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The map label for this gene is speE

Identifier: 21672489

GI number: 21672489

Start: 230997

End: 231857

Strand: Reverse

Name: speE

Synonym: BUsg203

Alternate gene names: 21672489

Gene position: 231857-230997 (Counterclockwise)

Preceding gene: 21672490

Following gene: 21672488

Centisome position: 36.15

GC content: 24.62

Gene sequence:

>861_bases
ATGATTAACGAAAAAATATGGCATGAAAAACTTCATCGTCATGTTGGACAATATTTTTCAATTGATAAAATATTATATCA
AAAAAAAAATAAATACCATGATATTATCATTTTTCAAAATTCGATTATGGGACGAATTATGGCAATAGATGGTGTCGTTC
AAACAACAGAAAAAGATGAATTTATATATCATGAAATGTTAACTCATGTGCCTATATTTTATCACGGGTTGATAAAAAAT
GTACTAATAGTAGGTGGAGGAGACGGCGGTATACTACGAGAAATATGTCGACATAAGAGTATTGAAAATATCACTATGGT
TGAAATTGACTTAAATATTATTGATTTATGTAAACAATTTTTTCCCAAACATAACAATAATGCATATAAAGATCCACGTC
TAAAAATAGTTATAGATAATGGATTAAACTTCGTACAAAAAACAAAAGAAAAATTTGATCTTATTATATCAGATTCCACT
GATCCAATTGGTTGTGGAAAAGATTTATTTTTATCAGATTTTTACTTTCATTGTAAGAATTGTCTTGTTAAAAACGGTAT
ATTTGTAGGACAAAATGGCGTTTTTTTTCTTCAAAAAAATGATATTAATCAAAGCTATAAAAATTTAAAAAAAAACTTTC
ATGACGTAAGTTTTTATCAAGCAACTATTCCTAGTTATTATGGTGGAACAATGATGTTTTCTTGGGGTACTGATAATATA
GATTTTCGTCAAATAAATATTAAAAATTTAGAAAAACGTATAAAAGATAAAAAATTAACTTTTAATTACTATAATTCCAA
TATTCATATAAGCAGTTTTTATTTACCTCAATATATAATTAATACATTAGATAAAAGTTAA

Upstream 100 bases:

>100_bases
CCTTCTTTTAAAAAAGTCTAAAAATAAATTCTATTATTTCACTATTCAGTAGTGAACTATTTGTCCTCAAATTATTTATA
TTTTACAGGATAAAAAAACC

Downstream 100 bases:

>100_bases
AAATCCTGATATAGGAGAGTAATTAAATTGCAAAAACTAAAATTATATGGCTTTAATAATTTAACTAAAAGCCTAAGTTT
TTGTATTTATGATATTTGTT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MINEKIWHEKLHRHVGQYFSIDKILYQKKNKYHDIIIFQNSIMGRIMAIDGVVQTTEKDEFIYHEMLTHVPIFYHGLIKN
VLIVGGGDGGILREICRHKSIENITMVEIDLNIIDLCKQFFPKHNNNAYKDPRLKIVIDNGLNFVQKTKEKFDLIISDST
DPIGCGKDLFLSDFYFHCKNCLVKNGIFVGQNGVFFLQKNDINQSYKNLKKNFHDVSFYQATIPSYYGGTMMFSWGTDNI
DFRQINIKNLEKRIKDKKLTFNYYNSNIHISSFYLPQYIINTLDKS

Sequences:

