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The map label for this gene is aceK

Identifier: 21244556

GI number: 21244556

Start: 4507087

End: 4508826

Strand: Reverse

Name: aceK

Synonym: XAC3832

Alternate gene names: 21244556

Gene position: 4508826-4507087 (Counterclockwise)

Preceding gene: 21244557

Following gene: 21244553

Centisome position: 87.12

GC content: 62.76

Gene sequence:

>1740_bases
ATGAACCATCCATCGTTGCCACCACAATCCGAGCGCCGTGCGCTGGCGATCGCCCAGGCCGTGTACGAAGCGTTCGAGGA
CTATCACGCGCGCTTTTCCGAAATCACCGCATGCGCCAAACAGCGGTTCGAAACACGCGATTGGAGCGGCGCGCGCGAAG
ACGCAGTGGCGCGCATCGCGCTCTACGACCAATACATCGCCGAATGCATGTTGCGTCTGCGCGCAGTACTGCTGGGTCAG
GCGCACGACCGCGCGCTATGGATGCGTGCGCGCGATCGTTACGCTGCGTTGCTGAGCGGGTTGATCGACCAGGAGTTGTA
CAAGACGTTCTACAACACCCTGACCCGGCGCTACTTCGGTACCCACGGCGTCGATGCGGACATCGAATTCATCGCCCTGG
ATATCGAACCGACCGACGCCATCACCGTGCCTGTTGCGCGCCACACCTATGCGGTCTCGCCCGGCCGCCTGACCGACATG
CTGGTGCGGGTGCTGGGCGACTACGCGTTCGCCGTGCCGTATGCGCACCGCACCCGCTGCGCGGCTGCCATCGCGGTGCG
TCTGCTAGACGACCTGGCGCATTGGGGCGAGCATCCGGTGCGCAGCGTGGAATTGCTGGAGACGGTGTTCTATCGCGAGC
GGCGTGCGTATCTGGTCGGACGCGTGTTCGGCGAACATCGCTTTTCGCCATGCGTGATCGCGCTGATCAACGACGACGCC
GGCCTGCGCGCCGAAGCGGTGCTGACCCGACGCAGCGATGTCGCGCAATTGTTCAGTAACTCGCGCAGCTATTTTCAAGC
CGACCTGTCCACTGTCGGCGATGCGGTGGTGTTCCTGCGCAGCCTGCTCACCCACAAACCGGTCGATGAGCTATACACGA
TGCTGGGCCGCGCCAAGCAGGGCAAGACCGAGCGTTACCGCACGTTTTTCAGCCATTTTCAGGCGCATCCGTCCGAACAG
CTGGTGCACGCCGATGGCACTCCCGGCATGGTCATGGTGGTCTTTACGCTGCCCAGTTATCCGCTGGTCTTCAAACTCAT
TCGCGACCGCTTTGCGTATCCCAAAACCATGAGCCGCGCGCAGGTGGAGGGTAAGTACGAATTGGTCTTCCAGCTCGACC
GCATCGGCCGCCTGCTCGACGCGCAGCCGTATCGTTTCCTGCGTTTCCCCAAGGCGCGCTTTTCGCCGGCGCTGCTGCAA
GAGCTGCAGACCAGCTGCGCGATGAGCCTGAGCGAAGACGGCGACGATGTCCTGATCGCACTGTGCTATGTGCAGCGCCG
GTTGCGCCCGTTGAATTTGTACCTGCGCGAACAGTTGCCCGAAGCCGCGCATGCCGCAGCGCTGGACTACGGCCAAGCCA
TCAAGGACATGGCGCGCAACAACATCTTTCCCGGCGACATGTTGCTGAAGAATTTCGGCATTACCCGCCATCAGCGCGCG
GTGTTCTACGACTACGACGAGCTGTGCCTGATCACCGAATGCAACTTCCGCGATTGGCCGACACCCGCCACCTACGAAGA
ACAGATGGCCGCAGAACCCTGGTTCCATGTCGGCCCGCGCGACGTCTTTCCCGAGCGCTTCGCCTTGTTCATGGGCCTGC
CCTCCTCGCAACTGGAAGCGGTCAAGCACCAGCACCCGGAACTGTTCGACCCACGCTGGTGGCGCGACCTGCAGAGCCGG
CTTCGCGACGACGATTATCCCGACACCCCGCCATATGCGGAGTCGCGCAGGCTGGCCTAG

Upstream 100 bases:

>100_bases
GGCTACAGGGCCAGGCGCGCGAACACGCCGCGCATGCGCAACAGGCGACCGCCACGCCATTGGCCGCGCGCCGCCGCAAG
CGCTCCGGGACACCGTCGCC

Downstream 100 bases:

>100_bases
AGCGCTGCGGAGCGCTCGCCGCGCAGCTTACCTTGGCGCCAGCCCAGCCCAGCCCAGCCGAGCCGGCGCCACGCAGCACC
CGCTCCGCACAGCTGGCGGC

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MNHPSLPPQSERRALAIAQAVYEAFEDYHARFSEITACAKQRFETRDWSGAREDAVARIALYDQYIAECMLRLRAVLLGQ
AHDRALWMRARDRYAALLSGLIDQELYKTFYNTLTRRYFGTHGVDADIEFIALDIEPTDAITVPVARHTYAVSPGRLTDM
LVRVLGDYAFAVPYAHRTRCAAAIAVRLLDDLAHWGEHPVRSVELLETVFYRERRAYLVGRVFGEHRFSPCVIALINDDA
GLRAEAVLTRRSDVAQLFSNSRSYFQADLSTVGDAVVFLRSLLTHKPVDELYTMLGRAKQGKTERYRTFFSHFQAHPSEQ
LVHADGTPGMVMVVFTLPSYPLVFKLIRDRFAYPKTMSRAQVEGKYELVFQLDRIGRLLDAQPYRFLRFPKARFSPALLQ
ELQTSCAMSLSEDGDDVLIALCYVQRRLRPLNLYLREQLPEAAHAAALDYGQAIKDMARNNIFPGDMLLKNFGITRHQRA
VFYDYDELCLITECNFRDWPTPATYEEQMAAEPWFHVGPRDVFPERFALFMGLPSSQLEAVKHQHPELFDPRWWRDLQSR
LRDDDYPDTPPYAESRRLA

Sequences:

>Translated_579_residues
MNHPSLPPQSERRALAIAQAVYEAFEDYHARFSEITACAKQRFETRDWSGAREDAVARIALYDQYIAECMLRLRAVLLGQ
AHDRALWMRARDRYAALLSGLIDQELYKTFYNTLTRRYFGTHGVDADIEFIALDIEPTDAITVPVARHTYAVSPGRLTDM
LVRVLGDYAFAVPYAHRTRCAAAIAVRLLDDLAHWGEHPVRSVELLETVFYRERRAYLVGRVFGEHRFSPCVIALINDDA
GLRAEAVLTRRSDVAQLFSNSRSYFQADLSTVGDAVVFLRSLLTHKPVDELYTMLGRAKQGKTERYRTFFSHFQAHPSEQ
LVHADGTPGMVMVVFTLPSYPLVFKLIRDRFAYPKTMSRAQVEGKYELVFQLDRIGRLLDAQPYRFLRFPKARFSPALLQ
ELQTSCAMSLSEDGDDVLIALCYVQRRLRPLNLYLREQLPEAAHAAALDYGQAIKDMARNNIFPGDMLLKNFGITRHQRA
VFYDYDELCLITECNFRDWPTPATYEEQMAAEPWFHVGPRDVFPERFALFMGLPSSQLEAVKHQHPELFDPRWWRDLQSR
LRDDDYPDTPPYAESRRLA
>Mature_579_residues
MNHPSLPPQSERRALAIAQAVYEAFEDYHARFSEITACAKQRFETRDWSGAREDAVARIALYDQYIAECMLRLRAVLLGQ
AHDRALWMRARDRYAALLSGLIDQELYKTFYNTLTRRYFGTHGVDADIEFIALDIEPTDAITVPVARHTYAVSPGRLTDM
LVRVLGDYAFAVPYAHRTRCAAAIAVRLLDDLAHWGEHPVRSVELLETVFYRERRAYLVGRVFGEHRFSPCVIALINDDA
GLRAEAVLTRRSDVAQLFSNSRSYFQADLSTVGDAVVFLRSLLTHKPVDELYTMLGRAKQGKTERYRTFFSHFQAHPSEQ
LVHADGTPGMVMVVFTLPSYPLVFKLIRDRFAYPKTMSRAQVEGKYELVFQLDRIGRLLDAQPYRFLRFPKARFSPALLQ
ELQTSCAMSLSEDGDDVLIALCYVQRRLRPLNLYLREQLPEAAHAAALDYGQAIKDMARNNIFPGDMLLKNFGITRHQRA
VFYDYDELCLITECNFRDWPTPATYEEQMAAEPWFHVGPRDVFPERFALFMGLPSSQLEAVKHQHPELFDPRWWRDLQSR
LRDDDYPDTPPYAESRRLA

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=566, Percent_Identity=34.6289752650177, Blast_Score=336, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_XANAC (Q8PFZ0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644138.1
- GeneID:   1157903
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3832
- NMPDR:   fig|190486.1.peg.3782
- HOGENOM:   HBG298593
- OMA:   RMTPLNI
- ProtClustDB:   PRK02946
- BioCyc:   XAXO190486:XAC3832-MONOMER
- BRENDA:   2.7.11.5
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 66729; Mature: 66729

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: ACT_SITE 380-380 BINDING 345-345

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPSLPPQSERRALAIAQAVYEAFEDYHARFSEITACAKQRFETRDWSGAREDAVARIA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LYDQYIAECMLRLRAVLLGQAHDRALWMRARDRYAALLSGLIDQELYKTFYNTLTRRYFG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THGVDADIEFIALDIEPTDAITVPVARHTYAVSPGRLTDMLVRVLGDYAFAVPYAHRTRC
CCCCCCCEEEEEEEECCCCCEEECEECCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AAAIAVRLLDDLAHWGEHPVRSVELLETVFYRERRAYLVGRVFGEHRFSPCVIALINDDA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GLRAEAVLTRRSDVAQLFSNSRSYFQADLSTVGDAVVFLRSLLTHKPVDELYTMLGRAKQ
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
GKTERYRTFFSHFQAHPSEQLVHADGTPGMVMVVFTLPSYPLVFKLIRDRFAYPKTMSRA
CCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHH
QVEGKYELVFQLDRIGRLLDAQPYRFLRFPKARFSPALLQELQTSCAMSLSEDGDDVLIA
HCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCHHHHH
LCYVQRRLRPLNLYLREQLPEAAHAAALDYGQAIKDMARNNIFPGDMLLKNFGITRHQRA
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCE
VFYDYDELCLITECNFRDWPTPATYEEQMAAEPWFHVGPRDVFPERFALFMGLPSSQLEA
EEECCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCHHHCHHHHHHHHCCCHHHHHH
VKHQHPELFDPRWWRDLQSRLRDDDYPDTPPYAESRRLA
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNHPSLPPQSERRALAIAQAVYEAFEDYHARFSEITACAKQRFETRDWSGAREDAVARIA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LYDQYIAECMLRLRAVLLGQAHDRALWMRARDRYAALLSGLIDQELYKTFYNTLTRRYFG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
THGVDADIEFIALDIEPTDAITVPVARHTYAVSPGRLTDMLVRVLGDYAFAVPYAHRTRC
CCCCCCCEEEEEEEECCCCCEEECEECCEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AAAIAVRLLDDLAHWGEHPVRSVELLETVFYRERRAYLVGRVFGEHRFSPCVIALINDDA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GLRAEAVLTRRSDVAQLFSNSRSYFQADLSTVGDAVVFLRSLLTHKPVDELYTMLGRAKQ
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
GKTERYRTFFSHFQAHPSEQLVHADGTPGMVMVVFTLPSYPLVFKLIRDRFAYPKTMSRA
CCHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHH
QVEGKYELVFQLDRIGRLLDAQPYRFLRFPKARFSPALLQELQTSCAMSLSEDGDDVLIA
HCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCHHHHHHHHHHHHHHCCCCCCHHHHH
LCYVQRRLRPLNLYLREQLPEAAHAAALDYGQAIKDMARNNIFPGDMLLKNFGITRHQRA
HHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCE
VFYDYDELCLITECNFRDWPTPATYEEQMAAEPWFHVGPRDVFPERFALFMGLPSSQLEA
EEECCCCEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCHHHCHHHHHHHHCCCHHHHHH
VKHQHPELFDPRWWRDLQSRLRDDDYPDTPPYAESRRLA
HHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217