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The map label for this gene is rnt

Identifier: 21672469

GI number: 21672469

Start: 204291

End: 204950

Strand: Reverse

Name: rnt

Synonym: BUsg182

Alternate gene names: 21672469

Gene position: 204950-204291 (Counterclockwise)

Preceding gene: 21672473

Following gene: 21672465

Centisome position: 31.95

GC content: 31.52

Gene sequence:

>660_bases
ATGTCTATAACTCAAGAATTCAATTTGTTAAGTAATCGATTTCGCACTTTTTATCCTGTTGTTATCGACATTGAAACAGC
AGGGTTTAATCCAAAAACTGATGCAGTATTAGAAATCGCATTAATAACTTTAAAAATGGATAAATTAGGATGGCTGCATA
AAGAAGATATACTTCACTTTCATATAAAACCATTTAAGGGATCTATTATCAATTCTGATGCTATTGCTTTTAATAAAATT
GATCCATTTAATCCTTTACGTGGAGCTATTAGTGAAACATTAGCTATTGAATCAATATTAGAAAAAGTAAATTATGGAAT
AAAAATACAGGGATGCACTCGGGGGATAGTCGTAGCACATAATGCTCATTTTGATCATAATTTTTTAATGGCAGCTATTC
AAAGAGTTAAAATTAAAAACAATCCCTTTCATCCCTTTGTTACATTTGATACAGCCGCATTGAGTGGGCTAGCCGTAGGT
CAAACTGTGTTAGCAAAAGCATGCAAGGCAATTGGTTTGTCATTTGACAATAATCAAGCGCATTCAGCACTATACGATAG
TTTACAAACAGCTAATCTTTTTTGTAAAATAGTAAATCGTTGGAAAGGTTTAGGCGGATGGCCTATAAATGTTAAAAAAA
GTAAACAAAATTCATATTAA

Upstream 100 bases:

>100_bases
TCTATCAATAAAACCAAAAAAATATAACTTAAAAGATTAATAAAAATAAATCAAAAGTTAACTATATTACACACGAAGTG
TTTTAAATGATGAGATTTTG

Downstream 100 bases:

>100_bases
GTTAATAATAAAAAAAATATTTTTAAACTTTTTATAAAAAAAGATATGACATTAATAATAACAATTCTGATTTTTACATA
ATGTCATATCTTATTTTGTT

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSITQEFNLLSNRFRTFYPVVIDIETAGFNPKTDAVLEIALITLKMDKLGWLHKEDILHFHIKPFKGSIINSDAIAFNKI
DPFNPLRGAISETLAIESILEKVNYGIKIQGCTRGIVVAHNAHFDHNFLMAAIQRVKIKNNPFHPFVTFDTAALSGLAVG
QTVLAKACKAIGLSFDNNQAHSALYDSLQTANLFCKIVNRWKGLGGWPINVKKSKQNSY

Sequences:

>Translated_219_residues
MSITQEFNLLSNRFRTFYPVVIDIETAGFNPKTDAVLEIALITLKMDKLGWLHKEDILHFHIKPFKGSIINSDAIAFNKI
DPFNPLRGAISETLAIESILEKVNYGIKIQGCTRGIVVAHNAHFDHNFLMAAIQRVKIKNNPFHPFVTFDTAALSGLAVG
QTVLAKACKAIGLSFDNNQAHSALYDSLQTANLFCKIVNRWKGLGGWPINVKKSKQNSY
>Mature_218_residues
SITQEFNLLSNRFRTFYPVVIDIETAGFNPKTDAVLEIALITLKMDKLGWLHKEDILHFHIKPFKGSIINSDAIAFNKID
PFNPLRGAISETLAIESILEKVNYGIKIQGCTRGIVVAHNAHFDHNFLMAAIQRVKIKNNPFHPFVTFDTAALSGLAVGQ
TVLAKACKAIGLSFDNNQAHSALYDSLQTANLFCKIVNRWKGLGGWPINVKKSKQNSY

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Escherichia coli, GI1787941, Length=214, Percent_Identity=61.214953271028, Blast_Score=288, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNT_BUCAP (Q8K9V5)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660536.1
- ProteinModelPortal:   Q8K9V5
- SMR:   Q8K9V5
- EnsemblBacteria:   EBBUCT00000000413
- GeneID:   1005379
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg182
- GeneTree:   EBGT00050000008097
- HOGENOM:   HBG289562
- OMA:   YDAEKTA
- ProtClustDB:   PRK05168
- BioCyc:   BAPH198804:BUSG182-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00157
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR01298

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: 3.1.13.-

Molecular weight: Translated: 24413; Mature: 24281

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITQEFNLLSNRFRTFYPVVIDIETAGFNPKTDAVLEIALITLKMDKLGWLHKEDILHF
CCCCHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHEEEEHEEEEHHHHCCCCCCCEEEE
HIKPFKGSIINSDAIAFNKIDPFNPLRGAISETLAIESILEKVNYGIKIQGCTRGIVVAH
EEECCCCCEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
NAHFDHNFLMAAIQRVKIKNNPFHPFVTFDTAALSGLAVGQTVLAKACKAIGLSFDNNQA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
HSALYDSLQTANLFCKIVNRWKGLGGWPINVKKSKQNSY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SITQEFNLLSNRFRTFYPVVIDIETAGFNPKTDAVLEIALITLKMDKLGWLHKEDILHF
CCCHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHEEEEHEEEEHHHHCCCCCCCEEEE
HIKPFKGSIINSDAIAFNKIDPFNPLRGAISETLAIESILEKVNYGIKIQGCTRGIVVAH
EEECCCCCEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEE
NAHFDHNFLMAAIQRVKIKNNPFHPFVTFDTAALSGLAVGQTVLAKACKAIGLSFDNNQA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
HSALYDSLQTANLFCKIVNRWKGLGGWPINVKKSKQNSY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438