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The map label for this gene is purH

Identifier: 21672325

GI number: 21672325

Start: 33999

End: 35579

Strand: Reverse

Name: purH

Synonym: BUsg032

Alternate gene names: 21672325

Gene position: 35579-33999 (Counterclockwise)

Preceding gene: 21672326

Following gene: 21672323

Centisome position: 5.55

GC content: 26.69

Gene sequence:

>1581_bases
ATGTCATTAAATAATATTATAAAGAATGCTTTAGTTAGTTTGTCAGATAAAACAGATCTTTTAAAGATATCAAAGATACT
TGCAGAAAAAAAAATTAATTTATTTTGTACAGGAGGAACAGCTGACGTTTTAAAAAAAAATAAAATTCCTGTTCTTGAAA
TATCAGACTACACAAATTTTCCCGAAATAATGAATGGAAGACTCAAAACATTACACCCGAAAATAATAGGTGGTATTTTA
GGTCGAAGGGAAAAAGATCAAAAAATTATGAAATTACATAATTTAATACCTATAGATATAGTAATTGTGAATTTTTATCC
ATTTGAAAAAATACAAAATCGAAAAGAATTTACTATAGAAGAAATAATTGACAATATTGATATTGGTGGACCAACACTCG
TACGTGCTGCGGCTAAAAATTATAAAGATGTTATAGTTATAGTAGATTTATCAGATTTTACATCATGTATTCAATTAATA
AATACTAATACTGTAAGTTTAGAAACGAGATTTGATTTAGCTACTAAAGCTTTTAAGTACACTGCACTATATGAAGAAAT
AATTTCAAAATATTTTTTAAAAAAAAATCCTTATAGAAAAAAACATCAAAAAAATATTTTTCCAAATGAATTTCAATTAA
ATTTCATAAAAAAACAAGATTTAAGATATGGAGAAAATCAACATCAAAAATCTTCATTTTATATAGAAAAAGAAATATTA
AAATCTGGTACGATCAGTTCATCGAATCAAATACAAGGTAAAAATCTATCTTATAATAATATATGTGATGCTGATATAGC
ATTAGAATGCGTAAAAGAATTTTCTAAACCAACATGTGTTATTGTAAAACATGGAAATCCTTGCGGAGTTTCAGAAAGTA
ATTCTTTAATCAAAGCTTACTTCTCTGCTTATAATGCAGATCCGATTTCAGCTTTCGGAGGAATAATTGCTTTTAATTGT
CTCTTAGATTTAGACACAGCTCAAGAAATTGTTAAAAAACAGTTTGTCGAAGTTATTATTGCTCCTGAAATAGATGAAAT
GGCTGTAAAAATACTAAAAAGAAAAAAAAATATACGTTTGCTTATTTGCGGAAAAATTGAAAAAAATAAAAAAGGTTTAG
ATTTTAAAAGAATTACTAATGGTTTACTTATACAAGAATATGATTGTGATGAAATAAACGCAAAAAATTTTGATTTTGTT
ACAAATCGTTTACCTACTGAAAAAGAATTAGAAGATGCTATTTTTTCTTGGAAAGTCGCTAAATTTGTTAAATCAAACGC
TATTGTATACAGTTTAAATAAAACTACTATTGGAATAGGAGCAGGACAAACAAGCAGAATTGATGCAACTAAATTAGCAA
ACTTAAAAGTAAAAGATCGAAATCACAATAATACTACAGGAGCAACTATGGCTTCTGATGCTTTTTTCCCTTTTCGAGAC
GGGATAGATAATGCAGCTCTAATCGGTATTAGTTGTATTATCCAACCAGGTGGTTCTATTCGAGATAAAGAGGTAATTGA
GTCTGCCAATGAGCATAATATAAGTATGATTTTTACTAAAAAGCGTCATTTTAAACATTAA

Upstream 100 bases:

>100_bases
ATAGTAATCTATTTTTAAAAAATAAAAATTTTTACATATAAAAAATATTAAAATTTATATTAATTCATAGTCAATATTTT
TATAAAAAAGAAAATAAATT

Downstream 100 bases:

