Click here to switch to the map view.

The map label for this gene is nrdB

Identifier: 21672460

GI number: 21672460

Start: 192782

End: 193912

Strand: Reverse

Name: nrdB

Synonym: BUsg172

Alternate gene names: 21672460

Gene position: 193912-192782 (Counterclockwise)

Preceding gene: 21672461

Following gene: 21672457

Centisome position: 30.23

GC content: 26.17

Gene sequence:

>1131_bases
GTGTCTTATACAACTTTTTCAAAAAAGAAAAACAATCAACTTAAAGAACCGATGTTTTTTGGACAATCTGTAAATATAGC
TCGATATGACCAACAAAAATATAACATTTTTGAAAAATTAATTGAAAAGCAACTTTCTTTTTTTTGGAGACCAGAAGAAA
TAGATTTATCTAAAGATAGAATAGATTTTGATAATTTACCTTCTAACGAAAAACACATTTTTATAAGTAATTTAAAATAT
CAAACTTTACTCGATTCTATTCAAGGAAGAAGTCCTAATATCGCTTTTTTACCTATAGTTTCTATTCCTGAATTAGAAAC
GTGGATTGAAACATGGTCTTTTTCAGAAACAATTCATTCGCGTTCATATACACACATTATTAGAAATATTATTAATAATC
CATCTATCGTATTTGATGATATTATTTCTAATAAAAACATTAATGATCGCGCACAAGACATTTCCATTTATTATGATGAA
TTAATCAGAATAACAGCGTATTGGCATTTACTCGGTGAAGGAAATCATAAAATTAATGAAAAAAAAGTTGAAATTAATTT
AAAATTATTAAAAAGAAGATTGTATCTTTGTTTAATTAGTGTAAATGTATTAGAAGCTATCAGATTTTACGTTAGTTTTG
CGTGTTCTTTTGCATTCGCTGAAAGAGAAATCATGGAAGGAAATGCCAAAATTATTAGATTAATAGCACGCGATGAGGCA
TTACACTTAACTGGAACTCAGCATATTTTAAATATTTTAAATAATGAAAAAAATAATGAAAACATGAAAAATACCGTTTT
AGAATGCAGAGAAGAAGCAATTAAAATATTTATATCTGCTTCAGAACAAGAAAAAAAATGGGCTAAATATTTATTTCAAG
ATGGTTCAATGTTAGGATTGAATAAAGATATTTTATGTCAATATATAGAATATATTACCAACATTCGTATGCATGCAATA
GGTTTTAAAATGCCTTTTGAAAAAACATCAAATCCAATTCCTTGGATTAACTCTTGGTTGAACTCTGATTATATACAAGC
TGCTCCTCAAGAAACAGAAATTAGCTCATATTTAGTAGGTCAAATCGAATCAGAAATATCTAATCAAGAATTTAAAAATT
TTAAATTATAA

Upstream 100 bases:

>100_bases
TTGAAAACATAAAAGAAGATAATTGTACAAGTGGCTCTTGTACGATATAATAATGATATAAATTTAATTAAAAATTAAAA
ATTTTTCGGATATAACATAA

Downstream 100 bases:

>100_bases
AATGAATAATTTTACTATTAAAGTACTCAATAAAAAAACAATAATTTATAAAAAAAAAAATCTTTATTATCAATTTTAGA
AAAACATAACATAAAAATTG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSYTTFSKKKNNQLKEPMFFGQSVNIARYDQQKYNIFEKLIEKQLSFFWRPEEIDLSKDRIDFDNLPSNEKHIFISNLKY
QTLLDSIQGRSPNIAFLPIVSIPELETWIETWSFSETIHSRSYTHIIRNIINNPSIVFDDIISNKNINDRAQDISIYYDE
LIRITAYWHLLGEGNHKINEKKVEINLKLLKRRLYLCLISVNVLEAIRFYVSFACSFAFAEREIMEGNAKIIRLIARDEA
LHLTGTQHILNILNNEKNNENMKNTVLECREEAIKIFISASEQEKKWAKYLFQDGSMLGLNKDILCQYIEYITNIRMHAI
GFKMPFEKTSNPIPWINSWLNSDYIQAAPQETEISSYLVGQIESEISNQEFKNFKL

Sequences:

>Translated_376_residues
MSYTTFSKKKNNQLKEPMFFGQSVNIARYDQQKYNIFEKLIEKQLSFFWRPEEIDLSKDRIDFDNLPSNEKHIFISNLKY
QTLLDSIQGRSPNIAFLPIVSIPELETWIETWSFSETIHSRSYTHIIRNIINNPSIVFDDIISNKNINDRAQDISIYYDE
LIRITAYWHLLGEGNHKINEKKVEINLKLLKRRLYLCLISVNVLEAIRFYVSFACSFAFAEREIMEGNAKIIRLIARDEA
LHLTGTQHILNILNNEKNNENMKNTVLECREEAIKIFISASEQEKKWAKYLFQDGSMLGLNKDILCQYIEYITNIRMHAI
GFKMPFEKTSNPIPWINSWLNSDYIQAAPQETEISSYLVGQIESEISNQEFKNFKL
>Mature_375_residues
SYTTFSKKKNNQLKEPMFFGQSVNIARYDQQKYNIFEKLIEKQLSFFWRPEEIDLSKDRIDFDNLPSNEKHIFISNLKYQ
TLLDSIQGRSPNIAFLPIVSIPELETWIETWSFSETIHSRSYTHIIRNIINNPSIVFDDIISNKNINDRAQDISIYYDEL
IRITAYWHLLGEGNHKINEKKVEINLKLLKRRLYLCLISVNVLEAIRFYVSFACSFAFAEREIMEGNAKIIRLIARDEAL
HLTGTQHILNILNNEKNNENMKNTVLECREEAIKIFISASEQEKKWAKYLFQDGSMLGLNKDILCQYIEYITNIRMHAIG
FKMPFEKTSNPIPWINSWLNSDYIQAAPQETEISSYLVGQIESEISNQEFKNFKL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family

Homologues:

Organism=Escherichia coli, GI1788567, Length=376, Percent_Identity=72.0744680851064, Blast_Score=609, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR2_BUCAP (Q8K9W4)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660527.1
- ProteinModelPortal:   Q8K9W4
- SMR:   Q8K9W4
- EnsemblBacteria:   EBBUCT00000000324
- GeneID:   1005369
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg172
- GeneTree:   EBGT00050000007938
- HOGENOM:   HBG296569
- OMA:   QHMLNLM
- ProtClustDB:   PRK09101
- BioCyc:   BAPH198804:BUSG172-MONOMER
- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358
- Gene3D:   G3DSA:1.10.620.20
- PANTHER:   PTHR23409

Pfam domain/function: PF00268 Ribonuc_red_sm; SSF47240 Ferritin/RR_like

EC number: =1.17.4.1

Molecular weight: Translated: 44225; Mature: 44094

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00368 RIBORED_SMALL

Important sites: ACT_SITE 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTTFSKKKNNQLKEPMFFGQSVNIARYDQQKYNIFEKLIEKQLSFFWRPEEIDLSKDR
CCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDFDNLPSNEKHIFISNLKYQTLLDSIQGRSPNIAFLPIVSIPELETWIETWSFSETIHS
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIINNPSIVFDDIISNKNINDRAQDISIYYDELIRITAYWHLLGEGNHKINE
HHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
KKVEINLKLLKRRLYLCLISVNVLEAIRFYVSFACSFAFAEREIMEGNAKIIRLIARDEA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCE
LHLTGTQHILNILNNEKNNENMKNTVLECREEAIKIFISASEQEKKWAKYLFQDGSMLGL
EEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCEECC
NKDILCQYIEYITNIRMHAIGFKMPFEKTSNPIPWINSWLNSDYIQAAPQETEISSYLVG
CHHHHHHHHHHHHCCHHHEEEEECCHHCCCCCCCHHHHHCCCCHHHCCCCCHHHHHHHHH
QIESEISNQEFKNFKL
HHHHHHCHHHHHCCCC
>Mature Secondary Structure 
SYTTFSKKKNNQLKEPMFFGQSVNIARYDQQKYNIFEKLIEKQLSFFWRPEEIDLSKDR
CCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
IDFDNLPSNEKHIFISNLKYQTLLDSIQGRSPNIAFLPIVSIPELETWIETWSFSETIHS
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RSYTHIIRNIINNPSIVFDDIISNKNINDRAQDISIYYDELIRITAYWHLLGEGNHKINE
HHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCC
KKVEINLKLLKRRLYLCLISVNVLEAIRFYVSFACSFAFAEREIMEGNAKIIRLIARDEA
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEECCCE
LHLTGTQHILNILNNEKNNENMKNTVLECREEAIKIFISASEQEKKWAKYLFQDGSMLGL
EEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCCEECC
NKDILCQYIEYITNIRMHAIGFKMPFEKTSNPIPWINSWLNSDYIQAAPQETEISSYLVG
CHHHHHHHHHHHHCCHHHEEEEECCHHCCCCCCCHHHHHCCCCHHHCCCCCHHHHHHHHH
QIESEISNQEFKNFKL
HHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438