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The map label for this gene is mraW

Identifier: 21672500

GI number: 21672500

Start: 248198

End: 249136

Strand: Reverse

Name: mraW

Synonym: BUsg218

Alternate gene names: 21672500

Gene position: 249136-248198 (Counterclockwise)

Preceding gene: 21672501

Following gene: 21672499

Centisome position: 38.84

GC content: 25.77

Gene sequence:

>939_bases
ATGAATCAAACAATCAAACATATTTCTGTAATGAAAAAAGAACTAATCGAATCATTAAAAATAAAAAAAAATGGCATTTA
TATTGATGGTACATTTGGAATGGGTGGTCATGCCCTTTCAATTTTAAAAACAATAGGAAGAGAAGGAAGACTCTATGCTA
TTGATAGAGATCCAAATTCTGTATTTATAGGCAATCAAATTAAAGACAAACGTTTTCATATTATTCATTCAAATTTTTCA
AAAATTTTAAATTATGCAAAATATAATAACATTATTGGCAAAGTTAACGGAATATTATTTGATCTTGGAACATCATCACT
GCAAATAGAAAATGAAAAAAGAGGCTTTTCATTTAAAAAAAATGGTCCTTTAGATATGAGAATGAATCCACATGCTGGTA
TTTCTGCTTCAGAGTGGCTTTTCAAAAGTGATATAAATAAAATTTATTTTGTTTTAAAAAATTTTGGGGAAGAGCGATTT
TCAAAAAGAATTGCTTATGCAATAAAAGACTATTGCAAAAAAAAGAAAATAAACAATACTTTTGAACTGGTAGATATTAT
AAAAAAAGCAACACCTATAAAAAACAAATTTAAACATCCTGCGAAAAGAACTTTTCAAGCTATTAGAATTTATATTAATC
AAGAACTAGAAGAAATTAAAAAAGGATTAGAAAATAGCTTAAAAATATTAAAGCCAGGAGGTCGTCTTTCTATTATTAGT
TTTCATTCTTTAGAAGATAGAATAGTAAAAAATTTTATGATGAAATATAGTAAAAAAGCTACTGTTCCATATGGTTTACC
AATTACAGAAAATAAATTAGAAACATTAAGAATATGTAAATTAAAAATTATTAATCGAATTTTTCCGACTCAAAAAGAAA
TTGAAAAAAATCCTCGAGCTCGCAGCTCTGTGCTTCGCACAGCTGAATTAAAAGAATAA

Upstream 100 bases:

>100_bases
ACAATATTTAAAAATAATTTATAAAAAATACATTAAACATAGTCATTTTCATTTGAAAATGACTATGTACTTTCTTTATT
TATATCATGTATATTCCAAT

Downstream 100 bases:

>100_bases
ATATGAAAATAAAACGTTATGATTTACCAAAAATAATTAAAAAAGATTTTTTTATATATGGAAAAATTCATTTAATTTTA
CTATTAGCAATAATTTTATC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNQTIKHISVMKKELIESLKIKKNGIYIDGTFGMGGHALSILKTIGREGRLYAIDRDPNSVFIGNQIKDKRFHIIHSNFS
KILNYAKYNNIIGKVNGILFDLGTSSLQIENEKRGFSFKKNGPLDMRMNPHAGISASEWLFKSDINKIYFVLKNFGEERF
SKRIAYAIKDYCKKKKINNTFELVDIIKKATPIKNKFKHPAKRTFQAIRIYINQELEEIKKGLENSLKILKPGGRLSIIS
FHSLEDRIVKNFMMKYSKKATVPYGLPITENKLETLRICKLKIINRIFPTQKEIEKNPRARSSVLRTAELKE

Sequences:

>Translated_312_residues
MNQTIKHISVMKKELIESLKIKKNGIYIDGTFGMGGHALSILKTIGREGRLYAIDRDPNSVFIGNQIKDKRFHIIHSNFS
KILNYAKYNNIIGKVNGILFDLGTSSLQIENEKRGFSFKKNGPLDMRMNPHAGISASEWLFKSDINKIYFVLKNFGEERF
SKRIAYAIKDYCKKKKINNTFELVDIIKKATPIKNKFKHPAKRTFQAIRIYINQELEEIKKGLENSLKILKPGGRLSIIS
FHSLEDRIVKNFMMKYSKKATVPYGLPITENKLETLRICKLKIINRIFPTQKEIEKNPRARSSVLRTAELKE
>Mature_312_residues
MNQTIKHISVMKKELIESLKIKKNGIYIDGTFGMGGHALSILKTIGREGRLYAIDRDPNSVFIGNQIKDKRFHIIHSNFS
KILNYAKYNNIIGKVNGILFDLGTSSLQIENEKRGFSFKKNGPLDMRMNPHAGISASEWLFKSDINKIYFVLKNFGEERF
SKRIAYAIKDYCKKKKINNTFELVDIIKKATPIKNKFKHPAKRTFQAIRIYINQELEEIKKGLENSLKILKPGGRLSIIS
FHSLEDRIVKNFMMKYSKKATVPYGLPITENKLETLRICKLKIINRIFPTQKEIEKNPRARSSVLRTAELKE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=34.2182890855457, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI165377202, Length=193, Percent_Identity=33.6787564766839, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1786270, Length=309, Percent_Identity=53.0744336569579, Blast_Score=357, Evalue=1e-100,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=35.1515151515151, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=35.1515151515151, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BUCAP (O85295)

Other databases:

- EMBL:   AF060492
- EMBL:   AE013218
- RefSeq:   NP_660567.1
- ProteinModelPortal:   O85295
- SMR:   O85295
- EnsemblBacteria:   EBBUCT00000000024
- GeneID:   1005417
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg218
- GeneTree:   EBGT00050000007757
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   BAPH198804:BUSG218-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36040; Mature: 36040

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 79-79 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTIKHISVMKKELIESLKIKKNGIYIDGTFGMGGHALSILKTIGREGRLYAIDRDPNS
CCCHHHHHHHHHHHHHHHHHECCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCE
VFIGNQIKDKRFHIIHSNFSKILNYAKYNNIIGKVNGILFDLGTSSLQIENEKRGFSFKK
EEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCC
NGPLDMRMNPHAGISASEWLFKSDINKIYFVLKNFGEERFSKRIAYAIKDYCKKKKINNT
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
FELVDIIKKATPIKNKFKHPAKRTFQAIRIYINQELEEIKKGLENSLKILKPGGRLSIIS
HHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
FHSLEDRIVKNFMMKYSKKATVPYGLPITENKLETLRICKLKIINRIFPTQKEIEKNPRA
EHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
RSSVLRTAELKE
HHHHHHHHHCCC
>Mature Secondary Structure
MNQTIKHISVMKKELIESLKIKKNGIYIDGTFGMGGHALSILKTIGREGRLYAIDRDPNS
CCCHHHHHHHHHHHHHHHHHECCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCE
VFIGNQIKDKRFHIIHSNFSKILNYAKYNNIIGKVNGILFDLGTSSLQIENEKRGFSFKK
EEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCC
NGPLDMRMNPHAGISASEWLFKSDINKIYFVLKNFGEERFSKRIAYAIKDYCKKKKINNT
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
FELVDIIKKATPIKNKFKHPAKRTFQAIRIYINQELEEIKKGLENSLKILKPGGRLSIIS
HHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEE
FHSLEDRIVKNFMMKYSKKATVPYGLPITENKLETLRICKLKIINRIFPTQKEIEKNPRA
EHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
RSSVLRTAELKE
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438