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The map label for this gene is trmD

Identifier: 21672657

GI number: 21672657

Start: 434413

End: 435201

Strand: Direct

Name: trmD

Synonym: BUsg383

Alternate gene names: 21672657

Gene position: 434413-435201 (Clockwise)

Preceding gene: 21672656

Following gene: 21672658

Centisome position: 67.72

GC content: 25.98

Gene sequence:

>789_bases
ATGGAATTGAACAAACATGATCAAACTAAAACAAAATATGATAACACTTTAATATGGTTCTGTATTATTACAATTTTTCC
GGAAATGTTCTATGCAATTACAAATTATGGAGTAACTGGAAAAGCAATTCGTAAAAATATTATAAATATTAAATTTTTTA
ATCCAAGAGATTTTACTGATAATAAATATAAATCTGTAGACGATCGTCCTTACGGAGGAGGACCGGGAATGTTAATGAGT
GCAAAACCATTATATTTAGCAATTAAAAATGCAAAAAATCTATTAAAAAGTGCAATAGTTATTTATTTATCTCCTCAAGG
AAAACGATTAAATCAAAAAAAAATTCTAGAAATAATCAAAAATAAAAAAATTATTTTTGTTTGTGGTCGATATGAAGGAA
TAGATCAAAGAATTATTGATTGTCAAGTAGATGAAGAATGGTCTATAGGAGATTATATACTGACTGGAGGAGAACTGGCA
GCCATGGTTGCAATAGATTCTATTTCCAGATTTATCCCAGGAGTAATTAAAAAAAAACAATCAGTACAAGAAGATTCATT
TTTCAATGGTCTTCTTGATTATCCGCACTATACAAGACCTAAAATAATAGAAAAAATGAAAGTACCAGAAATATTACTTT
CAGGAAATCATGATAAAATTCGTTTGTGGCGTTTAAAAAAATCTTTGGAAAAAACTTGGACAAAACGACCAGATTTATTA
AAAAATAAAATTTTAAAAAAAGAAGAAAAAATACTTTTAAATGAATTAAAAAAAATTAATAAAAGGTAA

Upstream 100 bases:

>100_bases
ATAACTTAAAAAAAAATAATTTTAAAAAAATAATGATTCCTTTTATTCATAAAAAAATAGTTAAATATATTAATGTTAAA
CATAAAATTATAACAGTTAT

Downstream 100 bases:

>100_bases
ATAAAAAAATTATTAATTTTTTATCAAAAGGAAAAAAAATGACTAATATTATTCAAGAAATTGAACAAGAGCAATTGAAA
GAAAACATACCTCATTTTCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MELNKHDQTKTKYDNTLIWFCIITIFPEMFYAITNYGVTGKAIRKNIINIKFFNPRDFTDNKYKSVDDRPYGGGPGMLMS
AKPLYLAIKNAKNLLKSAIVIYLSPQGKRLNQKKILEIIKNKKIIFVCGRYEGIDQRIIDCQVDEEWSIGDYILTGGELA
AMVAIDSISRFIPGVIKKKQSVQEDSFFNGLLDYPHYTRPKIIEKMKVPEILLSGNHDKIRLWRLKKSLEKTWTKRPDLL
KNKILKKEEKILLNELKKINKR

Sequences:

>Translated_262_residues
MELNKHDQTKTKYDNTLIWFCIITIFPEMFYAITNYGVTGKAIRKNIINIKFFNPRDFTDNKYKSVDDRPYGGGPGMLMS
AKPLYLAIKNAKNLLKSAIVIYLSPQGKRLNQKKILEIIKNKKIIFVCGRYEGIDQRIIDCQVDEEWSIGDYILTGGELA
AMVAIDSISRFIPGVIKKKQSVQEDSFFNGLLDYPHYTRPKIIEKMKVPEILLSGNHDKIRLWRLKKSLEKTWTKRPDLL
KNKILKKEEKILLNELKKINKR
>Mature_262_residues
MELNKHDQTKTKYDNTLIWFCIITIFPEMFYAITNYGVTGKAIRKNIINIKFFNPRDFTDNKYKSVDDRPYGGGPGMLMS
AKPLYLAIKNAKNLLKSAIVIYLSPQGKRLNQKKILEIIKNKKIIFVCGRYEGIDQRIIDCQVDEEWSIGDYILTGGELA
AMVAIDSISRFIPGVIKKKQSVQEDSFFNGLLDYPHYTRPKIIEKMKVPEILLSGNHDKIRLWRLKKSLEKTWTKRPDLL
KNKILKKEEKILLNELKKINKR

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=56.8464730290456, Blast_Score=309, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BUCAP (Q8K9F4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660724.1
- ProteinModelPortal:   Q8K9F4
- SMR:   Q8K9F4
- EnsemblBacteria:   EBBUCT00000000137
- GeneID:   1005774
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg383
- GeneTree:   EBGT00050000008189
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   BAPH198804:BUSG383-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 30500; Mature: 30500

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNKHDQTKTKYDNTLIWFCIITIFPEMFYAITNYGVTGKAIRKNIINIKFFNPRDFTD
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCCC
NKYKSVDDRPYGGGPGMLMSAKPLYLAIKNAKNLLKSAIVIYLSPQGKRLNQKKILEIIK
CCCCCCCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHC
NKKIIFVCGRYEGIDQRIIDCQVDEEWSIGDYILTGGELAAMVAIDSISRFIPGVIKKKQ
CCCEEEEECCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SVQEDSFFNGLLDYPHYTRPKIIEKMKVPEILLSGNHDKIRLWRLKKSLEKTWTKRPDLL
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHCCCHHHH
KNKILKKEEKILLNELKKINKR
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELNKHDQTKTKYDNTLIWFCIITIFPEMFYAITNYGVTGKAIRKNIINIKFFNPRDFTD
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCCC
NKYKSVDDRPYGGGPGMLMSAKPLYLAIKNAKNLLKSAIVIYLSPQGKRLNQKKILEIIK
CCCCCCCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHC
NKKIIFVCGRYEGIDQRIIDCQVDEEWSIGDYILTGGELAAMVAIDSISRFIPGVIKKKQ
CCCEEEEECCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SVQEDSFFNGLLDYPHYTRPKIIEKMKVPEILLSGNHDKIRLWRLKKSLEKTWTKRPDLL
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHCCCHHHH
KNKILKKEEKILLNELKKINKR
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438