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The map label for this gene is lpdA [H]

Identifier: 21244384

GI number: 21244384

Start: 4336277

End: 4338100

Strand: Reverse

Name: lpdA [H]

Synonym: XAC3659

Alternate gene names: 21244384

Gene position: 4338100-4336277 (Counterclockwise)

Preceding gene: 21244385

Following gene: 21244383

Centisome position: 83.82

GC content: 67.11

Gene sequence:

>1824_bases
ATGGCGGTCATTGAAATCAAGGTTCCCGACATCGGCGATTACAGCGATGTCCCCGTCATCGAAGTGCTGGTCGCCGTTGG
CGACAGCGTGGCCAAGGACCAGGGACTGGTAACGCTGGAATCGGACAAGGCGACGCTGGAAGTGCCGTCCTCGGCCGCCG
GGGTGGTCAAGGAACTCAAGGTCAAGGTCGGCGACACCCTGTCCGAAGGCGCGCTCGTGCTGCTGCTCGAAACCGAAGGC
GAGGCTGCCGCGCCGGCCAAGGCCGAACCCAAGGCCGCCCCGGCCGCTGCAGCGCCTGCCGCCGCACCGGGCAGCAAGCC
GCCGGTGACGCCGTCGCACCGCGCCCCGGCAGAGCCGGCCGCGCCCAAACCGGCGCTGGCCAGCGGCAAGCCGGCCGACA
TCGAATGCAAGATGGTGGTGCTCGGCGCCGGCCCCGGCGGCTACACCGCTGCGTTCCGCGCGGCCGACCTGGGCCTGGAC
ACTGTGCTGATCGAGCGCTACGCCAGCCTGGGCGGTGTCTGCCTCAACGTCGGCTGCATCCCGTCCAAGGCGCTGCTGCA
TGCCGCCGCGGTGATCGATGAAGTGGCCCATGCCGGCGACTTCGGCGTGGACTTCGGTCAGCCCAAGATCACCCTGGACA
AGCTGCGCGAGTACAAGGAAAAAGTGGTCGGCAAGCTCACCGGCGGCCTGGCCAGCATGGCCAAGCAGCGCAAGGTGCGC
ACCGTGACCGGCGTGGCCAGCTTCGTCTCGCCCAACGAGCTGGAAATCGTCGGCGACGACGGCAAGACCCAGCTGCTGCG
CTTCGAGCACTGCATCATCGCCGCCGGCTCGCAGGCAGTGAAGCTGCCCAACTTCCCGTGGGACGACAAGCGCGTGATGG
ACTCCACCGACGCGCTGGAGCTGCACGACATTCCCAAGACCCTCCTGGTGGTCGGCGGCGGCATCATCGGCCTGGAAATG
GCCACGGTGTACAGCGCGCTCGGCAGCAAGGTGACCGTGGTCGAGTTCATGGACCAGCTGATGCCGGGCGCCGACAAGGA
CCTGGTCAAGCCGCTGGCCGACCGCCTGAAAAAGCAGGGCGTCGAGGTCCATCTCAAGACCAAGGCCACCGACGTCAAGG
CCGACAAGAGCGGCATCACGGTGTCCTTCGAGGCGGCTGTGGAAGGCGAAAAGCCCGGTCTGCAGGCCACCGCGTACGAC
CGCGTGCTGGTCGCCGTGGGCCGTAGCCCGAACGGCAAGAAGATCGGTGCGGAAAAGGCCGGCGTCACCATCACCGAGCG
CGGCTTCATTCCGGTCGATCGGCAGATGCGCACCAACGTGCCGCACATCTTCGCCATCGGCGACATCGTCGGTAACCCGA
TGCTGGCGCACAAGGCCACCCACGAAGGCAAGCTGGCCGCCGAAGTGGCTGCCGGCGAGAAAAAGGAATGGGTGGCGCGG
GTGATCCCGTCGGTGGCTTACACCAACCCGGAAATCGCCTGGGTCGGCGTCACCGAAACCGAAGCCAAGGCCAAGGGCCT
GAAGGTGGGCGTGGCCAAGTTCCCGTGGGCCGCCAGCGGCCGCGCGATCGGCATCGGCCGCACCGAGGGCTTCACCAAGC
TGATCTTCGATGAACAGACCCATCGCGTGATCGGCGGCGCCATCGTCGGCGTGCATGCCGGCGACCTGCTGGCCGAAATC
GGCCTGGCGATCGAGATGGGGGCCGAGGCCGAAGACATCGGCCACACCATCCACGCGCATCCGACCCTGAGCGAGTCGGT
CGGCATGGCCGCCGAGGTCTACGACGGCACCATCACCGATCTGTACATCCCCAAGAAGAAGTGA

Upstream 100 bases:

>100_bases
GAAAAGAAGCGCGACAAGCGCTGGACCCAAATCGAATCGTCGCTGGATGAACTCCAGCAGACGCTTGCCGGCACGGCCGG
TTGAGCTACAGGAGAAGCAA

Downstream 100 bases:

>100_bases
CGTCGCTCCGCACGACCGTGCCGCCACGCGTGGCGCGGTCGCCGCGGTGGTGACTCGATGCGTGGAGGTCGCCGTGGAGC
AGGGCAATCTGTGTCCCGAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MAVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELKVKVGDTLSEGALVLLLETEG
EAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPAAPKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLD
TVLIERYASLGGVCLNVGCIPSKALLHAAAVIDEVAHAGDFGVDFGQPKITLDKLREYKEKVVGKLTGGLASMAKQRKVR
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALELHDIPKTLLVVGGGIIGLEM
ATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQGVEVHLKTKATDVKADKSGITVSFEAAVEGEKPGLQATAYD
RVLVAVGRSPNGKKIGAEKAGVTITERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQTHRVIGGAIVGVHAGDLLAEI
GLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITDLYIPKKK

Sequences:

>Translated_607_residues
MAVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELKVKVGDTLSEGALVLLLETEG
EAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPAAPKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLD
TVLIERYASLGGVCLNVGCIPSKALLHAAAVIDEVAHAGDFGVDFGQPKITLDKLREYKEKVVGKLTGGLASMAKQRKVR
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALELHDIPKTLLVVGGGIIGLEM
ATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQGVEVHLKTKATDVKADKSGITVSFEAAVEGEKPGLQATAYD
RVLVAVGRSPNGKKIGAEKAGVTITERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQTHRVIGGAIVGVHAGDLLAEI
GLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITDLYIPKKK
>Mature_606_residues
AVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELKVKVGDTLSEGALVLLLETEGE
AAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPAAPKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLDT
VLIERYASLGGVCLNVGCIPSKALLHAAAVIDEVAHAGDFGVDFGQPKITLDKLREYKEKVVGKLTGGLASMAKQRKVRT
VTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALELHDIPKTLLVVGGGIIGLEMA
TVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQGVEVHLKTKATDVKADKSGITVSFEAAVEGEKPGLQATAYDR
VLVAVGRSPNGKKIGAEKAGVTITERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVARV
IPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQTHRVIGGAIVGVHAGDLLAEIG
LAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITDLYIPKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=41.703056768559, Blast_Score=346, Evalue=4e-95,
Organism=Homo sapiens, GI50301238, Length=450, Percent_Identity=28.4444444444444, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=28.4722222222222, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI22035672, Length=437, Percent_Identity=29.0617848970252, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI291045268, Length=422, Percent_Identity=27.0142180094787, Blast_Score=127, Evalue=3e-29,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=60.8421052631579, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=28.6610878661088, Blast_Score=186, Evalue=6e-48,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=28.5087719298246, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=27.9545454545455, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1786305, Length=78, Percent_Identity=56.4102564102564, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=41.505376344086, Blast_Score=350, Evalue=8e-97,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=29.4491525423729, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=27.3755656108597, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=27.3755656108597, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=442, Percent_Identity=25.5656108597285, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=41.5611814345992, Blast_Score=328, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=28.6012526096033, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=29.1938997821351, Blast_Score=169, Evalue=8e-43,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=43.1072210065646, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640551, Length=518, Percent_Identity=27.2200772200772, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640549, Length=431, Percent_Identity=27.8422273781903, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=27.1047227926078, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737741, Length=483, Percent_Identity=25.6728778467909, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 63214; Mature: 63083

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELK
CEEEEEECCCCCCCCCCHHHHHHHHHCCCHHCCCCCEEEECCCCEEECCCHHHHHHHHHH
VKVGDTLSEGALVLLLETEGEAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPA
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLDTVLIERYASLGGVCLNVGCI
CCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCC
PSKALLHAAAVIDEVAHAGDFGVDFGQPKITLDKLREYKEKVVGKLTGGLASMAKQRKVR
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALE
HHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCHHCCCCCCEE
LHDIPKTLLVVGGGIIGLEMATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQG
ECCCCCEEEHCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCC
VEVHLKTKATDVKADKSGITVSFEAAVEGEKPGLQATAYDRVLVAVGRSPNGKKIGAEKA
CEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEHHCEEEEEECCCCCCCCCCCCCC
GVTITERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
CEEEEECCCCCCCHHHHCCCCEEEEEHHHHCCCHHEECCCCCCCEEEHHHCCCHHHHHHH
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQT
HHHHHHCCCCCEEEEECCCCHHHHCCEEEEHEECCCCCCCCEEEECCCCCCEEEEECCCH
HRVIGGAIVGVHAGDLLAEIGLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITD
HHEECEEEEEEEHHHHHHHHCCEEECCCCHHHHCCEEECCCCCHHHHCCEEEECCCCEEE
LYIPKKK
EEECCCC
>Mature Secondary Structure 
AVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELK
EEEEEECCCCCCCCCCHHHHHHHHHCCCHHCCCCCEEEECCCCEEECCCHHHHHHHHHH
VKVGDTLSEGALVLLLETEGEAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPA
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLDTVLIERYASLGGVCLNVGCI
CCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCC
PSKALLHAAAVIDEVAHAGDFGVDFGQPKITLDKLREYKEKVVGKLTGGLASMAKQRKVR
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALE
HHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCHHCCCCCCEE
LHDIPKTLLVVGGGIIGLEMATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQG
ECCCCCEEEHCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCC
VEVHLKTKATDVKADKSGITVSFEAAVEGEKPGLQATAYDRVLVAVGRSPNGKKIGAEKA
CEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCEEEHHCEEEEEECCCCCCCCCCCCCC
GVTITERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
CEEEEECCCCCCCHHHHCCCCEEEEEHHHHCCCHHEECCCCCCCEEEHHHCCCHHHHHHH
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQT
HHHHHHCCCCCEEEEECCCCHHHHCCEEEEHEECCCCCCCCEEEECCCCCCEEEEECCCH
HRVIGGAIVGVHAGDLLAEIGLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITD
HHEECEEEEEEEHHHHHHHHCCEEECCCCHHHHCCEEECCCCCHHHHCCEEEECCCCEEE
LYIPKKK
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]