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The map label for this gene is proS

Identifier: 21672515

GI number: 21672515

Start: 268140

End: 269855

Strand: Reverse

Name: proS

Synonym: BUsg234

Alternate gene names: 21672515

Gene position: 269855-268140 (Counterclockwise)

Preceding gene: 21672518

Following gene: 21672505

Centisome position: 42.07

GC content: 24.71

Gene sequence:

>1716_bases
ATGCGTGCAAGTAAATATTTATTCTCAACTTTAAAAGAAACACCACATCATGCAAAAATTATCAGTCATCAACTTATGCT
AAAAAGTGGAATGATTAGAAAACTATCTTCAGGTATATATATTTGGCTTCCAACAGGTATCAGGGTATTAAATAAAATAA
AAGACATTATCAAAAATGAAATGAGAAAAATAAATGCTTTAGAAGTTTTAATGCCAGTTGTACAACCTAAAAAACTATGG
GAAAATAGTGGACGATTAAGTATATATGGAGAGGAATTATTTCAATTTTATGATCGACGTAATCAGAAATTTATATTAGG
ACCAACTAACGAAGAAGTCGTAACTAATTTTATTCGAAATGAAATTAATTCATTTAAAGAACTTCCTTTAATTATATATC
AAATACAAACAAAATTTCGAGATGAAATAAGGCCTAGATTTGGTATTGTTAGATCCCGCGAATTTATTATGAAAGATGCT
TATTCTTTCCATATTAATAAACATTGTTTGGAAAAAACTTATAATGATTTTTATCAAAGTTACATAAATATATTTAATAA
AATAAAAATTCAATTTCGTGCAGTAAATGCTGATTCAGGATCTATGGGAGGAAGTATTTCTCATGAATTTCAAGCTTTGT
CTGAAAATGGTGAAGATGAAATTGTTTTTTCAAAAAATACAGCATATGCTTCAAATATCAATACAGCAAAATCAATGCAA
AGTATTAACTTTTTAAAAGAAAAAAATCGAATAATTCCGAATCAAATAAAAAGTAAAAAATGCACAAAAAATTTTAATGA
ATTTAAAAAACCCATTAAAAATTTTATAAAAACTATTTTAGTTCGTACTAAAATTAATAATCAACCTTCCTTAGCTGCTC
TATTGATTCGTCAAGAACACGAATTAAATTTATTTAAAATAGAAGAAATCGATATAATTGAAAAACCATTATCATTTGTA
AATGAAGAAGAAATAATCACTTTAATGGGAGTTAAATCAAAATTTTTAGGCCCTTTAGGATTGAAAATTCCTATTTTCGC
TGACGTTTCTGTATATTATATGAAAGATTTTACTATTGGATCTAACATAAATGAAAAATTTTTTATTAATGTGAATTGGA
ATGTAGATCTTCCTATTCCAATTATTAAAGATATTAGAAATATAACAAAAAAAGATATAAGTTCAGATGGATCAAAATCT
TTAGAAATTAAGAAAAGCATTGAAATTGGACATATTTTTCAATTAGGAAAAGAATATTCAAAAAAAATGAACGTACTAGT
TCAAGAACAACACAATAACTATAAACATATACATATGGGGTGCTATGGTATAGGAATAACACGAATCGTTGCTTCTGTAA
TTGAACAAAATTATGATGATAATGGAATTATTTGGCCTGATTCTATCGCTCCCTTTCAAGTTGTAATCCTGCCTTTAAAT
ACAAAAAATTGTATTAAAATAAAAGAAATTACAGAAAATTTATACAAAATTTTAAAAAAAGAAAAAATAGACGTTATTTT
ATACGATCGCAATGAAAGACCAGGCATTATGTTTAATCAAATAGATTTAATTGGAATTCCACATCAAATTATAATTAGTC
CACGTCATATTAATGAAAATAAAGTTGAATATAGAGAAAGAAAAAATAACAAGAGTACCTTAATTAAGGTAAAAGAAATT
ACTTATTTTTTAAAAAAAAAATTAAACTTAATTTAA

Upstream 100 bases:

>100_bases
TATTAATCATAATTTAAAATAAACATTTTAAATATTTTTGAAATAAATATTTTATTTTAAAAAAAATTATTTTTATTCTT
ATAATCGAGACTTTTTTTAT

Downstream 100 bases:

>100_bases
AAGAACAAATCAAATTTTTTTAGAAATACTTTATTTGCATTTTGTTTTCTTTGGAAAAGATATCTAATTCAACTAAAAGT
TTATTACTAATTGTGATTAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRASKYLFSTLKETPHHAKIISHQLMLKSGMIRKLSSGIYIWLPTGIRVLNKIKDIIKNEMRKINALEVLMPVVQPKKLW
ENSGRLSIYGEELFQFYDRRNQKFILGPTNEEVVTNFIRNEINSFKELPLIIYQIQTKFRDEIRPRFGIVRSREFIMKDA
YSFHINKHCLEKTYNDFYQSYINIFNKIKIQFRAVNADSGSMGGSISHEFQALSENGEDEIVFSKNTAYASNINTAKSMQ
SINFLKEKNRIIPNQIKSKKCTKNFNEFKKPIKNFIKTILVRTKINNQPSLAALLIRQEHELNLFKIEEIDIIEKPLSFV
NEEEIITLMGVKSKFLGPLGLKIPIFADVSVYYMKDFTIGSNINEKFFINVNWNVDLPIPIIKDIRNITKKDISSDGSKS
LEIKKSIEIGHIFQLGKEYSKKMNVLVQEQHNNYKHIHMGCYGIGITRIVASVIEQNYDDNGIIWPDSIAPFQVVILPLN
TKNCIKIKEITENLYKILKKEKIDVILYDRNERPGIMFNQIDLIGIPHQIIISPRHINENKVEYRERKNNKSTLIKVKEI
TYFLKKKLNLI

Sequences:

>Translated_571_residues
MRASKYLFSTLKETPHHAKIISHQLMLKSGMIRKLSSGIYIWLPTGIRVLNKIKDIIKNEMRKINALEVLMPVVQPKKLW
ENSGRLSIYGEELFQFYDRRNQKFILGPTNEEVVTNFIRNEINSFKELPLIIYQIQTKFRDEIRPRFGIVRSREFIMKDA
YSFHINKHCLEKTYNDFYQSYINIFNKIKIQFRAVNADSGSMGGSISHEFQALSENGEDEIVFSKNTAYASNINTAKSMQ
SINFLKEKNRIIPNQIKSKKCTKNFNEFKKPIKNFIKTILVRTKINNQPSLAALLIRQEHELNLFKIEEIDIIEKPLSFV
NEEEIITLMGVKSKFLGPLGLKIPIFADVSVYYMKDFTIGSNINEKFFINVNWNVDLPIPIIKDIRNITKKDISSDGSKS
LEIKKSIEIGHIFQLGKEYSKKMNVLVQEQHNNYKHIHMGCYGIGITRIVASVIEQNYDDNGIIWPDSIAPFQVVILPLN
TKNCIKIKEITENLYKILKKEKIDVILYDRNERPGIMFNQIDLIGIPHQIIISPRHINENKVEYRERKNNKSTLIKVKEI
TYFLKKKLNLI
>Mature_571_residues
MRASKYLFSTLKETPHHAKIISHQLMLKSGMIRKLSSGIYIWLPTGIRVLNKIKDIIKNEMRKINALEVLMPVVQPKKLW
ENSGRLSIYGEELFQFYDRRNQKFILGPTNEEVVTNFIRNEINSFKELPLIIYQIQTKFRDEIRPRFGIVRSREFIMKDA
YSFHINKHCLEKTYNDFYQSYINIFNKIKIQFRAVNADSGSMGGSISHEFQALSENGEDEIVFSKNTAYASNINTAKSMQ
SINFLKEKNRIIPNQIKSKKCTKNFNEFKKPIKNFIKTILVRTKINNQPSLAALLIRQEHELNLFKIEEIDIIEKPLSFV
NEEEIITLMGVKSKFLGPLGLKIPIFADVSVYYMKDFTIGSNINEKFFINVNWNVDLPIPIIKDIRNITKKDISSDGSKS
LEIKKSIEIGHIFQLGKEYSKKMNVLVQEQHNNYKHIHMGCYGIGITRIVASVIEQNYDDNGIIWPDSIAPFQVVILPLN
TKNCIKIKEITENLYKILKKEKIDVILYDRNERPGIMFNQIDLIGIPHQIIISPRHINENKVEYRERKNNKSTLIKVKEI
TYFLKKKLNLI

