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The map label for this gene is rffM [H]

Identifier: 77748501

GI number: 77748501

Start: 56424

End: 57854

Strand: Reverse

Name: rffM [H]

Synonym: XAC0046

Alternate gene names: 77748501

Gene position: 57854-56424 (Counterclockwise)

Preceding gene: 21240821

Following gene: 21240819

Centisome position: 1.12

GC content: 68.13

Gene sequence:

>1431_bases
TTGGCAGCAATACCTCTCACAGCGTCGGCTGCCGCCCAAGCGCGCCCTGCTGCTGCCCAATGCCGTGCTCGCCGCCGAGC
AGTGCCTGCCCGCGACGTCACGGCAACCGTTGCTCGGGGCTCCGGCGCTGCTGTCGGTCGGCCGCCTGGACGCGGTCAAG
CGGCTACAGGATGCGATCGCAGCCCTTACCCAGCCGAACATGGACGCAGCCGTGTTGCACGTGGTCGGACGTGGCCCGGA
TCGGGCACGCCTGGAAGCCTGCGCCGTGGCATTGGGGGTCGCTGCACGCGTGCACTTCCATGGCTTCGTCAGCGATGCCG
AGGTTGCAGCGATGGCGGCACAGGCCGATGTCTTCGTGCTGCCATCGGCCGTCGAGGGCATGCCGACCGTCTTGATCGAA
ATGCTGCTGCATGGGTGTCCGGTGGTTGCCAGCGACATTCCCGGCAATCGCTCGATCCTGGCCCAGATCGGCCTGCAGGC
GGCCTTGCACCGGCTCGGCGACAGCGCCGCGTTGGCGGGATGCATTCGCGTGCAACGACAGCAGCGAATTGGGCCGGAAC
GCATCGCGCGTGTCCGGGCAGATTTCACCTGGGAGGGCCTGCGAGCGCAGGTGCTGGCACTGTACGATGCTCAGGTCGTA
AAGGAACCCGCAACTTGACCACATCGCCGGCTCGCCTGTCCTTTCTCGGATGTCCGTTGGACCTCCTCCCCCCGGAGCAG
ATTCTCTTGCGTGCGCAGCACGCCGCCGAGGGCGGCGCGCCGATCCGGGTGGAGGGCTTGAACGTCGCCAAACTGATCGA
TGCGCGCGCCCAGCTGCGCCAGGCGCTGGAACAGGCCGAAGTGGTGCACATCGATGGCGCCGGCATCAGCCTGGGCGTGC
GTTGGTTCGGAGTGGACGTGCCGCCGCGCAGGGCCGGCATCGACCTGATGCAGGACATGTGCGGCCAGGCCGCCCGCAGC
GGTGCAGGGGTCTATCTGCTCGGTGCGCGCCCGGCGGTGGTCGCGGCCGCGGCAGAACGCCTGTGCACGCACTGGCCCGG
TCTGCACATTGCCGGCATGCGCGATGGCTATTTCAGCGACGCCGAGGAGGACGCGGTGATCGCGGCAATTCGCCAGTCGG
GTGCACGCTATCTCTTCATCGGCATCAGTTCGCCGAAGAAGGAACTGCTGCTGGAGAAACACTGGCAAACCCTGGGCGTG
CCGGTGGCGATGGGCGTGGGCGGTTCGTTCGATGTGCTGTCGGGCATGCTGCCGCGTGCGCCGCGGCTGATGCAGCGCTG
TGGGATGGAGTGGCTGTTTCGGCTGGCGCTGGAACCGCGCCGGCTGGCCTGGCGCTATCTGCGCACCAATGCGGCGTATC
TGCTGCTGTTGCTGCGCGCCCGCCTCAGCCGACGCTACCGCGCGCGGGCGCTGACGACCTGGCCCACATGA

Upstream 100 bases:

>100_bases
ATCTTGCAGCGAGCAAGCGCGCGCGCAAGTGGTGCTGGGATCGCTTGATCGAGAAGCCGCTGCTGCAAAGCGCACAACCC
ACCGTGCTGCTGTCGGCGTA

Downstream 100 bases:

>100_bases
CCGCTGGCGCGCCTGCGCGCCAGCCTGCGCAGACCGGGCTGGTGGCCGTGTGCCTGGGCCTCTACCTGCTGTTCTGTCTG
CAGCAAGTGGTGTTGACCGG

Product: UDP-N-acetyl-D-mannosamine transferase

Products: C55-PP-GlcNAc-ManNAcA; UDP [C]

Alternate protein names: UDP-ManNAcA transferase [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAAIPLTASAAAQARPAAAQCRARRRAVPARDVTATVARGSGAAVGRPPGRGQAATGCDRSPYPAEHGRSRVARGRTWPG
SGTPGSLRRGIGGRCTRALPWLRQRCRGCSDGGTGRCLRAAIGRRGHADRLDRNAAAWVSGGCQRHSRQSLDPGPDRPAG
GLAPARRQRRVGGMHSRATTAANWAGTHRACPGRFHLGGPASAGAGTVRCSGRKGTRNLTTSPARLSFLGCPLDLLPPEQ
ILLRAQHAAEGGAPIRVEGLNVAKLIDARAQLRQALEQAEVVHIDGAGISLGVRWFGVDVPPRRAGIDLMQDMCGQAARS
GAGVYLLGARPAVVAAAAERLCTHWPGLHIAGMRDGYFSDAEEDAVIAAIRQSGARYLFIGISSPKKELLLEKHWQTLGV
PVAMGVGGSFDVLSGMLPRAPRLMQRCGMEWLFRLALEPRRLAWRYLRTNAAYLLLLLRARLSRRYRARALTTWPT

Sequences:

>Translated_476_residues
MAAIPLTASAAAQARPAAAQCRARRRAVPARDVTATVARGSGAAVGRPPGRGQAATGCDRSPYPAEHGRSRVARGRTWPG
SGTPGSLRRGIGGRCTRALPWLRQRCRGCSDGGTGRCLRAAIGRRGHADRLDRNAAAWVSGGCQRHSRQSLDPGPDRPAG
GLAPARRQRRVGGMHSRATTAANWAGTHRACPGRFHLGGPASAGAGTVRCSGRKGTRNLTTSPARLSFLGCPLDLLPPEQ
ILLRAQHAAEGGAPIRVEGLNVAKLIDARAQLRQALEQAEVVHIDGAGISLGVRWFGVDVPPRRAGIDLMQDMCGQAARS
GAGVYLLGARPAVVAAAAERLCTHWPGLHIAGMRDGYFSDAEEDAVIAAIRQSGARYLFIGISSPKKELLLEKHWQTLGV
PVAMGVGGSFDVLSGMLPRAPRLMQRCGMEWLFRLALEPRRLAWRYLRTNAAYLLLLLRARLSRRYRARALTTWPT
>Mature_475_residues
AAIPLTASAAAQARPAAAQCRARRRAVPARDVTATVARGSGAAVGRPPGRGQAATGCDRSPYPAEHGRSRVARGRTWPGS
GTPGSLRRGIGGRCTRALPWLRQRCRGCSDGGTGRCLRAAIGRRGHADRLDRNAAAWVSGGCQRHSRQSLDPGPDRPAGG
LAPARRQRRVGGMHSRATTAANWAGTHRACPGRFHLGGPASAGAGTVRCSGRKGTRNLTTSPARLSFLGCPLDLLPPEQI
LLRAQHAAEGGAPIRVEGLNVAKLIDARAQLRQALEQAEVVHIDGAGISLGVRWFGVDVPPRRAGIDLMQDMCGQAARSG
AGVYLLGARPAVVAAAAERLCTHWPGLHIAGMRDGYFSDAEEDAVIAAIRQSGARYLFIGISSPKKELLLEKHWQTLGVP
VAMGVGGSFDVLSGMLPRAPRLMQRCGMEWLFRLALEPRRLAWRYLRTNAAYLLLLLRARLSRRYRARALTTWPT

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis [H]

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family [H]

Homologues:

Organism=Escherichia coli, GI2367289, Length=186, Percent_Identity=32.7956989247312, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023085
- InterPro:   IPR004629 [H]

Pfam domain/function: PF03808 Glyco_tran_WecB [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 50832; Mature: 50701

Theoretical pI: Translated: 12.03; Mature: 12.03

Prosite motif: PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIPLTASAAAQARPAAAQCRARRRAVPARDVTATVARGSGAAVGRPPGRGQAATGCDR
CCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
SPYPAEHGRSRVARGRTWPGSGTPGSLRRGIGGRCTRALPWLRQRCRGCSDGGTGRCLRA
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
AIGRRGHADRLDRNAAAWVSGGCQRHSRQSLDPGPDRPAGGLAPARRQRRVGGMHSRATT
HHCCCCCHHHHCCCHHHHHHCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
AANWAGTHRACPGRFHLGGPASAGAGTVRCSGRKGTRNLTTSPARLSFLGCPLDLLPPEQ
HHCCCCCCCCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHH
ILLRAQHAAEGGAPIRVEGLNVAKLIDARAQLRQALEQAEVVHIDGAGISLGVRWFGVDV
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEECCC
PPRRAGIDLMQDMCGQAARSGAGVYLLGARPAVVAAAAERLCTHWPGLHIAGMRDGYFSD
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
AEEDAVIAAIRQSGARYLFIGISSPKKELLLEKHWQTLGVPVAMGVGGSFDVLSGMLPRA
CCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCC
PRLMQRCGMEWLFRLALEPRRLAWRYLRTNAAYLLLLLRARLSRRYRARALTTWPT
HHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AAIPLTASAAAQARPAAAQCRARRRAVPARDVTATVARGSGAAVGRPPGRGQAATGCDR
CCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
SPYPAEHGRSRVARGRTWPGSGTPGSLRRGIGGRCTRALPWLRQRCRGCSDGGTGRCLRA
CCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
AIGRRGHADRLDRNAAAWVSGGCQRHSRQSLDPGPDRPAGGLAPARRQRRVGGMHSRATT
HHCCCCCHHHHCCCHHHHHHCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
AANWAGTHRACPGRFHLGGPASAGAGTVRCSGRKGTRNLTTSPARLSFLGCPLDLLPPEQ
HHCCCCCCCCCCCCEECCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHH
ILLRAQHAAEGGAPIRVEGLNVAKLIDARAQLRQALEQAEVVHIDGAGISLGVRWFGVDV
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEECCC
PPRRAGIDLMQDMCGQAARSGAGVYLLGARPAVVAAAAERLCTHWPGLHIAGMRDGYFSD
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
AEEDAVIAAIRQSGARYLFIGISSPKKELLLEKHWQTLGVPVAMGVGGSFDVLSGMLPRA
CCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHCCCC
PRLMQRCGMEWLFRLALEPRRLAWRYLRTNAAYLLLLLRARLSRRYRARALTTWPT
HHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosaminuronic-acid; undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate [C]

Specific reaction: UDP-N-acetyl-D-mannosaminuronic-acid + undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate = C55-PP-GlcNAc-ManNAcA + UDP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA