Click here to switch to the map view.

The map label for this gene is lepA

Identifier: 21672532

GI number: 21672532

Start: 285864

End: 287669

Strand: Reverse

Name: lepA

Synonym: BUsg251

Alternate gene names: 21672532

Gene position: 287669-285864 (Counterclockwise)

Preceding gene: 21672542

Following gene: 21672531

Centisome position: 44.85

GC content: 28.29

Gene sequence:

>1806_bases
ATGGTCAAAAAAATGAAAAATATAAGAAACTTTTCTATTATCGCTCATATTGATCATGGAAAATCAACTTTATCAGATCG
TTTGATTCAAAAATGCGGTGGACTTTCTGAAAGAGAAATGTCTGATCAAGTTTTAGATTCTATGGATTTAGAAAAAGAAA
GAGGTATAACAATTAAAGCACAAAGTGTAATGATTGATTATAAAGATAAAAATGGCAATGTTTTTCATTTAAATTTTATT
GATACACCTGGTCATGTAAATTTTTCATACGAAGTTTCTAGATCATTAGCAGCGTGTGAGGGGGCGTTATTAGTTGTTGA
TTCAAGTCAAGGGGTAGAAGCACAAACATTAGCCAATTGTGCAACCGCTTTAGAAATGAAACTATCAATAGTTCCAGTAT
TAAATAAAATAGATTTACCTAATTCAAATCCTGAAAAGGTAGCGAGAGAAATTAAAGAAATTATTGGTATTTCTGCATTA
GATGCAATTAGATGTTCAGCTAAAACTGGTCAAGGAATAGAAGAATTAATAGAACAAATCATTAAAAAAATTCCAGCGCC
TGATGGTGATGAAAAAAAACCATTACAAGCACTTATTATCGATTCTTGGTTTGATAATTATCTAGGTGTAGTATCTTTAA
TAAGAATTAAAAATGGAATAATTTCAAAAAAAGATAAGATTAAAGTGATGAGTACAGGAAAAACATATTTTGTTGATCAT
TTAGGTATATTCACGCCTAAAAAAATAAATAAAAACTATTTAAAATGCGGCGAAGTAGGTTGGATTGTTTGTGGCATTAA
AAACATTTCTGCCGCTCCAGTTGGAGATACATTAACTAATGCCAAAAATCCAGCAATGAATATGCTAACAGGTTTTAAAA
AAATTAAACCTCAGATTTATGCTGGTTTATTTCCTGTTACATCAGATCAATATGAAATTTTCAGAGATGCTTTAGGAAAA
CTAAGTTTAAATGACTCTTCATTATTTTATGAACCAATAAATTCAACTGCTCTTGGTTTTGGATTTCGATGTGGTTTTTT
AGGTTTACTACATATGGAAATTGTCCAAGCACGTTTAGAACGAGAATATTCTCTTAATCTAATTTCAACAGCTCCGAATG
TAATTTATCAAATAGAATTCAATAATGGTGAAAAAATTTATTTAGATACTCCTTCAAATTTTCCTGTTGACAATAAAATA
AGAAAAATTAAAGAACCCATTGTTGAATGTAATATTCTATTACCACCTAAATTTCTCGGTCCAGTCATGAAATTATGTAT
AAAAAAAAGAGGAACAGAAATTAATATGATTTATCATGAACAGCAAGTATCTTTGAAATATCATATTCCAATGAATGAAG
TTGTTTTGAATTTTTTTGATGAATTAAAGTCAGTTTCAAGTGGATATGCTTCATTAGAATATGATTTTAAATATTTTAAA
ACTGTTAAAATGGTTAGAATAGACATTCTAATCAATTCAGAAAGAGTAGATGCACTGACAGTAATTTCTTATTATAAGAA
TGCTCAAAATCGTGCTCGTGAAATAGTTAATAAAATGAAAGAATTAATACCCCGTCATCAATTTGATATTAATATTCAAG
CAACAATTAACAATTCTATTGTTGCTAGATCAACGATTAAGCAATTAAGAAAAAATGTGTTAGCAAAGTGTTATGGAGGT
GATATTAGTAGAAAAAAGAAACTATTGAAAAAACAAAAAGATGGTAAGAAAAGAATGAAAAAAATAGGAAACGTCAATAT
GCCAAAAACAGCTTTTCTTGACATTTTAAATATCCATAAGGATTAA

Upstream 100 bases:

>100_bases
TAAGAAATATATTAAATATTTATACTTTTAAAAAATATTAATATAAAATAAATTTTGAATATACCTTTTAACTACACAAT
TTAAATAAATTAATTTTAAA

Downstream 100 bases:

>100_bases
AACATAAGGAGAAATAATGCGTAATATATTGACTATTTTTTTATTAACAAGCACTTTTTTTACAGGTATATTATGGATCA
TTGATCATATTCTATTAATT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MVKKMKNIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSDQVLDSMDLEKERGITIKAQSVMIDYKDKNGNVFHLNFI
DTPGHVNFSYEVSRSLAACEGALLVVDSSQGVEAQTLANCATALEMKLSIVPVLNKIDLPNSNPEKVAREIKEIIGISAL
DAIRCSAKTGQGIEELIEQIIKKIPAPDGDEKKPLQALIIDSWFDNYLGVVSLIRIKNGIISKKDKIKVMSTGKTYFVDH
LGIFTPKKINKNYLKCGEVGWIVCGIKNISAAPVGDTLTNAKNPAMNMLTGFKKIKPQIYAGLFPVTSDQYEIFRDALGK
LSLNDSSLFYEPINSTALGFGFRCGFLGLLHMEIVQARLEREYSLNLISTAPNVIYQIEFNNGEKIYLDTPSNFPVDNKI
RKIKEPIVECNILLPPKFLGPVMKLCIKKRGTEINMIYHEQQVSLKYHIPMNEVVLNFFDELKSVSSGYASLEYDFKYFK
TVKMVRIDILINSERVDALTVISYYKNAQNRAREIVNKMKELIPRHQFDINIQATINNSIVARSTIKQLRKNVLAKCYGG
DISRKKKLLKKQKDGKKRMKKIGNVNMPKTAFLDILNIHKD

Sequences:

>Translated_601_residues
MVKKMKNIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSDQVLDSMDLEKERGITIKAQSVMIDYKDKNGNVFHLNFI
DTPGHVNFSYEVSRSLAACEGALLVVDSSQGVEAQTLANCATALEMKLSIVPVLNKIDLPNSNPEKVAREIKEIIGISAL
DAIRCSAKTGQGIEELIEQIIKKIPAPDGDEKKPLQALIIDSWFDNYLGVVSLIRIKNGIISKKDKIKVMSTGKTYFVDH
LGIFTPKKINKNYLKCGEVGWIVCGIKNISAAPVGDTLTNAKNPAMNMLTGFKKIKPQIYAGLFPVTSDQYEIFRDALGK
LSLNDSSLFYEPINSTALGFGFRCGFLGLLHMEIVQARLEREYSLNLISTAPNVIYQIEFNNGEKIYLDTPSNFPVDNKI
RKIKEPIVECNILLPPKFLGPVMKLCIKKRGTEINMIYHEQQVSLKYHIPMNEVVLNFFDELKSVSSGYASLEYDFKYFK
TVKMVRIDILINSERVDALTVISYYKNAQNRAREIVNKMKELIPRHQFDINIQATINNSIVARSTIKQLRKNVLAKCYGG
DISRKKKLLKKQKDGKKRMKKIGNVNMPKTAFLDILNIHKD
>Mature_601_residues
MVKKMKNIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSDQVLDSMDLEKERGITIKAQSVMIDYKDKNGNVFHLNFI
DTPGHVNFSYEVSRSLAACEGALLVVDSSQGVEAQTLANCATALEMKLSIVPVLNKIDLPNSNPEKVAREIKEIIGISAL
DAIRCSAKTGQGIEELIEQIIKKIPAPDGDEKKPLQALIIDSWFDNYLGVVSLIRIKNGIISKKDKIKVMSTGKTYFVDH
LGIFTPKKINKNYLKCGEVGWIVCGIKNISAAPVGDTLTNAKNPAMNMLTGFKKIKPQIYAGLFPVTSDQYEIFRDALGK
LSLNDSSLFYEPINSTALGFGFRCGFLGLLHMEIVQARLEREYSLNLISTAPNVIYQIEFNNGEKIYLDTPSNFPVDNKI
RKIKEPIVECNILLPPKFLGPVMKLCIKKRGTEINMIYHEQQVSLKYHIPMNEVVLNFFDELKSVSSGYASLEYDFKYFK
TVKMVRIDILINSERVDALTVISYYKNAQNRAREIVNKMKELIPRHQFDINIQATINNSIVARSTIKQLRKNVLAKCYGG
DISRKKKLLKKQKDGKKRMKKIGNVNMPKTAFLDILNIHKD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=45.4242928452579, Blast_Score=560, Evalue=1e-159,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=40.6666666666667, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=42.3357664233577, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI18390331, Length=156, Percent_Identity=38.4615384615385, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI25306283, Length=197, Percent_Identity=36.5482233502538, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI19923640, Length=197, Percent_Identity=36.5482233502538, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI25306287, Length=197, Percent_Identity=36.5482233502538, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=36.5671641791045, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=36.5671641791045, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI310132016, Length=114, Percent_Identity=39.4736842105263, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI310110807, Length=114, Percent_Identity=39.4736842105263, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI310123363, Length=114, Percent_Identity=39.4736842105263, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=70.6867671691792, Blast_Score=884, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=516, Percent_Identity=28.1007751937984, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789738, Length=153, Percent_Identity=34.640522875817, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790835, Length=151, Percent_Identity=31.1258278145695, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789559, Length=230, Percent_Identity=26.5217391304348, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789737, Length=278, Percent_Identity=27.3381294964029, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790412, Length=278, Percent_Identity=27.3381294964029, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=38.9525368248772, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17556745, Length=156, Percent_Identity=34.6153846153846, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=231, Percent_Identity=32.9004329004329, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=169, Percent_Identity=34.3195266272189, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=35.3383458646617, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71988811, Length=189, Percent_Identity=30.1587301587302, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=189, Percent_Identity=30.1587301587302, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=29.4605809128631, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=42.6910299003322, Blast_Score=492, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=42, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=42, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323098, Length=228, Percent_Identity=32.4561403508772, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=36.5217391304348, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=150, Percent_Identity=38, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=246, Percent_Identity=26.4227642276423, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=41.0981697171381, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=39.622641509434, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=37.012987012987, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=37.7483443708609, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=37.7483443708609, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28574573, Length=141, Percent_Identity=43.2624113475177, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.255033557047, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=34.8148148148148, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19921738, Length=233, Percent_Identity=29.6137339055794, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28572034, Length=226, Percent_Identity=27.8761061946903, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_BUCAP (Q8K9Q9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660599.1
- ProteinModelPortal:   Q8K9Q9
- SMR:   Q8K9Q9
- EnsemblBacteria:   EBBUCT00000000098
- GeneID:   1005453
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg251
- GeneTree:   EBGT00050000007783
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   BAPH198804:BUSG251-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67749; Mature: 67749

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKMKNIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSDQVLDSMDLEKERGITIKA
CCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEE
QSVMIDYKDKNGNVFHLNFIDTPGHVNFSYEVSRSLAACEGALLVVDSSQGVEAQTLANC
EEEEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
ATALEMKLSIVPVLNKIDLPNSNPEKVAREIKEIIGISALDAIRCSAKTGQGIEELIEQI
HHHHHHHEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHH
IKKIPAPDGDEKKPLQALIIDSWFDNYLGVVSLIRIKNGIISKKDKIKVMSTGKTYFVDH
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEHH
LGIFTPKKINKNYLKCGEVGWIVCGIKNISAAPVGDTLTNAKNPAMNMLTGFKKIKPQIY
CCCCCCCCCCCCHHEECCCCEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCHHHH
AGLFPVTSDQYEIFRDALGKLSLNDSSLFYEPINSTALGFGFRCGFLGLLHMEIVQARLE
CCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHH
REYSLNLISTAPNVIYQIEFNNGEKIYLDTPSNFPVDNKIRKIKEPIVECNILLPPKFLG
HHHCCEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCHHHHH
PVMKLCIKKRGTEINMIYHEQQVSLKYHIPMNEVVLNFFDELKSVSSGYASLEYDFKYFK
HHHHHHHHHCCCEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECHHHHH
TVKMVRIDILINSERVDALTVISYYKNAQNRAREIVNKMKELIPRHQFDINIQATINNSI
EEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCC
VARSTIKQLRKNVLAKCYGGDISRKKKLLKKQKDGKKRMKKIGNVNMPKTAFLDILNIHK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
D
C
>Mature Secondary Structure
MVKKMKNIRNFSIIAHIDHGKSTLSDRLIQKCGGLSEREMSDQVLDSMDLEKERGITIKA
CCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEE
QSVMIDYKDKNGNVFHLNFIDTPGHVNFSYEVSRSLAACEGALLVVDSSQGVEAQTLANC
EEEEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
ATALEMKLSIVPVLNKIDLPNSNPEKVAREIKEIIGISALDAIRCSAKTGQGIEELIEQI
HHHHHHHEEEEEHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHH
IKKIPAPDGDEKKPLQALIIDSWFDNYLGVVSLIRIKNGIISKKDKIKVMSTGKTYFVDH
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEHH
LGIFTPKKINKNYLKCGEVGWIVCGIKNISAAPVGDTLTNAKNPAMNMLTGFKKIKPQIY
CCCCCCCCCCCCHHEECCCCEEEEECCCCCCCCCCCHHCCCCCCHHHHHHHHHHHCHHHH
AGLFPVTSDQYEIFRDALGKLSLNDSSLFYEPINSTALGFGFRCGFLGLLHMEIVQARLE
CCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHH
REYSLNLISTAPNVIYQIEFNNGEKIYLDTPSNFPVDNKIRKIKEPIVECNILLPPKFLG
HHHCCEEEECCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCHHHHH
PVMKLCIKKRGTEINMIYHEQQVSLKYHIPMNEVVLNFFDELKSVSSGYASLEYDFKYFK
HHHHHHHHHCCCEEEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECHHHHH
TVKMVRIDILINSERVDALTVISYYKNAQNRAREIVNKMKELIPRHQFDINIQATINNSI
EEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCC
VARSTIKQLRKNVLAKCYGGDISRKKKLLKKQKDGKKRMKKIGNVNMPKTAFLDILNIHK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12089438