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The map label for this gene is ipk

Identifier: 21672453

GI number: 21672453

Start: 184078

End: 184956

Strand: Reverse

Name: ipk

Synonym: BUsg164

Alternate gene names: 21672453

Gene position: 184956-184078 (Counterclockwise)

Preceding gene: 21672457

Following gene: 21672452

Centisome position: 28.83

GC content: 25.94

Gene sequence:

>879_bases
ATGATAAATACATGGCCATCTCCTGCAAAAATTAATTTATTTCTTTATGTCACTGGTATTCGTTCAGATGGATATCATTA
TATACAAAGCTTATTTCAATTTCTTAACTATGGTGATACGTTGACGATTATTCCAAACACAAAAGGTACTATTGAGCTAT
TTACTGAAAGTAATTCACTTGTAAACATAAAAAATAGCATCATTACTGCAGCTGAATTATTAAAAGAAAAAGCATTACAT
TCTTTGGGAAAAAAAACTTCTCATTTTGGAGCAAAAATCTTTTTAAATAAAAAAATACCTATTGGAAGCGGATTAGGAGG
AGGTTCATCTAATGCTGCAACTACATTAGTAGTATTAAATAATTTATGGAAAACAAAATTTACTTTACAAGAATTAGCTG
AATTAAGTTTAAAAATAGGATCAGATATACCTGCTTTTATCATGGGAAAAACAACAATTGTTGAAGGAATAGGAGAAATT
TTATATCCTATTCATAGAAAAGAAAAATGGTATTTAATTGTTTATCCTAATATTTCAATATCAACAAAAAATATATTTTC
TATTTACTCTATTAAAAAAAATACAATAAAAAAACCGATTGATTTTTTATTAAGATCACGTTTTCATAACGATTTTGAAA
ACGTAATTAAAAAAAAATTTAAAAAAATAAAACAATTAATTTCTATGTTATCTCTTTATGCCCCTTCACGTATTACTGGA
ACAGGATCATGTATTTTTGCTGAATTTAATGACAAAAAATCTGCACAAAAAATACATTCTCTGCTTCCTCACAACGTTCA
AGGAACTATTGTGAAAAGTGTCAACGTTTCACCATTACATCATGCTTTTTATAAAAAAAACATAAATCTTTTTAACTAA

Upstream 100 bases:

>100_bases
AATTTTCAATTAAAATGATTATATCATTTTTAACATTTTTCAGTATAATTTTAACTAACATGAATATAAAAAATAATTAT
TTTAAGATACAAATATTAAA

Downstream 100 bases:

>100_bases
AAACTAAATTAGTTTAATGTGGAAATAATTAAAAAACACGCTTTTAAATAAAAAAAACAAGGTTTTTTTATGCCAGATAT
GAAATTATTTGCTGGAAACT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MINTWPSPAKINLFLYVTGIRSDGYHYIQSLFQFLNYGDTLTIIPNTKGTIELFTESNSLVNIKNSIITAAELLKEKALH
SLGKKTSHFGAKIFLNKKIPIGSGLGGGSSNAATTLVVLNNLWKTKFTLQELAELSLKIGSDIPAFIMGKTTIVEGIGEI
LYPIHRKEKWYLIVYPNISISTKNIFSIYSIKKNTIKKPIDFLLRSRFHNDFENVIKKKFKKIKQLISMLSLYAPSRITG
TGSCIFAEFNDKKSAQKIHSLLPHNVQGTIVKSVNVSPLHHAFYKKNINLFN

Sequences:

>Translated_292_residues
MINTWPSPAKINLFLYVTGIRSDGYHYIQSLFQFLNYGDTLTIIPNTKGTIELFTESNSLVNIKNSIITAAELLKEKALH
SLGKKTSHFGAKIFLNKKIPIGSGLGGGSSNAATTLVVLNNLWKTKFTLQELAELSLKIGSDIPAFIMGKTTIVEGIGEI
LYPIHRKEKWYLIVYPNISISTKNIFSIYSIKKNTIKKPIDFLLRSRFHNDFENVIKKKFKKIKQLISMLSLYAPSRITG
TGSCIFAEFNDKKSAQKIHSLLPHNVQGTIVKSVNVSPLHHAFYKKNINLFN
>Mature_292_residues
MINTWPSPAKINLFLYVTGIRSDGYHYIQSLFQFLNYGDTLTIIPNTKGTIELFTESNSLVNIKNSIITAAELLKEKALH
SLGKKTSHFGAKIFLNKKIPIGSGLGGGSSNAATTLVVLNNLWKTKFTLQELAELSLKIGSDIPAFIMGKTTIVEGIGEI
LYPIHRKEKWYLIVYPNISISTKNIFSIYSIKKNTIKKPIDFLLRSRFHNDFENVIKKKFKKIKQLISMLSLYAPSRITG
TGSCIFAEFNDKKSAQKIHSLLPHNVQGTIVKSVNVSPLHHAFYKKNINLFN

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=283, Percent_Identity=46.643109540636, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_BUCAP (Q8K9X1)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660520.1
- ProteinModelPortal:   Q8K9X1
- SMR:   Q8K9X1
- EnsemblBacteria:   EBBUCT00000000235
- GeneID:   1005361
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg164
- GeneTree:   EBGT00050000008174
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK00343
- BioCyc:   BAPH198804:BUSG164-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32820; Mature: 32820

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: ACT_SITE 10-10 ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINTWPSPAKINLFLYVTGIRSDGYHYIQSLFQFLNYGDTLTIIPNTKGTIELFTESNSL
CCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCE
VNIKNSIITAAELLKEKALHSLGKKTSHFGAKIFLNKKIPIGSGLGGGSSNAATTLVVLN
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHEEEEEEH
NLWKTKFTLQELAELSLKIGSDIPAFIMGKTTIVEGIGEILYPIHRKEKWYLIVYPNISI
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEE
STKNIFSIYSIKKNTIKKPIDFLLRSRFHNDFENVIKKKFKKIKQLISMLSLYAPSRITG
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TGSCIFAEFNDKKSAQKIHSLLPHNVQGTIVKSVNVSPLHHAFYKKNINLFN
CCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MINTWPSPAKINLFLYVTGIRSDGYHYIQSLFQFLNYGDTLTIIPNTKGTIELFTESNSL
CCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCE
VNIKNSIITAAELLKEKALHSLGKKTSHFGAKIFLNKKIPIGSGLGGGSSNAATTLVVLN
EEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHEEEEEEH
NLWKTKFTLQELAELSLKIGSDIPAFIMGKTTIVEGIGEILYPIHRKEKWYLIVYPNISI
HHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCEE
STKNIFSIYSIKKNTIKKPIDFLLRSRFHNDFENVIKKKFKKIKQLISMLSLYAPSRITG
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TGSCIFAEFNDKKSAQKIHSLLPHNVQGTIVKSVNVSPLHHAFYKKNINLFN
CCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438