTATTTATAAAAAAAT
TATAGAATATGGATAGAAAA

Downstream 100 bases:

>100_bases
GTTAATACGATATAAATATCAAGATCTATATTTTCTATGGTAAAATTACTTTAAGTAGTAATAAATATTTTTAATATTTA
ATTTCAATCTTTTTTATACT

Product: thiol:disulfide interchange protein DsbA precursor

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKKILIVLYSIFLSFTASSYEFNNKKEYEIEKRNISNVPKVMHFFSFFCPYCYELEKIYNIQSLIKNKIDKKIKIQTYHV
NFLGGEFSKILTKIWIIAQKMKVEEKIMMPIFKEIQENNTISHTSNIKNIFLQKTGINKDQYNKFWNSFTIKMLIKKNDN
DINKIKLNHVPTMIVNGKYVIDYYKLEKIFKTNFSKKYIKLIKFLLSKK

Sequences:

>Translated_209_residues
MKKILIVLYSIFLSFTASSYEFNNKKEYEIEKRNISNVPKVMHFFSFFCPYCYELEKIYNIQSLIKNKIDKKIKIQTYHV
NFLGGEFSKILTKIWIIAQKMKVEEKIMMPIFKEIQENNTISHTSNIKNIFLQKTGINKDQYNKFWNSFTIKMLIKKNDN
DINKIKLNHVPTMIVNGKYVIDYYKLEKIFKTNFSKKYIKLIKFLLSKK
>Mature_209_residues
MKKILIVLYSIFLSFTASSYEFNNKKEYEIEKRNISNVPKVMHFFSFFCPYCYELEKIYNIQSLIKNKIDKKIKIQTYHV
NFLGGEFSKILTKIWIIAQKMKVEEKIMMPIFKEIQENNTISHTSNIKNIFLQKTGINKDQYNKFWNSFTIKMLIKKNDN
DINKIKLNHVPTMIVNGKYVIDYYKLEKIFKTNFSKKYIKLIKFLLSKK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbA subfamily

Homologues:

Organism=Escherichia coli, GI1790291, Length=209, Percent_Identity=35.4066985645933, Blast_Score=180, Evalue=6e-47,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): DSBA_BUCAP (Q8K9D1)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660749.1
- ProteinModelPortal:   Q8K9D1
- SMR:   Q8K9D1
- EnsemblBacteria:   EBBUCT00000000332
- GeneID:   1005746
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg415
- GeneTree:   EBGT00050000008080
- HOGENOM:   HBG680642
- OMA:   FNRIHTM
- ProtClustDB:   CLSK316169
- BioCyc:   BAPH198804:BUSG415-MONOMER
- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PIRSF:   PIRSF001488

Pfam domain/function: PF01323 DSBA; SSF52833 Thiordxn-like_fd

EC number: NA

Molecular weight: Translated: 25137; Mature: 25137

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS00194 THIOREDOXIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILIVLYSIFLSFTASSYEFNNKKEYEIEKRNISNVPKVMHFFSFFCPYCYELEKIYN
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IQSLIKNKIDKKIKIQTYHVNFLGGEFSKILTKIWIIAQKMKVEEKIMMPIFKEIQENNT
HHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ISHTSNIKNIFLQKTGINKDQYNKFWNSFTIKMLIKKNDNDINKIKLNHVPTMIVNGKYV
CCHHHHHHHHEEECCCCCHHHHHHHHHCEEEEEEEECCCCCCCEEEECCCCEEEECCEEE
IDYYKLEKIFKTNFSKKYIKLIKFLLSKK
EHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKILIVLYSIFLSFTASSYEFNNKKEYEIEKRNISNVPKVMHFFSFFCPYCYELEKIYN
CHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IQSLIKNKIDKKIKIQTYHVNFLGGEFSKILTKIWIIAQKMKVEEKIMMPIFKEIQENNT
HHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ISHTSNIKNIFLQKTGINKDQYNKFWNSFTIKMLIKKNDNDINKIKLNHVPTMIVNGKYV
CCHHHHHHHHEEECCCCCHHHHHHHHHCEEEEEEEECCCCCCCEEEECCCCEEEECCEEE
IDYYKLEKIFKTNFSKKYIKLIKFLLSKK
EHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438