Definition Buchnera aphidicola str. Sg (Schizaphis graminum), complete genome.
Accession NC_004061
Length 641,454

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The map label for this gene is argS

Identifier: 21672518

GI number: 21672518

Start: 273397

End: 275139

Strand: Reverse

Name: argS

Synonym: BUsg237

Alternate gene names: 21672518

Gene position: 275139-273397 (Counterclockwise)

Preceding gene: 21672519

Following gene: 21672515

Centisome position: 42.89

GC content: 23.58

Gene sequence:

>1743_bases
ATGAATATAAAATATCTAATAAAAAAAGATATTGAACAAGCTTTAATCAAAATTAATAGTAATTACACTTATGAAGTATT
TATTATATCAAGTAAAAAAATAGAACTTGGTCATTATCAAGTTGACAATCTAATGAAAATATCTTCTAGATTGAAAATAA
AACCCTATAAATTATTTCAAAAAATACTGATTCTTATAGAAAAAAAAAAGATCTATAAAAAAATAATTTTTTCACATCCA
GGATTTATTAACATTTTTATTAAAAATGAATGGTTATCTGAAAAATTAGAAATACCATTTATTTCATCTCGTCTTGGTGT
CAAATATATTTATCCTAAAAAAAATATTGTAATAGATTATTCTTCTCCCAATATTGCAAAAGAAATGCATGTTGGACATT
TAAGATCAACTATAATAGGAGACGTTACAGTAAGAATACTAGAATTTTTAGGACAAAAGGTAATTAGAGCAAATCATATT
GGAGATTGGGGGACACAATTTGGTATACTCATTGCGTATTTAGAAGATAAAAAATTATTAAAAAAATTAAAAAAAAATCT
TCTTTCACTTCAAGATTTAGACAATATTTATTGTCAATCAAAAAAACAATACAATTCAAATGAACTATTTTCAAAAAAAT
CTAGAGAATTTGTAGTAAAATTACAAAATGGAGATCAATATTGTTGTGCTATTTGGAAAAAATTAGTATCAATAACTATG
TTAGAAAATTACAAAATATATAAAAAACTTAATGTAACATTGGAAGAAAAACATACTATGGGTGAAAGTTTCTATAACCC
AATGCTTCCGAAAATTGTAGAAGATCTTAAAAAACAAAAAATAGCTATTGAAAAAAATGGAGCTGTTATTGTTTTTCTTG
ATGAGTTTAAAAACAGGATGGGAGACTCCATGGGAGTTATAATCCGAAAAAAAGACAAAGGTTTTTTATATTCTACTACT
GATATTGCTTGCCTTAAATATAGGTATCAAAACTTACATGCTGATCGAATCATATATTATACTGATTCTCGTCAATATCA
ACATCTTCTACAATCATGGACTATTGCTGAAAAAGCTAATTATATATCTAAAAATTTATTATTAGAACATCATATGTTTG
GAATGATGTTATCTAAAAATAGACGTCCTTTTAAAACTCGTGACGGAAATACAATTAAACTTTCTAATCTACTTAATGAA
TCTATAAATAGAGCTACTAATTTAATTCAAAAAAAACAACCTAACTTATGCAAAAAAAAATTAATCAAATTAGGGGAAAT
AATTGGAATAAGTGCTGTAAAATATTCTGATCTATCTAAAAATAGAAACACTAATTATATATTTGATTGGGACAAAATGC
TAAGTTTTGAAGGTAATACAGCGCCTTATATACAATACGCTTATACAAGAATTATATCTATTTTAAAAAAGTCAAATATT
CCATTACATAAAATAAAAATGAAAATTATTCTTAATGAAGAAAGTGAAATTAACTTAGCTATTAAAATACTAGAATTTGA
AGAAATCATTCTATTAATAGCTAAAAATGGAACTCCTCATATTATGTGTAAATATCTTTATCAACTCTCTACGTATTTTT
CTCATTTTTATGAAAACTGTTCAATTCTCTTTTCTGAAAAAATAAAAATTCGAAAAAGCAGGATTAAATTATCTTTTTTA
ACAGCAAAAACAATAAAAAAAGGACTGAATTTATTAGGAATTAAAACAATAAAAAAAATGTAA

Upstream 100 bases:

>100_bases
AAAATAATATATTGTATTTAACGACATTTAAAAAAAATTTATAGCAAATCTTGTTTTCTAAAAATTTATTTTTTTAATTT
ATTAAAAAGGTTATTTATTT

Downstream 100 bases:

>100_bases
AAAAATAATTTATTTTTTTTAAAAAAATCAGTAAGGTGGGGAAATAACGCAGTACTAAAAACGTACTACCTAAAACCACA
CCAATCTGAATTTATAAAAT

Product: arginyl-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MNIKYLIKKDIEQALIKINSNYTYEVFIISSKKIELGHYQVDNLMKISSRLKIKPYKLFQKILILIEKKKIYKKIIFSHP
GFINIFIKNEWLSEKLEIPFISSRLGVKYIYPKKNIVIDYSSPNIAKEMHVGHLRSTIIGDVTVRILEFLGQKVIRANHI
GDWGTQFGILIAYLEDKKLLKKLKKNLLSLQDLDNIYCQSKKQYNSNELFSKKSREFVVKLQNGDQYCCAIWKKLVSITM
LENYKIYKKLNVTLEEKHTMGESFYNPMLPKIVEDLKKQKIAIEKNGAVIVFLDEFKNRMGDSMGVIIRKKDKGFLYSTT
DIACLKYRYQNLHADRIIYYTDSRQYQHLLQSWTIAEKANYISKNLLLEHHMFGMMLSKNRRPFKTRDGNTIKLSNLLNE
SINRATNLIQKKQPNLCKKKLIKLGEIIGISAVKYSDLSKNRNTNYIFDWDKMLSFEGNTAPYIQYAYTRIISILKKSNI
PLHKIKMKIILNEESEINLAIKILEFEEIILLIAKNGTPHIMCKYLYQLSTYFSHFYENCSILFSEKIKIRKSRIKLSFL
TAKTIKKGLNLLGIKTIKKM

Sequences:

>Translated_580_residues
MNIKYLIKKDIEQALIKINSNYTYEVFIISSKKIELGHYQVDNLMKISSRLKIKPYKLFQKILILIEKKKIYKKIIFSHP
GFINIFIKNEWLSEKLEIPFISSRLGVKYIYPKKNIVIDYSSPNIAKEMHVGHLRSTIIGDVTVRILEFLGQKVIRANHI
GDWGTQFGILIAYLEDKKLLKKLKKNLLSLQDLDNIYCQSKKQYNSNELFSKKSREFVVKLQNGDQYCCAIWKKLVSITM
LENYKIYKKLNVTLEEKHTMGESFYNPMLPKIVEDLKKQKIAIEKNGAVIVFLDEFKNRMGDSMGVIIRKKDKGFLYSTT
DIACLKYRYQNLHADRIIYYTDSRQYQHLLQSWTIAEKANYISKNLLLEHHMFGMMLSKNRRPFKTRDGNTIKLSNLLNE
SINRATNLIQKKQPNLCKKKLIKLGEIIGISAVKYSDLSKNRNTNYIFDWDKMLSFEGNTAPYIQYAYTRIISILKKSNI
PLHKIKMKIILNEESEINLAIKILEFEEIILLIAKNGTPHIMCKYLYQLSTYFSHFYENCSILFSEKIKIRKSRIKLSFL
TAKTIKKGLNLLGIKTIKKM
>Mature_580_residues
MNIKYLIKKDIEQALIKINSNYTYEVFIISSKKIELGHYQVDNLMKISSRLKIKPYKLFQKILILIEKKKIYKKIIFSHP
GFINIFIKNEWLSEKLEIPFISSRLGVKYIYPKKNIVIDYSSPNIAKEMHVGHLRSTIIGDVTVRILEFLGQKVIRANHI
GDWGTQFGILIAYLEDKKLLKKLKKNLLSLQDLDNIYCQSKKQYNSNELFSKKSREFVVKLQNGDQYCCAIWKKLVSITM
LENYKIYKKLNVTLEEKHTMGESFYNPMLPKIVEDLKKQKIAIEKNGAVIVFLDEFKNRMGDSMGVIIRKKDKGFLYSTT
DIACLKYRYQNLHADRIIYYTDSRQYQHLLQSWTIAEKANYISKNLLLEHHMFGMMLSKNRRPFKTRDGNTIKLSNLLNE
SINRATNLIQKKQPNLCKKKLIKLGEIIGISAVKYSDLSKNRNTNYIFDWDKMLSFEGNTAPYIQYAYTRIISILKKSNI
PLHKIKMKIILNEESEINLAIKILEFEEIILLIAKNGTPHIMCKYLYQLSTYFSHFYENCSILFSEKIKIRKSRIKLSFL
TAKTIKKGLNLLGIKTIKKM

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15149476, Length=573, Percent_Identity=35.0785340314136, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI197100773, Length=471, Percent_Identity=28.6624203821656, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1788184, Length=583, Percent_Identity=52.1440823327616, Blast_Score=676, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985061, Length=601, Percent_Identity=35.2745424292845, Blast_Score=334, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI71985068, Length=561, Percent_Identity=36.0071301247772, Blast_Score=324, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI17532153, Length=387, Percent_Identity=25.5813953488372, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320548, Length=514, Percent_Identity=29.7665369649805, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6321883, Length=547, Percent_Identity=27.0566727605119, Blast_Score=209, Evalue=8e-55,
Organism=Drosophila melanogaster, GI18859963, Length=572, Percent_Identity=35.1398601398601, Blast_Score=328, Evalue=5e-90,
Organism=Drosophila melanogaster, GI28571570, Length=482, Percent_Identity=26.3485477178423, Blast_Score=149, Evalue=7e-36,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYR_BUCAP (Q08888)

Other databases:

- EMBL:   AE013218
- EMBL:   L18927
- PIR:   I40591
- RefSeq:   NP_660585.1
- ProteinModelPortal:   Q08888
- SMR:   Q08888
- EnsemblBacteria:   EBBUCT00000000010
- GeneID:   1005438
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg237
- GeneTree:   EBGT00050000007717
- HOGENOM:   HBG695395
- OMA:   HMGFGTM
- ProtClustDB:   PRK01611
- BioCyc:   BAPH198804:BUSG237-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00123
- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.30.1360.70
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11956
- PRINTS:   PR01038
- SMART:   SM00836
- TIGRFAMs:   TIGR00456

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d; SSF55190 Arg-tRNA-synth_Ic_N; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.19

Molecular weight: Translated: 68044; Mature: 68044

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MNIKYLIKKDIEQALIKINSNYTYEVFIISSKKIELGHYQVDNLMKISSRLKIKPYKLFQ
CCCHHHHHHHHHHHHEEECCCCEEEEEEEECCEEEECCEEHHHHHHHHHCCCCCHHHHHH
KILILIEKKKIYKKIIFSHPGFINIFIKNEWLSEKLEIPFISSRLGVKYIYPKKNIVIDY
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCEEEEECCCCEEEEE
SSPNIAKEMHVGHLRSTIIGDVTVRILEFLGQKVIRANHIGDWGTQFGILIAYLEDKKLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHH
KKLKKNLLSLQDLDNIYCQSKKQYNSNELFSKKSREFVVKLQNGDQYCCAIWKKLVSITM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LENYKIYKKLNVTLEEKHTMGESFYNPMLPKIVEDLKKQKIAIEKNGAVIVFLDEFKNRM
HCCEEEEEEEECEEHHHHHHHHHHHCCHHHHHHHHHHHCEEEEECCCEEEEEHHHHHHHC
GDSMGVIIRKKDKGFLYSTTDIACLKYRYQNLHADRIIYYTDSRQYQHLLQSWTIAEKAN
CCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
YISKNLLLEHHMFGMMLSKNRRPFKTRDGNTIKLSNLLNESINRATNLIQKKQPNLCKKK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHH
LIKLGEIIGISAVKYSDLSKNRNTNYIFDWDKMLSFEGNTAPYIQYAYTRIISILKKSNI
HHHHHHHHEEEEEEECCCCCCCCCCEEEEHHHHEECCCCCCCHHHHHHHHHHHHHHHCCC
PLHKIKMKIILNEESEINLAIKILEFEEIILLIAKNGTPHIMCKYLYQLSTYFSHFYENC
CHHHEEEEEEECCCCCCEEEEEEEEHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SILFSEKIKIRKSRIKLSFLTAKTIKKGLNLLGIKTIKKM
EEEEHHHHHEEHHHEEEEEHHHHHHHCCCCEEEHHHHHCC
>Mature Secondary Structure
MNIKYLIKKDIEQALIKINSNYTYEVFIISSKKIELGHYQVDNLMKISSRLKIKPYKLFQ
CCCHHHHHHHHHHHHEEECCCCEEEEEEEECCEEEECCEEHHHHHHHHHCCCCCHHHHHH
KILILIEKKKIYKKIIFSHPGFINIFIKNEWLSEKLEIPFISSRLGVKYIYPKKNIVIDY
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCEEEEECCCCEEEEE
SSPNIAKEMHVGHLRSTIIGDVTVRILEFLGQKVIRANHIGDWGTQFGILIAYLEDKKLL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCHHHH
KKLKKNLLSLQDLDNIYCQSKKQYNSNELFSKKSREFVVKLQNGDQYCCAIWKKLVSITM
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LENYKIYKKLNVTLEEKHTMGESFYNPMLPKIVEDLKKQKIAIEKNGAVIVFLDEFKNRM
HCCEEEEEEEECEEHHHHHHHHHHHCCHHHHHHHHHHHCEEEEECCCEEEEEHHHHHHHC
GDSMGVIIRKKDKGFLYSTTDIACLKYRYQNLHADRIIYYTDSRQYQHLLQSWTIAEKAN
CCCEEEEEEECCCCEEEECCCHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
YISKNLLLEHHMFGMMLSKNRRPFKTRDGNTIKLSNLLNESINRATNLIQKKQPNLCKKK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHH
LIKLGEIIGISAVKYSDLSKNRNTNYIFDWDKMLSFEGNTAPYIQYAYTRIISILKKSNI
HHHHHHHHEEEEEEECCCCCCCCCCEEEEHHHHEECCCCCCCHHHHHHHHHHHHHHHCCC
PLHKIKMKIILNEESEINLAIKILEFEEIILLIAKNGTPHIMCKYLYQLSTYFSHFYENC
CHHHEEEEEEECCCCCCEEEEEEEEHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SILFSEKIKIRKSRIKLSFLTAKTIKKGLNLLGIKTIKKM
EEEEHHHHHEEHHHEEEEEHHHHHHHCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438; 7507875