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The map label for this gene is btuB [H]

Identifier: 21244173

GI number: 21244173

Start: 4064850

End: 4067648

Strand: Direct

Name: btuB [H]

Synonym: XAC3448

Alternate gene names: 21244173

Gene position: 4064850-4067648 (Clockwise)

Preceding gene: 21244168

Following gene: 21244176

Centisome position: 78.54

GC content: 61.38

Gene sequence:

>2799_bases
ATGACTATGACAACGCTCCACCCGACGATGCGTCGGGGTTCGCTCGCGATCGCCATCAGCCTGTGCGTCAGCACGAGCGC
CATCGCCCAGGAACAGACCGAGAAGAATCCGACCAACCTGGACAAGATCGAAGTCACCGGTTCGCGCATCCGCTCGGTCG
ATGTTGAAAATTCGCAACCGGTGCTGGTACTCACGCGCCAGGACATCGAAAAGCAGGGCCTGACCTCGGTTGCCGATGTC
CTCAAGCGCATCCCCTCCAACGGTGCGACGATGAACACCACCGTCAACAACGGCGGCGACGGCTCGGCCACGGTGAGCCT
GCGCAACCTGGGCGCCTCACGCACCCTGGTGCTGGTCAATGGCCGGCGGTGGGTCTCGGGCCTGTCCGGCTCGGTGGACC
TGAACACCATTCCCTCGGCCATCGTGGAGCGCGTGGAAGTCCTCAAGGATGGCGCCTCGTCGATCTATGGCTCCGATGCC
ATCGCGGGCGTGGTCAACATCATTACCCGCACCAATTTCGATGGGGCCGAAGTCAACGCCTACGTTGGCCAGTACGGCCA
GGGCGACGGTCAGCGCACCTCCTTCGACGCCACCGTGGGCGCCACCAGCGAGCGCGGCAATCTGGTCATCGGCTTGTCCA
GCGTCAAGGAAGATGCGGTGATGGCGGGCGACCGGGAGATCTCGGCCGAGCCAGTGTACGGCGCGGGCTCGTCGGCCTAT
AGCAGCTACTCGGCAAGCGGCCGCCTCCGTGCAAATGGCACCAGCGGCGCCTGGACGGTGTTGCCCGACAGCGCTACCGC
GAGCGGCGACAGCAGGTATCCGCGCTACGCCCTGGACCAGTACACCGCCTTCAACACATCCGAATATGGCTACAACTACG
CCAAGGACAATTACCTGGTCACGCCACAGAAGCGCGACGCGTTGTACGTGCAGGGCAATTACGCACTCACCGACAACGTG
CGCTTCAAGTTCGATGGACTGTATAACCGCCGCCAGTCGTCGCAGCAACTGGCCGGCTTTCCGCTGGCTTCCGTCAACAC
CGGCATCCTGTTGAGCGGCGACAGCTACTACAACCCGTACAACGCCGCCTACGGCGGCGACGGGCGCGATGTGGGCTGGT
CGCACCGCCTGACCGAATTCGCACGCACCTACCAGCAGGACGTCAAGACCTCGCACGTGTACACCGGGCTGGAAGGCGAC
