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The map label for this gene is cysJ

Identifier: 21672680

GI number: 21672680

Start: 465638

End: 467446

Strand: Reverse

Name: cysJ

Synonym: BUsg413

Alternate gene names: 21672680

Gene position: 467446-465638 (Counterclockwise)

Preceding gene: 21672681

Following gene: 21672679

Centisome position: 72.87

GC content: 25.21

Gene sequence:

>1809_bases
ATGAAAAATAAAAATCCCTTTAATATTTTTTTTCCCCTTAGTTTAGATAAAATAGAAAATTTAGAAAAAATTAAAAAAAA
TTGTTCTAGTATTCAATATGCTTGGCTTTCAGGTTATTTTTGGAATTTAGCTAATCAAACTCCTTCTCGATTAATATGTG
AAAAAAATGAATTTTTTCGAAAAGATAATGAAGAGAAAATAATTACTATTATATCTGCTTCTCAAACTGGAAATGCTAGA
CAGCTTGCTAAACGTTTTAATAAATATTTAAAAAATGAAAATAAGAAAACTAATTTAATTGATGCTGCTGATTATAATTT
CAAAAAAATTAAAAATGAAAGGTTTTTAATTCTAATTATTTCTACTCAAGGAGAAGGTGAACCTCCAGAAGAAGCACTAT
CTTTTTATAAATTTATTATGTCAAAAAAAGCTCCGAGATTAGAGAATTTACACTATAGTGTATTTGGATTAGGAGATGTT
TCTTATAATTTATTTTGTCAAGCAGGAAAAGATTTTGATAAAAGATTCAGCGAATTAGGAGGAAAAAGTTTACTTCATAG
ACTAGATTCAGATATTGAATATGAATCAAATTATATACAATGGTCAGAAGAGTTGTTACTTGCTATAAACAAAATAGATG
TTTCTACTTGTTCTGTGTTCAAAAAAAATGATAAAAAAAATTTTATTGATAAGTTAAATTATACAAAGTATAAACCTGCT
GTTGCTACAGTTTTATTAAATCAAAAAATTACCGGTCGAAATTCAACTAAAGATGTTCATCATATTGAGTTAGATATAAC
GAATTCAAATATTGTTTATACACCTGGTGATGCGTTAGGTGTATGGTATCAAAATAGTAGTCAATTAATAAAACAAATAT
TAAAACTACTTTCTATAAGAATATCTGATAAAGTTAAAGTTAAAGATAAAATAATTACTATTTTTGAAGCTTTGAAAAAA
AATTTTGAATTAACTACAAATACTAAGCATATTATTCAAAAATATACTGATGTTACACAAAATAAATTTTTGAAAAAAAT
TATATCTGATAATAAAAAATTAAACAACTATGTAAAAAAAACACCTTTGTTAAAGATGATATATGATCATCCTAAAAAAC
TGTCTTCTCAACAATTAATTAGTATTTTACGACCTTTAAAACCTAGATTATATTCTATTTCTTCATCTCAATCTGAAATG
AATGATGAAGTTCATATTACAGTCGGAGTAGTGAAGAAACAAATTTCTGGTACTATACATTTAGGAGGTTCTTCTAGTTA
TTTATCACAATTTTTAAAAATTGATGATTCTGTAAAAATTTTTGTTGAAGAAAAGAGTAATTTTCGTTTACCTGAAAATA
AAGATGTACCTATTATTATGATTGGATCAGGAACTGGTATCGCTCCATTTCGTGCTTTTATACAACAACGAGATAATGAT
AAAGCTACAGGAAAAAATTGGATATTTTTTGGTAATCCTAATTTTACTGAAGATTTTTTATATCAATTAGAATGGCAAAA
ATATTTGAAAAAAAAACTTCTTACTAAAATGAGTTTAGCTTGGTCACGAGATCAAAAGGAAAAAATATATGTACAAGATA
AAATAAGAGAAAATGGAAAAGAATTATGGGAATGGGTCAATCAAGGGGCACAAATCTACGTTTGTGGTAATGCATCTAAA
ATGGCAAAAGACGTTGAAAAAGAATTGTTAGATGTTTTTAGTAAGAATGGTTCAATGGATATTGAAGAATCTAGTGAGTT
TTTAAATAACTTGAGAATAACAAGACGTTATCAAAGAGATGTATACTAA

