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The map label for this gene is bioD

Identifier: 21244341

GI number: 21244341

Start: 4290019

End: 4290693

Strand: Reverse

Name: bioD

Synonym: XAC3616

Alternate gene names: 21244341

Gene position: 4290693-4290019 (Counterclockwise)

Preceding gene: 21244344

Following gene: 21244338

Centisome position: 82.9

GC content: 69.04

Gene sequence:

>675_bases
ATGCAGCATCCAGCTTTTTATGTCACCGGCACCGATACCGGCATCGGCAAGACCATGGGCAGCACCGCGCTGTTGCACGC
GTTGCGCGCACGCGGCCATCGGGCCGTCGGCATGAAGCCGGTGGCCAGCGGCTGCGAACACACGCCCCACGGCTGGCGCA
ACGAAGACGCGCTCGCGCTGCAGGCGGCCAGCGATCCTCAGCCGGATTACGCCGTGCTCAATCCCTACGCCTTGCCGGCG
CCGCTGGCACCGGAGCTGGCAGCAGCTGAGGTCGGCGTGACGCTGGCGCTGGAGCCGATCGTGCAGGCCTTCGCACAATT
GCGCACGCAGGCCGAGGTGGTGGTGGTGGAAGGCGTCGGTGGCTGGGCCGCGCCGCTGAGTGCGACGCTGGATCAAGCCG
ATCTGGTGTGCGCCTTGCAGCTGCCGGTGGTGCTGGTGGTCGGCGTGCGGCTGGGCTGCATCAACCACGCGCGCCTCACT
GCTGCTGCCATTGCTGCCGATGGGCTGCAGTGCATCGGGTGGATCGCCAATGAAGTGGATCCGCAGATGGAGCGCATCGA
AGAGAACATCGGCATGCTGCGCCAGCGCCTGGCGATGCCGTACTGGGGCCGGATCCCGTGGCGCCCGGGCGCCGACGCTG
CCACCCAGGCGCACGGGCTGCAGTTGCCGCGCTAG

Upstream 100 bases:

>100_bases
GCAGGCAAACGAGAAACAACCGGCCTCAGCCATGGATCCGGTGTGCACGTTATGCTGCGCGCCCTCACGGCAATGGCGGC
GCGGCCGCAGGATCAGGCGT

Downstream 100 bases:

>100_bases
CTGGCACGGCGCGCTTGCGCAGGCAATAAAAAAGCCCGACAGAGGGAGGGATCTGTCGGGCCGGATTGCACGGGGGGAGG
GACCCATGCAAAGACGCTGA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MQHPAFYVTGTDTGIGKTMGSTALLHALRARGHRAVGMKPVASGCEHTPHGWRNEDALALQAASDPQPDYAVLNPYALPA
PLAPELAAAEVGVTLALEPIVQAFAQLRTQAEVVVVEGVGGWAAPLSATLDQADLVCALQLPVVLVVGVRLGCINHARLT
AAAIAADGLQCIGWIANEVDPQMERIEENIGMLRQRLAMPYWGRIPWRPGADAATQAHGLQLPR

Sequences:

>Translated_224_residues
MQHPAFYVTGTDTGIGKTMGSTALLHALRARGHRAVGMKPVASGCEHTPHGWRNEDALALQAASDPQPDYAVLNPYALPA
PLAPELAAAEVGVTLALEPIVQAFAQLRTQAEVVVVEGVGGWAAPLSATLDQADLVCALQLPVVLVVGVRLGCINHARLT
AAAIAADGLQCIGWIANEVDPQMERIEENIGMLRQRLAMPYWGRIPWRPGADAATQAHGLQLPR
>Mature_224_residues
MQHPAFYVTGTDTGIGKTMGSTALLHALRARGHRAVGMKPVASGCEHTPHGWRNEDALALQAASDPQPDYAVLNPYALPA
PLAPELAAAEVGVTLALEPIVQAFAQLRTQAEVVVVEGVGGWAAPLSATLDQADLVCALQLPVVLVVGVRLGCINHARLT
AAAIAADGLQCIGWIANEVDPQMERIEENIGMLRQRLAMPYWGRIPWRPGADAATQAHGLQLPR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=202, Percent_Identity=50, Blast_Score=190, Evalue=6e-50,
Organism=Escherichia coli, GI87081944, Length=207, Percent_Identity=42.9951690821256, Blast_Score=163, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_XANAC (Q8PGK0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643923.1
- ProteinModelPortal:   Q8PGK0
- SMR:   Q8PGK0
- GeneID:   1157687
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3616
- NMPDR:   fig|190486.1.peg.3567
- HOGENOM:   HBG650065
- OMA:   PQMERIE
- ProtClustDB:   PRK00090
- BioCyc:   XAXO190486:XAC3616-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 23720; Mature: 23720

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHPAFYVTGTDTGIGKTMGSTALLHALRARGHRAVGMKPVASGCEHTPHGWRNEDALAL
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEEE
QAASDPQPDYAVLNPYALPAPLAPELAAAEVGVTLALEPIVQAFAQLRTQAEVVVVEGVG
EECCCCCCCEEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEECCC
GWAAPLSATLDQADLVCALQLPVVLVVGVRLGCINHARLTAAAIAADGLQCIGWIANEVD
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PQMERIEENIGMLRQRLAMPYWGRIPWRPGADAATQAHGLQLPR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MQHPAFYVTGTDTGIGKTMGSTALLHALRARGHRAVGMKPVASGCEHTPHGWRNEDALAL
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCEEE
QAASDPQPDYAVLNPYALPAPLAPELAAAEVGVTLALEPIVQAFAQLRTQAEVVVVEGVG
EECCCCCCCEEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEECCC
GWAAPLSATLDQADLVCALQLPVVLVVGVRLGCINHARLTAAAIAADGLQCIGWIANEVD
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PQMERIEENIGMLRQRLAMPYWGRIPWRPGADAATQAHGLQLPR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217