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The map label for this gene is bioB

Identifier: 21672559

GI number: 21672559

Start: 319167

End: 320195

Strand: Reverse

Name: bioB

Synonym: BUsg280

Alternate gene names: 21672559

Gene position: 320195-319167 (Counterclockwise)

Preceding gene: 21672561

Following gene: 21672554

Centisome position: 49.92

GC content: 28.28

Gene sequence:

>1029_bases
ATGAAACGAAAATGGAACCTAGAAGATACAAAAAAACTTTTTTCAAAACCCTTTTTTGATCTTATATTCGAAGCTCAAAA
AAAACACCGTGAATATTTTAATCCTAATAGAATACAAATTAGTACATTACTATCAATTAAAACTGGAGCGTGTCCAGAAG
ATTGCAAGTATTGTCCACAAAGTTCAAGATATAAAACTGGTTTAAAAAAAGAGCCGTTATTAGAAATAGAACAAATTTTA
AAAGCTGCAAAAAAAGCTAAATCTTCAGGTTCAACTCGATTTTGCATGGGTGCTGCATGGAAAAATCCTAAAGAAAAAGA
TATGCCATATTTAGAAAAAATTGTTAAAGAAGTAAAAAAAATGGGCATGGAAACCTGTATGACTTTAGGAACATTAAATA
ATTCTCAGGCAAAAAAATTAGCTAAAGCAGGTTTAGATTTTTATAATCATAATTTAGATACATCATCTAATTTTTATAGC
AGTATTATAACAACTCGTACATATGAAGAACGTTTAAGTACTTTAAAAAAGGTTCGAAATTCAGGTATGAAAATTTGTTC
TGGAGGAATTATTGGTCTTGGGGAAAAAAAACAAGATCGTATGGAACTTTTAATGGAATTATCTAATTTATCTATTCAAC
CAGAAAGTGTACCAATAAATATGTTAGTAAAAATACCAGGAACACCTATGGAAAAAAATGAAAATGTCGATCCATTTGAA
TTTATTAGAGTTATTGCAGCTACAAGAATTATGATGCCAAAATCTTATATTCGATTGTCTGCAGGTCGTGAAAACATGAG
TGATGAAACTCAAGCTATGTGTTTTATGGCAGGAGCAAATTCTATTTTTTATGGATGTAAATTATTAACTTCAAGTAATC
CGAAAGAAGAAAAAGATCAAAAACTTTTTGAAAAATTAGATCTTTTTCCTGATTACAAGATACAAAGCATTACAGAAGAA
AATAAAAATAATGAAAACTTAAAAATATCTGATGTAAATAAAGCACAATACTATAATGCGGCAATTTAA

Upstream 100 bases:

>100_bases
AATATATCAGATTGACTCATTATGATTTATATATAAAAATATTAAATAATTCATTATAAACTTTTAATTAAACAAAATCA
TATTTTTTTTGGAGATTAAT

Downstream 100 bases:

>100_bases
TAAACTAAATACAAAGATATTTTTTAAGAAATATTTTTATTTATTTTAAAATATCTATTTTTTAATTTGAGTAACCAACC
GTTATATTCTTTTGTATTAA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKRKWNLEDTKKLFSKPFFDLIFEAQKKHREYFNPNRIQISTLLSIKTGACPEDCKYCPQSSRYKTGLKKEPLLEIEQIL
KAAKKAKSSGSTRFCMGAAWKNPKEKDMPYLEKIVKEVKKMGMETCMTLGTLNNSQAKKLAKAGLDFYNHNLDTSSNFYS
SIITTRTYEERLSTLKKVRNSGMKICSGGIIGLGEKKQDRMELLMELSNLSIQPESVPINMLVKIPGTPMEKNENVDPFE
FIRVIAATRIMMPKSYIRLSAGRENMSDETQAMCFMAGANSIFYGCKLLTSSNPKEEKDQKLFEKLDLFPDYKIQSITEE
NKNNENLKISDVNKAQYYNAAI

Sequences:

>Translated_342_residues
MKRKWNLEDTKKLFSKPFFDLIFEAQKKHREYFNPNRIQISTLLSIKTGACPEDCKYCPQSSRYKTGLKKEPLLEIEQIL
KAAKKAKSSGSTRFCMGAAWKNPKEKDMPYLEKIVKEVKKMGMETCMTLGTLNNSQAKKLAKAGLDFYNHNLDTSSNFYS
SIITTRTYEERLSTLKKVRNSGMKICSGGIIGLGEKKQDRMELLMELSNLSIQPESVPINMLVKIPGTPMEKNENVDPFE
FIRVIAATRIMMPKSYIRLSAGRENMSDETQAMCFMAGANSIFYGCKLLTSSNPKEEKDQKLFEKLDLFPDYKIQSITEE
NKNNENLKISDVNKAQYYNAAI
>Mature_342_residues
MKRKWNLEDTKKLFSKPFFDLIFEAQKKHREYFNPNRIQISTLLSIKTGACPEDCKYCPQSSRYKTGLKKEPLLEIEQIL
KAAKKAKSSGSTRFCMGAAWKNPKEKDMPYLEKIVKEVKKMGMETCMTLGTLNNSQAKKLAKAGLDFYNHNLDTSSNFYS
SIITTRTYEERLSTLKKVRNSGMKICSGGIIGLGEKKQDRMELLMELSNLSIQPESVPINMLVKIPGTPMEKNENVDPFE
FIRVIAATRIMMPKSYIRLSAGRENMSDETQAMCFMAGANSIFYGCKLLTSSNPKEEKDQKLFEKLDLFPDYKIQSITEE
NKNNENLKISDVNKAQYYNAAI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=340, Percent_Identity=64.1176470588235, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6321725, Length=315, Percent_Identity=47.6190476190476, Blast_Score=318, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_BUCAP (Q8K9P1)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660626.1
- ProteinModelPortal:   Q8K9P1
- SMR:   Q8K9P1
- EnsemblBacteria:   EBBUCT00000000278
- GeneID:   1005484
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg280
- GeneTree:   EBGT00050000007949
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2300099
- BioCyc:   BAPH198804:BUSG280-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39090; Mature: 39090

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKWNLEDTKKLFSKPFFDLIFEAQKKHREYFNPNRIQISTLLSIKTGACPEDCKYCPQ
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHCCCC
SSRYKTGLKKEPLLEIEQILKAAKKAKSSGSTRFCMGAAWKNPKEKDMPYLEKIVKEVKK
CCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHH
MGMETCMTLGTLNNSQAKKLAKAGLDFYNHNLDTSSNFYSSIITTRTYEERLSTLKKVRN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGMKICSGGIIGLGEKKQDRMELLMELSNLSIQPESVPINMLVKIPGTPMEKNENVDPFE
CCCEEECCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
FIRVIAATRIMMPKSYIRLSAGRENMSDETQAMCFMAGANSIFYGCKLLTSSNPKEEKDQ
HHHHHHHHHHHCCHHHHEECCCCCCCCHHHHHHHHHHCCCHHHHHHEEECCCCCCHHHHH
KLFEKLDLFPDYKIQSITEENKNNENLKISDVNKAQYYNAAI
HHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHCCCC
>Mature Secondary Structure
MKRKWNLEDTKKLFSKPFFDLIFEAQKKHREYFNPNRIQISTLLSIKTGACPEDCKYCPQ
CCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHCCCC
SSRYKTGLKKEPLLEIEQILKAAKKAKSSGSTRFCMGAAWKNPKEKDMPYLEKIVKEVKK
CCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHH
MGMETCMTLGTLNNSQAKKLAKAGLDFYNHNLDTSSNFYSSIITTRTYEERLSTLKKVRN
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SGMKICSGGIIGLGEKKQDRMELLMELSNLSIQPESVPINMLVKIPGTPMEKNENVDPFE
CCCEEECCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
FIRVIAATRIMMPKSYIRLSAGRENMSDETQAMCFMAGANSIFYGCKLLTSSNPKEEKDQ
HHHHHHHHHHHCCHHHHEECCCCCCCCHHHHHHHHHHCCCHHHHHHEEECCCCCCHHHHH
KLFEKLDLFPDYKIQSITEENKNNENLKISDVNKAQYYNAAI
HHHHHHHCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438