Click here to switch to the map view.

The map label for this gene is map

Identifier: 21672505

GI number: 21672505

Start: 255297

End: 256082

Strand: Reverse

Name: map

Synonym: BUsg224

Alternate gene names: 21672505

Gene position: 256082-255297 (Counterclockwise)

Preceding gene: 21672515

Following gene: 21672504

Centisome position: 39.92

GC content: 27.1

Gene sequence:

>786_bases
ATGAATTGCATAATAAAAACAGAATCAGAAATTAAAAAAATGAAAGTATCTGGAAAAATAGCTGCAGAAGTATTAGAAAT
GATCGAAAACTATATAAAACCCAATATAAGTACCGAGGAAATTAATAATATTTGTCATAATTTCATTATTAAAAAAAAAG
CCGTTTCAGCATGTTTAGGATATCATGGTTTTCCAAAGTCTATTTGTATTTCTGTTAATGATGTAGTATGTCATGGAATC
CCAAATAAGAATCAAATTTTAAAATCAGGCGATATAGTTAATATCGATGTTACAATTATTAAAAAAAACTATCATGCAGA
TACATCAAAAATGTTTCTTGTAGGACAAACAAATATTTTATCTCAGCGTTTATGTAAAATAGCTCAAGAAAGTCTATACA
AATCTTTAAACATATTAAAACCAGGAATACCGTTATATAAAATAGGTGAAGTTATTCAAAATTACGTCGAAAATAACAAC
TTTTCAGTTGTAAAAGAATACTGTGGTCATGGCATTGGACGTGCATTTCACGAAGAACCATATGTATTACATTATAAAAA
TAAAAGTCATGTTATTTTAGAAAAAGGAATGATTTTTACCATTGAACCCATGATTAATGCAGGAAGTCATGAGGTTAAAT
GTATGAAAGATGGGTGGACTGTTAAAACAAAAGATCATTCTCTATCTGCACAATATGAACATACTGTATTAATAACAGAA
AACGGATGTGATATTTTAACATGGCAAAAAAATGAAAAAATTCCATCTAAGTTTATTAATAAATGA

Upstream 100 bases:

>100_bases
TTTATTTGACAATAAAATAAAAAATTATTTTCAAGTAAAATTTAAAACGTATAATTTAAATATTAAAAATTTTATTAAAT
ATAAAAATCGGCTAAATATT

Downstream 100 bases:

>100_bases
TTAATCTATTAAAAATTTAATAAAATATTAAATTAGGTCAATTTAAAAAATGAAAAAATATTATGACATTATCGAAAACG
CTTATTTAAAAAAAAATGAA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNCIIKTESEIKKMKVSGKIAAEVLEMIENYIKPNISTEEINNICHNFIIKKKAVSACLGYHGFPKSICISVNDVVCHGI
PNKNQILKSGDIVNIDVTIIKKNYHADTSKMFLVGQTNILSQRLCKIAQESLYKSLNILKPGIPLYKIGEVIQNYVENNN
FSVVKEYCGHGIGRAFHEEPYVLHYKNKSHVILEKGMIFTIEPMINAGSHEVKCMKDGWTVKTKDHSLSAQYEHTVLITE
NGCDILTWQKNEKIPSKFINK

Sequences:

>Translated_261_residues
MNCIIKTESEIKKMKVSGKIAAEVLEMIENYIKPNISTEEINNICHNFIIKKKAVSACLGYHGFPKSICISVNDVVCHGI
PNKNQILKSGDIVNIDVTIIKKNYHADTSKMFLVGQTNILSQRLCKIAQESLYKSLNILKPGIPLYKIGEVIQNYVENNN
FSVVKEYCGHGIGRAFHEEPYVLHYKNKSHVILEKGMIFTIEPMINAGSHEVKCMKDGWTVKTKDHSLSAQYEHTVLITE
NGCDILTWQKNEKIPSKFINK
>Mature_261_residues
MNCIIKTESEIKKMKVSGKIAAEVLEMIENYIKPNISTEEINNICHNFIIKKKAVSACLGYHGFPKSICISVNDVVCHGI
PNKNQILKSGDIVNIDVTIIKKNYHADTSKMFLVGQTNILSQRLCKIAQESLYKSLNILKPGIPLYKIGEVIQNYVENNN
FSVVKEYCGHGIGRAFHEEPYVLHYKNKSHVILEKGMIFTIEPMINAGSHEVKCMKDGWTVKTKDHSLSAQYEHTVLITE
NGCDILTWQKNEKIPSKFINK

Specific function: Removes the amino-terminal methionine from nascent proteins

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family

Homologues:

Organism=Homo sapiens, GI164420681, Length=250, Percent_Identity=41.2, Blast_Score=224, Evalue=6e-59,
Organism=Homo sapiens, GI40385867, Length=243, Percent_Identity=42.798353909465, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI1786364, Length=258, Percent_Identity=62.4031007751938, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71996291, Length=245, Percent_Identity=43.6734693877551, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323273, Length=257, Percent_Identity=42.4124513618677, Blast_Score=216, Evalue=2e-57,
Organism=Drosophila melanogaster, GI21355531, Length=255, Percent_Identity=39.6078431372549, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24583427, Length=246, Percent_Identity=39.0243902439024, Blast_Score=206, Evalue=1e-53,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): AMPM_BUCAP (Q8K9T1)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660572.1
- ProteinModelPortal:   Q8K9T1
- SMR:   Q8K9T1
- MEROPS:   M24.001
- EnsemblBacteria:   EBBUCT00000000027
- GeneID:   1005423
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg224
- GeneTree:   EBGT00050000008074
- HOGENOM:   HBG299384
- OMA:   GIPDDGK
- ProtClustDB:   CLSK315809
- BioCyc:   BAPH198804:BUSG224-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804:SF13
- PANTHER:   PTHR10804
- PRINTS:   PR00599
- TIGRFAMs:   TIGR00500

Pfam domain/function: PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: =3.4.11.18

Molecular weight: Translated: 29554; Mature: 29554

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00680 MAP_1

Important sites: BINDING 78-78 BINDING 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCIIKTESEIKKMKVSGKIAAEVLEMIENYIKPNISTEEINNICHNFIIKKKAVSACLG
CCEEEECHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
YHGFPKSICISVNDVVCHGIPNKNQILKSGDIVNIDVTIIKKNYHADTSKMFLVGQTNIL
CCCCCCCEEEEECCEEEECCCCCCCEECCCCEEEEEEEEEEECCCCCCCEEEEEECHHHH
SQRLCKIAQESLYKSLNILKPGIPLYKIGEVIQNYVENNNFSVVKEYCGHGIGRAFHEEP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHCHHHCCCC
YVLHYKNKSHVILEKGMIFTIEPMINAGSHEVKCMKDGWTVKTKDHSLSAQYEHTVLITE
EEEEECCCCEEEEECCEEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCEEEEEEEEC
NGCDILTWQKNEKIPSKFINK
CCCEEEEECCCCCCCHHHCCC
>Mature Secondary Structure
MNCIIKTESEIKKMKVSGKIAAEVLEMIENYIKPNISTEEINNICHNFIIKKKAVSACLG
CCEEEECHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
YHGFPKSICISVNDVVCHGIPNKNQILKSGDIVNIDVTIIKKNYHADTSKMFLVGQTNIL
CCCCCCCEEEEECCEEEECCCCCCCEECCCCEEEEEEEEEEECCCCCCCEEEEEECHHHH
SQRLCKIAQESLYKSLNILKPGIPLYKIGEVIQNYVENNNFSVVKEYCGHGIGRAFHEEP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHCHHHCCCC
YVLHYKNKSHVILEKGMIFTIEPMINAGSHEVKCMKDGWTVKTKDHSLSAQYEHTVLITE
EEEEECCCCEEEEECCEEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCEEEEEEEEC
NGCDILTWQKNEKIPSKFINK
CCCEEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438