Upstream 100 bases:

>100_bases
TGACTTCTTATTAGTAGATAAAAATTAATATTTTCTGTAAATTATACTAAATTAATAATATTAGTTTTTTAAAACTAATA
AACTTTAGGCATTTAGTGTA

Downstream 100 bases:

>100_bases
TGAAAAAAGATTATAAAAAAGTATTTTTAAAAAATACTAAAGAAGAGCGAATTAAAGAAAACAGTAATTATCTTAGAGGC
ACTATTATTGACGATTTAAA

Product: sulfite reductase (NADPH) flavoprotein alpha-component

Products: NA

Alternate protein names: SiR-FP

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MKNKNPFNIFFPLSLDKIENLEKIKKNCSSIQYAWLSGYFWNLANQTPSRLICEKNEFFRKDNEEKIITIISASQTGNAR
QLAKRFNKYLKNENKKTNLIDAADYNFKKIKNERFLILIISTQGEGEPPEEALSFYKFIMSKKAPRLENLHYSVFGLGDV
SYNLFCQAGKDFDKRFSELGGKSLLHRLDSDIEYESNYIQWSEELLLAINKIDVSTCSVFKKNDKKNFIDKLNYTKYKPA
VATVLLNQKITGRNSTKDVHHIELDITNSNIVYTPGDALGVWYQNSSQLIKQILKLLSIRISDKVKVKDKIITIFEALKK
NFELTTNTKHIIQKYTDVTQNKFLKKIISDNKKLNNYVKKTPLLKMIYDHPKKLSSQQLISILRPLKPRLYSISSSQSEM
NDEVHITVGVVKKQISGTIHLGGSSSYLSQFLKIDDSVKIFVEEKSNFRLPENKDVPIIMIGSGTGIAPFRAFIQQRDND
KATGKNWIFFGNPNFTEDFLYQLEWQKYLKKKLLTKMSLAWSRDQKEKIYVQDKIRENGKELWEWVNQGAQIYVCGNASK
MAKDVEKELLDVFSKNGSMDIEESSEFLNNLRITRRYQRDVY

Sequences:

>Translated_602_residues
MKNKNPFNIFFPLSLDKIENLEKIKKNCSSIQYAWLSGYFWNLANQTPSRLICEKNEFFRKDNEEKIITIISASQTGNAR
QLAKRFNKYLKNENKKTNLIDAADYNFKKIKNERFLILIISTQGEGEPPEEALSFYKFIMSKKAPRLENLHYSVFGLGDV
SYNLFCQAGKDFDKRFSELGGKSLLHRLDSDIEYESNYIQWSEELLLAINKIDVSTCSVFKKNDKKNFIDKLNYTKYKPA
VATVLLNQKITGRNSTKDVHHIELDITNSNIVYTPGDALGVWYQNSSQLIKQILKLLSIRISDKVKVKDKIITIFEALKK
NFELTTNTKHIIQKYTDVTQNKFLKKIISDNKKLNNYVKKTPLLKMIYDHPKKLSSQQLISILRPLKPRLYSISSSQSEM
NDEVHITVGVVKKQISGTIHLGGSSSYLSQFLKIDDSVKIFVEEKSNFRLPENKDVPIIMIGSGTGIAPFRAFIQQRDND
KATGKNWIFFGNPNFTEDFLYQLEWQKYLKKKLLTKMSLAWSRDQKEKIYVQDKIRENGKELWEWVNQGAQIYVCGNASK
MAKDVEKELLDVFSKNGSMDIEESSEFLNNLRITRRYQRDVY
>Mature_602_residues
MKNKNPFNIFFPLSLDKIENLEKIKKNCSSIQYAWLSGYFWNLANQTPSRLICEKNEFFRKDNEEKIITIISASQTGNAR
QLAKRFNKYLKNENKKTNLIDAADYNFKKIKNERFLILIISTQGEGEPPEEALSFYKFIMSKKAPRLENLHYSVFGLGDV
SYNLFCQAGKDFDKRFSELGGKSLLHRLDSDIEYESNYIQWSEELLLAINKIDVSTCSVFKKNDKKNFIDKLNYTKYKPA
VATVLLNQKITGRNSTKDVHHIELDITNSNIVYTPGDALGVWYQNSSQLIKQILKLLSIRISDKVKVKDKIITIFEALKK
NFELTTNTKHIIQKYTDVTQNKFLKKIISDNKKLNNYVKKTPLLKMIYDHPKKLSSQQLISILRPLKPRLYSISSSQSEM
NDEVHITVGVVKKQISGTIHLGGSSSYLSQFLKIDDSVKIFVEEKSNFRLPENKDVPIIMIGSGTGIAPFRAFIQQRDND
KATGKNWIFFGNPNFTEDFLYQLEWQKYLKKKLLTKMSLAWSRDQKEKIYVQDKIRENGKELWEWVNQGAQIYVCGNASK
MAKDVEKELLDVFSKNGSMDIEESSEFLNNLRITRRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Homo sapiens, GI127139033, Length=607, Percent_Identity=27.3476112026359, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI7657393, Length=596, Percent_Identity=26.1744966442953, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI221316709, Length=596, Percent_Identity=26.1744966442953, Blast_Score=212, Evalue=9e-55,
Organism=Homo sapiens, GI221316705, Length=605, Percent_Identity=25.7851239669421, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI10835173, Length=654, Percent_Identity=26.9113149847095, Blast_Score=193, Evalue=5e-49,
Organism=Homo sapiens, GI221316707, Length=524, Percent_Identity=26.5267175572519, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI24041029, Length=607, Percent_Identity=28.171334431631, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI40254422, Length=659, Percent_Identity=23.6722306525038, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI169790956, Length=427, Percent_Identity=24.824355971897, Blast_Score=133, Evalue=5e-31,
Organism=Homo sapiens, GI169790958, Length=427, Percent_Identity=24.824355971897, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1789123, Length=603, Percent_Identity=49.4195688225539, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=628, Percent_Identity=28.0254777070064, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17566446, Length=585, Percent_Identity=23.9316239316239, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531441, Length=392, Percent_Identity=22.1938775510204, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=632, Percent_Identity=28.9556962025316, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321143, Length=386, Percent_Identity=27.720207253886, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6325305, Length=631, Percent_Identity=23.2963549920761, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137192, Length=612, Percent_Identity=27.4509803921569, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24582192, Length=525, Percent_Identity=27.8095238095238, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI78706872, Length=526, Percent_Identity=29.0874524714829, Blast_Score=173, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24583543, Length=526, Percent_Identity=29.0874524714829, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI78706876, Length=526, Percent_Identity=29.0874524714829, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24660907, Length=591, Percent_Identity=24.8730964467005, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24660903, Length=591, Percent_Identity=24.8730964467005, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24647438, Length=407, Percent_Identity=22.3587223587224, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSJ_BUCAP (Q8K9D3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660747.1
- ProteinModelPortal:   Q8K9D3
- SMR:   Q8K9D3
- EnsemblBacteria:   EBBUCT00000000227
- GeneID:   1005748
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg413
- GeneTree:   EBGT00050000008144
- HOGENOM:   HBG736048
- OMA:   HVEIDLG
- ProtClustDB:   CLSK316168
- BioCyc:   BAPH198804:BUSG413-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01541
- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.20.990.10
- PIRSF:   PIRSF000207
- PRINTS:   PR00369
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01931

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.8.1.2

Molecular weight: Translated: 69930; Mature: 69930

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS51384 FAD_FR; PS50902 FLAVODOXIN_LIKE

Important sites: BINDING 492-492

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKNPFNIFFPLSLDKIENLEKIKKNCSSIQYAWLSGYFWNLANQTPSRLICEKNEFFR
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCCCCHHHEECCHHHHC
KDNEEKIITIISASQTGNARQLAKRFNKYLKNENKKTNLIDAADYNFKKIKNERFLILII
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCCEEEEEEE
STQGEGEPPEEALSFYKFIMSKKAPRLENLHYSVFGLGDVSYNLFCQAGKDFDKRFSELG
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHC
GKSLLHRLDSDIEYESNYIQWSEELLLAINKIDVSTCSVFKKNDKKNFIDKLNYTKYKPA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCCHH
VATVLLNQKITGRNSTKDVHHIELDITNSNIVYTPGDALGVWYQNSSQLIKQILKLLSIR
HHHHHHCCCCCCCCCCCCEEEEEEEEECCEEEECCCCEEEEEECCHHHHHHHHHHHHHHH
ISDKVKVKDKIITIFEALKKNFELTTNTKHIIQKYTDVTQNKFLKKIISDNKKLNNYVKK
CCCCEEHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TPLLKMIYDHPKKLSSQQLISILRPLKPRLYSISSSQSEMNDEVHITVGVVKKQISGTIH
CHHHHHHHHCHHHCCHHHHHHHHHCCCCHHEECCCCHHHCCCCEEEEEEEHHHHHCCEEE
LGGSSSYLSQFLKIDDSVKIFVEEKSNFRLPENKDVPIIMIGSGTGIAPFRAFIQQRDND
ECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
KATGKNWIFFGNPNFTEDFLYQLEWQKYLKKKLLTKMSLAWSRDQKEKIYVQDKIRENGK
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCHH
ELWEWVNQGAQIYVCGNASKMAKDVEKELLDVFSKNGSMDIEESSEFLNNLRITRRYQRD
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
VY
CC
>Mature Secondary Structure
MKNKNPFNIFFPLSLDKIENLEKIKKNCSSIQYAWLSGYFWNLANQTPSRLICEKNEFFR
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHCCCCCHHHEECCHHHHC
KDNEEKIITIISASQTGNARQLAKRFNKYLKNENKKTNLIDAADYNFKKIKNERFLILII
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHCCCCEEEEEEE
STQGEGEPPEEALSFYKFIMSKKAPRLENLHYSVFGLGDVSYNLFCQAGKDFDKRFSELG
ECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHC
GKSLLHRLDSDIEYESNYIQWSEELLLAINKIDVSTCSVFKKNDKKNFIDKLNYTKYKPA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCCHH
VATVLLNQKITGRNSTKDVHHIELDITNSNIVYTPGDALGVWYQNSSQLIKQILKLLSIR
HHHHHHCCCCCCCCCCCCEEEEEEEEECCEEEECCCCEEEEEECCHHHHHHHHHHHHHHH
ISDKVKVKDKIITIFEALKKNFELTTNTKHIIQKYTDVTQNKFLKKIISDNKKLNNYVKK
CCCCEEHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TPLLKMIYDHPKKLSSQQLISILRPLKPRLYSISSSQSEMNDEVHITVGVVKKQISGTIH
CHHHHHHHHCHHHCCHHHHHHHHHCCCCHHEECCCCHHHCCCCEEEEEEEHHHHHCCEEE
LGGSSSYLSQFLKIDDSVKIFVEEKSNFRLPENKDVPIIMIGSGTGIAPFRAFIQQRDND
ECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCC
KATGKNWIFFGNPNFTEDFLYQLEWQKYLKKKLLTKMSLAWSRDQKEKIYVQDKIRENGK
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCHH
ELWEWVNQGAQIYVCGNASKMAKDVEKELLDVFSKNGSMDIEESSEFLNNLRITRRYQRD
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
VY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438