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The map label for this gene is aspG [C]

Identifier: 77748696

GI number: 77748696

Start: 3630311

End: 3631375

Strand: Reverse

Name: aspG [C]

Synonym: XAC3092

Alternate gene names: 77748696

Gene position: 3631375-3630311 (Counterclockwise)

Preceding gene: 21243820

Following gene: 21243818

Centisome position: 70.16

GC content: 65.16

Gene sequence:

>1065_bases
ATGCTTCAACGCCGTCACTTCCTCAAGAGCGCCACGCTAGGCGCGATTGCGTCCGGGTTGGCCGGCTGGGGCGCCCGCGC
GCAAGCCGCGGCAGGCGCCACGCCAGGCATGCGGCCGATGGCGGTGCCGCGTGGCGCGGCGCGGGTGATCTCCACCTGGG
ATTTCGGCGTCGCCGCCAATCAGGAAGCCTGGAAGATTCTCAGCAGCGGCGGTATTGCGCTGGATGCGGTGGAGGCCGGC
GTGCGTGTGCCGGAAGCGGACCCGAACAATCCCACCGTCGGCCTGGGCGGTTATCCGGACCGCGATGGCCGGGTAACGCT
GGACGCCTGCATCATGGATCACCTGGGCAATTGTGGCGCGGTGGCATCGCTGGAAGACGTGGTGCACGCCATTTCGGTGG
CACGTGCGGTGATGGAGAAAACCCCGCACGTGATGTTGGTGGGCGACGGCGCGCGTCAATTCGCCTTCGAACAGGGTTTT
CCGAAAACCAAGCTGTTGACTCCAAGCTCGGAAGCGGCCTGGAAAGAGTGGTTGAAGAGCTCCAAGTATTCGCCCGAAGC
CAATGTCGAAAATCGCGCCTGGCGAGATGCCAAGTTGCCCGGCGGCAAGGACAATCACGACACCATCGGCATGCTGGCGC
TGGATACGCACGGCAATCTATCCGGTGCATGCACCACCAGCGGCATGGCGTGGAAGATGCACGGCCGCGTGGGCGACAGC
CCGATCATCGGCGCGGGCCTGTACGTGGACAACGACGTGGGCGGGGCGACCTCGACGGGTGTGGGCGAGGAGGTGATCCG
CAACGTCGGCAGTTTTGCGGTGGTGGAAATGATGCGGCAGGGCAAATCGCCCGCCGATGCCTGCCGCGAGGTGGTGATGC
GGCTGATCCGTCGCAAGCCGGAGCTGACGCGCACATTGCAGGTCGGCTTTCTGGCAATGAACAAGCGCGGCGAAGTGGGA
GCGTTCGCGATCCAGAAGGGCTTCAGTTACGCGGTGTGCGACGCGCAGCGGCAGGATCTGCTGGTGCCTGGCGAGAGCCA
TTACACCAGCGAGCCGGCAGCATGA

Upstream 100 bases:

>100_bases
ACAACGCCGCAATAAGGTTTATCCGCACGCCACGCTGTCGCATGATGGAAGGCTGGTACCGCACTGCGCGGCCGCTCGTT
TCCGTCATGGAGTCATCCGC

Downstream 100 bases:

>100_bases
GCGCGGTGGGATTGGAAGTGGCTGCCGATTCGGTGGCCTCGGCATTGGCCGCGCAAGCAGGCGGTGCGATGCGCGTGGAG
CTGTGCGGCGGGCTGGATGG

Product: asparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGMRPMAVPRGAARVISTWDFGVAANQEAWKILSSGGIALDAVEAG
VRVPEADPNNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGF
PKTKLLTPSSEAAWKEWLKSSKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNDVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA

Sequences:

>Translated_354_residues
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGMRPMAVPRGAARVISTWDFGVAANQEAWKILSSGGIALDAVEAG
VRVPEADPNNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGF
PKTKLLTPSSEAAWKEWLKSSKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNDVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA
>Mature_354_residues
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGMRPMAVPRGAARVISTWDFGVAANQEAWKILSSGGIALDAVEAG
VRVPEADPNNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGF
PKTKLLTPSSEAAWKEWLKSSKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNDVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=319, Percent_Identity=36.6771159874608, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI285002253, Length=319, Percent_Identity=34.7962382445141, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI145275202, Length=229, Percent_Identity=37.9912663755458, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI145275200, Length=229, Percent_Identity=37.9912663755458, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI98991769, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787050, Length=259, Percent_Identity=37.0656370656371, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17569239, Length=302, Percent_Identity=34.4370860927152, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI221330102, Length=301, Percent_Identity=39.8671096345515, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24652135, Length=301, Percent_Identity=39.8671096345515, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655553, Length=300, Percent_Identity=34, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24657358, Length=297, Percent_Identity=33.6700336700337, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18921183, Length=252, Percent_Identity=34.9206349206349, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 37453; Mature: 37453

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGMRPMAVPRGAARVISTWDFGVAAN
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCCCC
QEAWKILSSGGIALDAVEAGVRVPEADPNNPTVGLGGYPDRDGRVTLDACIMDHLGNCGA
HHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCEEECCCCCCCCCCEEHHHHHHHHCCCCCC
VASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGFPKTKLLTPSSEAAWKEWLKS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEECCCCCCC
PIIGAGLYVDNDVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKP
CEEEECEEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ELTRTLQVGFLAMNKRGEVGAFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA
HHHHHHHHEEEEECCCCCCCHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGMRPMAVPRGAARVISTWDFGVAAN
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCCCC
QEAWKILSSGGIALDAVEAGVRVPEADPNNPTVGLGGYPDRDGRVTLDACIMDHLGNCGA
HHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCEEECCCCCCCCCCEEHHHHHHHHCCCCCC
VASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGFPKTKLLTPSSEAAWKEWLKS
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEECCCCCCC
PIIGAGLYVDNDVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKP
CEEEECEEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ELTRTLQVGFLAMNKRGEVGAFAIQKGFSYAVCDAQRQDLLVPGESHYTSEPAA
HHHHHHHHEEEEECCCCCCCHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]