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The map label for this gene is pol

Identifier: 21672683

GI number: 21672683

Start: 471064

End: 471927

Strand: Direct

Name: pol

Synonym: BUsg416

Alternate gene names: 21672683

Gene position: 471064-471927 (Clockwise)

Preceding gene: 21672682

Following gene: 21672685

Centisome position: 73.44

GC content: 23.84

Gene sequence:

>864_bases
ATGCCGTACACAAAAAAAAAACCGATTATCATAGTAGATGGAAGTTTATATTTATATCGTTCTTATTTTACATTTCAAAA
CTTTAACAGTAATGAAGAAAATCCGTCTGGAGCTATATATGGAATGTTAAAAACGATACAAAATATTTTAAACAAAAATT
ATAATTCAAAAAAAATTATTATTATTTTTGATTCTTCTAAAAAAAATTTTAGAAATACAATATTTAAAGAATATAAAAGT
AATAGATCAGCAATGCCTAACAAACTCTACGTACAAATCCAACCTCTTTTTAAAATACTTGAGGAAATTGGAATTAAGAC
ATTAAGTATTTTAGGAATAGAAGCCGACGATATTATTGGAAGTTTAGCATATAAACTAGAACAAAAAGGAGAACAAGTAT
TAATTGTAAGTCATGATAAAGATATGATACAACTTATAACTGATAATATTAATGTATTGAACATCAGTAAAAACAGTATT
CTTACTCCAGAGAAAATACAAGAGAAATATGGCATTTATCCTAAAGAATTTATTGATCTTTTGGCTCTAATGGGAGACTC
CTCGGATAATATTCCAGGTGTTCCTAAAATTGGTATAAAAACTGCATTATTTCTATTAAAAAAATTTTCAAATATCAAAA
ATATTTACAATAACATTGAAAAAATACAATCTTTACCCTTTCGAAATGCTAAAAATGCTGCTATTCAATTAAAAAATTAT
AAAAAAACTGCTTTTCTTTCATATCAATTAGCCAAAATAAAATTAGATGTTCCTATTAATATTACTTCTGAAGAAATTAC
TTTAAAAAAAACATGTTTTAAAAAATTATCTAATTTGATTCGATGGTATTCATTTAACGAATAA

Upstream 100 bases:

>100_bases
TCATCTATAAAATCACTTTTACTAAAAAAAATTATTTAATAGTATACTATTTTCTTTTATTTTACTAAATTCAGTTTTAA
CTAATTAAAAGATAAAATTC

Downstream 100 bases:

>100_bases
AAAATATTCATATTCAACGATATTTTTCGTACCAAAAGTTTAATTTAGATTTTAGTTTTTCAATTCCTATTTTTTTTAAA
GATGAAAATAGTTGAATATG

Product: DNA polymerase I

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MPYTKKKPIIIVDGSLYLYRSYFTFQNFNSNEENPSGAIYGMLKTIQNILNKNYNSKKIIIIFDSSKKNFRNTIFKEYKS
NRSAMPNKLYVQIQPLFKILEEIGIKTLSILGIEADDIIGSLAYKLEQKGEQVLIVSHDKDMIQLITDNINVLNISKNSI
LTPEKIQEKYGIYPKEFIDLLALMGDSSDNIPGVPKIGIKTALFLLKKFSNIKNIYNNIEKIQSLPFRNAKNAAIQLKNY
KKTAFLSYQLAKIKLDVPINITSEEITLKKTCFKKLSNLIRWYSFNE

Sequences:

>Translated_287_residues
MPYTKKKPIIIVDGSLYLYRSYFTFQNFNSNEENPSGAIYGMLKTIQNILNKNYNSKKIIIIFDSSKKNFRNTIFKEYKS
NRSAMPNKLYVQIQPLFKILEEIGIKTLSILGIEADDIIGSLAYKLEQKGEQVLIVSHDKDMIQLITDNINVLNISKNSI
LTPEKIQEKYGIYPKEFIDLLALMGDSSDNIPGVPKIGIKTALFLLKKFSNIKNIYNNIEKIQSLPFRNAKNAAIQLKNY
KKTAFLSYQLAKIKLDVPINITSEEITLKKTCFKKLSNLIRWYSFNE
>Mature_286_residues
PYTKKKPIIIVDGSLYLYRSYFTFQNFNSNEENPSGAIYGMLKTIQNILNKNYNSKKIIIIFDSSKKNFRNTIFKEYKSN
RSAMPNKLYVQIQPLFKILEEIGIKTLSILGIEADDIIGSLAYKLEQKGEQVLIVSHDKDMIQLITDNINVLNISKNSIL
TPEKIQEKYGIYPKEFIDLLALMGDSSDNIPGVPKIGIKTALFLLKKFSNIKNIYNNIEKIQSLPFRNAKNAAIQLKNYK
KTAFLSYQLAKIKLDVPINITSEEITLKKTCFKKLSNLIRWYSFNE

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain

Homologues:

Organism=Escherichia coli, GI1790294, Length=280, Percent_Identity=43.9285714285714, Blast_Score=251, Evalue=3e-68,
Organism=Escherichia coli, GI226510970, Length=238, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): EX53_BUCAP (Q8K9D0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660750.1
- ProteinModelPortal:   Q8K9D0
- SMR:   Q8K9D0
- EnsemblBacteria:   EBBUCT00000000092
- GeneID:   1005745
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg416
- GeneTree:   EBGT00050000007705
- HOGENOM:   HBG701384
- OMA:   NESEDLP
- ProtClustDB:   PRK14976
- BioCyc:   BAPH198804:BUSG416-MONOMER
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- SMART:   SM00475
- SMART:   SM00279

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; SSF47807 5_3_exo_C

EC number: 2.7.7.7

Molecular weight: Translated: 33047; Mature: 32916

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYTKKKPIIIVDGSLYLYRSYFTFQNFNSNEENPSGAIYGMLKTIQNILNKNYNSKKII
CCCCCCCCEEEECCCEEEEEEEHEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
IIFDSSKKNFRNTIFKEYKSNRSAMPNKLYVQIQPLFKILEEIGIKTLSILGIEADDIIG
EEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCEEEEECCCHHHHHH
SLAYKLEQKGEQVLIVSHDKDMIQLITDNINVLNISKNSILTPEKIQEKYGIYPKEFIDL
HHHHHHHHCCCEEEEEECCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHH
LALMGDSSDNIPGVPKIGIKTALFLLKKFSNIKNIYNNIEKIQSLPFRNAKNAAIQLKNY
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
KKTAFLSYQLAKIKLDVPINITSEEITLKKTCFKKLSNLIRWYSFNE
CCEEEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PYTKKKPIIIVDGSLYLYRSYFTFQNFNSNEENPSGAIYGMLKTIQNILNKNYNSKKII
CCCCCCCEEEECCCEEEEEEEHEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
IIFDSSKKNFRNTIFKEYKSNRSAMPNKLYVQIQPLFKILEEIGIKTLSILGIEADDIIG
EEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCEEEEECCCHHHHHH
SLAYKLEQKGEQVLIVSHDKDMIQLITDNINVLNISKNSILTPEKIQEKYGIYPKEFIDL
HHHHHHHHCCCEEEEEECCCHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCHHHHHHH
LALMGDSSDNIPGVPKIGIKTALFLLKKFSNIKNIYNNIEKIQSLPFRNAKNAAIQLKNY
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
KKTAFLSYQLAKIKLDVPINITSEEITLKKTCFKKLSNLIRWYSFNE
CCEEEEEEEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438