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The map label for this gene is crr

Identifier: 21672353

GI number: 21672353

Start: 68547

End: 69050

Strand: Reverse

Name: crr

Synonym: BUsg060

Alternate gene names: 21672353

Gene position: 69050-68547 (Counterclockwise)

Preceding gene: 21672354

Following gene: 21672352

Centisome position: 10.76

GC content: 25.2

Gene sequence:

>504_bases
ATGAGTTTCTTTTCTGGTTTTTTTAATAAAAAAAAATCTAAATATTCAAAAAAAATAGATATTTTAGCACCTTTATCAGG
TGATATAATCAATATTGAAGATGTACCAGATGTTGTTTTTTCTAAAAAAATTGTAGGTGATGGAATAGCTATTAATCCTA
CAGGAAATAAAATCCTTGCACCAATAAACGGTACAATTGGAAAGATATTAAATAGTATGCATGCTTTTTCAATTATTTCA
GAAGAAGGAGTAGAATTATTTGTGCATTTTGGCATTGATACAGTAAAATTAAGGGGAGAAGGTTTTAAACAAATAGCACA
AGATAATCAAAAAGTAAAAATAGGAGATACTATAATCTTATTTGATTTAAATTTATTAAAAGAAAAAGCTCGTTCTATCT
TAACCCCAGTTGTAATATCCAATATAGAAAAATTTAAAAACATAGAAAAGTCATCAGGTACAATTATTGCTGGAAAAACA
ATTATTATGTCTTTATATAATTGA

Upstream 100 bases:

>100_bases
ACTACAAAAGAAATTTTAGATTTATTAAATAAATTCATTATTTAAGGATAGATGAAATTTTAAAAAAAATTTAATTTACA
GAAGGACTTAGGAGATAAAA

Downstream 100 bases:

>100_bases
TTTTTAATATAAAAACGGTGCTATTATTTAGTACCGTTTTAACATCAAGGTGAGAAGTAAAAATGTTTTTTTTTCATAAG
ATAGTTATTCCTATTATTAT

Product: PTS system glucose-specific transporter subunit

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSFFSGFFNKKKSKYSKKIDILAPLSGDIINIEDVPDVVFSKKIVGDGIAINPTGNKILAPINGTIGKILNSMHAFSIIS
EEGVELFVHFGIDTVKLRGEGFKQIAQDNQKVKIGDTIILFDLNLLKEKARSILTPVVISNIEKFKNIEKSSGTIIAGKT
IIMSLYN

Sequences:

>Translated_167_residues
MSFFSGFFNKKKSKYSKKIDILAPLSGDIINIEDVPDVVFSKKIVGDGIAINPTGNKILAPINGTIGKILNSMHAFSIIS
EEGVELFVHFGIDTVKLRGEGFKQIAQDNQKVKIGDTIILFDLNLLKEKARSILTPVVISNIEKFKNIEKSSGTIIAGKT
IIMSLYN
>Mature_166_residues
SFFSGFFNKKKSKYSKKIDILAPLSGDIINIEDVPDVVFSKKIVGDGIAINPTGNKILAPINGTIGKILNSMHAFSIISE
EGVELFVHFGIDTVKLRGEGFKQIAQDNQKVKIGDTIILFDLNLLKEKARSILTPVVISNIEKFKNIEKSSGTIIAGKTI
IMSLYN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain

Homologues:

Organism=Escherichia coli, GI1788757, Length=166, Percent_Identity=62.0481927710843, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1786894, Length=142, Percent_Identity=44.3661971830986, Blast_Score=133, Evalue=6e-33,
Organism=Escherichia coli, GI1790159, Length=143, Percent_Identity=35.6643356643357, Blast_Score=100, Evalue=4e-23,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PTGA_BUCAP (Q8KA51)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660420.1
- ProteinModelPortal:   Q8KA51
- SMR:   Q8KA51
- EnsemblBacteria:   EBBUCT00000000319
- GeneID:   1005876
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg060
- GeneTree:   EBGT00050000008103
- HOGENOM:   HBG517865
- OMA:   RRLISGQ
- ProtClustDB:   PRK09439
- BioCyc:   BAPH198804:BUSG060-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR011055
- InterPro:   IPR001127
- TIGRFAMs:   TIGR00830

Pfam domain/function: PF00358 PTS_EIIA_1; SSF51261 Dup_hybrid_motif

EC number: 2.7.1.69

Molecular weight: Translated: 18329; Mature: 18198

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS51093 PTS_EIIA_TYPE_1; PS00371 PTS_EIIA_TYPE_1_HIS

Important sites: ACT_SITE 89-89

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFSGFFNKKKSKYSKKIDILAPLSGDIINIEDVPDVVFSKKIVGDGIAINPTGNKILA
CCHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCHHHHHHHHCCCCEEECCCCCEEEE
PINGTIGKILNSMHAFSIISEEGVELFVHFGIDTVKLRGEGFKQIAQDNQKVKIGDTIIL
ECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCCHHHHHCCCCEEEECCEEEE
FDLNLLKEKARSILTPVVISNIEKFKNIEKSSGTIIAGKTIIMSLYN
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEECC
>Mature Secondary Structure 
SFFSGFFNKKKSKYSKKIDILAPLSGDIINIEDVPDVVFSKKIVGDGIAINPTGNKILA
CHHHHHHHHHHHHHHCCEEEEECCCCCEEECCCCCHHHHHHHHCCCCEEECCCCCEEEE
PINGTIGKILNSMHAFSIISEEGVELFVHFGIDTVKLRGEGFKQIAQDNQKVKIGDTIIL
ECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCCHHHHHCCCCEEEECCEEEE
FDLNLLKEKARSILTPVVISNIEKFKNIEKSSGTIIAGKTIIMSLYN
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438