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The map label for this gene is ilvH

Identifier: 21672501

GI number: 21672501

Start: 249309

End: 249785

Strand: Reverse

Name: ilvH

Synonym: BUsg219

Alternate gene names: 21672501

Gene position: 249785-249309 (Counterclockwise)

Preceding gene: 21672502

Following gene: 21672500

Centisome position: 38.94

GC content: 30.19

Gene sequence:

>477_bases
ATGCGAAGAATTTTATCTGTTCTCTTAGAGAATGAATCAGGTGCATTATCACGAGTCATAGGATTGTTTTCACAGCGAGG
TTACAATATAGAAACAATTACAGTCGCACCAACAGAAGATCCTTCTCTATCAAAAATGACTATACAAACAATCGGAAATG
AAAAATCTATTGAGCAAATTGAAAAACAACTACACAAATTAATTGATGTATTAAGAGTAATAAAAGTCGGACAAAATTCT
CATATAGAACGTGAAATAATGTTATTAAAGGTACAAACAAACAACTGTAAAAAAGATGATGTTAAACATATTACCGAAGT
ATTTCGAGGACAAATTGTAGATATAACATCTACAACATATGTATTACAGATTACTGGTACTGCAAAAAAATTAGATTCTT
TTCTTAAAATAATTAGAAATACAACAGAAATCATTGAAATGACTCGTTCTGGGATAGTGGGAATTGCTCGAGGTTAA

Upstream 100 bases:

>100_bases
GATATTCAAATAGATGATTCTGAGCATGTTTATCCTATGCAAATTCAAGGTGGTGGCATGAATGAAATGTGGTTAAGGAA
AAAAGAGGTTTCCTAAAAAT

Downstream 100 bases:

>100_bases
CTTTTTTGTAAACATAGAAAATAACTTCTATTGAAAAAAATTCTTTTAAAAAAAAGATTTTTTATTTATATAACAATATT
TAAAAATAATTTATAAAAAA

Product: acetolactate synthase small subunit

Products: NA

Alternate protein names: Acetohydroxy-acid synthase small subunit; AHAS; ALS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRRILSVLLENESGALSRVIGLFSQRGYNIETITVAPTEDPSLSKMTIQTIGNEKSIEQIEKQLHKLIDVLRVIKVGQNS
HIEREIMLLKVQTNNCKKDDVKHITEVFRGQIVDITSTTYVLQITGTAKKLDSFLKIIRNTTEIIEMTRSGIVGIARG

Sequences:

>Translated_158_residues
MRRILSVLLENESGALSRVIGLFSQRGYNIETITVAPTEDPSLSKMTIQTIGNEKSIEQIEKQLHKLIDVLRVIKVGQNS
HIEREIMLLKVQTNNCKKDDVKHITEVFRGQIVDITSTTYVLQITGTAKKLDSFLKIIRNTTEIIEMTRSGIVGIARG
>Mature_158_residues
MRRILSVLLENESGALSRVIGLFSQRGYNIETITVAPTEDPSLSKMTIQTIGNEKSIEQIEKQLHKLIDVLRVIKVGQNS
HIEREIMLLKVQTNNCKKDDVKHITEVFRGQIVDITSTTYVLQITGTAKKLDSFLKIIRNTTEIIEMTRSGIVGIARG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0440

COG function: function code E; Acetolactate synthase, small (regulatory) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Escherichia coli, GI1786266, Length=158, Percent_Identity=67.0886075949367, Blast_Score=234, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319837, Length=205, Percent_Identity=24.8780487804878, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVH_BUCAP (O85294)

Other databases:

- EMBL:   AF060492
- EMBL:   AE013218
- RefSeq:   NP_660568.1
- ProteinModelPortal:   O85294
- SMR:   O85294
- EnsemblBacteria:   EBBUCT00000000121
- GeneID:   1005418
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg219
- GeneTree:   EBGT00050000007745
- HOGENOM:   HBG335310
- OMA:   IERELAM
- ProtClustDB:   CLSK315804
- BioCyc:   BAPH198804:BUSG219-MONOMER
- InterPro:   IPR004789
- InterPro:   IPR019455
- InterPro:   IPR002912
- TIGRFAMs:   TIGR00119

Pfam domain/function: PF01842 ACT; PF10369 ALS_ss_C

EC number: =2.2.1.6

Molecular weight: Translated: 17776; Mature: 17776

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRILSVLLENESGALSRVIGLFSQRGYNIETITVAPTEDPSLSKMTIQTIGNEKSIEQI
CHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHHHH
EKQLHKLIDVLRVIKVGQNSHIEREIMLLKVQTNNCKKDDVKHITEVFRGQIVDITSTTY
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCEE
VLQITGTAKKLDSFLKIIRNTTEIIEMTRSGIVGIARG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MRRILSVLLENESGALSRVIGLFSQRGYNIETITVAPTEDPSLSKMTIQTIGNEKSIEQI
CHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHHHH
EKQLHKLIDVLRVIKVGQNSHIEREIMLLKVQTNNCKKDDVKHITEVFRGQIVDITSTTY
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCEE
VLQITGTAKKLDSFLKIIRNTTEIIEMTRSGIVGIARG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9688822; 12089438