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The map label for this gene is thrA

Identifier: 21672475

GI number: 21672475

Start: 210043

End: 212487

Strand: Reverse

Name: thrA

Synonym: BUsg188

Alternate gene names: 21672475

Gene position: 212487-210043 (Counterclockwise)

Preceding gene: 21672477

Following gene: 21672474

Centisome position: 33.13

GC content: 25.52

Gene sequence:

>2445_bases
ATGAAACTATTGAAATTTGGTGGAACTTCGCTAGCTAATGCAAAAAAGTTTTTATGTGTAGCTGACATTATAGAAAAAAA
AAATAAAAAGGAACAAATTGCAGTTGTTCTTTCAGCTCCAGCTAAAATAACTAATTACTTAGCTACAATAATTGAAAATA
AGATTGATGATGAAGTTTTAAAAAAAATTAATCTTGCAAAAAACATTTTTATTGAATTAATACAAGACATTAAAAGAATA
CAACCACTTTTCCCATATGAAAATACAAAATCAACAATTGAAATAGAATTTAATAAATTAAAAAAAATAATCAATGGTAT
TCTTTTAATAAAACAATGTCCAGAAGGTATTAAACCTATCATAATATCTCGTGGAGAAATACTTTCAGTTGATATTATGA
AAAATATATTACAATCTAGAAATCATGAAGTTACTATTTTAAATCCAGTCACAAATCTTTTATCAATAGGAAACTATTTA
GATTCTACTATTGATATTAAAGAGTCTAAAAAACGTATTAAAAAAATTAATATAGATCAAAAAAATATTATTTTAATGGC
TGGTTTTATTGCAGGCAATAAAGAAGGAGAATTAGTAGTACTAGGACGTAATGGTTCTGATTATTCTGCTGCAATTTTAG
CTTCTTGCTTAAATGCCAAATGTTGTGAAATTTGGACTGATGTTGATGGTGTTTTGACAGCAGATCCGAGGATAGTTTCA
AATACTTACCTATTGGATTACATATCATATCAAGAAGCCATGGAATTATCTTATTTTGGGGCTAAGGTACTACATCCTCG
AACTATTGAACCAATTTCTCAGTTTCAAATACCTTGCGTCATAAAAAACACTAATAACACTGAGTCTAAGGGAACTTGGA
TTGGTAAAGAAAATAATCCAAGTGACAATTCTCTAAAAGGGGTGACATATCTTGATAACATCATAATGTTCAATATTTCT
GGTTCTTGTCTAAAAGATTCAGGAAATACAATTGCACGTATTTTTACTATTCTCTCAAGAGAGAGTATGAAAATTATTTT
AATAATACAATCTTCTTCAGAAAATCAAATAAATTTTTGTACTTTCGAAAAAGACATTGATTATATTTTATTAATATTAA
AAAAAGAATTTACGTTAGAAATAAAAGAAGGATTATTAAATGATTTTAATATAGTAAAAAATTTAACTATTTTGTCAGTT
ATTGGCTCTAATATTTCTGAAAAAAATAATATTGCATCAAAAATTTTTTCTTCTTTAGGTTCATCAAAAATCAATGTACT
TGCAATTGCACATGGATCTTCAAAACATTCTATATCAATAGTAATTAAAAAAGAAAACCTTTTACAAGGTATACAAAATA
TTCATAATACATTATTTTTTAAGAAAACAATCATAAATGTTTTTCTAATTGGAATAGGTGGAGTGGGAAAAGCATTATTA
AAACAAATCTTAAAGCAAGAAAAATTTTTAGACCAAAAAAATATAAAAATACAATTTCGTATGATTGCTAATTCTAAAAA
ATTACTTTTTTTAAAAAATTCAATAAATTTAAATAATTGGGAAGAAAATTTTAAAAAATCAAAAGAAAAATTTAATTTAA
CAATATTAAATGAACTATTAAAAAATACCTGCGATTCTAATTCAGTAATAATAGATTGTACATCTGATTATATTTTGTCA
AAGCAATATATCAGTTTCATTAAAAAGGGATTTCATATAATTACTTCGAATAAAAAAGCTAATACAGATTCGTTAAAATA
TTACAGTGAAATTAGAACTACTGCATTAAAAGAAAATAAAAAATTTTTATATGAAACTAATGTTGGAGCAGGATTACCAG
TTATTAACACACTTCAAAGTTTATTTAGTACAGGTGATTGTCTTATATCTTTTAAAGGTATATTATCTGGTTCTTTATCT
TTCATATTTGGAAAACTGGAAGAAGGTGTTTTATTATCTGAAGCAACTAAAGAAGCAAAAAAATTAGGTTTTACTGAACC
TAATCCCTTCGATGATTTATCTGGAATCGACGTTGCTAGAAAACTATTAGTTTTAGCTCGAGAAATTGGATATTCAATAG
AACTAAAAGACATAAGCATTGAACCCATATTACCAGAAAGATTTAAAAAATATCAAAATTCTGAAGAATTTTTATTTAAA
TTAAAAGAACTAGATTCATTCTTTTCAGAAAGAGTTAATAAAGCACGTGATATAGGCAATGTACTGCGTTTTATTGGAAG
TATAGAAAAAAATGGAAAATGTTCAGTGAAAATTGAGGAAATAAATAGCAATAATCCATTATATAAGGTAAAAAATGGTG
AAAATGCACTAACATTTTATACAAATTATTATCAACCAATTCCTCTTGTATTAAGAGGATATGGTGCGGGTAACGATGTT
ACTGCCTCTGGGGTGTTTTCCGACTTACTACGTATAATATTATAA

Upstream 100 bases:

>100_bases
ACTAATCATAATTAATATGAGACAATGTATATATTTAATAAAAACCCGCTCAAAAATAATGCGGGTTTTTTATGATATAA
ATTTAAGGATATAAAAAAAT

Downstream 100 bases:

>100_bases
GCATCAGCGGAGTACAGTAATGATTAAAATTTATGCACCAGCTTCTGTTGCTAATGTTGGAGTTGGATTTGATATTTTAG
GTGCAGCCATTATACCAATA

Product: bifunctional aspartokinase I/homeserine dehydrogenase I

Products: NA

Alternate protein names: AK-HD; Aspartokinase; Homoserine dehydrogenase

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MKLLKFGGTSLANAKKFLCVADIIEKKNKKEQIAVVLSAPAKITNYLATIIENKIDDEVLKKINLAKNIFIELIQDIKRI
QPLFPYENTKSTIEIEFNKLKKIINGILLIKQCPEGIKPIIISRGEILSVDIMKNILQSRNHEVTILNPVTNLLSIGNYL
DSTIDIKESKKRIKKINIDQKNIILMAGFIAGNKEGELVVLGRNGSDYSAAILASCLNAKCCEIWTDVDGVLTADPRIVS
NTYLLDYISYQEAMELSYFGAKVLHPRTIEPISQFQIPCVIKNTNNTESKGTWIGKENNPSDNSLKGVTYLDNIIMFNIS
GSCLKDSGNTIARIFTILSRESMKIILIIQSSSENQINFCTFEKDIDYILLILKKEFTLEIKEGLLNDFNIVKNLTILSV
IGSNISEKNNIASKIFSSLGSSKINVLAIAHGSSKHSISIVIKKENLLQGIQNIHNTLFFKKTIINVFLIGIGGVGKALL
KQILKQEKFLDQKNIKIQFRMIANSKKLLFLKNSINLNNWEENFKKSKEKFNLTILNELLKNTCDSNSVIIDCTSDYILS
KQYISFIKKGFHIITSNKKANTDSLKYYSEIRTTALKENKKFLYETNVGAGLPVINTLQSLFSTGDCLISFKGILSGSLS
FIFGKLEEGVLLSEATKEAKKLGFTEPNPFDDLSGIDVARKLLVLAREIGYSIELKDISIEPILPERFKKYQNSEEFLFK
LKELDSFFSERVNKARDIGNVLRFIGSIEKNGKCSVKIEEINSNNPLYKVKNGENALTFYTNYYQPIPLVLRGYGAGNDV
TASGVFSDLLRIIL

Sequences:

>Translated_814_residues
MKLLKFGGTSLANAKKFLCVADIIEKKNKKEQIAVVLSAPAKITNYLATIIENKIDDEVLKKINLAKNIFIELIQDIKRI
QPLFPYENTKSTIEIEFNKLKKIINGILLIKQCPEGIKPIIISRGEILSVDIMKNILQSRNHEVTILNPVTNLLSIGNYL
DSTIDIKESKKRIKKINIDQKNIILMAGFIAGNKEGELVVLGRNGSDYSAAILASCLNAKCCEIWTDVDGVLTADPRIVS
NTYLLDYISYQEAMELSYFGAKVLHPRTIEPISQFQIPCVIKNTNNTESKGTWIGKENNPSDNSLKGVTYLDNIIMFNIS
GSCLKDSGNTIARIFTILSRESMKIILIIQSSSENQINFCTFEKDIDYILLILKKEFTLEIKEGLLNDFNIVKNLTILSV
IGSNISEKNNIASKIFSSLGSSKINVLAIAHGSSKHSISIVIKKENLLQGIQNIHNTLFFKKTIINVFLIGIGGVGKALL
KQILKQEKFLDQKNIKIQFRMIANSKKLLFLKNSINLNNWEENFKKSKEKFNLTILNELLKNTCDSNSVIIDCTSDYILS
KQYISFIKKGFHIITSNKKANTDSLKYYSEIRTTALKENKKFLYETNVGAGLPVINTLQSLFSTGDCLISFKGILSGSLS
FIFGKLEEGVLLSEATKEAKKLGFTEPNPFDDLSGIDVARKLLVLAREIGYSIELKDISIEPILPERFKKYQNSEEFLFK
LKELDSFFSERVNKARDIGNVLRFIGSIEKNGKCSVKIEEINSNNPLYKVKNGENALTFYTNYYQPIPLVLRGYGAGNDV
TASGVFSDLLRIIL
>Mature_814_residues
MKLLKFGGTSLANAKKFLCVADIIEKKNKKEQIAVVLSAPAKITNYLATIIENKIDDEVLKKINLAKNIFIELIQDIKRI
QPLFPYENTKSTIEIEFNKLKKIINGILLIKQCPEGIKPIIISRGEILSVDIMKNILQSRNHEVTILNPVTNLLSIGNYL
DSTIDIKESKKRIKKINIDQKNIILMAGFIAGNKEGELVVLGRNGSDYSAAILASCLNAKCCEIWTDVDGVLTADPRIVS
NTYLLDYISYQEAMELSYFGAKVLHPRTIEPISQFQIPCVIKNTNNTESKGTWIGKENNPSDNSLKGVTYLDNIIMFNIS
GSCLKDSGNTIARIFTILSRESMKIILIIQSSSENQINFCTFEKDIDYILLILKKEFTLEIKEGLLNDFNIVKNLTILSV
IGSNISEKNNIASKIFSSLGSSKINVLAIAHGSSKHSISIVIKKENLLQGIQNIHNTLFFKKTIINVFLIGIGGVGKALL
KQILKQEKFLDQKNIKIQFRMIANSKKLLFLKNSINLNNWEENFKKSKEKFNLTILNELLKNTCDSNSVIIDCTSDYILS
KQYISFIKKGFHIITSNKKANTDSLKYYSEIRTTALKENKKFLYETNVGAGLPVINTLQSLFSTGDCLISFKGILSGSLS
FIFGKLEEGVLLSEATKEAKKLGFTEPNPFDDLSGIDVARKLLVLAREIGYSIELKDISIEPILPERFKKYQNSEEFLFK
LKELDSFFSERVNKARDIGNVLRFIGSIEKNGKCSVKIEEINSNNPLYKVKNGENALTFYTNYYQPIPLVLRGYGAGNDV
TASGVFSDLLRIIL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1786183, Length=814, Percent_Identity=52.3341523341523, Blast_Score=887, Evalue=0.0,
Organism=Escherichia coli, GI1790376, Length=823, Percent_Identity=27.460510328068, Blast_Score=332, Evalue=5e-92,
Organism=Escherichia coli, GI1790455, Length=463, Percent_Identity=27.4298056155508, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6322599, Length=333, Percent_Identity=32.1321321321321, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320893, Length=506, Percent_Identity=26.6798418972332, Blast_Score=144, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AKH_BUCAP (Q8K9U9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660542.1
- ProteinModelPortal:   Q8K9U9
- EnsemblBacteria:   EBBUCT00000000028
- GeneID:   1005385
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg188
- GeneTree:   EBGT00050000007710
- HOGENOM:   HBG298754
- OMA:   VKDGENA
- ProtClustDB:   PRK09436
- BioCyc:   BAPH198804:BUSG188-MONOMER
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.1160.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000727
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3; SSF53633 Aa_kinase

