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The map label for this gene is metR

Identifier: 21672323

GI number: 21672323

Start: 30453

End: 31391

Strand: Reverse

Name: metR

Synonym: BUsg030

Alternate gene names: 21672323

Gene position: 31391-30453 (Counterclockwise)

Preceding gene: 21672325

Following gene: 21672321

Centisome position: 4.89

GC content: 27.58

Gene sequence:

>939_bases
ATGATCGAAATAAAACATCTTCGAACACTGCAAGCATTAAAAAACAATGGTTCATTAAGTGCGGCTGCAATTCAATTGCA
TCAAACACAATCAGCAATATCTCATCAATTTAATGAGTTAGAAAAAAAATTAGGTTTTAAATTATTTATTAGAAAAAGCA
ACCCTATAAAGTTTACAGCACAAGGTGAAATTTTACTGCAATTATCAAAAGAAATACTACCAAAAATTCATAAAGCAATA
CAAAATTGTAAAAAAACTCATCAGATGATTATTAAGCTCGCGATTGAATGTCATAGTTGTATTCAATGGTTAACACCAGC
ATTAAAAATTTTTCAAAAAAAATGGCCAAGAGTAGAAATAGATTTTTATTCAGATATGATTTTTAGTCCTCAACCTTCTC
TTCAACAAGGAAAATTAGACATTGTATTAACTTCAGAAGTTTTACCAAGAAGCAATTTGTTTTATTTTCCTATATTTGAT
TTTGAAGTTCGTTTAGTATTATCTCCTAATCATCCTTTAGCTCAAAAAAAACATAATATCCTTCCAGAAGATTTAATTTC
TGAAATACTTATGATATATCCTGTTAAACGTGATAAATTAGATATATGGAAATTTTTTTTACAACCAGCTGGAATCATAC
CAATCTTTAAAAATGTAAATAACACTTTACTTTTAATTCAAATGGTTGCAGCAAAAATGGGCATTACTGCATTACCACAT
TGGGTAGTAGACACTTTTGAAAAACAAGGTTTAGTAGTGACAAAAAAATTAGGAGATGGAATATGGAAACGTTTATATGC
TGCTATACGTGACGGTGATCAAAAAGAACTAATAATAAAAAATTTTATTTATGCTATACGTTTACATGTTTGTACTCATT
TAAAATTCATTCGTGATACACAAAAACCGCATTTTTTTAAAAACGTTAAAAACATTTAA

Upstream 100 bases:

>100_bases
AATTTTATATATTGATTTATTTAAATCAGATATCTTATAATTCTCATATCCAGTAAAATTCGCAAGCGCAAATAGTTAAT
TCTCGATATGAAGGAATCTC

Downstream 100 bases:

>100_bases
TAAATTTATTATTTTTTATAAAATTTTTATTTAAAAAAACTGCAACACCATCATTTTTATTGCTTCCAATAACCTCAGCA
TATGGCAAATTCTTTTTTAA

Product: transcriptional activator protein MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIEIKHLRTLQALKNNGSLSAAAIQLHQTQSAISHQFNELEKKLGFKLFIRKSNPIKFTAQGEILLQLSKEILPKIHKAI
QNCKKTHQMIIKLAIECHSCIQWLTPALKIFQKKWPRVEIDFYSDMIFSPQPSLQQGKLDIVLTSEVLPRSNLFYFPIFD
FEVRLVLSPNHPLAQKKHNILPEDLISEILMIYPVKRDKLDIWKFFLQPAGIIPIFKNVNNTLLLIQMVAAKMGITALPH
WVVDTFEKQGLVVTKKLGDGIWKRLYAAIRDGDQKELIIKNFIYAIRLHVCTHLKFIRDTQKPHFFKNVKNI

Sequences:

