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The map label for this gene is trpA

Identifier: 21672546

GI number: 21672546

Start: 302118

End: 302933

Strand: Reverse

Name: trpA

Synonym: BUsg266

Alternate gene names: 21672546

Gene position: 302933-302118 (Counterclockwise)

Preceding gene: 21672547

Following gene: 21672545

Centisome position: 47.23

GC content: 25.98

Gene sequence:

>816_bases
ATGAGTCGTTATAAAAAAATGTTTCAAAAATTATCTTTTCTTAAAGAAGGATGTTTTGTACCTTTCGTAGTGATAGGAGA
TCCTTCTTTAGAAATGTCATTAAAAATTATTGAAACACTGATTGAAAATGGAGCAGATGCTTTAGAATTAGGAATTCCAT
TTTCAGATCCATTAGCAGATGGTCCTATTATTCAAAAAGCAAGTCTGCGTGCATTATATAAAAAAAACACTTTTTTACAA
TGTTTTGAACTCATTGAGAATATACGTAATAAGTATTTGCATTTACCTATTGGTATCTTATTATATGCCAATTTAGTATA
CAATTACGGTATTAAAAACTTCTATTATCAATGTTTTCGATCTGGTTTGGATTCTGTATTAATAGCAGATATACCTATAG
AAGAATATGAACCTTTTTACAAGATAGCAAATCAATATAATATTAATTCTATTTTTATATGTCCTCCTGATGCAGACGAT
TCACTTTTATCTAAAATTTCTTTATATGCAAAGGGATATATTTATTTATTATCTCGTCCAGGAGTAACTGGGATAGAAAA
AAAAACAATATCATTGTCAAATATATTTATAGAAAAAATAAAAAAATATAATTCTCTTCCACTACTACAAGGATTTGGAA
TTGAAAACCCTACACAAATTCAAAAATCGATTTTATCTGGTACTAATGGTGTAATATGTGGTTCAGCAATTATCGATATA
ATCGAAAAAAATTTAAACAAAGAAAAAAAAATGATAAATGAGATAAAAAATTTTACTAGTAAATTAAAAAAAGCTACTAA
ATTCGTACAATCTTAG

Upstream 100 bases:

>100_bases
GATCCTAAAAAAGAACAAGTATTTATTGTGAATCTTTCTGGACGTGGAGACAAAGATATATTTACAGTTCGTGAGATTTT
AAAAAAAACGGAAAAAAAAC

Downstream 100 bases:

>100_bases
TTTATAAAAACTTAAATTTTATCTATCTTTAATAAATTTATTGAAAAAAATTTTATCTAAAAAAATGTGTATAATCTTTA
AATTTTATACAATTTTTATT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSRYKKMFQKLSFLKEGCFVPFVVIGDPSLEMSLKIIETLIENGADALELGIPFSDPLADGPIIQKASLRALYKKNTFLQ
CFELIENIRNKYLHLPIGILLYANLVYNYGIKNFYYQCFRSGLDSVLIADIPIEEYEPFYKIANQYNINSIFICPPDADD
SLLSKISLYAKGYIYLLSRPGVTGIEKKTISLSNIFIEKIKKYNSLPLLQGFGIENPTQIQKSILSGTNGVICGSAIIDI
IEKNLNKEKKMINEIKNFTSKLKKATKFVQS

Sequences:

>Translated_271_residues
MSRYKKMFQKLSFLKEGCFVPFVVIGDPSLEMSLKIIETLIENGADALELGIPFSDPLADGPIIQKASLRALYKKNTFLQ
CFELIENIRNKYLHLPIGILLYANLVYNYGIKNFYYQCFRSGLDSVLIADIPIEEYEPFYKIANQYNINSIFICPPDADD
SLLSKISLYAKGYIYLLSRPGVTGIEKKTISLSNIFIEKIKKYNSLPLLQGFGIENPTQIQKSILSGTNGVICGSAIIDI
IEKNLNKEKKMINEIKNFTSKLKKATKFVQS
>Mature_270_residues
SRYKKMFQKLSFLKEGCFVPFVVIGDPSLEMSLKIIETLIENGADALELGIPFSDPLADGPIIQKASLRALYKKNTFLQC
FELIENIRNKYLHLPIGILLYANLVYNYGIKNFYYQCFRSGLDSVLIADIPIEEYEPFYKIANQYNINSIFICPPDADDS
LLSKISLYAKGYIYLLSRPGVTGIEKKTISLSNIFIEKIKKYNSLPLLQGFGIENPTQIQKSILSGTNGVICGSAIIDII
EKNLNKEKKMINEIKNFTSKLKKATKFVQS

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=55.0561797752809, Blast_Score=303, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6321412, Length=227, Percent_Identity=33.0396475770925, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BUCAP (P42389)

Other databases:

- EMBL:   Z19055
- EMBL:   AE013218
- PIR:   D49897
- RefSeq:   NP_660613.1
- ProteinModelPortal:   P42389
- SMR:   P42389
- EnsemblBacteria:   EBBUCT00000000133
- GeneID:   1005468
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg266
- GeneTree:   EBGT00050000007798
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   BAPH198804:BUSG266-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 30685; Mature: 30554

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYKKMFQKLSFLKEGCFVPFVVIGDPSLEMSLKIIETLIENGADALELGIPFSDPLAD
CCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPIIQKASLRALYKKNTFLQCFELIENIRNKYLHLPIGILLYANLVYNYGIKNFYYQCFR
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCHHHHHHHHHH
SGLDSVLIADIPIEEYEPFYKIANQYNINSIFICPPDADDSLLSKISLYAKGYIYLLSRP
HCCCCEEEECCCHHHCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEECC
GVTGIEKKTISLSNIFIEKIKKYNSLPLLQGFGIENPTQIQKSILSGTNGVICGSAIIDI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHH
IEKNLNKEKKMINEIKNFTSKLKKATKFVQS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRYKKMFQKLSFLKEGCFVPFVVIGDPSLEMSLKIIETLIENGADALELGIPFSDPLAD
CHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPIIQKASLRALYKKNTFLQCFELIENIRNKYLHLPIGILLYANLVYNYGIKNFYYQCFR
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHCHHHHHHHHHH
SGLDSVLIADIPIEEYEPFYKIANQYNINSIFICPPDADDSLLSKISLYAKGYIYLLSRP
HCCCCEEEECCCHHHCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEECC
GVTGIEKKTISLSNIFIEKIKKYNSLPLLQGFGIENPTQIQKSILSGTNGVICGSAIIDI
CCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHH
IEKNLNKEKKMINEIKNFTSKLKKATKFVQS
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407819; 12089438