>Translated_286_residues
MINEKIWHEKLHRHVGQYFSIDKILYQKKNKYHDIIIFQNSIMGRIMAIDGVVQTTEKDEFIYHEMLTHVPIFYHGLIKN
VLIVGGGDGGILREICRHKSIENITMVEIDLNIIDLCKQFFPKHNNNAYKDPRLKIVIDNGLNFVQKTKEKFDLIISDST
DPIGCGKDLFLSDFYFHCKNCLVKNGIFVGQNGVFFLQKNDINQSYKNLKKNFHDVSFYQATIPSYYGGTMMFSWGTDNI
DFRQINIKNLEKRIKDKKLTFNYYNSNIHISSFYLPQYIINTLDKS
>Mature_286_residues
MINEKIWHEKLHRHVGQYFSIDKILYQKKNKYHDIIIFQNSIMGRIMAIDGVVQTTEKDEFIYHEMLTHVPIFYHGLIKN
VLIVGGGDGGILREICRHKSIENITMVEIDLNIIDLCKQFFPKHNNNAYKDPRLKIVIDNGLNFVQKTKEKFDLIISDST
DPIGCGKDLFLSDFYFHCKNCLVKNGIFVGQNGVFFLQKNDINQSYKNLKKNFHDVSFYQATIPSYYGGTMMFSWGTDNI
DFRQINIKNLEKRIKDKKLTFNYYNSNIHISSFYLPQYIINTLDKS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=286, Percent_Identity=31.4685314685315, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1786312, Length=283, Percent_Identity=54.416961130742, Blast_Score=350, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI25154106, Length=271, Percent_Identity=36.5313653136531, Blast_Score=180, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI212645616, Length=218, Percent_Identity=38.5321100917431, Blast_Score=158, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323175, Length=272, Percent_Identity=36.7647058823529, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6325326, Length=268, Percent_Identity=32.8358208955224, Blast_Score=176, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24645443, Length=279, Percent_Identity=34.0501792114695, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI21355941, Length=216, Percent_Identity=34.2592592592593, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_BUCAP (Q8K9T5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660556.1
- ProteinModelPortal:   Q8K9T5
- SMR:   Q8K9T5
- EnsemblBacteria:   EBBUCT00000000020
- GeneID:   1005400
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg203
- GeneTree:   EBGT00050000008010
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- ProtClustDB:   PRK00811
- BioCyc:   BAPH198804:BUSG203-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 33548; Mature: 33548

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 33-33 BINDING 88-88 BINDING 108-108 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINEKIWHEKLHRHVGQYFSIDKILYQKKNKYHDIIIFQNSIMGRIMAIDGVVQTTEKDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCEEEEECCHHCCCCCHH
FIYHEMLTHVPIFYHGLIKNVLIVGGGDGGILREICRHKSIENITMVEIDLNIIDLCKQF
HHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHH
FPKHNNNAYKDPRLKIVIDNGLNFVQKTKEKFDLIISDSTDPIGCGKDLFLSDFYFHCKN
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHH
CLVKNGIFVGQNGVFFLQKNDINQSYKNLKKNFHDVSFYQATIPSYYGGTMMFSWGTDNI
HHHHCCEEECCCCEEEEEECCCCHHHHHHHHCCCCCHHHEECCCCCCCCEEEEEECCCCC
DFRQINIKNLEKRIKDKKLTFNYYNSNIHISSFYLPQYIINTLDKS
EEEEECHHHHHHHHCCCEEEEEEECCEEEEEEEEHHHHHHHHHCCC
>Mature Secondary Structure
MINEKIWHEKLHRHVGQYFSIDKILYQKKNKYHDIIIFQNSIMGRIMAIDGVVQTTEKDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHCEEEEECCHHCCCCCHH
FIYHEMLTHVPIFYHGLIKNVLIVGGGDGGILREICRHKSIENITMVEIDLNIIDLCKQF
HHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHH
FPKHNNNAYKDPRLKIVIDNGLNFVQKTKEKFDLIISDSTDPIGCGKDLFLSDFYFHCKN
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHH
CLVKNGIFVGQNGVFFLQKNDINQSYKNLKKNFHDVSFYQATIPSYYGGTMMFSWGTDNI
HHHHCCEEECCCCEEEEEECCCCHHHHHHHHCCCCCHHHEECCCCCCCCEEEEEECCCCC
DFRQINIKNLEKRIKDKKLTFNYYNSNIHISSFYLPQYIINTLDKS
EEEEECHHHHHHHHCCCEEEEEEECCEEEEEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438