Click here to switch to the map view.

The map label for this gene is pfs

Identifier: 21672490

GI number: 21672490

Start: 232080

End: 232787

Strand: Reverse

Name: pfs

Synonym: BUsg204

Alternate gene names: 21672490

Gene position: 232787-232080 (Counterclockwise)

Preceding gene: 21672491

Following gene: 21672489

Centisome position: 36.29

GC content: 23.59

Gene sequence:

>708_bases
ATGAAAATTGGAATCATTGGTGCTATAGAACAAGAAATTAGAAAAATCAAAGAAATTATAAATAATTTAAAAATAAAAAA
AATTGGCAATATTAAAATTTATACAGGAACATTTAAAAAAATAGAAATTTTCTTGATTCTATCAGGAATTGGAAAAGTTT
CTGCTAGTATGTCTACTACGATTAGTATAAATTTATTTCAACCTGATTTTATTATTAATAGTGGTTCTGCGGGAAGTTTA
AATGCTTGTTTAAAGATAGGTGATATTATTATTCCCAAAAAAACATGTTACTATGATGTAGATTTAACAAATTTTGGCTA
TTCTAAGGGACAAATACCTGAATATCCTCAAACCTTTAAAACAAATAAAAATTTACGTGAAATTCTAAAAGAAATTGCTG
TTGAATTTAAATTTAAATTTTTGACAGGACTACTTGTTACTGGAGATTCTTTTATTAGAAAAAGTAATTGTATAAAAAAA
ATAAAAAATCAATTTTCTTCAGCAATAGGAGTAGATATGGAATCTACCGCAATAGGTCAAGTGTGTCATAATTTTAAAAT
ACCATTTATTATAATAAAATCAATATCTGATTTATCCGATAATAACGCTACTTCGCATTTTGAGAAAAACATTCCAATTG
CATCGTTAAAATCTTCAAAATTAGTTAAATTATTATTAAAAAAAATAAGTTCTCAAAATTCTATTTAA

Upstream 100 bases:

>100_bases
GCGGTCTATCTTTCAGTATTTAAAATATTGCATTAATTTTAAAAAATTAAAATTCTAAAAATTATTAATACTCTAAAAAA
ATTATATCTTATTGGAAAAA

Downstream 100 bases:

>100_bases
TTAAAAATAACATTTTTATTATATTCTTAATAAGTTTGTAGTATCTTTTGAATTCATATTCAAAAATGTATTATTTACAA
TAATCAATCGACTTTAAGAT

Product: 5'-methylthioadenosine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKIGIIGAIEQEIRKIKEIINNLKIKKIGNIKIYTGTFKKIEIFLILSGIGKVSASMSTTISINLFQPDFIINSGSAGSL
NACLKIGDIIIPKKTCYYDVDLTNFGYSKGQIPEYPQTFKTNKNLREILKEIAVEFKFKFLTGLLVTGDSFIRKSNCIKK
IKNQFSSAIGVDMESTAIGQVCHNFKIPFIIIKSISDLSDNNATSHFEKNIPIASLKSSKLVKLLLKKISSQNSI

Sequences:

>Translated_235_residues
MKIGIIGAIEQEIRKIKEIINNLKIKKIGNIKIYTGTFKKIEIFLILSGIGKVSASMSTTISINLFQPDFIINSGSAGSL
NACLKIGDIIIPKKTCYYDVDLTNFGYSKGQIPEYPQTFKTNKNLREILKEIAVEFKFKFLTGLLVTGDSFIRKSNCIKK
IKNQFSSAIGVDMESTAIGQVCHNFKIPFIIIKSISDLSDNNATSHFEKNIPIASLKSSKLVKLLLKKISSQNSI
>Mature_235_residues
MKIGIIGAIEQEIRKIKEIINNLKIKKIGNIKIYTGTFKKIEIFLILSGIGKVSASMSTTISINLFQPDFIINSGSAGSL
NACLKIGDIIIPKKTCYYDVDLTNFGYSKGQIPEYPQTFKTNKNLREILKEIAVEFKFKFLTGLLVTGDSFIRKSNCIKK
IKNQFSSAIGVDMESTAIGQVCHNFKIPFIIIKSISDLSDNNATSHFEKNIPIASLKSSKLVKLLLKKISSQNSI

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=230, Percent_Identity=40.8695652173913, Blast_Score=191, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_BUCAP (O51931)

Other databases:

- EMBL:   AF012886
- EMBL:   AE013218
- RefSeq:   NP_660557.1
- ProteinModelPortal:   O51931
- SMR:   O51931
- EnsemblBacteria:   EBBUCT00000000535
- GeneID:   1005401
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg204
- GeneTree:   EBGT00050000008047
- HOGENOM:   HBG367723
- OMA:   AMEQEVT
- ProtClustDB:   CLSK315792
- BioCyc:   BAPH198804:BUSG204-MONOMER
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 26103; Mature: 26103

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 152-152 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIGAIEQEIRKIKEIINNLKIKKIGNIKIYTGTFKKIEIFLILSGIGKVSASMSTT
CCEEEEHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCEEEEEEEEECCCCCCCCCEEE
ISINLFQPDFIINSGSAGSLNACLKIGDIIIPKKTCYYDVDLTNFGYSKGQIPEYPQTFK
EEEEEECCCEEEECCCCCCHHHHHEECCEEECCCCEEEEEEECCCCCCCCCCCCCCHHHC
TNKNLREILKEIAVEFKFKFLTGLLVTGDSFIRKSNCIKKIKNQFSSAIGVDMESTAIGQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VCHNFKIPFIIIKSISDLSDNNATSHFEKNIPIASLKSSKLVKLLLKKISSQNSI
HHHCCCCCEEEEECHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIGIIGAIEQEIRKIKEIINNLKIKKIGNIKIYTGTFKKIEIFLILSGIGKVSASMSTT
CCEEEEHHHHHHHHHHHHHHHCCCEEEECCEEEEECCCCEEEEEEEEECCCCCCCCCEEE
ISINLFQPDFIINSGSAGSLNACLKIGDIIIPKKTCYYDVDLTNFGYSKGQIPEYPQTFK
EEEEEECCCEEEECCCCCCHHHHHEECCEEECCCCEEEEEEECCCCCCCCCCCCCCHHHC
TNKNLREILKEIAVEFKFKFLTGLLVTGDSFIRKSNCIKKIKNQFSSAIGVDMESTAIGQ
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VCHNFKIPFIIIKSISDLSDNNATSHFEKNIPIASLKSSKLVKLLLKKISSQNSI
HHHCCCCCEEEEECHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9425245; 12089438