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=215, Percent_Identity=39.5348837209302, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=48.4210526315789, Blast_Score=617, Evalue=1e-178,
Organism=Caenorhabditis elegans, GI115532348, Length=232, Percent_Identity=37.9310344827586, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=109, Percent_Identity=31.1926605504587, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=243, Percent_Identity=36.6255144032922, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322112, Length=171, Percent_Identity=30.4093567251462, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24656200, Length=222, Percent_Identity=33.7837837837838, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BUCAP (Q8K9S2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660582.1
- ProteinModelPortal:   Q8K9S2
- EnsemblBacteria:   EBBUCT00000000323
- GeneID:   1005434
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg234
- GeneTree:   EBGT00050000007910
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   BAPH198804:BUSG234-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 66613; Mature: 66613

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASKYLFSTLKETPHHAKIISHQLMLKSGMIRKLSSGIYIWLPTGIRVLNKIKDIIKNE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
MRKINALEVLMPVVQPKKLWENSGRLSIYGEELFQFYDRRNQKFILGPTNEEVVTNFIRN
HHHHHHHHHHHHHCCCHHHHCCCCCEEEEHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
EINSFKELPLIIYQIQTKFRDEIRPRFGIVRSREFIMKDAYSFHINKHCLEKTYNDFYQS
HHHHHHHCCEEEEHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YINIFNKIKIQFRAVNADSGSMGGSISHEFQALSENGEDEIVFSKNTAYASNINTAKSMQ
HHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEECCCCHHHHHH
SINFLKEKNRIIPNQIKSKKCTKNFNEFKKPIKNFIKTILVRTKINNQPSLAALLIRQEH
HHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEHHCC
ELNLFKIEEIDIIEKPLSFVNEEEIITLMGVKSKFLGPLGLKIPIFADVSVYYMKDFTIG
CCCEEEECHHHHHHHHHHHCCCCCEEEEEECHHHHCCCCCCEEEEEECCEEEEEEEEEEC
SNINEKFFINVNWNVDLPIPIIKDIRNITKKDISSDGSKSLEIKKSIEIGHIFQLGKEYS
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
KKMNVLVQEQHNNYKHIHMGCYGIGITRIVASVIEQNYDDNGIIWPDSIAPFQVVILPLN
HHHCEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEC
TKNCIKIKEITENLYKILKKEKIDVILYDRNERPGIMFNQIDLIGIPHQIIISPRHINEN
CCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECEEEEECCCEEEEECCCCCCC
KVEYRERKNNKSTLIKVKEITYFLKKKLNLI
HHHHHHHCCCCCCEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRASKYLFSTLKETPHHAKIISHQLMLKSGMIRKLSSGIYIWLPTGIRVLNKIKDIIKNE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
MRKINALEVLMPVVQPKKLWENSGRLSIYGEELFQFYDRRNQKFILGPTNEEVVTNFIRN
HHHHHHHHHHHHHCCCHHHHCCCCCEEEEHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
EINSFKELPLIIYQIQTKFRDEIRPRFGIVRSREFIMKDAYSFHINKHCLEKTYNDFYQS
HHHHHHHCCEEEEHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YINIFNKIKIQFRAVNADSGSMGGSISHEFQALSENGEDEIVFSKNTAYASNINTAKSMQ
HHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCEECCCCHHHHHH
SINFLKEKNRIIPNQIKSKKCTKNFNEFKKPIKNFIKTILVRTKINNQPSLAALLIRQEH
HHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEHHCC
ELNLFKIEEIDIIEKPLSFVNEEEIITLMGVKSKFLGPLGLKIPIFADVSVYYMKDFTIG
CCCEEEECHHHHHHHHHHHCCCCCEEEEEECHHHHCCCCCCEEEEEECCEEEEEEEEEEC
SNINEKFFINVNWNVDLPIPIIKDIRNITKKDISSDGSKSLEIKKSIEIGHIFQLGKEYS
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
KKMNVLVQEQHNNYKHIHMGCYGIGITRIVASVIEQNYDDNGIIWPDSIAPFQVVILPLN
HHHCEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEC
TKNCIKIKEITENLYKILKKEKIDVILYDRNERPGIMFNQIDLIGIPHQIIISPRHINEN
CCCCEEHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECEEEEECCCEEEEECCCCCCC
KVEYRERKNNKSTLIKVKEITYFLKKKLNLI
HHHHHHHCCCCCCEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438