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The map label for this gene is thrB

Identifier: 21672474

GI number: 21672474

Start: 209094

End: 210023

Strand: Reverse

Name: thrB

Synonym: BUsg187

Alternate gene names: 21672474

Gene position: 210023-209094 (Counterclockwise)

Preceding gene: 21672475

Following gene: 21672473

Centisome position: 32.74

GC content: 28.17

Gene sequence:

>930_bases
ATGATTAAAATTTATGCACCAGCTTCTGTTGCTAATGTTGGAGTTGGATTTGATATTTTAGGTGCAGCCATTATACCAAT
AAATGGTACTTTGTTAGGTGATTGTGTAACAGTAAAGTTATCAAAAAAATTTGAATTAATTAATAAAGGGATTTTTTCTA
ATAAATTACCTATAAATAAAGAACAAAACATTGTTTGGAAATGTTGGTTCAAATTTTGTAAAGTAATAAAAAAAAATATT
CCAGTTTCTATAATACTTGAAAAAAATATGCCCATCGGATCAGGACTAGGATCTAGTGCATGCTCAGTAGTTGCTACTTT
AGTTGCTATTAATCAAATTTGTAATAATCCTTTGAATTCAAAAGAATTACTATTACTTATGGGTGAAATAGAAGGTGAAA
TATCAGGAAGCATACATTATGATAATGTAGCACCATCTTATTTAGGAGGTCTTCAATTAATACTAGAAGATTCTGATATT
ATAAGTCAAAAGATCCCTAATTTTAAACATTGGTTTTGGATTATTGCTTGGCCAGGAATTAAAGTTTCTACTGCAGAAGC
AAGAAAAATTTTGCCTAAAGAATATAAAAAAGATATTTGTATTAAAAATAGTCGTTATTTAGCAGGTTTTATTCATGCAT
CGTATACTAAACAATCTCATTTAGCAGCTAGATGTATGCAAGATTTCATAGCAGAACCATATCGTAGTAAATTACTTCCT
AATTTTTTAAACGCCAAGGAAAAAATTAAAAAAATTGGAGCTATAAGTTGTGGAATATCTGGATCAGGTCCTACTATTTT
TGCAATTTCTGATAAAATAAAAACTGCTGAAAAGATATCTTTATGGCTAAAAAATAATTATTTACAAAATAAAACAGGAT
TTGTTCACATTTGTGCTGTAGATGTAAAAGGTGTGCGAAAAATAGGATAA

Upstream 100 bases:

>100_bases
CTTGTATTAAGAGGATATGGTGCGGGTAACGATGTTACTGCCTCTGGGGTGTTTTCCGACTTACTACGTATAATATTATA
AGCATCAGCGGAGTACAGTA

Downstream 100 bases:

>100_bases
AAAATGAAACTCTATAATTTAAAAAACCATAACGAACAAGTAAATTTTGAAACTGCCGTAAAACTTGGATTAGGACAGAA
ACAAGGTTTGTTTTTTCCAG

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MIKIYAPASVANVGVGFDILGAAIIPINGTLLGDCVTVKLSKKFELINKGIFSNKLPINKEQNIVWKCWFKFCKVIKKNI
PVSIILEKNMPIGSGLGSSACSVVATLVAINQICNNPLNSKELLLLMGEIEGEISGSIHYDNVAPSYLGGLQLILEDSDI
ISQKIPNFKHWFWIIAWPGIKVSTAEARKILPKEYKKDICIKNSRYLAGFIHASYTKQSHLAARCMQDFIAEPYRSKLLP
NFLNAKEKIKKIGAISCGISGSGPTIFAISDKIKTAEKISLWLKNNYLQNKTGFVHICAVDVKGVRKIG

Sequences:

