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The map label for this gene is nadE

Identifier: 21672457

GI number: 21672457

Start: 188190

End: 188996

Strand: Reverse

Name: nadE

Synonym: BUsg168

Alternate gene names: 21672457

Gene position: 188996-188190 (Counterclockwise)

Preceding gene: 21672460

Following gene: 21672453

Centisome position: 29.46

GC content: 26.52

Gene sequence:

>807_bases
ATGACATTACAAAAGAAAATTATCGAATTATTAGGAGTTAAACCGCTAATTATACCTAAAAAAGAAATAGAAAATCGTAT
TCAGATATTAAAAAGATACTTAATTGAAAATACCCATTTAAAAACGTTAATAGTAGGTATCAGCGGGGGACAAGATTCAA
CTCTAACTGGAAAATTATGTCAGTTATCAATTCAAGAGTTACGAAAAGAAAAAAAAGAAAAATCTTATCAATTTATCGCA
TTAAGATTACCTTATGGTGTACAAATAGACGAAAAAGATTGCAGAGATGCAATTAATTTTATAAATCCAGATCAAATTTT
TACAATTAATATTAAAAACGCAGTTTTAAATAGTGAAAGATCATTAAAAAAACAAGGAATACAAATCTCAGACTATATCA
AAGGCAATGAAAAAGCAAGAGAAAGAATGAAAGTACAGTATAGTTTTGCAGCTATAACAAATGGACTCGTTGTTGGAACA
GGAAACGCAGCAGAAAACGTCACTGGATTTTTTACAAAATATGGAGACAACGGAACAGATGTTAACTTAATTTCTAAATT
AAATAAAAGACAAGGTAAATTTCTATTAAAAGAATTAAACTGTCCTAAACACTTATATTTAAAAAAACCTACTGCTGATC
TTGAAGATGAGAAACCACAAAAAGAAGACGAAGTAGCATTAGGTATAAAATATAATATAATTGATGATTATTTGGAAGGA
AAAAAAGTAAATTCTTTAAATAAACAAATAATTGAAAGGTTATATTTAACAACAGAACATAAACGTAAAATAATTAACTT
AGGATAA

Upstream 100 bases:

>100_bases
TTTATATATAAACTTTTGCAATTATTATTTTTAATATATAATCATGAACTCTATGTATAAAAGATAATGTTTAAAATAAA
ACTATAATAACAGGACAAAA

Downstream 100 bases:

>100_bases
AATATTTAAAAAAAATTACTAATGCAATATCATTTTTTTCTGCTCAATAGCATGAATTTGTACTTTAATGATTTCACTAA
TAGGATCTTCAGGACAATGT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTLQKKIIELLGVKPLIIPKKEIENRIQILKRYLIENTHLKTLIVGISGGQDSTLTGKLCQLSIQELRKEKKEKSYQFIA
LRLPYGVQIDEKDCRDAINFINPDQIFTINIKNAVLNSERSLKKQGIQISDYIKGNEKARERMKVQYSFAAITNGLVVGT
GNAAENVTGFFTKYGDNGTDVNLISKLNKRQGKFLLKELNCPKHLYLKKPTADLEDEKPQKEDEVALGIKYNIIDDYLEG
KKVNSLNKQIIERLYLTTEHKRKIINLG

Sequences:

>Translated_268_residues
MTLQKKIIELLGVKPLIIPKKEIENRIQILKRYLIENTHLKTLIVGISGGQDSTLTGKLCQLSIQELRKEKKEKSYQFIA
LRLPYGVQIDEKDCRDAINFINPDQIFTINIKNAVLNSERSLKKQGIQISDYIKGNEKARERMKVQYSFAAITNGLVVGT
GNAAENVTGFFTKYGDNGTDVNLISKLNKRQGKFLLKELNCPKHLYLKKPTADLEDEKPQKEDEVALGIKYNIIDDYLEG
KKVNSLNKQIIERLYLTTEHKRKIINLG
>Mature_267_residues
TLQKKIIELLGVKPLIIPKKEIENRIQILKRYLIENTHLKTLIVGISGGQDSTLTGKLCQLSIQELRKEKKEKSYQFIAL
RLPYGVQIDEKDCRDAINFINPDQIFTINIKNAVLNSERSLKKQGIQISDYIKGNEKARERMKVQYSFAAITNGLVVGTG
NAAENVTGFFTKYGDNGTDVNLISKLNKRQGKFLLKELNCPKHLYLKKPTADLEDEKPQKEDEVALGIKYNIIDDYLEGK
KVNSLNKQIIERLYLTTEHKRKIINLG

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=263, Percent_Identity=61.2167300380228, Blast_Score=341, Evalue=3e-95,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_BUCAP (Q8K9W7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660524.1
- ProteinModelPortal:   Q8K9W7
- SMR:   Q8K9W7
- EnsemblBacteria:   EBBUCT00000000144
- GeneID:   1005365
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg168
- GeneTree:   EBGT00050000008132
- HOGENOM:   HBG351567
- OMA:   GLNKRRV
- ProtClustDB:   PRK00768
- BioCyc:   BAPH198804:BUSG168-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30605; Mature: 30474

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQKKIIELLGVKPLIIPKKEIENRIQILKRYLIENTHLKTLIVGISGGQDSTLTGKLC
CCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHH
QLSIQELRKEKKEKSYQFIALRLPYGVQIDEKDCRDAINFINPDQIFTINIKNAVLNSER
HHHHHHHHHHHHHCCEEEEEEECCCCCEECHHHHHHHHCCCCCCCEEEEEEHHHHHCCHH
SLKKQGIQISDYIKGNEKARERMKVQYSFAAITNGLVVGTGNAAENVTGFFTKYGDNGTD
HHHHCCCEEEHHHCCCHHHHHHHHHHEEHHHHHCCEEEECCCCHHHHHHHHEEECCCCCC
VNLISKLNKRQGKFLLKELNCPKHLYLKKPTADLEDEKPQKEDEVALGIKYNIIDDYLEG
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEEHHHHHCC
KKVNSLNKQIIERLYLTTEHKRKIINLG
CCHHHHHHHHHHHHHHCCHHHHHEECCC
>Mature Secondary Structure 
TLQKKIIELLGVKPLIIPKKEIENRIQILKRYLIENTHLKTLIVGISGGQDSTLTGKLC
CHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHH
QLSIQELRKEKKEKSYQFIALRLPYGVQIDEKDCRDAINFINPDQIFTINIKNAVLNSER
HHHHHHHHHHHHHCCEEEEEEECCCCCEECHHHHHHHHCCCCCCCEEEEEEHHHHHCCHH
SLKKQGIQISDYIKGNEKARERMKVQYSFAAITNGLVVGTGNAAENVTGFFTKYGDNGTD
HHHHCCCEEEHHHCCCHHHHHHHHHHEEHHHHHCCEEEECCCCHHHHHHHHEEECCCCCC
VNLISKLNKRQGKFLLKELNCPKHLYLKKPTADLEDEKPQKEDEVALGIKYNIIDDYLEG
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEEHHHHHCC
KKVNSLNKQIIERLYLTTEHKRKIINLG
CCHHHHHHHHHHHHHHCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438