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The map label for this gene is spaK [H]

Identifier: 30263769

GI number: 30263769

Start: 3570711

End: 3572072

Strand: Reverse

Name: spaK [H]

Synonym: BA_3899

Alternate gene names: 30263769

Gene position: 3572072-3570711 (Counterclockwise)

Preceding gene: 30263770

Following gene: 30263768

Centisome position: 68.34

GC content: 32.45

Gene sequence:

>1362_bases
ATGGAGAAAGTGAAAAAGCAAGTCAATCTTAGTAAGATTTTAATACAGTATATTTTTGCATTTTCAATAGGGACGATTGC
ATTAGTTACTTTTTTTATGATTCTATTCCAAGTTGGAGTTAACGCAGAGATAATCCTACCAGCAAATTATTATGAAAAAC
AGATTGAGTCGCAAAGAGATGCTATAGTTAAAGCGGAACAGGAAGATGATCAAATTTTAAAACAATATAAATATGCTCTG
TATGATTTTTCAGGAAACGTTATACAAGGGAGTATTAATAAAGATTATGCAGGACAAATATGGAATAACATACATAATGA
TGAAAAAGGTGACAGTAAGTACTACTATGCAATTATAGAACGCGATAAAGATATTTGTGTCATTCTATATACGTTACACG
CTACTTTTAAAAATCCTATTTTGCAGAAGTATGTACCGAGTCCGGAAGTTTGTGAAATTGTTCTTTTTATTCTACTATTT
GTTGCCGAAGTGATATTTCTATCTCGCTCTTTTGGTAAATCATTGTCAAAGGAAATGATGATTTTGAGAAAGGTAACAGA
TAAAATTCAAAAGGAAGATTTAGATTTTCAAGTGGAACAATCTAAAATAAAAGAAGTGAATGAAGTATTAACTTCTTTAG
TAAAAATGAAAGATGAATTAAATCATTCGTTACGTACACAGTGGAATATGGAAACAAATAGAAGAGAGCAGATTGCTGCT
TTGGCACATGACATTAAAACACCGTTAACGATATTGAGAGGGAATGCAGAGCTATTAAATGAATCAATGCTAGATTCAAA
TCAGAAGAAATGTAATGAGCATATTTTGAAAAGTATTGATGAGATGGAAATATATATGAAATCTTTACTCGATATTACGA
AATGTGAGGAAGGCGCTCCACTTCAATTCATGCAAACAGATCTCCGAGACTTTATTCATAATATTATAGAAGAAAGTGCA
ATTTGTGTTTTAGGCAAACAGTTAAATCTAATTGAAAATGTTCAGGACATACCAAATTTTATTCCTATTGACGAGACCGC
TTTGAAAAGAGCAATTATGAATATAATGATGAATGCAATTGAATATTCTCCAACAAAGGGTGACTTGATGTTCTCAGTGG
AAATGATTAGTGAAAAACTGCAATTTATTGTAGAGGACTCGGGAAGAGGGTTTACAGAAGAGGAAATGCATTCAGCTACA
GAGCAGTTTTATAGGGGAGATAAAAGTAGAAACGCCAAAGATCATCATGGAATGGGGTTATACATTGCTAAAAGCTTTGC
AAAGCAACACGGTGGAAACCTTTACTTAAGTAATTCGGAAAAGCTGCATGGTGCAAAAGTTGTTTTGGAAATATCAGCGT
AG

Upstream 100 bases:

>100_bases
GTGATAACCAAGTAATTGTAGAACATATTAAAAATATCCGTCATAAATTATCTAAGTATGGCATGAATCCAATAGAGACT
GTATGGGGAATTGGTTATAA

Downstream 100 bases:

>100_bases
TAGAGAGGGGGCAGATTATAACAATTCGCTCCTTTTTAATATAATTCTTTTACAACTATATGTTAAAAATTCATTTATAA
ACTAGAGATAACCTCAACCT

Product: lantibiotic biosynthesis sensor histidine kinase, putative

Products: NA

Alternate protein names: Subtilin biosynthesis sensor protein spaK [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MEKVKKQVNLSKILIQYIFAFSIGTIALVTFFMILFQVGVNAEIILPANYYEKQIESQRDAIVKAEQEDDQILKQYKYAL
YDFSGNVIQGSINKDYAGQIWNNIHNDEKGDSKYYYAIIERDKDICVILYTLHATFKNPILQKYVPSPEVCEIVLFILLF
VAEVIFLSRSFGKSLSKEMMILRKVTDKIQKEDLDFQVEQSKIKEVNEVLTSLVKMKDELNHSLRTQWNMETNRREQIAA
LAHDIKTPLTILRGNAELLNESMLDSNQKKCNEHILKSIDEMEIYMKSLLDITKCEEGAPLQFMQTDLRDFIHNIIEESA
ICVLGKQLNLIENVQDIPNFIPIDETALKRAIMNIMMNAIEYSPTKGDLMFSVEMISEKLQFIVEDSGRGFTEEEMHSAT
EQFYRGDKSRNAKDHHGMGLYIAKSFAKQHGGNLYLSNSEKLHGAKVVLEISA

Sequences:

>Translated_453_residues
MEKVKKQVNLSKILIQYIFAFSIGTIALVTFFMILFQVGVNAEIILPANYYEKQIESQRDAIVKAEQEDDQILKQYKYAL
YDFSGNVIQGSINKDYAGQIWNNIHNDEKGDSKYYYAIIERDKDICVILYTLHATFKNPILQKYVPSPEVCEIVLFILLF
VAEVIFLSRSFGKSLSKEMMILRKVTDKIQKEDLDFQVEQSKIKEVNEVLTSLVKMKDELNHSLRTQWNMETNRREQIAA
LAHDIKTPLTILRGNAELLNESMLDSNQKKCNEHILKSIDEMEIYMKSLLDITKCEEGAPLQFMQTDLRDFIHNIIEESA
ICVLGKQLNLIENVQDIPNFIPIDETALKRAIMNIMMNAIEYSPTKGDLMFSVEMISEKLQFIVEDSGRGFTEEEMHSAT
EQFYRGDKSRNAKDHHGMGLYIAKSFAKQHGGNLYLSNSEKLHGAKVVLEISA
>Mature_453_residues
MEKVKKQVNLSKILIQYIFAFSIGTIALVTFFMILFQVGVNAEIILPANYYEKQIESQRDAIVKAEQEDDQILKQYKYAL
YDFSGNVIQGSINKDYAGQIWNNIHNDEKGDSKYYYAIIERDKDICVILYTLHATFKNPILQKYVPSPEVCEIVLFILLF
VAEVIFLSRSFGKSLSKEMMILRKVTDKIQKEDLDFQVEQSKIKEVNEVLTSLVKMKDELNHSLRTQWNMETNRREQIAA
LAHDIKTPLTILRGNAELLNESMLDSNQKKCNEHILKSIDEMEIYMKSLLDITKCEEGAPLQFMQTDLRDFIHNIIEESA
ICVLGKQLNLIENVQDIPNFIPIDETALKRAIMNIMMNAIEYSPTKGDLMFSVEMISEKLQFIVEDSGRGFTEEEMHSAT
EQFYRGDKSRNAKDHHGMGLYIAKSFAKQHGGNLYLSNSEKLHGAKVVLEISA

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaK may function as a membrane-associated protein kinase that phosphorylates spaR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=207, Percent_Identity=29.4685990338164, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=26.5486725663717, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786783, Length=334, Percent_Identity=22.4550898203593, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=199, Percent_Identity=24.6231155778894, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=25.7383966244726, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789808, Length=204, Percent_Identity=25.4901960784314, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=25.5605381165919, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=23.0769230769231, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=289, Percent_Identity=22.1453287197232, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1788549, Length=267, Percent_Identity=23.5955056179775, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR008358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52102; Mature: 52102

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVKKQVNLSKILIQYIFAFSIGTIALVTFFMILFQVGVNAEIILPANYYEKQIESQRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
AIVKAEQEDDQILKQYKYALYDFSGNVIQGSINKDYAGQIWNNIHNDEKGDSKYYYAIIE
HHHCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEE
RDKDICVILYTLHATFKNPILQKYVPSPEVCEIVLFILLFVAEVIFLSRSFGKSLSKEMM
CCCCEEEEEEEHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRKVTDKIQKEDLDFQVEQSKIKEVNEVLTSLVKMKDELNHSLRTQWNMETNRREQIAA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHH
LAHDIKTPLTILRGNAELLNESMLDSNQKKCNEHILKSIDEMEIYMKSLLDITKCEEGAP
HHHHHHCCHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LQFMQTDLRDFIHNIIEESAICVLGKQLNLIENVQDIPNFIPIDETALKRAIMNIMMNAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EYSPTKGDLMFSVEMISEKLQFIVEDSGRGFTEEEMHSATEQFYRGDKSRNAKDHHGMGL
HCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
YIAKSFAKQHGGNLYLSNSEKLHGAKVVLEISA
HHHHHHHHHCCCEEEECCCCCCCCEEEEEEECC
>Mature Secondary Structure
MEKVKKQVNLSKILIQYIFAFSIGTIALVTFFMILFQVGVNAEIILPANYYEKQIESQRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHH
AIVKAEQEDDQILKQYKYALYDFSGNVIQGSINKDYAGQIWNNIHNDEKGDSKYYYAIIE
HHHCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEE
RDKDICVILYTLHATFKNPILQKYVPSPEVCEIVLFILLFVAEVIFLSRSFGKSLSKEMM
CCCCEEEEEEEHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRKVTDKIQKEDLDFQVEQSKIKEVNEVLTSLVKMKDELNHSLRTQWNMETNRREQIAA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHH
LAHDIKTPLTILRGNAELLNESMLDSNQKKCNEHILKSIDEMEIYMKSLLDITKCEEGAP
HHHHHHCCHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LQFMQTDLRDFIHNIIEESAICVLGKQLNLIENVQDIPNFIPIDETALKRAIMNIMMNAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
EYSPTKGDLMFSVEMISEKLQFIVEDSGRGFTEEEMHSATEQFYRGDKSRNAKDHHGMGL
HCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
YIAKSFAKQHGGNLYLSNSEKLHGAKVVLEISA
HHHHHHHHHCCCEEEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8439156; 8085823 [H]