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The map label for this gene is pyrI

Identifier: 21672633

GI number: 21672633

Start: 408944

End: 409399

Strand: Direct

Name: pyrI

Synonym: BUsg358

Alternate gene names: 21672633

Gene position: 408944-409399 (Clockwise)

Preceding gene: 21672632

Following gene: 21672634

Centisome position: 63.75

GC content: 23.68

Gene sequence:

>456_bases
ATGCAAATAAATAAACTTCAAGTTGAAGCTATTAAATCTGGTAGTGTAATTGATCATATTCCAGCACATATTGGTTTTAA
ATTACTTTCATTATTCAGATTTACAGAAACAGAAAAAAGAATTACTATTGGATTAAATTTACCTTCTCAAAAATTAGATA
AAAAAGATATCATAAAAATTGAAAATACTTTTTTAAGCGATGATCAAATTAATCAATTAGCTATTTATGCACCATGTGCT
ACAGTTAATTATATTGAAAAATATAATTTAGTAGGAAAAATATTTCCTTCTTTACCTAAAAAGATAGATCGTATTCTAAT
TTGTCCCAACAGTAATTGTGCTAGTCACAATCATTTCATAACTTCTAGCTTTATTTTTACCAAAGATATAAATAATGAGA
TAAATTTAAAGTGTCAATATTGTGAAAAAGAATTCGCTAAAAACATAGTTTTATAA

Upstream 100 bases:

>100_bases
CCTTATGCTTGGTATTTTAAACAAGCAGCAAATGGAATTTATGCACGTCAAGCAATTTTATCATTAGTATTAATAGAAAA
ACATTTTGAATAAAAAATAT

Downstream 100 bases:

>100_bases
AAAACTTAAATATTACATATTTCATGTTTTTTTCTATTTAATAAAATTAAAAATGGTGCTTTATGAATGTTATAGTTAAT
ACTGAAAATGCCCCAAAACC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MQINKLQVEAIKSGSVIDHIPAHIGFKLLSLFRFTETEKRITIGLNLPSQKLDKKDIIKIENTFLSDDQINQLAIYAPCA
TVNYIEKYNLVGKIFPSLPKKIDRILICPNSNCASHNHFITSSFIFTKDINNEINLKCQYCEKEFAKNIVL

Sequences:

>Translated_151_residues
MQINKLQVEAIKSGSVIDHIPAHIGFKLLSLFRFTETEKRITIGLNLPSQKLDKKDIIKIENTFLSDDQINQLAIYAPCA
TVNYIEKYNLVGKIFPSLPKKIDRILICPNSNCASHNHFITSSFIFTKDINNEINLKCQYCEKEFAKNIVL
>Mature_151_residues
MQINKLQVEAIKSGSVIDHIPAHIGFKLLSLFRFTETEKRITIGLNLPSQKLDKKDIIKIENTFLSDDQINQLAIYAPCA
TVNYIEKYNLVGKIFPSLPKKIDRILICPNSNCASHNHFITSSFIFTKDINNEINLKCQYCEKEFAKNIVL

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=148, Percent_Identity=67.5675675675676, Blast_Score=211, Evalue=2e-56,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_BUCAP (Q8K9H8)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660700.1
- ProteinModelPortal:   Q8K9H8
- SMR:   Q8K9H8
- EnsemblBacteria:   EBBUCT00000000067
- GeneID:   1005797
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg358
- GeneTree:   EBGT00050000007873
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   BAPH198804:BUSG358-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17257; Mature: 17257

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQINKLQVEAIKSGSVIDHIPAHIGFKLLSLFRFTETEKRITIGLNLPSQKLDKKDIIKI
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCEEEE
ENTFLSDDQINQLAIYAPCATVNYIEKYNLVGKIFPSLPKKIDRILICPNSNCASHNHFI
ECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEE
TSSFIFTKDINNEINLKCQYCEKEFAKNIVL
EEEEEEEECCCCCEEEEEEHHHHHHHHCCCC
>Mature Secondary Structure
MQINKLQVEAIKSGSVIDHIPAHIGFKLLSLFRFTETEKRITIGLNLPSQKLDKKDIIKI
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCCEEEE
ENTFLSDDQINQLAIYAPCATVNYIEKYNLVGKIFPSLPKKIDRILICPNSNCASHNHFI
ECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCEE
TSSFIFTKDINNEINLKCQYCEKEFAKNIVL
EEEEEEEECCCCCEEEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438