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The map label for this gene is ampR [H]

Identifier: 21244557

GI number: 21244557

Start: 4508823

End: 4509770

Strand: Reverse

Name: ampR [H]

Synonym: XAC3833

Alternate gene names: 21244557

Gene position: 4509770-4508823 (Counterclockwise)

Preceding gene: 21244558

Following gene: 21244556

Centisome position: 87.14

GC content: 67.83

Gene sequence:

>948_bases
ATGTCGCGTCCGCCGCTTCACGCATTGCAAGGCTTCGTCAGCGCGGTACGCCTGGGTAACCTGTCGCGCGCCGCTGGCGC
GATGCATCTGACCGTCAGCGCACTTAGTCATCAGATGCGCGCGCTCGAACAACGGCTGGGATATCCGCTACTGCAACGGC
ATGCGCGCGGCGTCACCGCCACGCCGCAGGGCCAGCAACTGCTCGACCGCATCGCACCGCATCTGGACGCCATCGCCGAG
GCGTTCGAGCCCTTCGGTGCGCGTCGCGAAGACACCCTGACGCTGAGTCTCACGCCGTCGATGGCATCGGCCTGGCTGGT
GCCGCGCCTGGGCGAATTTCTGGCAAGTCATCCCACCATCGAAATCAACCTGCTGTCGAGCGAGCGGCTGGTGGATTTCG
AGCGCCAGCAGCAAGTGGATGCCGCCATGCGTATCGGCGCCGGGCAATGGCCTGGCGTCATCGCCGAGCCATTGTTCGAC
GAATGGCTGGTGCCCATGGCTAGCCCGGCCCTGATCGCCCGCATGGGCGGCGTGGAGGCGCCACCCTTGCATCAGTGGCC
ACTGCTGGGCGACCCGGATGACGCCTGGTCGCGCTGGTTCGCGGCGTTCGGCGGCGATGTGCCGCGACGCTATGTCGCCG
TGCTCGACGAATCGGAGGCACACCATCGTGCCGCCTTGGATGGCGTCGGCGTCGCACTGGGGCGCGTGACGCGCGCGCGT
CTGTTGCTGGCGTCCGGCCAGCTGGTGGCGTTGTCCACGCAACGGCTCAAGACCAGTTGGTCGCATTACCTGGTGTATCC
GCCGCGTTCCAGCACCCATGGCGGGTTTCTCGCGTTTCGCACCTGGCTACAGGGCCAGGCGCGCGAACACGCCGCGCATG
CGCAACAGGCGACCGCCACGCCATTGGCCGCGCGCCGCCGCAAGCGCTCCGGGACACCGTCGCCATGA

Upstream 100 bases:

>100_bases
ACCCACCTCCACTTGAGTGGAATCCACAGTAGGCGCAGGCTAGGGGACGTAAAAGCGAGGCTTTTACAAGTGAGTATTGA
ATTCCATTCAAGGGTTGGTC

Downstream 100 bases:

>100_bases
ACCATCCATCGTTGCCACCACAATCCGAGCGCCGTGCGCTGGCGATCGCCCAGGCCGTGTACGAAGCGTTCGAGGACTAT
CACGCGCGCTTTTCCGAAAT

Product: B-lactamase regulatory protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRALEQRLGYPLLQRHARGVTATPQGQQLLDRIAPHLDAIAE
AFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLASHPTIEINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFD
EWLVPMASPALIARMGGVEAPPLHQWPLLGDPDDAWSRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATATPLAARRRKRSGTPSP

Sequences:

>Translated_315_residues
MSRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRALEQRLGYPLLQRHARGVTATPQGQQLLDRIAPHLDAIAE
AFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLASHPTIEINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFD
EWLVPMASPALIARMGGVEAPPLHQWPLLGDPDDAWSRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATATPLAARRRKRSGTPSP
>Mature_314_residues
SRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRALEQRLGYPLLQRHARGVTATPQGQQLLDRIAPHLDAIAEA
FEPFGARREDTLTLSLTPSMASAWLVPRLGEFLASHPTIEINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFDE
WLVPMASPALIARMGGVEAPPLHQWPLLGDPDDAWSRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRARL
LLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATATPLAARRRKRSGTPSP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=32.6388888888889, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=32.0689655172414, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=30.794701986755, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=28.3276450511945, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786401, Length=252, Percent_Identity=28.1746031746032, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=26.3333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=29.375, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=149, Percent_Identity=31.5436241610738, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34449; Mature: 34318

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRALEQRLGYPLLQRHARGVTA
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
TPQGQQLLDRIAPHLDAIAEAFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLASHPTI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCE
EINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFDEWLVPMASPALIARMGGVEA
EEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PPLHQWPLLGDPDDAWSRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATAT
HHHCCCCEEEEEHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PLAARRRKRSGTPSP
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRPPLHALQGFVSAVRLGNLSRAAGAMHLTVSALSHQMRALEQRLGYPLLQRHARGVTA
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
TPQGQQLLDRIAPHLDAIAEAFEPFGARREDTLTLSLTPSMASAWLVPRLGEFLASHPTI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCE
EINLLSSERLVDFERQQQVDAAMRIGAGQWPGVIAEPLFDEWLVPMASPALIARMGGVEA
EEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PPLHQWPLLGDPDDAWSRWFAAFGGDVPRRYVAVLDESEAHHRAALDGVGVALGRVTRAR
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LLLASGQLVALSTQRLKTSWSHYLVYPPRSSTHGGFLAFRTWLQGQAREHAAHAQQATAT
HHHCCCCEEEEEHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PLAARRRKRSGTPSP
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]