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The map label for this gene is cls

Identifier: 21672542

GI number: 21672542

Start: 298432

End: 299892

Strand: Reverse

Name: cls

Synonym: BUsg262

Alternate gene names: 21672542

Gene position: 299892-298432 (Counterclockwise)

Preceding gene: 21672543

Following gene: 21672532

Centisome position: 46.75

GC content: 27.93

Gene sequence:

>1461_bases
ATGGATATTTTCTATGATTTAATAAAATGGCTAGTTGTCTTAATATACTGGTTATTAATTGCTAATATTACCTATCGTAT
TTTAATTAAACGTCGTAGTATACCCTCCTCCATGTCTTGGCTTTTGACTATTTATATAATTCCATTTATAGGAATAGCTA
TTTGGTTTTTCTTTGGCGAATTGTATTTAGGAAAAAGACAAAAGAAAATAGCAAAAAAAATTTGGTCTATATCTAATACA
TATCTTAATAAACTTAAATCTTATAAATATATATTTCAAATAAAAAATAGTGAAGTAGCTACTTCTTTATTTCAATTGTG
CAAGCATCGGCAAGGAATTTATGGTATTAAAAATAATAAAATAACTCTTTTAACTAACACTCAAAATACTATTGAAGTTT
TAATACGCGACATTTATTCAGCTCGTAACAATATTGAAATGGTATTTTATATTTGGAAACCAGGAGGTATTGCTGATGAT
GTAGCCATGGCTTTAATAGATTCCGCTAAACGTGGAGTACACTGTAGGCTTATGTTAGATTCAGCAGGAAGTGTAGAATT
TTTTAGAAGTCCTTGGTTTGACATTATGAAAAAATCTGGAATCGAAATTGTAGAAGCATTAAAAGTTAGTTTAATAAGAA
TTTTTTTGAGGCGTTTAGATGTAAGACAACACAGAAAAATTATACTAATTGATAATTATATTTCTTATTCTGGAAGTATG
AATCTTGTAGATCCATACTTATTTAAACAATCCTCAGGTGTAGGTCAATGGATTGATTTAATGACAAGAATAGAAGGTCC
TGTAGCTACAGCTATAGGTATAATTTATTCATGTGATTGGGAAATTGAAACAGGTTTTAAAATTTTACCTAAATTACCAA
ATAAAGAAATGCTAAAAAAAAAATGCAACCAAAACTCTAGTATTCAAGTAATTGCATCAGGTCCAGGATTTCCTAAAAAT
GTAATTCATCAAGCGTTATTAACTGCTATTTATTCAGCACGAAATGAATTAATTATGACAACTCCATATCTTGTACCTAG
TGATGATTTACTACATGCTATTTGTACTGCTGCTCAAAGAGGTGTAAAAGTTAGCATTATCATACCTTTGTACCATGATT
CTATTTTAGTTAAATGGGCAAGTAGAGTATTTTTTAGTGAATTATTAGAAGCAGGAGTAAAAATATATCAATTTAAAAAA
GGATTATTACATAGTAAGAGTATACTAATTGATCAACAACTTAGTTTGATTGGTACTGTAAATTTAGACATGAGAAGCTT
ATGGTTAAATTTCGAAATTACCTTAGTAATTGACGATAGGAAATTTAGCCATCATTTATCTGCTATACAAAAAGAATATA
TTGATAATTCTCGATTATTAGATAAAAATACTTGGTCTATGCGCGCATATTGGACTAGAATTTTAGAAAAAATTTTTTAT
TTTTTAAGTCCGTTATTATAA

Upstream 100 bases:

>100_bases
AACAGACTATTAGTTAATTTTTCATATTTAAATAGTTAAAAAATATTTAATTTAATATAAAATAATTTTATAAAAAGGTA
TGAATAGGACCTTCAAATGA

Downstream 100 bases:

>100_bases
TTTAAAAAAAACTTTTAGGGGGTTCATTAAAAAACCCCTGATATTTAAAAAAAAATAAAAAATATTTAAAACTCTTCATA
CTGCATAAATGAAAATTTTT

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MDIFYDLIKWLVVLIYWLLIANITYRILIKRRSIPSSMSWLLTIYIIPFIGIAIWFFFGELYLGKRQKKIAKKIWSISNT
YLNKLKSYKYIFQIKNSEVATSLFQLCKHRQGIYGIKNNKITLLTNTQNTIEVLIRDIYSARNNIEMVFYIWKPGGIADD
VAMALIDSAKRGVHCRLMLDSAGSVEFFRSPWFDIMKKSGIEIVEALKVSLIRIFLRRLDVRQHRKIILIDNYISYSGSM
NLVDPYLFKQSSGVGQWIDLMTRIEGPVATAIGIIYSCDWEIETGFKILPKLPNKEMLKKKCNQNSSIQVIASGPGFPKN
VIHQALLTAIYSARNELIMTTPYLVPSDDLLHAICTAAQRGVKVSIIIPLYHDSILVKWASRVFFSELLEAGVKIYQFKK
GLLHSKSILIDQQLSLIGTVNLDMRSLWLNFEITLVIDDRKFSHHLSAIQKEYIDNSRLLDKNTWSMRAYWTRILEKIFY
FLSPLL

Sequences:

>Translated_486_residues
MDIFYDLIKWLVVLIYWLLIANITYRILIKRRSIPSSMSWLLTIYIIPFIGIAIWFFFGELYLGKRQKKIAKKIWSISNT
YLNKLKSYKYIFQIKNSEVATSLFQLCKHRQGIYGIKNNKITLLTNTQNTIEVLIRDIYSARNNIEMVFYIWKPGGIADD
VAMALIDSAKRGVHCRLMLDSAGSVEFFRSPWFDIMKKSGIEIVEALKVSLIRIFLRRLDVRQHRKIILIDNYISYSGSM
NLVDPYLFKQSSGVGQWIDLMTRIEGPVATAIGIIYSCDWEIETGFKILPKLPNKEMLKKKCNQNSSIQVIASGPGFPKN
VIHQALLTAIYSARNELIMTTPYLVPSDDLLHAICTAAQRGVKVSIIIPLYHDSILVKWASRVFFSELLEAGVKIYQFKK
GLLHSKSILIDQQLSLIGTVNLDMRSLWLNFEITLVIDDRKFSHHLSAIQKEYIDNSRLLDKNTWSMRAYWTRILEKIFY
FLSPLL
>Mature_486_residues
MDIFYDLIKWLVVLIYWLLIANITYRILIKRRSIPSSMSWLLTIYIIPFIGIAIWFFFGELYLGKRQKKIAKKIWSISNT
YLNKLKSYKYIFQIKNSEVATSLFQLCKHRQGIYGIKNNKITLLTNTQNTIEVLIRDIYSARNNIEMVFYIWKPGGIADD
VAMALIDSAKRGVHCRLMLDSAGSVEFFRSPWFDIMKKSGIEIVEALKVSLIRIFLRRLDVRQHRKIILIDNYISYSGSM
NLVDPYLFKQSSGVGQWIDLMTRIEGPVATAIGIIYSCDWEIETGFKILPKLPNKEMLKKKCNQNSSIQVIASGPGFPKN
VIHQALLTAIYSARNELIMTTPYLVPSDDLLHAICTAAQRGVKVSIIIPLYHDSILVKWASRVFFSELLEAGVKIYQFKK
GLLHSKSILIDQQLSLIGTVNLDMRSLWLNFEITLVIDDRKFSHHLSAIQKEYIDNSRLLDKNTWSMRAYWTRILEKIFY
FLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=63.3744855967078, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=379, Percent_Identity=27.4406332453826, Blast_Score=125, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS_BUCAP (Q8K9P9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660609.1
- ProteinModelPortal:   Q8K9P9
- EnsemblBacteria:   EBBUCT00000000440
- GeneID:   1005464
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg262
- GeneTree:   EBGT00050000007948
- HOGENOM:   HBG694312
- OMA:   LIANITY
- ProtClustDB:   PRK01642
- BioCyc:   BAPH198804:BUSG262-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: NA

EC number: 2.7.8.-

Molecular weight: Translated: 56293; Mature: 56293

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 224-224 ACT_SITE 226-226 ACT_SITE 231-231 ACT_SITE 404-404 ACT_SITE 406-406 ACT_SITE 411-411

Signals:

None

Transmembrane regions:

HASH(0xc5e8fd4)-; HASH(0xc531930)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIFYDLIKWLVVLIYWLLIANITYRILIKRRSIPSSMSWLLTIYIIPFIGIAIWFFFGE
CCHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LYLGKRQKKIAKKIWSISNTYLNKLKSYKYIFQIKNSEVATSLFQLCKHRQGIYGIKNNK
HHHCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCE
ITLLTNTQNTIEVLIRDIYSARNNIEMVFYIWKPGGIADDVAMALIDSAKRGVHCRLMLD
EEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEC
SAGSVEFFRSPWFDIMKKSGIEIVEALKVSLIRIFLRRLDVRQHRKIILIDNYISYSGSM
CCCCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHCCEEEEEECEECCCCCC
NLVDPYLFKQSSGVGQWIDLMTRIEGPVATAIGIIYSCDWEIETGFKILPKLPNKEMLKK
CCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHEECCCEECCCCEECCCCCCHHHHHH
KCNQNSSIQVIASGPGFPKNVIHQALLTAIYSARNELIMTTPYLVPSDDLLHAICTAAQR
HCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
GVKVSIIIPLYHDSILVKWASRVFFSELLEAGVKIYQFKKGLLHSKSILIDQQLSLIGTV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHEHHHHHHEEEE
NLDMRSLWLNFEITLVIDDRKFSHHLSAIQKEYIDNSRLLDKNTWSMRAYWTRILEKIFY
CCCHHHEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHH
FLSPLL
HHCCCH
>Mature Secondary Structure
MDIFYDLIKWLVVLIYWLLIANITYRILIKRRSIPSSMSWLLTIYIIPFIGIAIWFFFGE
CCHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LYLGKRQKKIAKKIWSISNTYLNKLKSYKYIFQIKNSEVATSLFQLCKHRQGIYGIKNNK
HHHCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEECCCE
ITLLTNTQNTIEVLIRDIYSARNNIEMVFYIWKPGGIADDVAMALIDSAKRGVHCRLMLD
EEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEEC
SAGSVEFFRSPWFDIMKKSGIEIVEALKVSLIRIFLRRLDVRQHRKIILIDNYISYSGSM
CCCCCHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHCCEEEEEECEECCCCCC
NLVDPYLFKQSSGVGQWIDLMTRIEGPVATAIGIIYSCDWEIETGFKILPKLPNKEMLKK
CCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHEECCCEECCCCEECCCCCCHHHHHH
KCNQNSSIQVIASGPGFPKNVIHQALLTAIYSARNELIMTTPYLVPSDDLLHAICTAAQR
HCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
GVKVSIIIPLYHDSILVKWASRVFFSELLEAGVKIYQFKKGLLHSKSILIDQQLSLIGTV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHEHHHHHHEEEE
NLDMRSLWLNFEITLVIDDRKFSHHLSAIQKEYIDNSRLLDKNTWSMRAYWTRILEKIFY
CCCHHHEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHH
FLSPLL
HHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12089438