>Translated_312_residues
MIEIKHLRTLQALKNNGSLSAAAIQLHQTQSAISHQFNELEKKLGFKLFIRKSNPIKFTAQGEILLQLSKEILPKIHKAI
QNCKKTHQMIIKLAIECHSCIQWLTPALKIFQKKWPRVEIDFYSDMIFSPQPSLQQGKLDIVLTSEVLPRSNLFYFPIFD
FEVRLVLSPNHPLAQKKHNILPEDLISEILMIYPVKRDKLDIWKFFLQPAGIIPIFKNVNNTLLLIQMVAAKMGITALPH
WVVDTFEKQGLVVTKKLGDGIWKRLYAAIRDGDQKELIIKNFIYAIRLHVCTHLKFIRDTQKPHFFKNVKNI
>Mature_312_residues
MIEIKHLRTLQALKNNGSLSAAAIQLHQTQSAISHQFNELEKKLGFKLFIRKSNPIKFTAQGEILLQLSKEILPKIHKAI
QNCKKTHQMIIKLAIECHSCIQWLTPALKIFQKKWPRVEIDFYSDMIFSPQPSLQQGKLDIVLTSEVLPRSNLFYFPIFD
FEVRLVLSPNHPLAQKKHNILPEDLISEILMIYPVKRDKLDIWKFFLQPAGIIPIFKNVNNTLLLIQMVAAKMGITALPH
WVVDTFEKQGLVVTKKLGDGIWKRLYAAIRDGDQKELIIKNFIYAIRLHVCTHLKFIRDTQKPHFFKNVKNI

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1790262, Length=305, Percent_Identity=62.9508196721312, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=24.0677966101695, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082024, Length=173, Percent_Identity=27.7456647398844, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787530, Length=253, Percent_Identity=24.5059288537549, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786401, Length=244, Percent_Identity=23.7704918032787, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789506, Length=169, Percent_Identity=24.8520710059172, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=25.7028112449799, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788706, Length=171, Percent_Identity=24.5614035087719, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI157672245, Length=242, Percent_Identity=22.7272727272727, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METR_BUCAP (Q8KA72)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660390.1
- ProteinModelPortal:   Q8KA72
- EnsemblBacteria:   EBBUCT00000000072
- GeneID:   1005846
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg030
- HOGENOM:   HBG685425
- OMA:   ISEILMI
- BioCyc:   BAPH198804:BUSG030-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 36187; Mature: 36187

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKHLRTLQALKNNGSLSAAAIQLHQTQSAISHQFNELEKKLGFKLFIRKSNPIKFTA
CCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
QGEILLQLSKEILPKIHKAIQNCKKTHQMIIKLAIECHSCIQWLTPALKIFQKKWPRVEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DFYSDMIFSPQPSLQQGKLDIVLTSEVLPRSNLFYFPIFDFEVRLVLSPNHPLAQKKHNI
EEHHCEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEECEEEEEEECCCCCHHHHHCCC
LPEDLISEILMIYPVKRDKLDIWKFFLQPAGIIPIFKNVNNTLLLIQMVAAKMGITALPH
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCHHCCH
WVVDTFEKQGLVVTKKLGDGIWKRLYAAIRDGDQKELIIKNFIYAIRLHVCTHLKFIRDT
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QKPHFFKNVKNI
CCCHHHHHHCCC
>Mature Secondary Structure
MIEIKHLRTLQALKNNGSLSAAAIQLHQTQSAISHQFNELEKKLGFKLFIRKSNPIKFTA
CCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
QGEILLQLSKEILPKIHKAIQNCKKTHQMIIKLAIECHSCIQWLTPALKIFQKKWPRVEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
DFYSDMIFSPQPSLQQGKLDIVLTSEVLPRSNLFYFPIFDFEVRLVLSPNHPLAQKKHNI
EEHHCEEECCCCCCCCCCEEEEEECCCCCCCCEEEEEEECEEEEEEECCCCCHHHHHCCC
LPEDLISEILMIYPVKRDKLDIWKFFLQPAGIIPIFKNVNNTLLLIQMVAAKMGITALPH
CHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCHHCCH
WVVDTFEKQGLVVTKKLGDGIWKRLYAAIRDGDQKELIIKNFIYAIRLHVCTHLKFIRDT
HHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
QKPHFFKNVKNI
CCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438