>100_bases
TAAAAGAGTAAAATTTATACAAATTTTTATTTTTTTAAATTATTAATATGGCTTATTCTTTAAATAAAGATATTATTTTT
TATCTAAAAAATTAAATTTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSLNNIIKNALVSLSDKTDLLKISKILAEKKINLFCTGGTADVLKKNKIPVLEISDYTNFPEIMNGRLKTLHPKIIGGIL
GRREKDQKIMKLHNLIPIDIVIVNFYPFEKIQNRKEFTIEEIIDNIDIGGPTLVRAAAKNYKDVIVIVDLSDFTSCIQLI
NTNTVSLETRFDLATKAFKYTALYEEIISKYFLKKNPYRKKHQKNIFPNEFQLNFIKKQDLRYGENQHQKSSFYIEKEIL
KSGTISSSNQIQGKNLSYNNICDADIALECVKEFSKPTCVIVKHGNPCGVSESNSLIKAYFSAYNADPISAFGGIIAFNC
LLDLDTAQEIVKKQFVEVIIAPEIDEMAVKILKRKKNIRLLICGKIEKNKKGLDFKRITNGLLIQEYDCDEINAKNFDFV
TNRLPTEKELEDAIFSWKVAKFVKSNAIVYSLNKTTIGIGAGQTSRIDATKLANLKVKDRNHNNTTGATMASDAFFPFRD
GIDNAALIGISCIIQPGGSIRDKEVIESANEHNISMIFTKKRHFKH

Sequences:

>Translated_526_residues
MSLNNIIKNALVSLSDKTDLLKISKILAEKKINLFCTGGTADVLKKNKIPVLEISDYTNFPEIMNGRLKTLHPKIIGGIL
GRREKDQKIMKLHNLIPIDIVIVNFYPFEKIQNRKEFTIEEIIDNIDIGGPTLVRAAAKNYKDVIVIVDLSDFTSCIQLI
NTNTVSLETRFDLATKAFKYTALYEEIISKYFLKKNPYRKKHQKNIFPNEFQLNFIKKQDLRYGENQHQKSSFYIEKEIL
KSGTISSSNQIQGKNLSYNNICDADIALECVKEFSKPTCVIVKHGNPCGVSESNSLIKAYFSAYNADPISAFGGIIAFNC
LLDLDTAQEIVKKQFVEVIIAPEIDEMAVKILKRKKNIRLLICGKIEKNKKGLDFKRITNGLLIQEYDCDEINAKNFDFV
TNRLPTEKELEDAIFSWKVAKFVKSNAIVYSLNKTTIGIGAGQTSRIDATKLANLKVKDRNHNNTTGATMASDAFFPFRD
GIDNAALIGISCIIQPGGSIRDKEVIESANEHNISMIFTKKRHFKH
>Mature_525_residues
SLNNIIKNALVSLSDKTDLLKISKILAEKKINLFCTGGTADVLKKNKIPVLEISDYTNFPEIMNGRLKTLHPKIIGGILG
RREKDQKIMKLHNLIPIDIVIVNFYPFEKIQNRKEFTIEEIIDNIDIGGPTLVRAAAKNYKDVIVIVDLSDFTSCIQLIN
TNTVSLETRFDLATKAFKYTALYEEIISKYFLKKNPYRKKHQKNIFPNEFQLNFIKKQDLRYGENQHQKSSFYIEKEILK
SGTISSSNQIQGKNLSYNNICDADIALECVKEFSKPTCVIVKHGNPCGVSESNSLIKAYFSAYNADPISAFGGIIAFNCL
LDLDTAQEIVKKQFVEVIIAPEIDEMAVKILKRKKNIRLLICGKIEKNKKGLDFKRITNGLLIQEYDCDEINAKNFDFVT
NRLPTEKELEDAIFSWKVAKFVKSNAIVYSLNKTTIGIGAGQTSRIDATKLANLKVKDRNHNNTTGATMASDAFFPFRDG
IDNAALIGISCIIQPGGSIRDKEVIESANEHNISMIFTKKRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=481, Percent_Identity=34.7193347193347, Blast_Score=225, Evalue=8e-59,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=53.6723163841808, Blast_Score=609, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI71985564, Length=482, Percent_Identity=33.402489626556, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI71985574, Length=317, Percent_Identity=28.7066246056782, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985556, Length=61, Percent_Identity=49.1803278688525, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323056, Length=499, Percent_Identity=31.6633266533066, Blast_Score=219, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6323768, Length=496, Percent_Identity=31.4516129032258, Blast_Score=219, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24649832, Length=494, Percent_Identity=32.5910931174089, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_BUCAP (Q8KA70)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660392.1
- ProteinModelPortal:   Q8KA70
- SMR:   Q8KA70
- EnsemblBacteria:   EBBUCT00000000333
- GeneID:   1005848
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg032
- GeneTree:   EBGT00050000008052
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   BAPH198804:BUSG032-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 59423; Mature: 59291

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNNIIKNALVSLSDKTDLLKISKILAEKKINLFCTGGTADVLKKNKIPVLEISDYTNF
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCCEEEECCCCCC
PEIMNGRLKTLHPKIIGGILGRREKDQKIMKLHNLIPIDIVIVNFYPFEKIQNRKEFTIE
HHHHCCCHHHCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCCCHH
EIIDNIDIGGPTLVRAAAKNYKDVIVIVDLSDFTSCIQLINTNTVSLETRFDLATKAFKY
HHHHCCCCCCHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
TALYEEIISKYFLKKNPYRKKHQKNIFPNEFQLNFIKKQDLRYGENQHQKSSFYIEKEIL
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCCCCHHEEEHHHH
KSGTISSSNQIQGKNLSYNNICDADIALECVKEFSKPTCVIVKHGNPCGVSESNSLIKAY
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
FSAYNADPISAFGGIIAFNCLLDLDTAQEIVKKQFVEVIIAPEIDEMAVKILKRKKNIRL
HHHCCCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCEEE
LICGKIEKNKKGLDFKRITNGLLIQEYDCDEINAKNFDFVTNRLPTEKELEDAIFSWKVA
EEEECCCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHH
KFVKSNAIVYSLNKTTIGIGAGQTSRIDATKLANLKVKDRNHNNTTGATMASDAFFPFRD
HHHHCCEEEEEECCEEEEECCCCCCCCCHHHEEEEEEECCCCCCCCCCEEECCCCCCHHC
GIDNAALIGISCIIQPGGSIRDKEVIESANEHNISMIFTKKRHFKH
CCCCCEEEEEEEEECCCCCCCHHHHHHCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SLNNIIKNALVSLSDKTDLLKISKILAEKKINLFCTGGTADVLKKNKIPVLEISDYTNF
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHCCCCCEEEECCCCCC
PEIMNGRLKTLHPKIIGGILGRREKDQKIMKLHNLIPIDIVIVNFYPFEKIQNRKEFTIE
HHHHCCCHHHCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCCCHH
EIIDNIDIGGPTLVRAAAKNYKDVIVIVDLSDFTSCIQLINTNTVSLETRFDLATKAFKY
HHHHCCCCCCHHHHHHHHCCCCEEEEEEECHHHHHHHHHHCCCEEEEEEHHHHHHHHHHH
TALYEEIISKYFLKKNPYRKKHQKNIFPNEFQLNFIKKQDLRYGENQHQKSSFYIEKEIL
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCCCCHHEEEHHHH
KSGTISSSNQIQGKNLSYNNICDADIALECVKEFSKPTCVIVKHGNPCGVSESNSLIKAY
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHH
FSAYNADPISAFGGIIAFNCLLDLDTAQEIVKKQFVEVIIAPEIDEMAVKILKRKKNIRL
HHHCCCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCEEE
LICGKIEKNKKGLDFKRITNGLLIQEYDCDEINAKNFDFVTNRLPTEKELEDAIFSWKVA
EEEECCCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHH
KFVKSNAIVYSLNKTTIGIGAGQTSRIDATKLANLKVKDRNHNNTTGATMASDAFFPFRD
HHHHCCEEEEEECCEEEEECCCCCCCCCHHHEEEEEEECCCCCCCCCCEEECCCCCCHHC
GIDNAALIGISCIIQPGGSIRDKEVIESANEHNISMIFTKKRHFKH
CCCCCEEEEEEEEECCCCCCCHHHHHHCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438