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The map label for this gene is tetA [H]

Identifier: 21245007

GI number: 21245007

Start: 5075447

End: 5076712

Strand: Reverse

Name: tetA [H]

Synonym: XAC4295

Alternate gene names: 21245007

Gene position: 5076712-5075447 (Counterclockwise)

Preceding gene: 77748773

Following gene: 21245006

Centisome position: 98.09

GC content: 68.01

Gene sequence:

>1266_bases
ATGTCCCTCTCTCCCGATTCCGCTCCCGTCCCGGGGCGCCGCCGCGCCGCGCTGATCTTCATCTTCATCACCGTCCTGAT
CGACGTGCTGTCCTTCGGGGTCATCATTCCGGTACTCCCGGATCTGGTGCGCCACTTCACCGGTGGCGACTACGTCGTGG
CCGCCGGCTGGATCGGCTGGTTCGGCTTCCTGTTCGCTGCCATCCAGTTCGTCTGCTCGCCGTTGCAGGGCGCGCTCTCC
GACCGCTTCGGTCGCCGCCCGGTGATCCTGCTCTCGTGCCTTGGCCTGGGCCTGGACTTCATCCTGATGGCCATTGCCCA
CAGCCTGCCGATGCTGCTGCTGGCGCGGGTCATCTCCGGCGTGTGCTCGGCCAGCTTCTCCACCGCCAATGCCTACATCG
CCGATGTCACCCCACCGGACAAACGCGCCGGCGCATTCGGCATGCTTGGTGCCGCCTTCGGCATCGGCTTTGTCGCCGGC
CCGTTGATCGGCGGTTGGCTGGGCAGCATCGGGTTGCGCTGGCCGTTCTGGTTTGCCGCCGGGCTTGCCCTGTTGAACGT
GCTGTATGGCTGGTTCGTGCTGCCCGAATCGCTGCCCGCCCAACGCCGCACCGCGCGGCTGGACTGGTCGCACGCCAATC
CGCTGGGCGCGCTCAAACTGCTGCGCCGCTACCCGCAGGTGTTCGGCCTGGCGTCGGTGGTGTTCCTGGCCAACCTGGCG
CATTACGTCTATCCCAGCATTTTCGTGCTGTTCGCCGGTTACCAATACCATTGGGGCCCGCGCGAAGTGAGCTGGGTACT
GGCCGGGGTCGGTATCTGCAGCATCATCGTCAATGCGCTGCTGGTCGGCCGCCTGGTGCGCCGGCTCGGCGAACGCCGCG
CGTTGCTGCTGGGGCTGGGCTGCGGGGTGATCGGCTTCATCATCTATGGGCTGGCCGACAGCGGCACTGCCTTCCTGATC
GGTGTGCCGATCAGTGCGTTGTGGGCCATTGCCGCACCGTCCGCGCAGGCCCTGATCACCCGTGAGGTCGGCGCCGACGC
GCAAGGTCGCGTGCAGGGCGCGCTCACCGGTCTGGTCAGCCTGGCCGGCATCGTCGGTCCACTGTTGTTCGCCAATGTCT
TCGCCTGGTTCATCGGCAGCGGTGCGCCGCTGCATCTGCCGGGCGCGCCGTGGTTGCTGGCTGCTGTCCTGCTGGCCGCA
GGCTGGGGCATGGCCTGGAAACGGGCCGCGCGCGGCGCCGGCACCACACCCGCGGCGCAGACGTGA

Upstream 100 bases:

>100_bases
GCAGCCCGAACGACAGCAGGTTTCCCAGCAACCACGTGCCACCAGCATCGACCACCCAATCCCTCGCTACACTCGCCGCT
TCGCCCAACTGCGCCGCGCG

Downstream 100 bases:

>100_bases
AGCCAACCTATTCAGCTTGCGCTCGCGTCTGCTTTTTCTGATTTGCGCAGGCACTGCTAGTCTGGTGTCCTTCGTGCCGC
GGCACCGCCTTGAGCCCCTA

Product: tetracycline-efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(A) [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSLSPDSAPVPGRRRAALIFIFITVLIDVLSFGVIIPVLPDLVRHFTGGDYVVAAGWIGWFGFLFAAIQFVCSPLQGALS
DRFGRRPVILLSCLGLGLDFILMAIAHSLPMLLLARVISGVCSASFSTANAYIADVTPPDKRAGAFGMLGAAFGIGFVAG
PLIGGWLGSIGLRWPFWFAAGLALLNVLYGWFVLPESLPAQRRTARLDWSHANPLGALKLLRRYPQVFGLASVVFLANLA
HYVYPSIFVLFAGYQYHWGPREVSWVLAGVGICSIIVNALLVGRLVRRLGERRALLLGLGCGVIGFIIYGLADSGTAFLI
GVPISALWAIAAPSAQALITREVGADAQGRVQGALTGLVSLAGIVGPLLFANVFAWFIGSGAPLHLPGAPWLLAAVLLAA
GWGMAWKRAARGAGTTPAAQT

