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The map label for this gene is rpiA

Identifier: 21672670

GI number: 21672670

Start: 448789

End: 449448

Strand: Reverse

Name: rpiA

Synonym: BUsg397

Alternate gene names: 21672670

Gene position: 449448-448789 (Counterclockwise)

Preceding gene: 21672675

Following gene: 21672669

Centisome position: 70.07

GC content: 28.33

Gene sequence:

>660_bases
ATGAATTTAAATGAATTAAAGAAAAAAGCGGCATGGGCTGCGCTAGATTATATCTATCCTGGAACTGTTATAGGAGTAGG
AACAGGTAGTACTGTTTTTTATTTTATTCAAGCTTTAAGTACAGTAAAACATTTAATATCTGGAGCTGTTTCTAGTTCTA
ATTCTTCTACGGTTTTATTGAAAAAAAACGGGATAAGGGTATTAGATTTAAATACCTTTGACTTCTTAGAAATTTATGTA
GACAGTGCAGATGAAATTAATAATGACATGCAAATGATTAAAGGTGGTGGAGCAGCCTTGACAAAAGAAAAAATCATTGC
TGCGATGTCAAAAAAATTTGTTTGTATTATTGATGAATCAAAAAAAGTAAATATTTTAGGCACTTTTCCATTGCCTATTG
AAATTATTCCTATAGCATTTTCTTATATTTCAAAAGAAATATTAAAAATAGGAGGACAGCCAAAATTAAGAGAAAATATT
ATAACAGATAATGGTAATGTAATTATAGATGTTTACAATTTATTTATAAATGATCCTATATCAGTAGAAAAAAAAATAAA
TTCACTACCTGGAGTAGTTAGCGTTGGTTTATTTGCTTCGAGAGTTGCAGATGTTGTTTTAATAGGTACTCAAAAAGGAG
TTGAGATTATCAAAAATTAA

Upstream 100 bases:

>100_bases
TAAAACTACAAAATTAAATTGTTAATTTAATGACATTAAAAATTTATTATTCTTTTCTATTTTAGAATATTTATTTAAAA
TAAAATAGGTTTTTTATACT

Downstream 100 bases:

>100_bases
TTCTACTAACGTTTAAAACCGTTTAAAACCGTTTGAAACCTATCAATAATATAATTTATATAGATTATTATGTCGACTAA
ACGTTCACAAAATCGAAAAG

Product: ribose-5-phosphate isomerase A

Products: NA

Alternate protein names: Phosphoriboisomerase A; PRI

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNLNELKKKAAWAALDYIYPGTVIGVGTGSTVFYFIQALSTVKHLISGAVSSSNSSTVLLKKNGIRVLDLNTFDFLEIYV
DSADEINNDMQMIKGGGAALTKEKIIAAMSKKFVCIIDESKKVNILGTFPLPIEIIPIAFSYISKEILKIGGQPKLRENI
ITDNGNVIIDVYNLFINDPISVEKKINSLPGVVSVGLFASRVADVVLIGTQKGVEIIKN

Sequences:

>Translated_219_residues
MNLNELKKKAAWAALDYIYPGTVIGVGTGSTVFYFIQALSTVKHLISGAVSSSNSSTVLLKKNGIRVLDLNTFDFLEIYV
DSADEINNDMQMIKGGGAALTKEKIIAAMSKKFVCIIDESKKVNILGTFPLPIEIIPIAFSYISKEILKIGGQPKLRENI
ITDNGNVIIDVYNLFINDPISVEKKINSLPGVVSVGLFASRVADVVLIGTQKGVEIIKN
>Mature_219_residues
MNLNELKKKAAWAALDYIYPGTVIGVGTGSTVFYFIQALSTVKHLISGAVSSSNSSTVLLKKNGIRVLDLNTFDFLEIYV
DSADEINNDMQMIKGGGAALTKEKIIAAMSKKFVCIIDESKKVNILGTFPLPIEIIPIAFSYISKEILKIGGQPKLRENI
ITDNGNVIIDVYNLFINDPISVEKKINSLPGVVSVGLFASRVADVVLIGTQKGVEIIKN

Specific function: Nonoxidative branch of the pentose phosphate pathway. [C]

COG id: COG0120

COG function: function code G; Ribose 5-phosphate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose 5-phosphate isomerase family

Homologues:

Organism=Homo sapiens, GI94536842, Length=223, Percent_Identity=33.1838565022422, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1789280, Length=217, Percent_Identity=60.8294930875576, Blast_Score=279, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI17551758, Length=230, Percent_Identity=33.9130434782609, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324669, Length=245, Percent_Identity=28.9795918367347, Blast_Score=93, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281364072, Length=196, Percent_Identity=32.6530612244898, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: 740 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): RPIA_BUCAP (Q8K9E2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660737.1
- ProteinModelPortal:   Q8K9E2
- SMR:   Q8K9E2
- EnsemblBacteria:   EBBUCT00000000526
- GeneID:   1005518
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg397
- GeneTree:   EBGT00050000007977
- HOGENOM:   HBG515603
- OMA:   VFDLNSV
- ProtClustDB:   PRK00702
- BioCyc:   BAPH198804:BUSG397-MONOMER
- HAMAP:   MF_00170
- InterPro:   IPR004788
- InterPro:   IPR020672
- PANTHER:   PTHR11934
- TIGRFAMs:   TIGR00021

Pfam domain/function: PF06026 Rib_5-P_isom_A

EC number: =5.3.1.6

Molecular weight: Translated: 23750; Mature: 23750

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNELKKKAAWAALDYIYPGTVIGVGTGSTVFYFIQALSTVKHLISGAVSSSNSSTVLL
CCHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KKNGIRVLDLNTFDFLEIYVDSADEINNDMQMIKGGGAALTKEKIIAAMSKKFVCIIDES
EECCEEEEEECCCEEEEEEECCHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECC
KKVNILGTFPLPIEIIPIAFSYISKEILKIGGQPKLRENIITDNGNVIIDVYNLFINDPI
CEEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCCHHHCEECCCCCEEEEEEEEHCCCCC
SVEKKINSLPGVVSVGLFASRVADVVLIGTQKGVEIIKN
HHHHHHHCCCCHHHHHHHHHHHCEEEEEECCCCHHHHCC
>Mature Secondary Structure
MNLNELKKKAAWAALDYIYPGTVIGVGTGSTVFYFIQALSTVKHLISGAVSSSNSSTVLL
CCHHHHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
KKNGIRVLDLNTFDFLEIYVDSADEINNDMQMIKGGGAALTKEKIIAAMSKKFVCIIDES
EECCEEEEEECCCEEEEEEECCHHHCCCCHHHHCCCCCHHHHHHHHHHHCCCEEEEEECC
KKVNILGTFPLPIEIIPIAFSYISKEILKIGGQPKLRENIITDNGNVIIDVYNLFINDPI
CEEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCCHHHCEECCCCCEEEEEEEEHCCCCC
SVEKKINSLPGVVSVGLFASRVADVVLIGTQKGVEIIKN
HHHHHHHCCCCHHHHHHHHHHHCEEEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438