Click here to switch to the map view.

The map label for this gene is prsA [H]

Identifier: 21672452

GI number: 21672452

Start: 183061

End: 184008

Strand: Reverse

Name: prsA [H]

Synonym: BUsg163

Alternate gene names: 21672452

Gene position: 184008-183061 (Counterclockwise)

Preceding gene: 21672453

Following gene: 21672436

Centisome position: 28.69

GC content: 33.86

Gene sequence:

>948_bases
ATGCCAGATATGAAATTATTTGCTGGAAACTCTATTCCAAAATTAGCAAAATTTATTGCTCATCGACTTTATATAAATTT
AGGAAACGCAGCTGTAGGTAGATTTAGTGATGGTGAAATTAGTGTTCAAATAAATGAAAATGTTAGAGGTAGCGACGTTT
TCATAATTCAGTCTACTTGCTCTCCTACTAATGACAATATTATGGAATTAGTCGTGATGGTAGATGCTTTAAGAAGAGCA
TCAGCTGGTAGAATTACAGCTGTAATACCTTATTTTGGGTATTCTCGTCAAGATCGTCGTGTAAGATCAGCACGTGTGCC
AATTACTGCTAAGGTAGTCGCTGATTTTTTATCCAGTATTGGAGTTGATCGAGTACTGACAGTAGATCTTCATGCAGAAC
AGATACAAGGTTTTTTTGACGTACCTGTAGATAATGTTTTTGGAAGTTTAATTCTTTTAGAAGATATGCTACAAAGAGAA
TTAAAAAATCCAATTGTTGTTTCACCAGATATTGGAGGAGTAGTAAGAGCTCGAGCTATTGCTAAACTTCTTTATGACAC
AGATATGGCTATTATAGACAAAAGGAGGCCTCGTCCTAACATTTCGCAAATCATGCACATTATTGGTGATGTAGCAAATA
GAGATTGTATTTTGGTTGATGACATGATTGATACAGGAGGAACACTTTGCAAAGCAGCTGAAGCTCTTAAAGAAAGAGGA
GCTAAAAGAGTTTTTGCATATGCTACACATCCTATTTTTTCTGGAAAAGCATCAGAAAATTTAAAAAAATCTGTTATTGA
TGAAGTAGTTGTATGTGATACTATTCCATTAGAAGAAAAAATTAGATTATTACCAAATGTAAGAACTTTAACGTTAGCAG
GTATGCTAGCAGAAGCTATAAGACGAATTAGTAATGAAGAATCTATTTCTGCTATGTTTGAACATTAA

Upstream 100 bases:

>100_bases
TTATAAAAAAAACATAAATCTTTTTAACTAAAAACTAAATTAGTTTAATGTGGAAATAATTAAAAAACACGCTTTTAAAT
AAAAAAAACAAGGTTTTTTT

Downstream 100 bases:

>100_bases
GAATTTTTTTAAAATAACTTTAATAATCCTAAGCACACTATATTTCTTATTAATGTGCTTAGCATTTTCAAAAAATTATT
AAAATCACTAGAAATTTAAT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPDMKLFAGNSIPKLAKFIAHRLYINLGNAAVGRFSDGEISVQINENVRGSDVFIIQSTCSPTNDNIMELVVMVDALRRA
SAGRITAVIPYFGYSRQDRRVRSARVPITAKVVADFLSSIGVDRVLTVDLHAEQIQGFFDVPVDNVFGSLILLEDMLQRE
LKNPIVVSPDIGGVVRARAIAKLLYDTDMAIIDKRRPRPNISQIMHIIGDVANRDCILVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGKASENLKKSVIDEVVVCDTIPLEEKIRLLPNVRTLTLAGMLAEAIRRISNEESISAMFEH

Sequences:

>Translated_315_residues
MPDMKLFAGNSIPKLAKFIAHRLYINLGNAAVGRFSDGEISVQINENVRGSDVFIIQSTCSPTNDNIMELVVMVDALRRA
SAGRITAVIPYFGYSRQDRRVRSARVPITAKVVADFLSSIGVDRVLTVDLHAEQIQGFFDVPVDNVFGSLILLEDMLQRE
LKNPIVVSPDIGGVVRARAIAKLLYDTDMAIIDKRRPRPNISQIMHIIGDVANRDCILVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGKASENLKKSVIDEVVVCDTIPLEEKIRLLPNVRTLTLAGMLAEAIRRISNEESISAMFEH
>Mature_314_residues
PDMKLFAGNSIPKLAKFIAHRLYINLGNAAVGRFSDGEISVQINENVRGSDVFIIQSTCSPTNDNIMELVVMVDALRRAS
AGRITAVIPYFGYSRQDRRVRSARVPITAKVVADFLSSIGVDRVLTVDLHAEQIQGFFDVPVDNVFGSLILLEDMLQREL
KNPIVVSPDIGGVVRARAIAKLLYDTDMAIIDKRRPRPNISQIMHIIGDVANRDCILVDDMIDTGGTLCKAAEALKERGA
KRVFAYATHPIFSGKASENLKKSVIDEVVVCDTIPLEEKIRLLPNVRTLTLAGMLAEAIRRISNEESISAMFEH