Sequences:

>Translated_421_residues
MSLSPDSAPVPGRRRAALIFIFITVLIDVLSFGVIIPVLPDLVRHFTGGDYVVAAGWIGWFGFLFAAIQFVCSPLQGALS
DRFGRRPVILLSCLGLGLDFILMAIAHSLPMLLLARVISGVCSASFSTANAYIADVTPPDKRAGAFGMLGAAFGIGFVAG
PLIGGWLGSIGLRWPFWFAAGLALLNVLYGWFVLPESLPAQRRTARLDWSHANPLGALKLLRRYPQVFGLASVVFLANLA
HYVYPSIFVLFAGYQYHWGPREVSWVLAGVGICSIIVNALLVGRLVRRLGERRALLLGLGCGVIGFIIYGLADSGTAFLI
GVPISALWAIAAPSAQALITREVGADAQGRVQGALTGLVSLAGIVGPLLFANVFAWFIGSGAPLHLPGAPWLLAAVLLAA
GWGMAWKRAARGAGTTPAAQT
>Mature_420_residues
SLSPDSAPVPGRRRAALIFIFITVLIDVLSFGVIIPVLPDLVRHFTGGDYVVAAGWIGWFGFLFAAIQFVCSPLQGALSD
RFGRRPVILLSCLGLGLDFILMAIAHSLPMLLLARVISGVCSASFSTANAYIADVTPPDKRAGAFGMLGAAFGIGFVAGP
LIGGWLGSIGLRWPFWFAAGLALLNVLYGWFVLPESLPAQRRTARLDWSHANPLGALKLLRRYPQVFGLASVVFLANLAH
YVYPSIFVLFAGYQYHWGPREVSWVLAGVGICSIIVNALLVGRLVRRLGERRALLLGLGCGVIGFIIYGLADSGTAFLIG
VPISALWAIAAPSAQALITREVGADAQGRVQGALTGLVSLAGIVGPLLFANVFAWFIGSGAPLHLPGAPWLLAAVLLAAG
WGMAWKRAARGAGTTPAAQT

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=316, Percent_Identity=32.2784810126582, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI94721311, Length=408, Percent_Identity=29.4117647058824, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI225703104, Length=167, Percent_Identity=35.3293413173653, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI225703102, Length=167, Percent_Identity=35.3293413173653, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1787291, Length=388, Percent_Identity=25, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71996331, Length=378, Percent_Identity=28.042328042328, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17551570, Length=228, Percent_Identity=23.6842105263158, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357569, Length=365, Percent_Identity=29.3150684931507, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24656463, Length=365, Percent_Identity=29.3150684931507, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24656468, Length=365, Percent_Identity=29.3150684931507, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161080926, Length=365, Percent_Identity=29.3150684931507, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161080924, Length=365, Percent_Identity=29.3150684931507, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI221513297, Length=360, Percent_Identity=28.0555555555556, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17738085, Length=419, Percent_Identity=26.0143198090692, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24667557, Length=163, Percent_Identity=28.8343558282209, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24667561, Length=166, Percent_Identity=27.710843373494, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44453; Mature: 44322

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS00141 ASP_PROTEASE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure 
SLSPDSAPVPGRRRAALIFIFITVLIDVLSFGVIIPVLPDLVRHFTGGDYVVAAGWIGW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHH
FGFLFAAIQFVCSPLQGALSDRFGRRPVILLSCLGLGLDFILMAIAHSLPMLLLARVISG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCSASFSTANAYIADVTPPDKRAGAFGMLGAAFGIGFVAGPLIGGWLGSIGLRWPFWFAA
HHHCCCCCCCCEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
GLALLNVLYGWFVLPESLPAQRRTARLDWSHANPLGALKLLRRYPQVFGLASVVFLANLA
HHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYVYPSIFVLFAGYQYHWGPREVSWVLAGVGICSIIVNALLVGRLVRRLGERRALLLGLG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGVIGFIIYGLADSGTAFLIGVPISALWAIAAPSAQALITREVGADAQGRVQGALTGLVS
HHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
LAGIVGPLLFANVFAWFIGSGAPLHLPGAPWLLAAVLLAAGWGMAWKRAARGAGTTPAAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312499; 6310527 [H]