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The map label for this gene is hrpW [H]

Identifier: 77748681

GI number: 77748681

Start: 3428693

End: 3429598

Strand: Direct

Name: hrpW [H]

Synonym: XAC2922

Alternate gene names: 77748681

Gene position: 3428693-3429598 (Clockwise)

Preceding gene: 21243647

Following gene: 21243652

Centisome position: 66.25

GC content: 61.26

Gene sequence:

>906_bases
ATGTCGGAATTGCTACAGCGGTTGATACAGACCCAATTCCAACCTCAAAACACGTCGCAATACGATTGGAACCCTTCGCC
TTCCCATGGGACGTCCAATGGCCAAGGGGAGGGCAACGAGCATGGCGGTCGCAGGAACGGACGCGGCGGTGACAACTTCC
TCGCCACCACGCCCGAGACGTCCGACCATTCCGACAAATCCGCTCACTCGACCGTCACCGGCAGCGGCCCCGTGGGGAGC
GGTCGCACGATCAACGTCAACAGTCCGATCGTGGTTCACAAGGGCGAGGTGTTCGACGGACACAACAATCTCTACGTCGG
CGGGTCCGGCATCGGCGATGGTTCGCAGTCCGAGCACCAGCAACCGATGTTCGTCGTCGAACAAGGCGGCACCCTCCAAA
ACGTGCGGATGAGCGGGGGCGGCGACGGCATCCATCTGCTCGGCGACGCCACGCTGAAGAACGTCCACAACCTGAACGTC
AGCGAGGATGCGATGACCATCGACGGCCCTGGCAACCGGGAACATGACTCGCGCATTGCCGGAACGCCTGCCAGCGGCCT
GCCGGCGCGTCCAAAGATCGAAATCCTCGACAGTTCCTTCGACAATGCCTCCGACAAGGTGATCCAGGACAACCAGGCGG
CAGATGTGCTGTTGCGTAACGTCAGCGTGAACGGAGCCGGCAAGGTGTTCCGCACCAACGGCGGACACACCGACATCGAT
TCACACGTCACGGTCGAAGACTCCACCCTGAAAGGCATCAAGGAAGCAGTATTCCGCACCGATGCCCCCGGCGCACATGT
CAGTCTGCATGGCGTCCGCGACGATGCTCCCCATCAAGTGGAGGCACTGTCCCCCAGTCAGGCAGATGGCGCACGCAGCG
TCAGCCGAAAGGAATATACAGGTTGA

Upstream 100 bases:

>100_bases
GATGATTTCCTCAATCAACCCCTATATCAGTGCTAACCCACTCCTCAACCACACGCTTCAGACGAAGCAGCCCGCATTTT
CCGACACGATCATTATGCTG

Downstream 100 bases:

>100_bases
AAACACTGGCACGGCACGATGAGGACGGGGCGCCCGCTCACCCTTGGAGGGGAGCGGGCAACTGAGTCACCGGCGCGACC
GCGCGCGACTCAGCGCACTT

Product: HrpW protein

Products: NA

Alternate protein names: Pectin lyase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSELLQRLIQTQFQPQNTSQYDWNPSPSHGTSNGQGEGNEHGGRRNGRGGDNFLATTPETSDHSDKSAHSTVTGSGPVGS
GRTINVNSPIVVHKGEVFDGHNNLYVGGSGIGDGSQSEHQQPMFVVEQGGTLQNVRMSGGGDGIHLLGDATLKNVHNLNV
SEDAMTIDGPGNREHDSRIAGTPASGLPARPKIEILDSSFDNASDKVIQDNQAADVLLRNVSVNGAGKVFRTNGGHTDID
SHVTVEDSTLKGIKEAVFRTDAPGAHVSLHGVRDDAPHQVEALSPSQADGARSVSRKEYTG

Sequences:

>Translated_301_residues
MSELLQRLIQTQFQPQNTSQYDWNPSPSHGTSNGQGEGNEHGGRRNGRGGDNFLATTPETSDHSDKSAHSTVTGSGPVGS
GRTINVNSPIVVHKGEVFDGHNNLYVGGSGIGDGSQSEHQQPMFVVEQGGTLQNVRMSGGGDGIHLLGDATLKNVHNLNV
SEDAMTIDGPGNREHDSRIAGTPASGLPARPKIEILDSSFDNASDKVIQDNQAADVLLRNVSVNGAGKVFRTNGGHTDID
SHVTVEDSTLKGIKEAVFRTDAPGAHVSLHGVRDDAPHQVEALSPSQADGARSVSRKEYTG
>Mature_300_residues
SELLQRLIQTQFQPQNTSQYDWNPSPSHGTSNGQGEGNEHGGRRNGRGGDNFLATTPETSDHSDKSAHSTVTGSGPVGSG
RTINVNSPIVVHKGEVFDGHNNLYVGGSGIGDGSQSEHQQPMFVVEQGGTLQNVRMSGGGDGIHLLGDATLKNVHNLNVS
EDAMTIDGPGNREHDSRIAGTPASGLPARPKIEILDSSFDNASDKVIQDNQAADVLLRNVSVNGAGKVFRTNGGHTDIDS
HVTVEDSTLKGIKEAVFRTDAPGAHVSLHGVRDDAPHQVEALSPSQADGARSVSRKEYTG

Specific function: Catalyzes the depolymerization of both polygalacturonate and pectins of methyl esterification degree from 22 to 89%, with an endo mode of action. In contrast to the majority of pectate lyases, displays high activity on highly methylated pectins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004898
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF03211 Pectate_lyase [H]

EC number: =4.2.2.2; =4.2.2.10 [H]

Molecular weight: Translated: 31705; Mature: 31574

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLQRLIQTQFQPQNTSQYDWNPSPSHGTSNGQGEGNEHGGRRNGRGGDNFLATTPET
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
SDHSDKSAHSTVTGSGPVGSGRTINVNSPIVVHKGEVFDGHNNLYVGGSGIGDGSQSEHQ
CCCCCCCCCCEECCCCCCCCCCEEECCCCEEEECCCEEECCCCEEECCCCCCCCCCCCCC
QPMFVVEQGGTLQNVRMSGGGDGIHLLGDATLKNVHNLNVSEDAMTIDGPGNREHDSRIA
CCEEEEECCCCEEEEEECCCCCCEEEEECHHHHHHHCCCCCCCCEEECCCCCCCCCCEEC
GTPASGLPARPKIEILDSSFDNASDKVIQDNQAADVLLRNVSVNGAGKVFRTNGGHTDID
CCCCCCCCCCCEEEEECCCCCCCCCCEECCCCHHEEEEEEECCCCCCEEEEECCCCCCCC
SHVTVEDSTLKGIKEAVFRTDAPGAHVSLHGVRDDAPHQVEALSPSQADGARSVSRKEYT
CEEEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHCHHCCC
G
C
>Mature Secondary Structure 
SELLQRLIQTQFQPQNTSQYDWNPSPSHGTSNGQGEGNEHGGRRNGRGGDNFLATTPET
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
SDHSDKSAHSTVTGSGPVGSGRTINVNSPIVVHKGEVFDGHNNLYVGGSGIGDGSQSEHQ
CCCCCCCCCCEECCCCCCCCCCEEECCCCEEEECCCEEECCCCEEECCCCCCCCCCCCCC
QPMFVVEQGGTLQNVRMSGGGDGIHLLGDATLKNVHNLNVSEDAMTIDGPGNREHDSRIA
CCEEEEECCCCEEEEEECCCCCCEEEEECHHHHHHHCCCCCCCCEEECCCCCCCCCCEEC
GTPASGLPARPKIEILDSSFDNASDKVIQDNQAADVLLRNVSVNGAGKVFRTNGGHTDID
CCCCCCCCCCCEEEEECCCCCCCCCCEECCCCHHEEEEEEECCCCCCEEEEECCCCCCCC
SHVTVEDSTLKGIKEAVFRTDAPGAHVSLHGVRDDAPHQVEALSPSQADGARSVSRKEYT
CEEEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHCHHCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA