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The map label for this gene is gloB

Identifier: 21672519

GI number: 21672519

Start: 277295

End: 278047

Strand: Reverse

Name: gloB

Synonym: BUsg238

Alternate gene names: 21672519

Gene position: 278047-277295 (Counterclockwise)

Preceding gene: 21672520

Following gene: 21672518

Centisome position: 43.35

GC content: 24.3

Gene sequence:

>753_bases
ATGATTTTAACAAGTATTTCTGTATTAAAAGATAATTACGTTTGGATTCTATACAACGATAATTGTTCTTGTATAATTAT
TGATCCTGGTGTTTCAGAAGATATAATAAAAAAAATAGAAAAAAAAAATTGGAAACTAATAGCAATTTTATTAACTCATA
ATCATATTGATCATGTAGGAGGAGTAGAAGAAATTATAAGAAGATATCCAAACGTAACTGTTTTTGGTCCTGAAGAAACA
AAAACAAGAAATGTAAATAAAATTGTAAAACAAGGAGATGTAATAAAATTGTTAAAAAGTGAAATTCATGTTTTTCTTAC
ACCAGGTCATACGTTAGGACATGTTTCATATTATTTAAAACCATATATTTTTTGCGGAGATACTTTATTTTCAGGAGGTT
GCGGTCGTGTTTTTAAAAATAAATTTTTTGATATGTATCAATCTATTAATTTTATAAAATCATTACCAAAAAAAACAATT
TTATGTTGTTCTCATGAATATACTCTATCTAATTTAAATTTCGCTATGTCTATTCTCCCATTTGATAAAAAAATAAAAAA
ATACTATAAGAAAATAAAAAAGCATATTTCTCAGAATAAGACAAGTCTTCCAGTTTCTCTTGAAACTGAAAAAAAAATAA
ATATTTTTTTAAGAACAAATGAGAAGACAATTAAAAAAGCAATGGGGTTAAAAAAAGACACGAGCAGCTTTGAGGTTTTT
ATTTTACTAAGGAAAGAGAAAGATGATTTTTAA

Upstream 100 bases:

>100_bases
CACGTCAATCTGCTCAAAGTCCTTCAGTTAAAGATTTTTTTTATGAAAATAACTTTTATCAAAACAAAAATTTATAAAAT
TTTTTTTAATGGAATTAAAC

Downstream 100 bases:

>100_bases
TTGGAGCTAAGCGGGATTGAACCGCTGACCTCCTGCGTGCAAGGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCCAGATA
ATGTTTACTATGGTAGGCCT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MILTSISVLKDNYVWILYNDNCSCIIIDPGVSEDIIKKIEKKNWKLIAILLTHNHIDHVGGVEEIIRRYPNVTVFGPEET
KTRNVNKIVKQGDVIKLLKSEIHVFLTPGHTLGHVSYYLKPYIFCGDTLFSGGCGRVFKNKFFDMYQSINFIKSLPKKTI
LCCSHEYTLSNLNFAMSILPFDKKIKKYYKKIKKHISQNKTSLPVSLETEKKINIFLRTNEKTIKKAMGLKKDTSSFEVF
ILLRKEKDDF

Sequences:

>Translated_250_residues
MILTSISVLKDNYVWILYNDNCSCIIIDPGVSEDIIKKIEKKNWKLIAILLTHNHIDHVGGVEEIIRRYPNVTVFGPEET
KTRNVNKIVKQGDVIKLLKSEIHVFLTPGHTLGHVSYYLKPYIFCGDTLFSGGCGRVFKNKFFDMYQSINFIKSLPKKTI
LCCSHEYTLSNLNFAMSILPFDKKIKKYYKKIKKHISQNKTSLPVSLETEKKINIFLRTNEKTIKKAMGLKKDTSSFEVF
ILLRKEKDDF
>Mature_250_residues
MILTSISVLKDNYVWILYNDNCSCIIIDPGVSEDIIKKIEKKNWKLIAILLTHNHIDHVGGVEEIIRRYPNVTVFGPEET
KTRNVNKIVKQGDVIKLLKSEIHVFLTPGHTLGHVSYYLKPYIFCGDTLFSGGCGRVFKNKFFDMYQSINFIKSLPKKTI
LCCSHEYTLSNLNFAMSILPFDKKIKKYYKKIKKHISQNKTSLPVSLETEKKINIFLRTNEKTIKKAMGLKKDTSSFEVF
ILLRKEKDDF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI14150041, Length=237, Percent_Identity=31.2236286919831, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI116642887, Length=257, Percent_Identity=31.1284046692607, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI21703352, Length=257, Percent_Identity=31.1284046692607, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI94538322, Length=259, Percent_Identity=28.957528957529, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI94538320, Length=259, Percent_Identity=28.957528957529, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI46361987, Length=189, Percent_Identity=32.2751322751323, Blast_Score=123, Evalue=1e-28,
Organism=Escherichia coli, GI1786406, Length=254, Percent_Identity=46.4566929133858, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1787158, Length=151, Percent_Identity=31.1258278145695, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536925, Length=232, Percent_Identity=31.8965517241379, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320478, Length=241, Percent_Identity=28.6307053941909, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324614, Length=259, Percent_Identity=26.6409266409266, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356335, Length=255, Percent_Identity=31.3725490196078, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24667703, Length=255, Percent_Identity=31.3725490196078, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24667711, Length=255, Percent_Identity=31.3725490196078, Blast_Score=144, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_BUCAP (Q08889)

Other databases:

- EMBL:   L18927
- EMBL:   AE013218
- PIR:   I40592
- RefSeq:   NP_660586.1
- ProteinModelPortal:   Q08889
- SMR:   Q08889
- EnsemblBacteria:   EBBUCT00000000200
- GeneID:   1005439
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg238
- GeneTree:   EBGT00050000008018
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK315822
- BioCyc:   BAPH198804:BUSG238-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28945; Mature: 28945

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILTSISVLKDNYVWILYNDNCSCIIIDPGVSEDIIKKIEKKNWKLIAILLTHNHIDHVG
CEEEEEEEECCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCHHCC
GVEEIIRRYPNVTVFGPEETKTRNVNKIVKQGDVIKLLKSEIHVFLTPGHTLGHVSYYLK
CHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCHHHHHHHCCEEEEECCCCHHHHHHHHHC
PYIFCGDTLFSGGCGRVFKNKFFDMYQSINFIKSLPKKTILCCSHEYTLSNLNFAMSILP
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCEEEEECC
FDKKIKKYYKKIKKHISQNKTSLPVSLETEKKINIFLRTNEKTIKKAMGLKKDTSSFEVF
CHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCHHHHHHHCCCCCCCCCEEEE
ILLRKEKDDF
EEEECCCCCC
>Mature Secondary Structure
MILTSISVLKDNYVWILYNDNCSCIIIDPGVSEDIIKKIEKKNWKLIAILLTHNHIDHVG
CEEEEEEEECCCEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCHHCC
GVEEIIRRYPNVTVFGPEETKTRNVNKIVKQGDVIKLLKSEIHVFLTPGHTLGHVSYYLK
CHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCHHHHHHHCCEEEEECCCCHHHHHHHHHC
PYIFCGDTLFSGGCGRVFKNKFFDMYQSINFIKSLPKKTILCCSHEYTLSNLNFAMSILP
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCEEEEECC
FDKKIKKYYKKIKKHISQNKTSLPVSLETEKKINIFLRTNEKTIKKAMGLKKDTSSFEVF
CHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCHHHHHHHCCCCCCCCCEEEE
ILLRKEKDDF
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7507875; 12089438