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The map label for this gene is trpB

Identifier: 21672547

GI number: 21672547

Start: 302920

End: 304119

Strand: Reverse

Name: trpB

Synonym: BUsg267

Alternate gene names: 21672547

Gene position: 304119-302920 (Counterclockwise)

Preceding gene: 21672548

Following gene: 21672546

Centisome position: 47.41

GC content: 31.33

Gene sequence:

>1200_bases
ATGACTTTACTTAATCCTTATTTTGGCAAATTTGGAGGTATGTATGTTCCTCAAATATTAATGCCAGCTTTATATCAATT
AGAAAAAAATTTTGTTGACGCAAAAAAAGATTCAAATTTTCAAAAAAGTTTTTTTAACTATTTAAAGAATTATGCTGGAA
GACCAACTCCGCTCACTTTATGTAATAATTTAACTAATGGTACAAAAACACGTATTTATCTTAAAAGAGAAGACCTATTA
CATGGAGGAGCTCATAAAACTAATCAGGTATTAGGACAAGCAATGTTAGCAGTAAAAATGAAAAAAAAAGAAATAATTGC
TGAAACTGGTGCTGGTCAACATGGTGTTGCTGCTGCTATTGCTTCTGCATTATTTAATTTAAAATGTAAAATTTATATGG
GTTACAAAGATATAAAAAGACAAAGTCCGAATGTTTTTCGTATGAAATTAATGGGAGCAGAAGTCGTATCAGTAGAAAGT
GGATCAGGAACATTAAAAGATGCATGCAATGAAGCTTTACGTGATTGGTCTAGAAATTATCAAAAATCACATTATATGAT
TGGTACTGCTGCAGGTCCACATCCCTATCCCACAATTGTAAAAGAATTTCAAAAAATGATTGGAGAAGAGGCAAAAAAAC
AAATTCTAGAACAAGAAAATAGATTACCAGATGCAATAATTGCTTGTGTAGGTGGTGGGTCTAATGCAATTGGTATTTTT
TCTGACTTTATAGATGAAGACGTGAATTTAATTGGTGTGGAACCAGCTGGTCAGGGTATAGAAACAGGAAAACATGGAGC
ACCATTAAATCATGGTAGAACAGGTATTTATTTTGGAATGAAATCACATTTAATGCAAAGTCAAGAAGGTCAAATAGAAA
AATCTTGGTCTATTTCTGCTGGTTTAGATTTTCCTTCTGTTGGTCCTGAACATTCTTGGTTAAATAGTATTCATCGTGCT
AAATATGTATCCATTACTGATATAGAAGCTTTAGAAGCTTTCCAAATTTTATCTAAAAAAGAAGGTATTATTCCTGCACT
AGAGTCTTCTCATGCATTAGCTTATGCATTAAAATTAATGTATCTAGATCCTAAAAAAGAACAAGTATTTATTGTGAATC
TTTCTGGACGTGGAGACAAAGATATATTTACAGTTCGTGAGATTTTAAAAAAAACGGAAAAAAAACATGAGTCGTTATAA

Upstream 100 bases:

>100_bases
AAATCTCCTGGCATCAAAGATCACAAAAAAATTAGTTTAATTTTTAAAAAACTAACTTTTTAATCAATTATATTAAAAAC
ATATTGGAAATCAACGAAAA

Downstream 100 bases:

>100_bases
AAAAATGTTTCAAAAATTATCTTTTCTTAAAGAAGGATGTTTTGTACCTTTCGTAGTGATAGGAGATCCTTCTTTAGAAA
TGTCATTAAAAATTATTGAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTLLNPYFGKFGGMYVPQILMPALYQLEKNFVDAKKDSNFQKSFFNYLKNYAGRPTPLTLCNNLTNGTKTRIYLKREDLL
HGGAHKTNQVLGQAMLAVKMKKKEIIAETGAGQHGVAAAIASALFNLKCKIYMGYKDIKRQSPNVFRMKLMGAEVVSVES
GSGTLKDACNEALRDWSRNYQKSHYMIGTAAGPHPYPTIVKEFQKMIGEEAKKQILEQENRLPDAIIACVGGGSNAIGIF
SDFIDEDVNLIGVEPAGQGIETGKHGAPLNHGRTGIYFGMKSHLMQSQEGQIEKSWSISAGLDFPSVGPEHSWLNSIHRA
KYVSITDIEALEAFQILSKKEGIIPALESSHALAYALKLMYLDPKKEQVFIVNLSGRGDKDIFTVREILKKTEKKHESL

Sequences:

>Translated_399_residues
MTLLNPYFGKFGGMYVPQILMPALYQLEKNFVDAKKDSNFQKSFFNYLKNYAGRPTPLTLCNNLTNGTKTRIYLKREDLL
HGGAHKTNQVLGQAMLAVKMKKKEIIAETGAGQHGVAAAIASALFNLKCKIYMGYKDIKRQSPNVFRMKLMGAEVVSVES
GSGTLKDACNEALRDWSRNYQKSHYMIGTAAGPHPYPTIVKEFQKMIGEEAKKQILEQENRLPDAIIACVGGGSNAIGIF
SDFIDEDVNLIGVEPAGQGIETGKHGAPLNHGRTGIYFGMKSHLMQSQEGQIEKSWSISAGLDFPSVGPEHSWLNSIHRA
KYVSITDIEALEAFQILSKKEGIIPALESSHALAYALKLMYLDPKKEQVFIVNLSGRGDKDIFTVREILKKTEKKHESL
>Mature_398_residues
TLLNPYFGKFGGMYVPQILMPALYQLEKNFVDAKKDSNFQKSFFNYLKNYAGRPTPLTLCNNLTNGTKTRIYLKREDLLH
GGAHKTNQVLGQAMLAVKMKKKEIIAETGAGQHGVAAAIASALFNLKCKIYMGYKDIKRQSPNVFRMKLMGAEVVSVESG
SGTLKDACNEALRDWSRNYQKSHYMIGTAAGPHPYPTIVKEFQKMIGEEAKKQILEQENRLPDAIIACVGGGSNAIGIFS
DFIDEDVNLIGVEPAGQGIETGKHGAPLNHGRTGIYFGMKSHLMQSQEGQIEKSWSISAGLDFPSVGPEHSWLNSIHRAK
YVSITDIEALEAFQILSKKEGIIPALESSHALAYALKLMYLDPKKEQVFIVNLSGRGDKDIFTVREILKKTEKKHESL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=390, Percent_Identity=75.8974358974359, Blast_Score=629, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321412, Length=387, Percent_Identity=49.0956072351421, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_BUCAP (P42391)

Other databases:

- EMBL:   Z19055
- EMBL:   AE013218
- PIR:   C49897
- RefSeq:   NP_660614.1
- ProteinModelPortal:   P42391
- SMR:   P42391
- EnsemblBacteria:   EBBUCT00000000427
- GeneID:   1005469
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg267
- GeneTree:   EBGT00050000008214
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   BAPH198804:BUSG267-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 44248; Mature: 44116

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLNPYFGKFGGMYVPQILMPALYQLEKNFVDAKKDSNFQKSFFNYLKNYAGRPTPLTL
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHH
CNNLTNGTKTRIYLKREDLLHGGAHKTNQVLGQAMLAVKMKKKEIIAETGAGQHGVAAAI
HHCCCCCCEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ASALFNLKCKIYMGYKDIKRQSPNVFRMKLMGAEVVSVESGSGTLKDACNEALRDWSRNY
HHHHHCCEEEEEECHHHHHCCCCCEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHH
QKSHYMIGTAAGPHPYPTIVKEFQKMIGEEAKKQILEQENRLPDAIIACVGGGSNAIGIF
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
SDFIDEDVNLIGVEPAGQGIETGKHGAPLNHGRTGIYFGMKSHLMQSQEGQIEKSWSISA
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCC
GLDFPSVGPEHSWLNSIHRAKYVSITDIEALEAFQILSKKEGIIPALESSHALAYALKLM
CCCCCCCCCCHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YLDPKKEQVFIVNLSGRGDKDIFTVREILKKTEKKHESL
EECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLLNPYFGKFGGMYVPQILMPALYQLEKNFVDAKKDSNFQKSFFNYLKNYAGRPTPLTL
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHH
CNNLTNGTKTRIYLKREDLLHGGAHKTNQVLGQAMLAVKMKKKEIIAETGAGQHGVAAAI
HHCCCCCCEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ASALFNLKCKIYMGYKDIKRQSPNVFRMKLMGAEVVSVESGSGTLKDACNEALRDWSRNY
HHHHHCCEEEEEECHHHHHCCCCCEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHH
QKSHYMIGTAAGPHPYPTIVKEFQKMIGEEAKKQILEQENRLPDAIIACVGGGSNAIGIF
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
SDFIDEDVNLIGVEPAGQGIETGKHGAPLNHGRTGIYFGMKSHLMQSQEGQIEKSWSISA
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCC
GLDFPSVGPEHSWLNSIHRAKYVSITDIEALEAFQILSKKEGIIPALESSHALAYALKLM
CCCCCCCCCCHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
YLDPKKEQVFIVNLSGRGDKDIFTVREILKKTEKKHESL
EECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8407819; 12089438