>Translated_309_residues
MIKIYAPASVANVGVGFDILGAAIIPINGTLLGDCVTVKLSKKFELINKGIFSNKLPINKEQNIVWKCWFKFCKVIKKNI
PVSIILEKNMPIGSGLGSSACSVVATLVAINQICNNPLNSKELLLLMGEIEGEISGSIHYDNVAPSYLGGLQLILEDSDI
ISQKIPNFKHWFWIIAWPGIKVSTAEARKILPKEYKKDICIKNSRYLAGFIHASYTKQSHLAARCMQDFIAEPYRSKLLP
NFLNAKEKIKKIGAISCGISGSGPTIFAISDKIKTAEKISLWLKNNYLQNKTGFVHICAVDVKGVRKIG
>Mature_309_residues
MIKIYAPASVANVGVGFDILGAAIIPINGTLLGDCVTVKLSKKFELINKGIFSNKLPINKEQNIVWKCWFKFCKVIKKNI
PVSIILEKNMPIGSGLGSSACSVVATLVAINQICNNPLNSKELLLLMGEIEGEISGSIHYDNVAPSYLGGLQLILEDSDI
ISQKIPNFKHWFWIIAWPGIKVSTAEARKILPKEYKKDICIKNSRYLAGFIHASYTKQSHLAARCMQDFIAEPYRSKLLP
NFLNAKEKIKKIGAISCGISGSGPTIFAISDKIKTAEKISLWLKNNYLQNKTGFVHICAVDVKGVRKIG

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=306, Percent_Identity=60.1307189542484, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_BUCAP (Q8K9V0)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660541.1
- ProteinModelPortal:   Q8K9V0
- SMR:   Q8K9V0
- EnsemblBacteria:   EBBUCT00000000100
- GeneID:   1005384
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg187
- GeneTree:   EBGT00050000007703
- HOGENOM:   HBG646290
- OMA:   GSAHADN
- ProtClustDB:   PRK01212
- BioCyc:   BAPH198804:BUSG187-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 34037; Mature: 34037

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIYAPASVANVGVGFDILGAAIIPINGTLLGDCVTVKLSKKFELINKGIFSNKLPINK
CEEEECCCCHHHCCCCHHHHCEEEEECCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCC
EQNIVWKCWFKFCKVIKKNIPVSIILEKNMPIGSGLGSSACSVVATLVAINQICNNPLNS
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KELLLLMGEIEGEISGSIHYDNVAPSYLGGLQLILEDSDIISQKIPNFKHWFWIIAWPGI
CEEEEEEECCCCEECCEEEECCCCHHHHCCEEEEEECCHHHHHHCCCCCEEEEEEEECCE
KVSTAEARKILPKEYKKDICIKNSRYLAGFIHASYTKQSHLAARCMQDFIAEPYRSKLLP
EEEHHHHHHCCHHHHHCCEEECCCCEEEEEEEHHCCHHHHHHHHHHHHHHCCHHHHHHCH
NFLNAKEKIKKIGAISCGISGSGPTIFAISDKIKTAEKISLWLKNNYLQNKTGFVHICAV
HHHCHHHHHHHHCCEEECCCCCCCEEEEECCCCCCHHHHEEEEECCEECCCCCEEEEEEE
DVKGVRKIG
ECCCHHCCC
>Mature Secondary Structure
MIKIYAPASVANVGVGFDILGAAIIPINGTLLGDCVTVKLSKKFELINKGIFSNKLPINK
CEEEECCCCHHHCCCCHHHHCEEEEECCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCCC
EQNIVWKCWFKFCKVIKKNIPVSIILEKNMPIGSGLGSSACSVVATLVAINQICNNPLNS
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
KELLLLMGEIEGEISGSIHYDNVAPSYLGGLQLILEDSDIISQKIPNFKHWFWIIAWPGI
CEEEEEEECCCCEECCEEEECCCCHHHHCCEEEEEECCHHHHHHCCCCCEEEEEEEECCE
KVSTAEARKILPKEYKKDICIKNSRYLAGFIHASYTKQSHLAARCMQDFIAEPYRSKLLP
EEEHHHHHHCCHHHHHCCEEECCCCEEEEEEEHHCCHHHHHHHHHHHHHHCCHHHHHHCH
NFLNAKEKIKKIGAISCGISGSGPTIFAISDKIKTAEKISLWLKNNYLQNKTGFVHICAV
HHHCHHHHHHHHCCEEECCCCCCCEEEEECCCCCCHHHHEEEEECCEECCCCCEEEEEEE
DVKGVRKIG
ECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438