TTCCAGTTCGCCGACCGGCAGTTCAGTTGGGATGTGGGCTTTGCGCACAACCAGACCGACCAGACCGAAACCCAGTATGG
CGATGTCAATCTGCTCAACCTGAGCAACGCGCTCGGTGCATCCGGCATCGTCAATGGCACCCTGGCGTGCCTGGACAGCG
GCGGCGCCGTCATTGCCGGCTGCGTACCGTTCAACCCCTTGTCGCCGGCCGGTGGCGTGACCCAGGAAATGCTGGACTAC
ATCCTGTTCACTGCGCACAACACATATCAATACAAAAACAGCAGCTTCACCGCCAATCTCACCGGCGATCTGTTCGAGCT
GCCCGCCGGCTGGATGAGTTTTGCTGCCGGTGTCGAACACCGCGAGGAAAGCGGATTCTCCTCGCCGGACGCCTTGATCT
CGGCCGGCTATACCTCGGGCAATTCGTTCACGCCCACCTCTGGCTCGTACACGCTCAACGACTACTACACCGAAGTGGCC
ATCCCACTGCTGAAGGATGTGCCCGGTGCGCAGTTGCTGGAATTGAGCGCAGCAGTGCGTTATTCGGACTACAACACCTT
CGGCAGCACCACCAATAGCAAGTTCGGCTTCAAGTGGAAGCCGGTCGCCGACCTATTGATTCGCGGCAATTACGCGACCG
GTTTCCGCGCGCCATCGATCGAAGATCTGTATCTGGGCGCATCGGACAGCTTCGATACCTATTCCGATCCCTGCTCGACC
AATAGCACCTCCTACACCAGCGCGATCGCGGCCACATGCGCAGCCCGCGGCGTGCCTGCCAATTACGTTGCCGAATACAA
CAGCGCCAGCGGCAACTCCGGCCAGACCATCTATCCGTTCACCTACACCAGCAACGCCAATCTGCAACCGGAAAAATCCA
AGAACACCACGCTTGGTTTTGTCTACAGCCCGGCCTCGGTGCAAGGCCTGGACGTGTCGCTGGACTGGTGGAAGATCGAA
ATCACCGACGCCATCACCGAATTCAGCGCCAATCAGATCCTGTCGCAGTGTTACCAGTTCAACGTCTCGGCGTTCTGCGA
TCTGGTGACCCGCGATGCGACGGGCGCCATCACCGGCCTGGTGATCCAGCCGGTGAATGTCGGCGAGCAACGCATGCAAG
GCTACGATTTCACTGCGCGTTACAAACTGCCGCAGACCGCAATCGGCCAGTTCATGCTCACGCTGGATTCGACCTACGTG
GCGGTCAACGAGCAGAAGCAGGACGACAATTCCGCGTGGGAAAGCTACAACGGCATCTACCACACCAATAACCCGAACTG
GCGCACCCGCGGCACGCTGACCCTGGACTGGAGCTACGGCAATCTGGCCGCCAACTGGGCCGTGCGTTACTACTCGGGCA
TGAAGGACTACGATTCGGAACTGGACGACAACGGAAACTATCGCCACGTTGCGGCGACCGCGTTCAACGACATGCAGGTG
TCTTACCAACTTCCATGGAATGCGACCGTGCGGTTGGGTCTGAACAACGTCTTCGACCGCGACCCGCCGGTCGCGCTCAG
CGCCTCGGCCAACTCGTTCGATTCGGCCTATGCCATCCCGGGACGCTACAGCTACCTGCAATACACGCAGCGCTTCTGA

Upstream 100 bases:

>100_bases
CCGCTAAGCCTGCGTGGCGTGCCGAAGACACCGCACGCATGCAGGTGTCATTCACGGGGATCGCGCTGTCGCTCCTCCTC
TACCGGGAACCGCCGTACAT

Downstream 100 bases:

>100_bases
GCGCGCTGTCCTGATACACGTGTTGTCACGACGGCCTCCTTCGGGAGGCCGTTGTCTGTGTGGCACGGCTAACACGCAAT
CGGGCGACTGGCACACGGCA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 932; Mature: 931

Protein sequence:

>932_residues
MTMTTLHPTMRRGSLAIAISLCVSTSAIAQEQTEKNPTNLDKIEVTGSRIRSVDVENSQPVLVLTRQDIEKQGLTSVADV
LKRIPSNGATMNTTVNNGGDGSATVSLRNLGASRTLVLVNGRRWVSGLSGSVDLNTIPSAIVERVEVLKDGASSIYGSDA
IAGVVNIITRTNFDGAEVNAYVGQYGQGDGQRTSFDATVGATSERGNLVIGLSSVKEDAVMAGDREISAEPVYGAGSSAY
SSYSASGRLRANGTSGAWTVLPDSATASGDSRYPRYALDQYTAFNTSEYGYNYAKDNYLVTPQKRDALYVQGNYALTDNV
RFKFDGLYNRRQSSQQLAGFPLASVNTGILLSGDSYYNPYNAAYGGDGRDVGWSHRLTEFARTYQQDVKTSHVYTGLEGD
FQFADRQFSWDVGFAHNQTDQTETQYGDVNLLNLSNALGASGIVNGTLACLDSGGAVIAGCVPFNPLSPAGGVTQEMLDY
ILFTAHNTYQYKNSSFTANLTGDLFELPAGWMSFAAGVEHREESGFSSPDALISAGYTSGNSFTPTSGSYTLNDYYTEVA
IPLLKDVPGAQLLELSAAVRYSDYNTFGSTTNSKFGFKWKPVADLLIRGNYATGFRAPSIEDLYLGASDSFDTYSDPCST
NSTSYTSAIAATCAARGVPANYVAEYNSASGNSGQTIYPFTYTSNANLQPEKSKNTTLGFVYSPASVQGLDVSLDWWKIE
ITDAITEFSANQILSQCYQFNVSAFCDLVTRDATGAITGLVIQPVNVGEQRMQGYDFTARYKLPQTAIGQFMLTLDSTYV
AVNEQKQDDNSAWESYNGIYHTNNPNWRTRGTLTLDWSYGNLAANWAVRYYSGMKDYDSELDDNGNYRHVAATAFNDMQV
SYQLPWNATVRLGLNNVFDRDPPVALSASANSFDSAYAIPGRYSYLQYTQRF

Sequences:

>Translated_932_residues
MTMTTLHPTMRRGSLAIAISLCVSTSAIAQEQTEKNPTNLDKIEVTGSRIRSVDVENSQPVLVLTRQDIEKQGLTSVADV
LKRIPSNGATMNTTVNNGGDGSATVSLRNLGASRTLVLVNGRRWVSGLSGSVDLNTIPSAIVERVEVLKDGASSIYGSDA
IAGVVNIITRTNFDGAEVNAYVGQYGQGDGQRTSFDATVGATSERGNLVIGLSSVKEDAVMAGDREISAEPVYGAGSSAY
SSYSASGRLRANGTSGAWTVLPDSATASGDSRYPRYALDQYTAFNTSEYGYNYAKDNYLVTPQKRDALYVQGNYALTDNV
RFKFDGLYNRRQSSQQLAGFPLASVNTGILLSGDSYYNPYNAAYGGDGRDVGWSHRLTEFARTYQQDVKTSHVYTGLEGD
FQFADRQFSWDVGFAHNQTDQTETQYGDVNLLNLSNALGASGIVNGTLACLDSGGAVIAGCVPFNPLSPAGGVTQEMLDY
