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The map label for this gene is spaR [H]

Identifier: 30263770

GI number: 30263770

Start: 3572060

End: 3572722

Strand: Reverse

Name: spaR [H]

Synonym: BA_3900

Alternate gene names: 30263770

Gene position: 3572722-3572060 (Counterclockwise)

Preceding gene: 30263771

Following gene: 30263769

Centisome position: 68.35

GC content: 34.39

Gene sequence:

>663_bases
ATGGCAAGAATATTAGCAATTGATGATGAAGACGGCATCCTTTACATTATAAAAAGTGCTCTAGAGAAAGAAGGTCATGA
GGTAACAGCTGTTAGTAATCCTCTTACTATACCAAAGGAGAAATTTTGTCATTATGATTTAATTCTATTAGATGTCATGA
TGCCAGAAATAGATGGTTTTACACTTTGTAAGGAAATACGTGATTTCGTTGATAGTCCAATTGTATTTCTAACAGCCAAA
ACAATGGAGAAGGATCTAATCACAGGGCTTAGTCTCGGTGGAGATGACTATATTACAAAACCTTTTGGAATTGGAGAACT
GCGTGCGAGGGTATCCGCGCATGTAAGGCGTGAGCATAGAGAAAAGCAAAATGCATTTTTCCTGTCAGGTCTTACATTTC
ATCTGTCATGTAAAGAAATATGGTATGACGATACATCTATTGCATTTACGAAAAGTGAGTATGGAATTTGTGAATATTTA
GCTTTAAATCATGGACAAGTATTTTCAAGAGAAAAGATTTACGAAAATGTTTTTGGATATGAGGGAGAAAGTGATAACCA
AGTAATTGTAGAACATATTAAAAATATCCGTCATAAATTATCTAAGTATGGCATGAATCCAATAGAGACTGTATGGGGAA
TTGGTTATAAATGGAGAAAGTGA

Upstream 100 bases:

>100_bases
TTGCATTCGTTATTTCTCTTATGTGGTTTTCCTTTTATGAAGGACGCAAAGAAATGTAAGTGTAATTGTATTATAATGGA
AGAGTGGGGAGGGATTCTAT

Downstream 100 bases:

>100_bases
AAAAGCAAGTCAATCTTAGTAAGATTTTAATACAGTATATTTTTGCATTTTCAATAGGGACGATTGCATTAGTTACTTTT
TTTATGATTCTATTCCAAGT

Product: lantibiotic biosynthesis DNA-binding response regulator, putative

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MARILAIDDEDGILYIIKSALEKEGHEVTAVSNPLTIPKEKFCHYDLILLDVMMPEIDGFTLCKEIRDFVDSPIVFLTAK
TMEKDLITGLSLGGDDYITKPFGIGELRARVSAHVRREHREKQNAFFLSGLTFHLSCKEIWYDDTSIAFTKSEYGICEYL
ALNHGQVFSREKIYENVFGYEGESDNQVIVEHIKNIRHKLSKYGMNPIETVWGIGYKWRK

Sequences:

>Translated_220_residues
MARILAIDDEDGILYIIKSALEKEGHEVTAVSNPLTIPKEKFCHYDLILLDVMMPEIDGFTLCKEIRDFVDSPIVFLTAK
TMEKDLITGLSLGGDDYITKPFGIGELRARVSAHVRREHREKQNAFFLSGLTFHLSCKEIWYDDTSIAFTKSEYGICEYL
ALNHGQVFSREKIYENVFGYEGESDNQVIVEHIKNIRHKLSKYGMNPIETVWGIGYKWRK
>Mature_219_residues
ARILAIDDEDGILYIIKSALEKEGHEVTAVSNPLTIPKEKFCHYDLILLDVMMPEIDGFTLCKEIRDFVDSPIVFLTAKT
MEKDLITGLSLGGDDYITKPFGIGELRARVSAHVRREHREKQNAFFLSGLTFHLSCKEIWYDDTSIAFTKSEYGICEYLA
LNHGQVFSREKIYENVFGYEGESDNQVIVEHIKNIRHKLSKYGMNPIETVWGIGYKWRK

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=35.1351351351351, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790552, Length=216, Percent_Identity=30.0925925925926, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=30.7017543859649, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790860, Length=219, Percent_Identity=31.5068493150685, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367329, Length=226, Percent_Identity=34.070796460177, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=29.2792792792793, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=28.6995515695067, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786784, Length=175, Percent_Identity=32.5714285714286, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=29.7297297297297, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1789402, Length=216, Percent_Identity=30.0925925925926, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=29.5964125560538, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=27.1929824561404, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787229, Length=207, Percent_Identity=27.536231884058, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=25.2136752136752, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788550, Length=163, Percent_Identity=30.0613496932515, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322000, Length=109, Percent_Identity=34.8623853211009, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25303; Mature: 25172

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILAIDDEDGILYIIKSALEKEGHEVTAVSNPLTIPKEKFCHYDLILLDVMMPEIDGF
CCEEEEEECCCCEEHEEHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCH
TLCKEIRDFVDSPIVFLTAKTMEKDLITGLSLGGDDYITKPFGIGELRARVSAHVRREHR
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKQNAFFLSGLTFHLSCKEIWYDDTSIAFTKSEYGICEYLALNHGQVFSREKIYENVFGY
HHHCEEEEECEEEEEEEHHHEECCCCEEEECCCCCHHHHEECCCCCHHHHHHHHHHHCCC
EGESDNQVIVEHIKNIRHKLSKYGMNPIETVWGIGYKWRK
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARILAIDDEDGILYIIKSALEKEGHEVTAVSNPLTIPKEKFCHYDLILLDVMMPEIDGF
CEEEEEECCCCEEHEEHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCH
TLCKEIRDFVDSPIVFLTAKTMEKDLITGLSLGGDDYITKPFGIGELRARVSAHVRREHR
HHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
EKQNAFFLSGLTFHLSCKEIWYDDTSIAFTKSEYGICEYLALNHGQVFSREKIYENVFGY
HHHCEEEEECEEEEEEEHHHEECCCCEEEECCCCCHHHHEECCCCCHHHHHHHHHHHCCC
EGESDNQVIVEHIKNIRHKLSKYGMNPIETVWGIGYKWRK
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]