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The map label for this gene is grpE

Identifier: 21672465

GI number: 21672465

Start: 201376

End: 201942

Strand: Reverse

Name: grpE

Synonym: BUsg178

Alternate gene names: 21672465

Gene position: 201942-201376 (Counterclockwise)

Preceding gene: 21672469

Following gene: 21672464

Centisome position: 31.48

GC content: 19.4

Gene sequence:

>567_bases
ATGGATAATCAAGAGAAAAAAACAAACTATCAAAATACTGATAAAGAAAATGATTTAGAAAAAAATAAAGAAAAAAAAAA
TGATGAATCAATATTTCAAAATAAAAAAATGAATGAAATAAGGGAAAAAATATTCAAAAATAAAAAAGAAATAAATAATC
TTAAACTAAGACATCTAGCAAATATAGAAAATATTAAAAAAAATACTGAAAAAAAAATAAAAAAAATAAAAAATGCAGAA
ATAGAAAACTTTTTTAAACAAATTATTCCTATTATAAATAATCTTGAAGACATATTAACAATTTCTACAAAGTTAAATTT
AAATGATGAACCATCAATACAGGGGATAGAATTAACATTAAAATCTTTGCTAAGTATATTAATAAAATTTGGAGTAAAAA
TAGAAGGCAAAAAAAATGAAATATTCAATCCAAAAATTCATGATGTCATCTTAACTGAGTCATCAAATACAATAGAACCA
AATTATATTATTTCTGTAAAGAAAAAAGGTTTTATTTTTAAAAAAACAATACTTCGTAAGGCAGCTGTTGTAATTTCTAA
AACTTAA

Upstream 100 bases:

>100_bases
TAAATTTCCCAATAAAAAAAATATTTTTAATAAAAAAATTACCTTGAAAAACATATTAAAAACTCTACATTTAATTAATA
ACATTTAAAATGGTAATATT

Downstream 100 bases:

>100_bases
AAAAATATATTTTGATATTAATAAAAAACATTTTTAAAATTTTTTAAAAATAGAAAAAATTTAAAATTTTTTCTATTTTT
AAATCTTAAACCAATCAATT

Product: hypothetical protein

Products: NA

Alternate protein names: HSP-70 cofactor 2

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MDNQEKKTNYQNTDKENDLEKNKEKKNDESIFQNKKMNEIREKIFKNKKEINNLKLRHLANIENIKKNTEKKIKKIKNAE
IENFFKQIIPIINNLEDILTISTKLNLNDEPSIQGIELTLKSLLSILIKFGVKIEGKKNEIFNPKIHDVILTESSNTIEP
NYIISVKKKGFIFKKTILRKAAVVISKT

Sequences:

>Translated_188_residues
MDNQEKKTNYQNTDKENDLEKNKEKKNDESIFQNKKMNEIREKIFKNKKEINNLKLRHLANIENIKKNTEKKIKKIKNAE
IENFFKQIIPIINNLEDILTISTKLNLNDEPSIQGIELTLKSLLSILIKFGVKIEGKKNEIFNPKIHDVILTESSNTIEP
NYIISVKKKGFIFKKTILRKAAVVISKT
>Mature_188_residues
MDNQEKKTNYQNTDKENDLEKNKEKKNDESIFQNKKMNEIREKIFKNKKEINNLKLRHLANIENIKKNTEKKIKKIKNAE
IENFFKQIIPIINNLEDILTISTKLNLNDEPSIQGIELTLKSLLSILIKFGVKIEGKKNEIFNPKIHDVILTESSNTIEP
NYIISVKKKGFIFKKTILRKAAVVISKT

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=133, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE2_BUCAP (Q8K9V9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660532.1
- ProteinModelPortal:   Q8K9V9
- SMR:   Q8K9V9
- EnsemblBacteria:   EBBUCT00000000197
- GeneID:   1005375
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg178
- GeneTree:   EBGT00050000007962
- HOGENOM:   HBG038982
- OMA:   LMVANDE
- ProtClustDB:   CLSK315751
- BioCyc:   BAPH198804:BUSG178-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21950; Mature: 21950

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNQEKKTNYQNTDKENDLEKNKEKKNDESIFQNKKMNEIREKIFKNKKEINNLKLRHLA
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
NIENIKKNTEKKIKKIKNAEIENFFKQIIPIINNLEDILTISTKLNLNDEPSIQGIELTL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHEEHHEECCCCCCCCCHHHHHH
KSLLSILIKFGVKIEGKKNEIFNPKIHDVILTESSNTIEPNYIISVKKKGFIFKKTILRK
HHHHHHHHHHCCEECCCCCCCCCCCHHEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHH
AAVVISKT
HHHHHCCC
>Mature Secondary Structure
MDNQEKKTNYQNTDKENDLEKNKEKKNDESIFQNKKMNEIREKIFKNKKEINNLKLRHLA
CCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
NIENIKKNTEKKIKKIKNAEIENFFKQIIPIINNLEDILTISTKLNLNDEPSIQGIELTL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHEEHHEECCCCCCCCCHHHHHH
KSLLSILIKFGVKIEGKKNEIFNPKIHDVILTESSNTIEPNYIISVKKKGFIFKKTILRK
HHHHHHHHHHCCEECCCCCCCCCCCHHEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHH
AAVVISKT
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438