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=317, Percent_Identity=47.0031545741325, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI4506129, Length=317, Percent_Identity=47.0031545741325, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI84875539, Length=319, Percent_Identity=46.7084639498433, Blast_Score=288, Evalue=4e-78,
Organism=Homo sapiens, GI28557709, Length=317, Percent_Identity=47.0031545741325, Blast_Score=287, Evalue=9e-78,
Organism=Homo sapiens, GI4506133, Length=344, Percent_Identity=36.9186046511628, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=36.046511627907, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI310128524, Length=146, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI310115209, Length=146, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI310118259, Length=146, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI310119946, Length=146, Percent_Identity=30.1369863013699, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=88.2539682539683, Blast_Score=573, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25149168, Length=317, Percent_Identity=44.794952681388, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=44.794952681388, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI71989924, Length=317, Percent_Identity=44.794952681388, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=44.5512820512821, Blast_Score=271, Evalue=4e-73,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=34.9112426035503, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321776, Length=324, Percent_Identity=46.2962962962963, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6319403, Length=316, Percent_Identity=45.8860759493671, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=44.7619047619048, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6322667, Length=206, Percent_Identity=41.747572815534, Blast_Score=148, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6324511, Length=271, Percent_Identity=32.4723247232472, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355239, Length=317, Percent_Identity=46.3722397476341, Blast_Score=280, Evalue=8e-76,
Organism=Drosophila melanogaster, GI45551540, Length=339, Percent_Identity=43.3628318584071, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24651458, Length=355, Percent_Identity=34.3661971830986, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24651456, Length=355, Percent_Identity=34.3661971830986, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI281362873, Length=355, Percent_Identity=34.3661971830986, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24651454, Length=355, Percent_Identity=34.3661971830986, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24651462, Length=374, Percent_Identity=32.620320855615, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24651464, Length=374, Percent_Identity=32.620320855615, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI45552010, Length=374, Percent_Identity=32.620320855615, Blast_Score=188, Evalue=4e-48,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34760; Mature: 34629

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDMKLFAGNSIPKLAKFIAHRLYINLGNAAVGRFSDGEISVQINENVRGSDVFIIQSTC
CCCCEEECCCCHHHHHHHHHHHHHEEECCCEECCCCCCEEEEEECCCCCCCEEEEEECCC
SPTNDNIMELVVMVDALRRASAGRITAVIPYFGYSRQDRRVRSARVPITAKVVADFLSSI
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHC
GVDRVLTVDLHAEQIQGFFDVPVDNVFGSLILLEDMLQRELKNPIVVSPDIGGVVRARAI
CCCEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
AKLLYDTDMAIIDKRRPRPNISQIMHIIGDVANRDCILVDDMIDTGGTLCKAAEALKERG
HHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECHHCCCCHHHHHHHHHHHCC
AKRVFAYATHPIFSGKASENLKKSVIDEVVVCDTIPLEEKIRLLPNVRTLTLAGMLAEAI
CCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
RRISNEESISAMFEH
HHHCCHHHHHHHHCC
>Mature Secondary Structure 
PDMKLFAGNSIPKLAKFIAHRLYINLGNAAVGRFSDGEISVQINENVRGSDVFIIQSTC
CCCEEECCCCHHHHHHHHHHHHHEEECCCEECCCCCCEEEEEECCCCCCCEEEEEECCC
SPTNDNIMELVVMVDALRRASAGRITAVIPYFGYSRQDRRVRSARVPITAKVVADFLSSI
CCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHC
GVDRVLTVDLHAEQIQGFFDVPVDNVFGSLILLEDMLQRELKNPIVVSPDIGGVVRARAI
CCCEEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
AKLLYDTDMAIIDKRRPRPNISQIMHIIGDVANRDCILVDDMIDTGGTLCKAAEALKERG
HHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECHHCCCCHHHHHHHHHHHCC
AKRVFAYATHPIFSGKASENLKKSVIDEVVVCDTIPLEEKIRLLPNVRTLTLAGMLAEAI
CCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
RRISNEESISAMFEH
HHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]