EC number: =2.7.2.4; =1.1.1.3

Molecular weight: Translated: 91380; Mature: 91380

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00324 ASPARTOKINASE; PS01042 HOMOSER_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKFGGTSLANAKKFLCVADIIEKKNKKEQIAVVLSAPAKITNYLATIIENKIDDEVL
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
KKINLAKNIFIELIQDIKRIQPLFPYENTKSTIEIEFNKLKKIINGILLIKQCPEGIKPI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEHHCCCCCCCE
IISRGEILSVDIMKNILQSRNHEVTILNPVTNLLSIGNYLDSTIDIKESKKRIKKINIDQ
EEECCCEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
KNIILMAGFIAGNKEGELVVLGRNGSDYSAAILASCLNAKCCEIWTDVDGVLTADPRIVS
CCEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHCCCCCEEECCCEEEC
NTYLLDYISYQEAMELSYFGAKVLHPRTIEPISQFQIPCVIKNTNNTESKGTWIGKENNP
CEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCCEEECCCCCC
SDNSLKGVTYLDNIIMFNISGSCLKDSGNTIARIFTILSRESMKIILIIQSSSENQINFC
CCCCCCCCEEECCEEEEECCCCEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCEEEE
TFEKDIDYILLILKKEFTLEIKEGLLNDFNIVKNLTILSVIGSNISEKNNIASKIFSSLG
EECCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
SSKINVLAIAHGSSKHSISIVIKKENLLQGIQNIHNTLFFKKTIINVFLIGIGGVGKALL
CCCEEEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHH
KQILKQEKFLDQKNIKIQFRMIANSKKLLFLKNSINLNNWEENFKKSKEKFNLTILNELL
HHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
KNTCDSNSVIIDCTSDYILSKQYISFIKKGFHIITSNKKANTDSLKYYSEIRTTALKENK
HCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCC
KFLYETNVGAGLPVINTLQSLFSTGDCLISFKGILSGSLSFIFGKLEEGVLLSEATKEAK
CEEEECCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHCCCEEHHHHHHHHH
KLGFTEPNPFDDLSGIDVARKLLVLAREIGYSIELKDISIEPILPERFKKYQNSEEFLFK
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHCCCHHHHHH
LKELDSFFSERVNKARDIGNVLRFIGSIEKNGKCSVKIEEINSNNPLYKVKNGENALTFY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEEE
TNYYQPIPLVLRGYGAGNDVTASGVFSDLLRIIL
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MKLLKFGGTSLANAKKFLCVADIIEKKNKKEQIAVVLSAPAKITNYLATIIENKIDDEVL
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCHHHH
KKINLAKNIFIELIQDIKRIQPLFPYENTKSTIEIEFNKLKKIINGILLIKQCPEGIKPI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHEEHHCCCCCCCE
IISRGEILSVDIMKNILQSRNHEVTILNPVTNLLSIGNYLDSTIDIKESKKRIKKINIDQ
EEECCCEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
KNIILMAGFIAGNKEGELVVLGRNGSDYSAAILASCLNAKCCEIWTDVDGVLTADPRIVS
CCEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHCCCCCEEECCCEEEC
NTYLLDYISYQEAMELSYFGAKVLHPRTIEPISQFQIPCVIKNTNNTESKGTWIGKENNP
CEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCCEEECCCCCC
SDNSLKGVTYLDNIIMFNISGSCLKDSGNTIARIFTILSRESMKIILIIQSSSENQINFC
CCCCCCCCEEECCEEEEECCCCEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCEEEE
TFEKDIDYILLILKKEFTLEIKEGLLNDFNIVKNLTILSVIGSNISEKNNIASKIFSSLG
EECCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
SSKINVLAIAHGSSKHSISIVIKKENLLQGIQNIHNTLFFKKTIINVFLIGIGGVGKALL
CCCEEEEEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHH
KQILKQEKFLDQKNIKIQFRMIANSKKLLFLKNSINLNNWEENFKKSKEKFNLTILNELL
HHHHHHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
KNTCDSNSVIIDCTSDYILSKQYISFIKKGFHIITSNKKANTDSLKYYSEIRTTALKENK
HCCCCCCCEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCC
KFLYETNVGAGLPVINTLQSLFSTGDCLISFKGILSGSLSFIFGKLEEGVLLSEATKEAK
CEEEECCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHCCCEEHHHHHHHHH
KLGFTEPNPFDDLSGIDVARKLLVLAREIGYSIELKDISIEPILPERFKKYQNSEEFLFK
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHCCCHHHHHH
LKELDSFFSERVNKARDIGNVLRFIGSIEKNGKCSVKIEEINSNNPLYKVKNGENALTFY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCEEEEE
TNYYQPIPLVLRGYGAGNDVTASGVFSDLLRIIL
ECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438