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The map label for this gene is ptsI

Identifier: 21672354

GI number: 21672354

Start: 69106

End: 70818

Strand: Reverse

Name: ptsI

Synonym: BUsg061

Alternate gene names: 21672354

Gene position: 70818-69106 (Counterclockwise)

Preceding gene: 21672355

Following gene: 21672353

Centisome position: 11.04

GC content: 27.26

Gene sequence:

>1713_bases
ATGATTTCAGGCATTTTAGCATCACCCGGTATAGCTTTTGGCAATGTACTTTTATTAAAAAAAGAAGAAATTGTCATTAA
CCGGCAAAATATTTCTACTGAAAATATAAAAACAGAAATAAATAAGTTTTTTAACGGTAGAAAAAAATCAGTAAAACAAC
TAACAGAAATAAAAATAGCAACAGGCGAAAAATTTGGAAAGAAAAAAGAAGGTATCTTCGAAGGTCATATAATGCTTCTT
GAAGACGAAGAACTGGAAAAAGAAATAATCAACTTAATAACAGAAAAAAAAATATCTGCTGCAGAAGCAACAAAATTCAT
TATTGAAGGACAAGCAAAAGCATTAGAAAAAATAAAAGACGAATATTTAAAAAACAGAGCAATTGATGTAAGAGATATTG
GTTCTCGTTTACTAAAAAATATACTTAATATTAGTATTGTTGATTTAAATAATATAAAAAATCAAGTTATTTTAATTTCA
AAAGATCTCACTCCTTCAGAAACAGCACAAATCAATTTAAAATATATTTTAGGATTTATTACAGATTTAGGCGGTCCAAC
TTCACATACATCCATTATGGCAAGATCACTTGAAATACCAGCTATTGTAGGTACTGTAAATATAACTAAAAAGGTAAAAA
ACAACGATTTCATTATTTTAGATTCTCTTAATAATGAAATTATTATCAATCCATCTGATCAAATAATAAATGAAAAAAAA
CAAGTTGAAAGAAAATATTTTTTTAAGAAAGAAAATTTAAAAAAATTAAGAAATTTATATGCAACTACTACTGATGGACA
CAATATTAAAATTGGATCTAATATTGGCAATATTCAAGATATTTATTCAGCAAAAAAAAATGGTGCAGAATGTATAGGTT
TGTATAGAACAGAATTTTTATTTATGGGCAGAAATGCACTTCCTACTGAAAACGAACAATTTCAAGCATATAAAGAAATT
GCAGAATCAATGGAAAACAAACCAGTCATTATCAGAACTATGGATATCGGTGGTGATAAAGATCTTCCGTACATGAATTT
ACCAAAAGAAGAAAATCCTTTTCTAGGATGGAGAGCTATACGTATTTCAATGGATCGTAAGGAAATTTTACATGCACAAT
TAAGAGCAATTCTTAGAGCTTCTGCATTTGGTAAAATTTATATTTTATTTCCTATGATTATATCGGTAGAAGAAATTAGA
ACATTAAAAATTGAAGTTCATAAATTGCAAATTCAGTTAAAAAAAGAGCATTTGGTATTCGATGAGAATATTAAAATTGG
CATCATGATAGAAACTCCTGCATCAGCGATAATAGCAGATCATCTAATAAAAGAAGTTGATTTTTTTAGTATTGGTACTA
ATGATTTAACACAATATACCTTGGCAGTAGACAGAGGAAATGATTTAATTTCACATCTTTACAATCCTATAAGTCCCTCT
GTTATACAACTAATTAAACAAGTTATTGATATATCACATTTACATGGAAAATGGACAGGTATGTGCGGAGAATTAGCAGG
AGATGAACGCGTTACAACACTTTTGTTAGGGATGGGTTTAGATGAATTTAGTATGAGTTCAACAAGTATTCCTAAAATTA
AAGAAATTATTCGTAAAACATCTTTTTCAAAATCTCAAGAATTAGCTAAAAAGGTATTAAAAGCAGCAACTACAAAAGAA
ATTTTAGATTTATTAAATAAATTCATTATTTAA

Upstream 100 bases:

>100_bases
TTTACATTACTTCCGGATTTATTTTATAATAAATAAAATATATTCGCCTGACATTCTATTTTTTCCCTCTCTAAACAAAT
TTTATGGTTAAGGTAATGTT

Downstream 100 bases:

