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The map label for this gene is ygcM

Identifier: 21672675

GI number: 21672675

Start: 455621

End: 455983

Strand: Reverse

Name: ygcM

Synonym: BUsg402

Alternate gene names: 21672675

Gene position: 455983-455621 (Counterclockwise)

Preceding gene: 21672676

Following gene: 21672670

Centisome position: 71.09

GC content: 27.55

Gene sequence:

>363_bases
GTGAAAACTATTATATTTAAAGATTTTCAATTCGAGGCAGCGCATTATTTACCGTATGTTCCTAAAATGCATAAATGCAG
ACGATTACACGGTCATTCCTTTTTTGTTCGTTTAGAACTTAAAGATAAAATTAATGAAAAAAATGGATGGATAATAGATT
ATGCTGAAATTAAATTAGCGTTTCAACCTATCTATGATCAGTTAGATCATCATTTTTTAAATGATATTCCTGGTTTAGAA
AATCCAACAAGTGAAATTTTAGCTAAATGGATTTGGCATCGTTTAAAACCTAAATTGTCTATTTTAAATACTATTATAAT
TAAAGAAACATGCACATCAGGATGTATTTATCAAGGTTTCTAA

Upstream 100 bases:

>100_bases
GTTATAAAGGTGAACCAGTTAATCCATTGAATTTATTACCTAACTTAAAAACACAATAACTAAAAATCTTATTTATTTTT
GAAACTATGTTGAGAAATAT

Downstream 100 bases:

>100_bases
GATTATAAACACAACTTATCGAATTTTAAGATATTTATGTATTTGTACTGATAATCTCCAATTTTTTATTATACACGTTT
TAATACAAATATTTAATGCT

Product: putative 6-pyruvoyl tetrahydrobiopterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MKTIIFKDFQFEAAHYLPYVPKMHKCRRLHGHSFFVRLELKDKINEKNGWIIDYAEIKLAFQPIYDQLDHHFLNDIPGLE
NPTSEILAKWIWHRLKPKLSILNTIIIKETCTSGCIYQGF

Sequences:

>Translated_120_residues
MKTIIFKDFQFEAAHYLPYVPKMHKCRRLHGHSFFVRLELKDKINEKNGWIIDYAEIKLAFQPIYDQLDHHFLNDIPGLE
NPTSEILAKWIWHRLKPKLSILNTIIIKETCTSGCIYQGF
>Mature_120_residues
MKTIIFKDFQFEAAHYLPYVPKMHKCRRLHGHSFFVRLELKDKINEKNGWIIDYAEIKLAFQPIYDQLDHHFLNDIPGLE
NPTSEILAKWIWHRLKPKLSILNTIIIKETCTSGCIYQGF

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily

Homologues:

Organism=Escherichia coli, GI1789124, Length=119, Percent_Identity=64.7058823529412, Blast_Score=182, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUED_BUCAP (Q8K9D8)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660742.1
- ProteinModelPortal:   Q8K9D8
- SMR:   Q8K9D8
- EnsemblBacteria:   EBBUCT00000000011
- GeneID:   1005758
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg402
- HOGENOM:   HBG727155
- OMA:   IHETCTS
- BioCyc:   BAPH198804:BUSG402-MONOMER
- InterPro:   IPR007115
- PANTHER:   PTHR12589

Pfam domain/function: PF01242 PTPS

EC number: 4.2.3.12

Molecular weight: Translated: 14273; Mature: 14273

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: ACT_SITE 26-26 ACT_SITE 70-70 ACT_SITE 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIIFKDFQFEAAHYLPYVPKMHKCRRLHGHSFFVRLELKDKINEKNGWIIDYAEIKLA
CCEEEEECCCCCHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEHHHHEEH
FQPIYDQLDHHFLNDIPGLENPTSEILAKWIWHRLKPKLSILNTIIIKETCTSGCIYQGF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCC
>Mature Secondary Structure
MKTIIFKDFQFEAAHYLPYVPKMHKCRRLHGHSFFVRLELKDKINEKNGWIIDYAEIKLA
CCEEEEECCCCCHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEEHHHHEEH
FQPIYDQLDHHFLNDIPGLENPTSEILAKWIWHRLKPKLSILNTIIIKETCTSGCIYQGF
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438