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The map label for this gene is gidB

Identifier: 77748759

GI number: 77748759

Start: 4898671

End: 4899309

Strand: Reverse

Name: gidB

Synonym: XAC4165

Alternate gene names: 77748759

Gene position: 4899309-4898671 (Counterclockwise)

Preceding gene: 21244885

Following gene: 21244881

Centisome position: 94.66

GC content: 70.58

Gene sequence:

>639_bases
ATGAACGATGCCGCACTCGCCCCCGATGTCTCCGCCGCGCTCGAGCGCGGCCTGCAGGCCCAATCCCTGGATGCGGCCTT
CGCCGCACCGCTGCTGCGCTACCTGGCCTTGCTGGTGCGCTGGAACAAGACCTACAACCTGACCGCCGTGCGTGACCCGC
GCGAGATGGTCACCCGCCATCTGCTCGATTCGTTGGCCATGCAGCCCTACATCGCCAGCGGCGCCCTGGCCGACCTGGGC
ACCGGCCCCGGCCTGCCCGGCATCCCGCTCGCCATCACCCGCCCGCAGCTGCAGGTGACGCTGGTGGAAAGCAACGGCAA
GAAGGCGCGCTTCATGCGCGAGGCCTTGCGCCATCTGGAGCTGGGCAACGCGCGCGTGGCCGAATCCCGTGCCGAAGCGC
TGGCCGAGCCGGCCGCCTACGACCACCTCACCGCGCGCGCGCTGGACACGTTGGCCGGCATCATCGCCGTCGGCGGCCAC
CTGCTGCGGCCGGGCGGCAGTTTGTTGGCGATGAAGGGTGTGTACCCGCACGAGGAAATCGCCGCATTGCCGGCTGGCTG
GACCGTGGGCGAGGTGCACCCGTTGCAGGTGCCCGGCCTGGAGGGCGAGCGGCATCTGGTGGTGGTGCGTAAGGGCTGA

Upstream 100 bases:

>100_bases
GTGGCAGGGGAACGGCCTGCGGAGGCAACCACGCCATCTCCAGCCGCGATGCCGAGATCGGTCCGCGCATGCCCGCCAGC
CCAGCTGCGATAATCGCGCC

Downstream 100 bases:

>100_bases
GCCGTTCGTTCTCGAAGCGCCCTCACCCCAACCCTCTCCCGCGGGCGGGAGAGGGAGCGAGCAGCGCTCCCGACAAGCTC
AGCGCCTGCCTTCGCTGGAT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNDAALAPDVSAALERGLQAQSLDAAFAAPLLRYLALLVRWNKTYNLTAVRDPREMVTRHLLDSLAMQPYIASGALADLG
TGPGLPGIPLAITRPQLQVTLVESNGKKARFMREALRHLELGNARVAESRAEALAEPAAYDHLTARALDTLAGIIAVGGH
LLRPGGSLLAMKGVYPHEEIAALPAGWTVGEVHPLQVPGLEGERHLVVVRKG

Sequences:

>Translated_212_residues
MNDAALAPDVSAALERGLQAQSLDAAFAAPLLRYLALLVRWNKTYNLTAVRDPREMVTRHLLDSLAMQPYIASGALADLG
TGPGLPGIPLAITRPQLQVTLVESNGKKARFMREALRHLELGNARVAESRAEALAEPAAYDHLTARALDTLAGIIAVGGH
LLRPGGSLLAMKGVYPHEEIAALPAGWTVGEVHPLQVPGLEGERHLVVVRKG
>Mature_212_residues
MNDAALAPDVSAALERGLQAQSLDAAFAAPLLRYLALLVRWNKTYNLTAVRDPREMVTRHLLDSLAMQPYIASGALADLG
TGPGLPGIPLAITRPQLQVTLVESNGKKARFMREALRHLELGNARVAESRAEALAEPAAYDHLTARALDTLAGIIAVGGH
LLRPGGSLLAMKGVYPHEEIAALPAGWTVGEVHPLQVPGLEGERHLVVVRKG

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=180, Percent_Identity=45.5555555555556, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_XANAC (Q8PF23)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644464.2
- ProteinModelPortal:   Q8PF23
- SMR:   Q8PF23
- GeneID:   1158236
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC4165
- NMPDR:   fig|190486.1.peg.4108
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   XAXO190486:XAC4165-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22588; Mature: 22588

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAALAPDVSAALERGLQAQSLDAAFAAPLLRYLALLVRWNKTYNLTAVRDPREMVTRH
CCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
LLDSLAMQPYIASGALADLGTGPGLPGIPLAITRPQLQVTLVESNGKKARFMREALRHLE
HHHHHHCCCHHHCCCHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHC
LGNARVAESRAEALAEPAAYDHLTARALDTLAGIIAVGGHLLRPGGSLLAMKGVYPHEEI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCHHHH
AALPAGWTVGEVHPLQVPGLEGERHLVVVRKG
HHCCCCCCCCCCCEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MNDAALAPDVSAALERGLQAQSLDAAFAAPLLRYLALLVRWNKTYNLTAVRDPREMVTRH
CCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
LLDSLAMQPYIASGALADLGTGPGLPGIPLAITRPQLQVTLVESNGKKARFMREALRHLE
HHHHHHCCCHHHCCCHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHC
LGNARVAESRAEALAEPAAYDHLTARALDTLAGIIAVGGHLLRPGGSLLAMKGVYPHEEI
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCHHHH
AALPAGWTVGEVHPLQVPGLEGERHLVVVRKG
HHCCCCCCCCCCCEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217