ILFTAHNTYQYKNSSFTANLTGDLFELPAGWMSFAAGVEHREESGFSSPDALISAGYTSGNSFTPTSGSYTLNDYYTEVA
IPLLKDVPGAQLLELSAAVRYSDYNTFGSTTNSKFGFKWKPVADLLIRGNYATGFRAPSIEDLYLGASDSFDTYSDPCST
NSTSYTSAIAATCAARGVPANYVAEYNSASGNSGQTIYPFTYTSNANLQPEKSKNTTLGFVYSPASVQGLDVSLDWWKIE
ITDAITEFSANQILSQCYQFNVSAFCDLVTRDATGAITGLVIQPVNVGEQRMQGYDFTARYKLPQTAIGQFMLTLDSTYV
AVNEQKQDDNSAWESYNGIYHTNNPNWRTRGTLTLDWSYGNLAANWAVRYYSGMKDYDSELDDNGNYRHVAATAFNDMQV
SYQLPWNATVRLGLNNVFDRDPPVALSASANSFDSAYAIPGRYSYLQYTQRF
>Mature_931_residues
TMTTLHPTMRRGSLAIAISLCVSTSAIAQEQTEKNPTNLDKIEVTGSRIRSVDVENSQPVLVLTRQDIEKQGLTSVADVL
KRIPSNGATMNTTVNNGGDGSATVSLRNLGASRTLVLVNGRRWVSGLSGSVDLNTIPSAIVERVEVLKDGASSIYGSDAI
AGVVNIITRTNFDGAEVNAYVGQYGQGDGQRTSFDATVGATSERGNLVIGLSSVKEDAVMAGDREISAEPVYGAGSSAYS
SYSASGRLRANGTSGAWTVLPDSATASGDSRYPRYALDQYTAFNTSEYGYNYAKDNYLVTPQKRDALYVQGNYALTDNVR
FKFDGLYNRRQSSQQLAGFPLASVNTGILLSGDSYYNPYNAAYGGDGRDVGWSHRLTEFARTYQQDVKTSHVYTGLEGDF
QFADRQFSWDVGFAHNQTDQTETQYGDVNLLNLSNALGASGIVNGTLACLDSGGAVIAGCVPFNPLSPAGGVTQEMLDYI
LFTAHNTYQYKNSSFTANLTGDLFELPAGWMSFAAGVEHREESGFSSPDALISAGYTSGNSFTPTSGSYTLNDYYTEVAI
PLLKDVPGAQLLELSAAVRYSDYNTFGSTTNSKFGFKWKPVADLLIRGNYATGFRAPSIEDLYLGASDSFDTYSDPCSTN
STSYTSAIAATCAARGVPANYVAEYNSASGNSGQTIYPFTYTSNANLQPEKSKNTTLGFVYSPASVQGLDVSLDWWKIEI
TDAITEFSANQILSQCYQFNVSAFCDLVTRDATGAITGLVIQPVNVGEQRMQGYDFTARYKLPQTAIGQFMLTLDSTYVA
VNEQKQDDNSAWESYNGIYHTNNPNWRTRGTLTLDWSYGNLAANWAVRYYSGMKDYDSELDDNGNYRHVAATAFNDMQVS
YQLPWNATVRLGLNNVFDRDPPVALSASANSFDSAYAIPGRYSYLQYTQRF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=172, Percent_Identity=39.5348837209302, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI1788478, Length=209, Percent_Identity=31.1004784688995, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1786798, Length=170, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 101125; Mature: 100994

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTTLHPTMRRGSLAIAISLCVSTSAIAQEQTEKNPTNLDKIEVTGSRIRSVDVENSQP
CCEEECCHHHHCCCEEEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCC
VLVLTRQDIEKQGLTSVADVLKRIPSNGATMNTTVNNGGDGSATVSLRNLGASRTLVLVN
EEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEE
GRRWVSGLSGSVDLNTIPSAIVERVEVLKDGASSIYGSDAIAGVVNIITRTNFDGAEVNA
CCHHCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEE
YVGQYGQGDGQRTSFDATVGATSERGNLVIGLSSVKEDAVMAGDREISAEPVYGAGSSAY
ECCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCCCCHHC
SSYSASGRLRANGTSGAWTVLPDSATASGDSRYPRYALDQYTAFNTSEYGYNYAKDNYLV
CCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHEECCCCCCCCCCCCCCEEE
TPQKRDALYVQGNYALTDNVRFKFDGLYNRRQSSQQLAGFPLASVNTGILLSGDSYYNPY
CCCCCCEEEEECCEEEECCEEEEECCHHHHCCCCHHHCCCCCCCCCCCEEECCCCCCCCC
NAAYGGDGRDVGWSHRLTEFARTYQQDVKTSHVYTGLEGDFQFADRQFSWDVGFAHNQTD
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCEEEEEECCCCCCCC
QTETQYGDVNLLNLSNALGASGIVNGTLACLDSGGAVIAGCVPFNPLSPAGGVTQEMLDY
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHH
ILFTAHNTYQYKNSSFTANLTGDLFELPAGWMSFAAGVEHREESGFSSPDALISAGYTSG
HEEECCCCEEECCCCEEEECCCCHHHCCHHHHHHHHCCCHHHHCCCCCCCHHEEECCCCC
NSFTPTSGSYTLNDYYTEVAIPLLKDVPGAQLLELSAAVRYSDYNTFGSTTNSKFGFKWK
CCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCEEEC
PVADLLIRGNYATGFRAPSIEDLYLGASDSFDTYSDPCSTNSTSYTSAIAATCAARGVPA
CHHHEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
NYVAEYNSASGNSGQTIYPFTYTSNANLQPEKSKNTTLGFVYSPASVQGLDVSLDWWKIE
HHHEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEEE
ITDAITEFSANQILSQCYQFNVSAFCDLVTRDATGAITGLVIQPVNVGEQRMQGYDFTAR
EEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHCCCCEEEE
YKLPQTAIGQFMLTLDSTYVAVNEQKQDDNSAWESYNGIYHTNNPNWRTRGTLTLDWSYG
ECCCHHHHHHEEEEECCEEEEEECCCCCCCHHHHHCCCEEECCCCCEEEEEEEEEEECCC
NLAANWAVRYYSGMKDYDSELDDNGNYRHVAATAFNDMQVSYQLPWNATVRLGLNNVFDR
CEEEEEEEEECCCCCHHHHCCCCCCCEEEEEEEEECCEEEEEECCCCCEEEECCCCCCCC
DPPVALSASANSFDSAYAIPGRYSYLQYTQRF
CCCEEEECCCCCCCCEEECCCCCHHHHHHHCC
>Mature Secondary Structure 
TMTTLHPTMRRGSLAIAISLCVSTSAIAQEQTEKNPTNLDKIEVTGSRIRSVDVENSQP
CEEECCHHHHCCCEEEEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCC
VLVLTRQDIEKQGLTSVADVLKRIPSNGATMNTTVNNGGDGSATVSLRNLGASRTLVLVN
EEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEE
GRRWVSGLSGSVDLNTIPSAIVERVEVLKDGASSIYGSDAIAGVVNIITRTNFDGAEVNA
CCHHCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEE
YVGQYGQGDGQRTSFDATVGATSERGNLVIGLSSVKEDAVMAGDREISAEPVYGAGSSAY
ECCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCCCCHHC
SSYSASGRLRANGTSGAWTVLPDSATASGDSRYPRYALDQYTAFNTSEYGYNYAKDNYLV
CCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHEECCCCCCCCCCCCCCEEE
TPQKRDALYVQGNYALTDNVRFKFDGLYNRRQSSQQLAGFPLASVNTGILLSGDSYYNPY
CCCCCCEEEEECCEEEECCEEEEECCHHHHCCCCHHHCCCCCCCCCCCEEECCCCCCCCC
NAAYGGDGRDVGWSHRLTEFARTYQQDVKTSHVYTGLEGDFQFADRQFSWDVGFAHNQTD
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCEEEEEECCCCCCCC
QTETQYGDVNLLNLSNALGASGIVNGTLACLDSGGAVIAGCVPFNPLSPAGGVTQEMLDY
CCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHH
ILFTAHNTYQYKNSSFTANLTGDLFELPAGWMSFAAGVEHREESGFSSPDALISAGYTSG
HEEECCCCEEECCCCEEEECCCCHHHCCHHHHHHHHCCCHHHHCCCCCCCHHEEECCCCC
NSFTPTSGSYTLNDYYTEVAIPLLKDVPGAQLLELSAAVRYSDYNTFGSTTNSKFGFKWK
CCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCEEEC
PVADLLIRGNYATGFRAPSIEDLYLGASDSFDTYSDPCSTNSTSYTSAIAATCAARGVPA
CHHHEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
NYVAEYNSASGNSGQTIYPFTYTSNANLQPEKSKNTTLGFVYSPASVQGLDVSLDWWKIE
HHHEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEEEEE
ITDAITEFSANQILSQCYQFNVSAFCDLVTRDATGAITGLVIQPVNVGEQRMQGYDFTAR
EEHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHCCCCEEEE
YKLPQTAIGQFMLTLDSTYVAVNEQKQDDNSAWESYNGIYHTNNPNWRTRGTLTLDWSYG
ECCCHHHHHHEEEEECCEEEEEECCCCCCCHHHHHCCCEEECCCCCEEEEEEEEEEECCC
NLAANWAVRYYSGMKDYDSELDDNGNYRHVAATAFNDMQVSYQLPWNATVRLGLNNVFDR
CEEEEEEEEECCCCCHHHHCCCCCCCEEEEEEEEECCEEEEEECCCCCEEEECCCCCCCC
DPPVALSASANSFDSAYAIPGRYSYLQYTQRF
CCCEEEECCCCCCCCEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA