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The map label for this gene is queF

Identifier: 21672566

GI number: 21672566

Start: 329919

End: 330707

Strand: Reverse

Name: queF

Synonym: BUsg288

Alternate gene names: 21672566

Gene position: 330707-329919 (Counterclockwise)

Preceding gene: 21672567

Following gene: 21672561

Centisome position: 51.56

GC content: 24.84

Gene sequence:

>789_bases
ATGAGTCTTAAAATAAATAATTTTAATTTTTTACGACCTATATCTAGAAAAAAACATCGAAAAAAAATTAAATTAAACTG
CCTTAATCTTCCTTTTAAAGGTAAAGATATTTGGACTTTATACGAATTATCTTGGCTAAATAAAAATGGATTGCCTCAAA
TTGCCATTGCTAAAATAGAAATTGATGTAAATAGCGCTAATATTATTGAATCTAAAAGTTTTAAAATTTATATAAATAGT
TTTAACCAAATGAAATTTAACAATAATATTGATTTTATTAATATATTAACTAATGATCTTACTAAGTGTATATGTGGACA
AATTTCTATTAAATTATTCAGTTTAGATGCAATTAAAAATGAAACAATTACAGATTTTCATGGCATATGCATAGATAATC
AAAATATTAAAATAGAATCATATAAATATACTCCATCATTCTTGATGATCAATTCTGAAAGAAAGATAATCAAAGAAGAT
CTATATACTCATTTATTTAAATCTAATTGTCCTGTCACCCAACAACCTGATTGGGCATCAATATATATTGCATATACTGG
ACTATCAATTAATCATGCATCTTTATTACGTTATTTGATTTCTTTTCGTTCTCATAACGAATTTCATGAAGAATGTATTG
AAAGAATTTTTAATGATATTAATAATATTTGCAAACCTGAAGAATTAAGTGTATACGCAAGATATACTCGAAGAGGCGGA
ATTGATATCAATCCATGGCGCAGTAATACTAATTTTTCGCCTTTTCTTACTCGATTAGCTAGACAATAA

Upstream 100 bases:

>100_bases
ATAAACAATATGATTTTATCCTAAATTAATATGTTGAATCAAAAAAAATCTCTATTATAACATAAATATAATCGTACACA
TTTTATATAAAAGAGAAACT

Downstream 100 bases:

>100_bases
ATAGTAGAAATAAAAAAAGCAGTATTAAAATTATACTTAATACTGCAATTTTAAAAAAATTAAATAAAATATGTTTTATA
TATACAATCATCTAACTATT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSLKINNFNFLRPISRKKHRKKIKLNCLNLPFKGKDIWTLYELSWLNKNGLPQIAIAKIEIDVNSANIIESKSFKIYINS
FNQMKFNNNIDFINILTNDLTKCICGQISIKLFSLDAIKNETITDFHGICIDNQNIKIESYKYTPSFLMINSERKIIKED
LYTHLFKSNCPVTQQPDWASIYIAYTGLSINHASLLRYLISFRSHNEFHEECIERIFNDINNICKPEELSVYARYTRRGG
IDINPWRSNTNFSPFLTRLARQ

Sequences:

>Translated_262_residues
MSLKINNFNFLRPISRKKHRKKIKLNCLNLPFKGKDIWTLYELSWLNKNGLPQIAIAKIEIDVNSANIIESKSFKIYINS
FNQMKFNNNIDFINILTNDLTKCICGQISIKLFSLDAIKNETITDFHGICIDNQNIKIESYKYTPSFLMINSERKIIKED
LYTHLFKSNCPVTQQPDWASIYIAYTGLSINHASLLRYLISFRSHNEFHEECIERIFNDINNICKPEELSVYARYTRRGG
IDINPWRSNTNFSPFLTRLARQ
>Mature_261_residues
SLKINNFNFLRPISRKKHRKKIKLNCLNLPFKGKDIWTLYELSWLNKNGLPQIAIAKIEIDVNSANIIESKSFKIYINSF
NQMKFNNNIDFINILTNDLTKCICGQISIKLFSLDAIKNETITDFHGICIDNQNIKIESYKYTPSFLMINSERKIIKEDL
YTHLFKSNCPVTQQPDWASIYIAYTGLSINHASLLRYLISFRSHNEFHEECIERIFNDINNICKPEELSVYARYTRRGGI
DINPWRSNTNFSPFLTRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=255, Percent_Identity=54.1176470588235, Blast_Score=306, Evalue=9e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_BUCAP (Q8K9N6)

Other databases:

- EMBL:   AE013218
- EMBL:   U11045
- RefSeq:   NP_660633.1
- ProteinModelPortal:   Q8K9N6
- SMR:   Q8K9N6
- EnsemblBacteria:   EBBUCT00000000498
- GeneID:   1005492
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg288
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- BioCyc:   BAPH198804:BUSG288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 30731; Mature: 30600

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKINNFNFLRPISRKKHRKKIKLNCLNLPFKGKDIWTLYELSWLNKNGLPQIAIAKIE
CEEEECCCCEECCHHHHHHCCEEEEEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEEE
IDVNSANIIESKSFKIYINSFNQMKFNNNIDFINILTNDLTKCICGQISIKLFSLDAIKN
EECCCCCEEECCCEEEEEECCCEEEECCCCCEEEEHHHHHHHHHHCCEEEEEEEEECCCC
ETITDFHGICIDNQNIKIESYKYTPSFLMINSERKIIKEDLYTHLFKSNCPVTQQPDWAS
CCCCCCCCEEECCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
IYIAYTGLSINHASLLRYLISFRSHNEFHEECIERIFNDINNICKPEELSVYARYTRRGG
EEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEEEHHCCC
IDINPWRSNTNFSPFLTRLARQ
CCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SLKINNFNFLRPISRKKHRKKIKLNCLNLPFKGKDIWTLYELSWLNKNGLPQIAIAKIE
EEEECCCCEECCHHHHHHCCEEEEEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEEE
IDVNSANIIESKSFKIYINSFNQMKFNNNIDFINILTNDLTKCICGQISIKLFSLDAIKN
EECCCCCEEECCCEEEEEECCCEEEECCCCCEEEEHHHHHHHHHHCCEEEEEEEEECCCC
ETITDFHGICIDNQNIKIESYKYTPSFLMINSERKIIKEDLYTHLFKSNCPVTQQPDWAS
CCCCCCCCEEECCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
IYIAYTGLSINHASLLRYLISFRSHNEFHEECIERIFNDINNICKPEELSVYARYTRRGG
EEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHEEEEEHHCCC
IDINPWRSNTNFSPFLTRLARQ
CCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438; 7765846