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The map label for this gene is rnhB

Identifier: 30263840

GI number: 30263840

Start: 3653759

End: 3654532

Strand: Reverse

Name: rnhB

Synonym: BA_3975

Alternate gene names: 30263840

Gene position: 3654532-3653759 (Counterclockwise)

Preceding gene: 30263841

Following gene: 30263839

Centisome position: 69.91

GC content: 36.69

Gene sequence:

>774_bases
ATGCAAAAAGTGACGATTCAAGAAGCGGAACATTTACTGCAAGAAATTATAAGTGAAGAGGACGATCGCTTTCAAATATT
AATAAAAGATGAACGAAAAGGGGTACAAAAACTCATTTCGAAGTGGTATAAACAAAAAGAGTTAGCACAAAAGGAAAAAG
AAAAATTTCTAGAAATGTCTAAGTATGAAAATGCGTTACGTGAAAAAGGCCTTACATATATCGCAGGTATAGATGAAGTA
GGGCGTGGACCGCTAGCTGGACCTGTTGTGACGGCAGCTGTTATCCTTCCAGAAGATTTTTATATTCCAGGGTTAAATGA
TTCGAAAAAGCTGAGTGAGGCGAAGCGAGAGCGTTTTTATGGTGAAATTAAAGCAAAAGCAATCGCGATTGGAGTTGGGA
TTGTATCACCGCAAGTTATTGATGAGATAAATATTTATCAAGCGACGAAACAAGCGATGTTAGATGCTATTGCGAATTTA
TCTTGTACACCAGAATATTTATTAATTGATGCGATGAAACTTCCTGCACCAATTCCGCAAACATCCATCATTAAAGGTGA
TGCGAAAAGTATTTCCATTTCAGCTGCATCCATTATTGCGAAAGTAACGAGAGATCGTATGATGAAAGAGCTTGGAGAAA
AGTATCCAGCATATGGATTTGAACAACATATGGGATATGGTACGAAACAACATTTAGAAGCGATTGAAGCACATGGAGTA
TTAGAAGAACATCGGAAATCGTTTGCGCCAATTAAAGATATGATTAAAAAATAA

Upstream 100 bases:

>100_bases
AGAGTTTGCAGAGCAGACAGAAGATGCAGAAAAAGTAGAAGAAGTATAAGACGTGCGTTTGTACGTCTTTTCTTTTTTGT
ATATAAAGGGAGTGGCATGA

Downstream 100 bases:

>100_bases
GCTGCGAAAACGCAGCTTATTTTTTATTTTCACATATAAACATTACTATTTTCAAAAAAGAATAAAGCGATTTCAGTTTT
CTGACACTTTATTGTAGACA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQKVTIQEAEHLLQEIISEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMSKYENALREKGLTYIAGIDEV
GRGPLAGPVVTAAVILPEDFYIPGLNDSKKLSEAKRERFYGEIKAKAIAIGVGIVSPQVIDEINIYQATKQAMLDAIANL
SCTPEYLLIDAMKLPAPIPQTSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGV
LEEHRKSFAPIKDMIKK

Sequences:

>Translated_257_residues
MQKVTIQEAEHLLQEIISEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMSKYENALREKGLTYIAGIDEV
GRGPLAGPVVTAAVILPEDFYIPGLNDSKKLSEAKRERFYGEIKAKAIAIGVGIVSPQVIDEINIYQATKQAMLDAIANL
SCTPEYLLIDAMKLPAPIPQTSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGV
LEEHRKSFAPIKDMIKK
>Mature_257_residues
MQKVTIQEAEHLLQEIISEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMSKYENALREKGLTYIAGIDEV
GRGPLAGPVVTAAVILPEDFYIPGLNDSKKLSEAKRERFYGEIKAKAIAIGVGIVSPQVIDEINIYQATKQAMLDAIANL
SCTPEYLLIDAMKLPAPIPQTSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGV
LEEHRKSFAPIKDMIKK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=54.4444444444444, Blast_Score=187, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17536263, Length=183, Percent_Identity=32.2404371584699, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_BACAA (C3P5N8)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868074.1
- ProteinModelPortal:   C3P5N8
- SMR:   C3P5N8
- EnsemblBacteria:   EBBACT00000128175
- GeneID:   7850854
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_3998
- GeneTree:   EBGT00050000002677
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28875; Mature: 28875

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVTIQEAEHLLQEIISEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMS
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYENALREKGLTYIAGIDEVGRGPLAGPVVTAAVILPEDFYIPGLNDSKKLSEAKRERFY
HHHHHHHHCCCEEEECCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
GEIKAKAIAIGVGIVSPQVIDEINIYQATKQAMLDAIANLSCTPEYLLIDAMKLPAPIPQ
HHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
TSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGV
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
LEEHRKSFAPIKDMIKK
HHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MQKVTIQEAEHLLQEIISEEDDRFQILIKDERKGVQKLISKWYKQKELAQKEKEKFLEMS
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYENALREKGLTYIAGIDEVGRGPLAGPVVTAAVILPEDFYIPGLNDSKKLSEAKRERFY
HHHHHHHHCCCEEEECCHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
GEIKAKAIAIGVGIVSPQVIDEINIYQATKQAMLDAIANLSCTPEYLLIDAMKLPAPIPQ
HHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
TSIIKGDAKSISISAASIIAKVTRDRMMKELGEKYPAYGFEQHMGYGTKQHLEAIEAHGV
CCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
LEEHRKSFAPIKDMIKK
HHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA