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The map label for this gene is hupA

Identifier: 21672326

GI number: 21672326

Start: 35721

End: 35993

Strand: Reverse

Name: hupA

Synonym: BUsg033

Alternate gene names: 21672326

Gene position: 35993-35721 (Counterclockwise)

Preceding gene: 21672327

Following gene: 21672325

Centisome position: 5.61

GC content: 26.37

Gene sequence:

>273_bases
ATGAATAAAACTCAATTAATTAAAATTATTTCTAAGCAATCTAATTTATCTAAAATACAGATTAAATCTACTTTAGAAAA
AATACTATCGGCTATCATTGAATCTTTAAAAAAAGGTGAATCAGTACAAATAGTAGGCTTTGGTACTTTTAAAGTAAATT
CAAGAGCATCTCGTACTGGAAGAAATCCTCAAACAGGAAAAGAAATAATCATCCCTGCAACAAAAGTCCCTAGTTTTATA
TCTGGAAAAACATTAAAAAATGCAATTAAGTAA

Upstream 100 bases:

>100_bases
AAATATGTAAATATTTATATTAAAAAAATTATTTGAAATTCTAAAATAAATTTTTTTATTTAAATTAATTAATACTTACT
TAAGTAAGAAAGGGAAAATA

Downstream 100 bases:

>100_bases
TTTATATTGCCAAAAAATGCAAAAGGGGCATTATGCCCCTAATAGTAATCTATTTTTAAAAAATAAAAATTTTTACATAT
AAAAAATATTAAAATTTATA

Product: DNA-binding protein HU-alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKTQLIKIISKQSNLSKIQIKSTLEKILSAIIESLKKGESVQIVGFGTFKVNSRASRTGRNPQTGKEIIIPATKVPSFI
SGKTLKNAIK

Sequences:

>Translated_90_residues
MNKTQLIKIISKQSNLSKIQIKSTLEKILSAIIESLKKGESVQIVGFGTFKVNSRASRTGRNPQTGKEIIIPATKVPSFI
SGKTLKNAIK
>Mature_90_residues
MNKTQLIKIISKQSNLSKIQIKSTLEKILSAIIESLKKGESVQIVGFGTFKVNSRASRTGRNPQTGKEIIIPATKVPSFI
SGKTLKNAIK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family

Homologues:

Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=66.6666666666667, Blast_Score=130, Evalue=2e-32,
Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=56.1797752808989, Blast_Score=109, Evalue=4e-26,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=35.2272727272727, Blast_Score=72, Evalue=9e-15,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=1e-11,

Paralogues:

None

Copy number: 1380 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): DBH_BUCAP (Q8KA69)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660393.1
- ProteinModelPortal:   Q8KA69
- SMR:   Q8KA69
- EnsemblBacteria:   EBBUCT00000000070
- GeneID:   1005849
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg033
- GeneTree:   EBGT00050000007762
- HOGENOM:   HBG705085
- OMA:   MNSITYE
- ProtClustDB:   CLSK315016
- BioCyc:   BAPH198804:BUSG033-MONOMER
- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992
- Gene3D:   G3DSA:4.10.520.10
- PRINTS:   PR01727
- SMART:   SM00411

Pfam domain/function: PF00216 Bac_DNA_binding; SSF47729 IHF_like_DNA_bnd

EC number: NA

Molecular weight: Translated: 9825; Mature: 9825

Theoretical pI: Translated: 11.51; Mature: 11.51

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTQLIKIISKQSNLSKIQIKSTLEKILSAIIESLKKGESVQIVGFGTFKVNSRASRTG
CCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCC
RNPQTGKEIIIPATKVPSFISGKTLKNAIK
CCCCCCCEEEEECCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MNKTQLIKIISKQSNLSKIQIKSTLEKILSAIIESLKKGESVQIVGFGTFKVNSRASRTG
CCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCC
RNPQTGKEIIIPATKVPSFISGKTLKNAIK
CCCCCCCEEEEECCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438