>100_bases
GGATAGATGAAATTTTAAAAAAAATTTAATTTACAGAAGGACTTAGGAGATAAAAATGAGTTTCTTTTCTGGTTTTTTTA
ATAAAAAAAAATCTAAATAT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MISGILASPGIAFGNVLLLKKEEIVINRQNISTENIKTEINKFFNGRKKSVKQLTEIKIATGEKFGKKKEGIFEGHIMLL
EDEELEKEIINLITEKKISAAEATKFIIEGQAKALEKIKDEYLKNRAIDVRDIGSRLLKNILNISIVDLNNIKNQVILIS
KDLTPSETAQINLKYILGFITDLGGPTSHTSIMARSLEIPAIVGTVNITKKVKNNDFIILDSLNNEIIINPSDQIINEKK
QVERKYFFKKENLKKLRNLYATTTDGHNIKIGSNIGNIQDIYSAKKNGAECIGLYRTEFLFMGRNALPTENEQFQAYKEI
AESMENKPVIIRTMDIGGDKDLPYMNLPKEENPFLGWRAIRISMDRKEILHAQLRAILRASAFGKIYILFPMIISVEEIR
TLKIEVHKLQIQLKKEHLVFDENIKIGIMIETPASAIIADHLIKEVDFFSIGTNDLTQYTLAVDRGNDLISHLYNPISPS
VIQLIKQVIDISHLHGKWTGMCGELAGDERVTTLLLGMGLDEFSMSSTSIPKIKEIIRKTSFSKSQELAKKVLKAATTKE
ILDLLNKFII

Sequences:

>Translated_570_residues
MISGILASPGIAFGNVLLLKKEEIVINRQNISTENIKTEINKFFNGRKKSVKQLTEIKIATGEKFGKKKEGIFEGHIMLL
EDEELEKEIINLITEKKISAAEATKFIIEGQAKALEKIKDEYLKNRAIDVRDIGSRLLKNILNISIVDLNNIKNQVILIS
KDLTPSETAQINLKYILGFITDLGGPTSHTSIMARSLEIPAIVGTVNITKKVKNNDFIILDSLNNEIIINPSDQIINEKK
QVERKYFFKKENLKKLRNLYATTTDGHNIKIGSNIGNIQDIYSAKKNGAECIGLYRTEFLFMGRNALPTENEQFQAYKEI
AESMENKPVIIRTMDIGGDKDLPYMNLPKEENPFLGWRAIRISMDRKEILHAQLRAILRASAFGKIYILFPMIISVEEIR
TLKIEVHKLQIQLKKEHLVFDENIKIGIMIETPASAIIADHLIKEVDFFSIGTNDLTQYTLAVDRGNDLISHLYNPISPS
VIQLIKQVIDISHLHGKWTGMCGELAGDERVTTLLLGMGLDEFSMSSTSIPKIKEIIRKTSFSKSQELAKKVLKAATTKE
ILDLLNKFII
>Mature_570_residues
MISGILASPGIAFGNVLLLKKEEIVINRQNISTENIKTEINKFFNGRKKSVKQLTEIKIATGEKFGKKKEGIFEGHIMLL
EDEELEKEIINLITEKKISAAEATKFIIEGQAKALEKIKDEYLKNRAIDVRDIGSRLLKNILNISIVDLNNIKNQVILIS
KDLTPSETAQINLKYILGFITDLGGPTSHTSIMARSLEIPAIVGTVNITKKVKNNDFIILDSLNNEIIINPSDQIINEKK
QVERKYFFKKENLKKLRNLYATTTDGHNIKIGSNIGNIQDIYSAKKNGAECIGLYRTEFLFMGRNALPTENEQFQAYKEI
AESMENKPVIIRTMDIGGDKDLPYMNLPKEENPFLGWRAIRISMDRKEILHAQLRAILRASAFGKIYILFPMIISVEEIR
TLKIEVHKLQIQLKKEHLVFDENIKIGIMIETPASAIIADHLIKEVDFFSIGTNDLTQYTLAVDRGNDLISHLYNPISPS
VIQLIKQVIDISHLHGKWTGMCGELAGDERVTTLLLGMGLDEFSMSSTSIPKIKEIIRKTSFSKSQELAKKVLKAATTKE
ILDLLNKFII

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Escherichia coli, GI1788756, Length=569, Percent_Identity=64.4991212653779, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=573, Percent_Identity=36.6492146596859, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI48994992, Length=499, Percent_Identity=36.6733466933868, Blast_Score=328, Evalue=8e-91,
Organism=Escherichia coli, GI1789193, Length=576, Percent_Identity=30.7291666666667, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1787994, Length=452, Percent_Identity=25.6637168141593, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI226510935, Length=129, Percent_Identity=30.2325581395349, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PT1_BUCAP (Q8KA50)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660421.1
- ProteinModelPortal:   Q8KA50
- SMR:   Q8KA50
- EnsemblBacteria:   EBBUCT00000000168
- GeneID:   1005877
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg061
- GeneTree:   EBGT00050000007917
- HOGENOM:   HBG456539
- OMA:   MDREQLP
- ProtClustDB:   PRK11177
- BioCyc:   BAPH198804:BUSG061-MONOMER
- GO:   GO:0005737
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.50.30.10
- Gene3D:   G3DSA:1.10.274.10
- Gene3D:   G3DSA:3.20.20.60
- PRINTS:   PR01736
- TIGRFAMs:   TIGR01417

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; SSF47831 PEP-utilisers_N; SSF52009 PEP_mobile; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.3.9

Molecular weight: Translated: 64358; Mature: 64358

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00742 PEP_ENZYMES_2; PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: ACT_SITE 189-189 ACT_SITE 502-502 BINDING 296-296 BINDING 332-332 BINDING 431-431 BINDING 452-452 BINDING 453-453 BINDING 454-454 BINDING 455-455

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAFGNVLLLKKEEIVINRQNISTENIKTEINKFFNGRKKSVKQLTEIKIA
CCCCCCCCCCCCCCCEEEEEECEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHEEEE
TGEKFGKKKEGIFEGHIMLLEDEELEKEIINLITEKKISAAEATKFIIEGQAKALEKIKD
CCCCCCCCCCCEEECEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHEECCHHHHHHHHHH
EYLKNRAIDVRDIGSRLLKNILNISIVDLNNIKNQVILISKDLTPSETAQINLKYILGFI
HHHHCCCCCHHHHHHHHHHHHHCEEEEEECCCCCEEEEEECCCCCCCCCEEEHHHHHHHH
TDLGGPTSHTSIMARSLEIPAIVGTVNITKKVKNNDFIILDSLNNEIIINPSDQIINEKK
HHCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECCCCEEEECCCHHHHHHHH
QVERKYFFKKENLKKLRNLYATTTDGHNIKIGSNIGNIQDIYSAKKNGAECIGLYRTEFL
HHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHCCCCCCEEEHHHHEEE
FMGRNALPTENEQFQAYKEIAESMENKPVIIRTMDIGGDKDLPYMNLPKEENPFLGWRAI
EECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
RISMDRKEILHAQLRAILRASAFGKIYILFPMIISVEEIRTLKIEVHKLQIQLKKEHLVF
EEECCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEEEEEEEEEEEEECCCEEE
DENIKIGIMIETPASAIIADHLIKEVDFFSIGTNDLTQYTLAVDRGNDLISHLYNPISPS
ECCEEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCHHHHHHCCCCCHH
VIQLIKQVIDISHLHGKWTGMCGELAGDERVTTLLLGMGLDEFSMSSTSIPKIKEIIRKT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHC
SFSKSQELAKKVLKAATTKEILDLLNKFII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISGILASPGIAFGNVLLLKKEEIVINRQNISTENIKTEINKFFNGRKKSVKQLTEIKIA
CCCCCCCCCCCCCCCEEEEEECEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHEEEE
TGEKFGKKKEGIFEGHIMLLEDEELEKEIINLITEKKISAAEATKFIIEGQAKALEKIKD
CCCCCCCCCCCEEECEEEEEECHHHHHHHHHHHHHHCCCHHHHHHHEECCHHHHHHHHHH
EYLKNRAIDVRDIGSRLLKNILNISIVDLNNIKNQVILISKDLTPSETAQINLKYILGFI
HHHHCCCCCHHHHHHHHHHHHHCEEEEEECCCCCEEEEEECCCCCCCCCEEEHHHHHHHH
TDLGGPTSHTSIMARSLEIPAIVGTVNITKKVKNNDFIILDSLNNEIIINPSDQIINEKK
HHCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCCEEEEECCCCEEEECCCHHHHHHHH
QVERKYFFKKENLKKLRNLYATTTDGHNIKIGSNIGNIQDIYSAKKNGAECIGLYRTEFL
HHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHCCCCCCEEEHHHHEEE
FMGRNALPTENEQFQAYKEIAESMENKPVIIRTMDIGGDKDLPYMNLPKEENPFLGWRAI
EECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEE
RISMDRKEILHAQLRAILRASAFGKIYILFPMIISVEEIRTLKIEVHKLQIQLKKEHLVF
EEECCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEEEEEEEEEEEEECCCEEE
DENIKIGIMIETPASAIIADHLIKEVDFFSIGTNDLTQYTLAVDRGNDLISHLYNPISPS
ECCEEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCHHHHHHCCCCCHH
VIQLIKQVIDISHLHGKWTGMCGELAGDERVTTLLLGMGLDEFSMSSTSIPKIKEIIRKT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHC
SFSKSQELAKKVLKAATTKEILDLLNKFII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438