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The map label for this gene is tycC [H]

Identifier: 77748519

GI number: 77748519

Start: 285020

End: 286681

Strand: Direct

Name: tycC [H]

Synonym: XAC0237

Alternate gene names: 77748519

Gene position: 285020-286681 (Clockwise)

Preceding gene: 21241009

Following gene: 21241012

Centisome position: 5.51

GC content: 68.71

Gene sequence:

>1662_bases
ATGACGACCCTCTGCAATATCGCGGCCACCCTTCCGCAACTGGCGCGCGAGCGCCCCGACCAGATCGCCATCCGTTGCCC
GGGCGGTCGCGGCGCCAATGGGTTTGCCGCCTACGATGTGACCTTGAGCTATGCCGAGCTGGATGCGCGCAGCGATGCGA
TTGCCGCCGGCCTGGCACGCCACGGCATCGGTCGCGGCGCGCGTGCGGTGGTGATGGTGCGGCCGTCGCCGGAATTCTTC
CTGCTGATGTTCGCCTTGTTCAAGGCTGGCGCGGTACCGGTGCTGGTGGACCCCGGCATCGACAAACGTGCGCTCAAGCA
GTGTCTGGACGAAGCGCAGCCGCAGGCATTTATCGGCATTGCGCTGGCGCAGCTGGCGCGTCGCCTGTTGCGCTGGGCGC
GCTCGGCAACGCAGATCGTTACCGTCGGCGGACGTTACGGCTGGGGTGGAGTCACGCTGGCGCGGGTGGAGCGCGATGGC
GCCGGCGCAGGCAGCCAGCTGGCCGACACCGCGCCGGACGATGTGGCGGCGATCCTGTTCACCAGTGGCTCGACCGGTGT
GCCCAAGGGGGTGGTGTATCGCCACCGGCATTTCGTTGGCCAGATCGAATTGCTGCGCAACGCATTCGGCATGCAGCCCG
GCGGGGTGGATCTGCCGACGTTTCCGCCGTTCGCCTTGTTCGATCCGGCGCTGGGGCTGACCTCGGTGATTCCGGACATG
GACCCAACGCGCCCGGGCAGCGCCGATCCGCGTAAGTTGCACGACGCGATCAACCGCTTCGGCGTGACGCAGTTGTTCGG
TTCGCCCGCCTTGATGCGCGTGCTGGCCGACTATGGGCAGCCGCTTCCCAACGTGCGCCTGGCGACTTCCGCCGGTGCGC
CGGTGCCACCGGATGTGGTGGCGAAGATCCGCGCGCTGCTGCCGGCCGATGCGCAGTTCTGGACCCCGTACGGCGCCACC
GAGTGCCTGCCGGTGGCCGCGATCGAAGGTCGCACCCTGGACGCCACGCGCGCCGCCACCGAGGCTGGTGCCGGCACCTG
TGTGGGCCATGTCGTGCCGCCCAACCAGGTGCGCATCATCGCCATCGACGATGCGGCCATTGCCGACTGGAGCGGCGTGC
GCGAGCTGGCGACCGGTGAGGTCGGCGAGATCACCGTCGCCGGGCCCACCGCCACCGATACCTATTTCAACCGTGATGCG
GCCACGCGCATCGCCAAGATCCGCGAATGCTGCAGCGATGGCAGCGAGCGTGTCGTGCACCGCATGGGCGATGTCGGATA
TGTCGACGCCGAAGGGCGCTTGTGGTTCTGCGGGCGCAAGACCCACCGCGTGGAAGCCGCCAGCGGCCCGCTGTACACCG
AACAGGTGGAGCCGGTGTTCAACGTGCATCGGCAGGTGCGGCGCACCGCGCTGGTTGGCGTAGGTGCGCCCGGACAACAG
CAACCGGTGCTGTGCGTGGAGTTGCAGCCGGGGGTGTCTGCGTCGGCGTTCGCACAGGTGGAAAGCGACCTGCGCGCGCT
GGGCGCTGCCCACCCGCACACCGCAGGCGTCGCGCGTTTCCTGCGCCATTCCGGCTTTCCGGTCGACATCCGCCACAACG
CCAAGATTGGCCGCGAAACGCTGGCGGTCTGGGCTGCGCAGCAGCTGCATAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CGCCGCACGATGAGGAGCCGCGCTTGCGCCCGCGTCGCGCGTTGCCTTCAGGCAGCCTGCAAGCGGCCGGCGCCATCGGC
GCATGGGTGATAATGCGCCG

Downstream 100 bases:

>100_bases
CAACAGGTAGCGCGCAGTGGTCAGTCGGGCGTGGAGGGGGCACTCAGAACGGCAGTGAGCGGTACATGCCGATTCCGGCA
ACGTCCACGCCCGACTGACG

Product: peptide synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Tyrocidine synthase III; ATP-dependent asparagine adenylase; AsnA; Asparagine activase; ATP-dependent glutamine adenylase; GlnA; Glutamine activase; ATP-dependent tyrosine adenylase; TyrA; Tyrosine activase; ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent ornithine adenylase; OrnA; Ornithine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase [H]

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTTLCNIAATLPQLARERPDQIAIRCPGGRGANGFAAYDVTLSYAELDARSDAIAAGLARHGIGRGARAVVMVRPSPEFF
LLMFALFKAGAVPVLVDPGIDKRALKQCLDEAQPQAFIGIALAQLARRLLRWARSATQIVTVGGRYGWGGVTLARVERDG
AGAGSQLADTAPDDVAAILFTSGSTGVPKGVVYRHRHFVGQIELLRNAFGMQPGGVDLPTFPPFALFDPALGLTSVIPDM
DPTRPGSADPRKLHDAINRFGVTQLFGSPALMRVLADYGQPLPNVRLATSAGAPVPPDVVAKIRALLPADAQFWTPYGAT
ECLPVAAIEGRTLDATRAATEAGAGTCVGHVVPPNQVRIIAIDDAAIADWSGVRELATGEVGEITVAGPTATDTYFNRDA
ATRIAKIRECCSDGSERVVHRMGDVGYVDAEGRLWFCGRKTHRVEAASGPLYTEQVEPVFNVHRQVRRTALVGVGAPGQQ
QPVLCVELQPGVSASAFAQVESDLRALGAAHPHTAGVARFLRHSGFPVDIRHNAKIGRETLAVWAAQQLHKRG

Sequences:

>Translated_553_residues
MTTLCNIAATLPQLARERPDQIAIRCPGGRGANGFAAYDVTLSYAELDARSDAIAAGLARHGIGRGARAVVMVRPSPEFF
LLMFALFKAGAVPVLVDPGIDKRALKQCLDEAQPQAFIGIALAQLARRLLRWARSATQIVTVGGRYGWGGVTLARVERDG
AGAGSQLADTAPDDVAAILFTSGSTGVPKGVVYRHRHFVGQIELLRNAFGMQPGGVDLPTFPPFALFDPALGLTSVIPDM
DPTRPGSADPRKLHDAINRFGVTQLFGSPALMRVLADYGQPLPNVRLATSAGAPVPPDVVAKIRALLPADAQFWTPYGAT
ECLPVAAIEGRTLDATRAATEAGAGTCVGHVVPPNQVRIIAIDDAAIADWSGVRELATGEVGEITVAGPTATDTYFNRDA
ATRIAKIRECCSDGSERVVHRMGDVGYVDAEGRLWFCGRKTHRVEAASGPLYTEQVEPVFNVHRQVRRTALVGVGAPGQQ
QPVLCVELQPGVSASAFAQVESDLRALGAAHPHTAGVARFLRHSGFPVDIRHNAKIGRETLAVWAAQQLHKRG
>Mature_552_residues
TTLCNIAATLPQLARERPDQIAIRCPGGRGANGFAAYDVTLSYAELDARSDAIAAGLARHGIGRGARAVVMVRPSPEFFL
LMFALFKAGAVPVLVDPGIDKRALKQCLDEAQPQAFIGIALAQLARRLLRWARSATQIVTVGGRYGWGGVTLARVERDGA
GAGSQLADTAPDDVAAILFTSGSTGVPKGVVYRHRHFVGQIELLRNAFGMQPGGVDLPTFPPFALFDPALGLTSVIPDMD
PTRPGSADPRKLHDAINRFGVTQLFGSPALMRVLADYGQPLPNVRLATSAGAPVPPDVVAKIRALLPADAQFWTPYGATE
CLPVAAIEGRTLDATRAATEAGAGTCVGHVVPPNQVRIIAIDDAAIADWSGVRELATGEVGEITVAGPTATDTYFNRDAA
TRIAKIRECCSDGSERVVHRMGDVGYVDAEGRLWFCGRKTHRVEAASGPLYTEQVEPVFNVHRQVRRTALVGVGAPGQQQ
PVLCVELQPGVSASAFAQVESDLRALGAAHPHTAGVARFLRHSGFPVDIRHNAKIGRETLAVWAAQQLHKRG

Specific function: Incorporates six amino acids (for tyrocidine A, Asn, Gln, Tyr, Val, Orn, and Leu) in their L-configuration into the peptide product [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=503, Percent_Identity=22.6640159045726, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58682; Mature: 58551

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLCNIAATLPQLARERPDQIAIRCPGGRGANGFAAYDVTLSYAELDARSDAIAAGLAR
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHH
HGIGRGARAVVMVRPSPEFFLLMFALFKAGAVPVLVDPGIDKRALKQCLDEAQPQAFIGI
HCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHH
ALAQLARRLLRWARSATQIVTVGGRYGWGGVTLARVERDGAGAGSQLADTAPDDVAAILF
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEE
TSGSTGVPKGVVYRHRHFVGQIELLRNAFGMQPGGVDLPTFPPFALFDPALGLTSVIPDM
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCC
DPTRPGSADPRKLHDAINRFGVTQLFGSPALMRVLADYGQPLPNVRLATSAGAPVPPDVV
CCCCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
AKIRALLPADAQFWTPYGATECLPVAAIEGRTLDATRAATEAGAGTCVGHVVPPNQVRII
HHHHHHCCCCCCEECCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEECCCCCCCEEEE
AIDDAAIADWSGVRELATGEVGEITVAGPTATDTYFNRDAATRIAKIRECCSDGSERVVH
EECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
RMGDVGYVDAEGRLWFCGRKTHRVEAASGPLYTEQVEPVFNVHRQVRRTALVGVGAPGQQ
HCCCCCEEECCCCEEEECCCCCEEECCCCCCCHHHCCHHHHHHHHHHHHHEEECCCCCCC
QPVLCVELQPGVSASAFAQVESDLRALGAAHPHTAGVARFLRHSGFPVDIRHNAKIGRET
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHH
LAVWAAQQLHKRG
HHHHHHHHHHHCC
>Mature Secondary Structure 
TTLCNIAATLPQLARERPDQIAIRCPGGRGANGFAAYDVTLSYAELDARSDAIAAGLAR
CCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHH
HGIGRGARAVVMVRPSPEFFLLMFALFKAGAVPVLVDPGIDKRALKQCLDEAQPQAFIGI
HCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHH
ALAQLARRLLRWARSATQIVTVGGRYGWGGVTLARVERDGAGAGSQLADTAPDDVAAILF
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEE
TSGSTGVPKGVVYRHRHFVGQIELLRNAFGMQPGGVDLPTFPPFALFDPALGLTSVIPDM
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHCCCC
DPTRPGSADPRKLHDAINRFGVTQLFGSPALMRVLADYGQPLPNVRLATSAGAPVPPDVV
CCCCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHH
AKIRALLPADAQFWTPYGATECLPVAAIEGRTLDATRAATEAGAGTCVGHVVPPNQVRII
HHHHHHCCCCCCEECCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEECCCCCCCEEEE
AIDDAAIADWSGVRELATGEVGEITVAGPTATDTYFNRDAATRIAKIRECCSDGSERVVH
EECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
RMGDVGYVDAEGRLWFCGRKTHRVEAASGPLYTEQVEPVFNVHRQVRRTALVGVGAPGQQ
HCCCCCEEECCCCEEEECCCCCEEECCCCCCCHHHCCHHHHHHHHHHHHHEEECCCCCCC
QPVLCVELQPGVSASAFAQVESDLRALGAAHPHTAGVARFLRHSGFPVDIRHNAKIGRET
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHH
LAVWAAQQLHKRG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9352938; 10801488 [H]