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The map label for this gene is pyrB

Identifier: 21672632

GI number: 21672632

Start: 408001

End: 408936

Strand: Direct

Name: pyrB

Synonym: BUsg357

Alternate gene names: 21672632

Gene position: 408001-408936 (Clockwise)

Preceding gene: 21672631

Following gene: 21672633

Centisome position: 63.61

GC content: 27.67

Gene sequence:

>936_bases
TTGAGAAATTCTCTATATAAAAAAAATATAATTTCAATTAGTGATCTTAATCAAAATGAATTAGAGTTAGTATTAAAAAA
ATCAGCTTTTCTAAAAGTAAAAGCACAACCTAATTTATTAAAAAATAAAATTATTGCCAGTTGTTTTTTTGAAGCATCAA
CACGTACTCGTTTATCTTTCGAAACTGCTGTTCATCGTTTAGGAGCATCAATAATAGGATTTTCTGATGGATCAAATATT
TCTTTAGGAAAAAAAGGCGAAACTTTATCAGACACTATTTCAGTTATAAGTTCATATGTAGATGCAATTATTATTAGACA
TCCCCAAGAAGGATCAGCACGTTTAGCCGCACAATTTTCTAATGGAGTCCCAATATTTAACGCAGGAGACGGAGCTAATC
AACACCCTACACAAACACTTTTAGATTTATTTACCATCAAAGAAACACAAAACAAGCTTAATAATTTGAATATTGCTATG
GTAGGAGATTTAAAATATGGAAGAACAGTTCATTCATTAACACAAGCATTGGCCAAGTATAAAAATAATCAATTTTTTTT
TGTTTCACCTGATTCATTAACTATGCCAAATTATATTAATGACATGCTTTATAAAAAAGAAATTCATTGGAAAAGATATA
AAAATATAGAAGAAATAATTTCTGAAATTGATATTCTTTATATGACTCGTGTTCAAAAAGAAAGACTTGATTCGACTGAA
TATGCAAGTGCTAAATCAAAATTTGTATTACAAACTTCTACTTTAAAAAATGCACGTAATAATTTAAAAATATTACATCC
ACTACCTCGTATAGACGAAATAGATAACAATGTTGACTTCACACCTTATGCTTGGTATTTTAAACAAGCAGCAAATGGAA
TTTATGCACGTCAAGCAATTTTATCATTAGTATTAATAGAAAAACATTTTGAATAA

Upstream 100 bases:

>100_bases
TATATAAAAAAATCCTCAATAATTTTTTTTCTTATCTCTTTATATATTGATCAGCGTATCAAACGCTATCTTTTTCATTA
TAATTAAGGAAAATAAAAAA

Downstream 100 bases:

>100_bases
AAAATATATGCAAATAAATAAACTTCAAGTTGAAGCTATTAAATCTGGTAGTGTAATTGATCATATTCCAGCACATATTG
GTTTTAAATTACTTTCATTA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MRNSLYKKNIISISDLNQNELELVLKKSAFLKVKAQPNLLKNKIIASCFFEASTRTRLSFETAVHRLGASIIGFSDGSNI
SLGKKGETLSDTISVISSYVDAIIIRHPQEGSARLAAQFSNGVPIFNAGDGANQHPTQTLLDLFTIKETQNKLNNLNIAM
VGDLKYGRTVHSLTQALAKYKNNQFFFVSPDSLTMPNYINDMLYKKEIHWKRYKNIEEIISEIDILYMTRVQKERLDSTE
YASAKSKFVLQTSTLKNARNNLKILHPLPRIDEIDNNVDFTPYAWYFKQAANGIYARQAILSLVLIEKHFE

Sequences:

>Translated_311_residues
MRNSLYKKNIISISDLNQNELELVLKKSAFLKVKAQPNLLKNKIIASCFFEASTRTRLSFETAVHRLGASIIGFSDGSNI
SLGKKGETLSDTISVISSYVDAIIIRHPQEGSARLAAQFSNGVPIFNAGDGANQHPTQTLLDLFTIKETQNKLNNLNIAM
VGDLKYGRTVHSLTQALAKYKNNQFFFVSPDSLTMPNYINDMLYKKEIHWKRYKNIEEIISEIDILYMTRVQKERLDSTE
YASAKSKFVLQTSTLKNARNNLKILHPLPRIDEIDNNVDFTPYAWYFKQAANGIYARQAILSLVLIEKHFE
>Mature_311_residues
MRNSLYKKNIISISDLNQNELELVLKKSAFLKVKAQPNLLKNKIIASCFFEASTRTRLSFETAVHRLGASIIGFSDGSNI
SLGKKGETLSDTISVISSYVDAIIIRHPQEGSARLAAQFSNGVPIFNAGDGANQHPTQTLLDLFTIKETQNKLNNLNIAM
VGDLKYGRTVHSLTQALAKYKNNQFFFVSPDSLTMPNYINDMLYKKEIHWKRYKNIEEIISEIDILYMTRVQKERLDSTE
YASAKSKFVLQTSTLKNARNNLKILHPLPRIDEIDNNVDFTPYAWYFKQAANGIYARQAILSLVLIEKHFE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=40.2597402597403, Blast_Score=252, Evalue=3e-67,
Organism=Homo sapiens, GI38788445, Length=323, Percent_Identity=26.9349845201238, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI2367364, Length=305, Percent_Identity=69.1803278688525, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1786469, Length=170, Percent_Identity=34.1176470588235, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790703, Length=169, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=37.5757575757576, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=39.2282958199357, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=40.9677419354839, Blast_Score=244, Evalue=7e-65,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_BUCAP (Q8K9H9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660699.1
- ProteinModelPortal:   Q8K9H9
- SMR:   Q8K9H9
- EnsemblBacteria:   EBBUCT00000000481
- GeneID:   1005798
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg357
- GeneTree:   EBGT00050000007871
- HOGENOM:   HBG579429
- OMA:   AQYIDSH
- ProtClustDB:   PRK00856
- BioCyc:   BAPH198804:BUSG357-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35315; Mature: 35315

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSLYKKNIISISDLNQNELELVLKKSAFLKVKAQPNLLKNKIIASCFFEASTRTRLSF
CCCCCCCCCCEEEECCCCHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHH
ETAVHRLGASIIGFSDGSNISLGKKGETLSDTISVISSYVDAIIIRHPQEGSARLAAQFS
HHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHEECC
NGVPIFNAGDGANQHPTQTLLDLFTIKETQNKLNNLNIAMVGDLKYGRTVHSLTQALAKY
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
KNNQFFFVSPDSLTMPNYINDMLYKKEIHWKRYKNIEEIISEIDILYMTRVQKERLDSTE
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHH
YASAKSKFVLQTSTLKNARNNLKILHPLPRIDEIDNNVDFTPYAWYFKQAANGIYARQAI
HHCCCCCEEEEHHHHHHHHCCCEEECCCCCCHHCCCCCCCCHHHHHHHHHHCCHHHHHHH
LSLVLIEKHFE
HHHHHHHHCCC
>Mature Secondary Structure
MRNSLYKKNIISISDLNQNELELVLKKSAFLKVKAQPNLLKNKIIASCFFEASTRTRLSF
CCCCCCCCCCEEEECCCCHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHHCCCCHHHHH
ETAVHRLGASIIGFSDGSNISLGKKGETLSDTISVISSYVDAIIIRHPQEGSARLAAQFS
HHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHEECC
NGVPIFNAGDGANQHPTQTLLDLFTIKETQNKLNNLNIAMVGDLKYGRTVHSLTQALAKY
CCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
KNNQFFFVSPDSLTMPNYINDMLYKKEIHWKRYKNIEEIISEIDILYMTRVQKERLDSTE
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHH
YASAKSKFVLQTSTLKNARNNLKILHPLPRIDEIDNNVDFTPYAWYFKQAANGIYARQAI
HHCCCCCEEEEHHHHHHHHCCCEEECCCCCCHHCCCCCCCCHHHHHHHHHHCCHHHHHHH
LSLVLIEKHFE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438