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The map label for this gene is thiG

Identifier: 21244027

GI number: 21244027

Start: 3880131

End: 3880925

Strand: Direct

Name: thiG

Synonym: XAC3302

Alternate gene names: 21244027

Gene position: 3880131-3880925 (Clockwise)

Preceding gene: 21244026

Following gene: 21244028

Centisome position: 74.97

GC content: 65.79

Gene sequence:

>795_bases
ATGACGAATCCCGCCCCCTCTGATGCGCTGGTCATCGCCGGCAAACACTACCGTTCGCGCCTGCTCACCGGCACCGGCAA
GTTCAAGGATCTGGACGAAACCCGCCTGGCCACCGAAGCCGCCGCGGCCGAAATCGTCACTGTCGCCATTCGCCGGGTGA
ATATTGGCCAGGACCCCAACGCACCCAGCCTGCTGGATGTGCTGCCGCCGGAGCGCTACACGCTGCTGCCCAACACCGCC
GGCTGCTACACCGCCGAGGACGCGGTGCGCACGTGCCGGCTGGCGCGCGAACTGCTGGACGGCCACAACCTCACCAAGCT
GGAAGTGCTCGGCGACGAGAAGACGCTGTACCCGGATGTGGTGCAGACGCTCAAGGCCGCCGAACAACTGGTCGCCGATG
GTTTCGAGGTGATGGTCTACACCTCCGACGACCCGATCCTGGCCAAGCGCCTGGAAGACATCGGCTGCGTGGCGGTGATG
CCGCTAGCCGCGCCGATCGGCTCGGGGCTGGGCATCCAGAACAAGTACAACCTGCTGGAAATCATCGAGAACGCCAAGGT
GCCGATCATCGTGGATGCCGGGGTGGGCACCGCCTCGGATGCGGCGATTGCCATGGAGCTGGGCTGCGATGGCGTGCTGA
TGAATACCGCCATTGCCGGTGCGCGCGACCCGATCCTGATGGCCAGCGCGATGCGCAAGGCGATCGAGGCCGGACGCGAG
GCGTTCCTGGCCGGGCGGATTCCACGCAAGCGCTATGCATCGGCGTCGAGCCCGGTGGATGGTGTGATTGGATGA

Upstream 100 bases:

>100_bases
TTACGCGAGGGCGATGTGGTGGAAATCGTGCACGCGCTGGGCGGCGGCTGATGCGTCGCGCAGGCCGGCGCATCAGCGCC
CGATCGGTGATAATTGCCGC

Downstream 100 bases:

>100_bases
TGGGGCGGTCTGCGTTGACATTGCGGCCGAGGCGCAATGATTGGCAGTGCGGCGTGCGGAATATGACCGCTGCCAGATCG
CGCGTTGCTACGAGCGCCCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTNPAPSDALVIAGKHYRSRLLTGTGKFKDLDETRLATEAAAAEIVTVAIRRVNIGQDPNAPSLLDVLPPERYTLLPNTA
GCYTAEDAVRTCRLARELLDGHNLTKLEVLGDEKTLYPDVVQTLKAAEQLVADGFEVMVYTSDDPILAKRLEDIGCVAVM
PLAAPIGSGLGIQNKYNLLEIIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
AFLAGRIPRKRYASASSPVDGVIG

Sequences:

>Translated_264_residues
MTNPAPSDALVIAGKHYRSRLLTGTGKFKDLDETRLATEAAAAEIVTVAIRRVNIGQDPNAPSLLDVLPPERYTLLPNTA
GCYTAEDAVRTCRLARELLDGHNLTKLEVLGDEKTLYPDVVQTLKAAEQLVADGFEVMVYTSDDPILAKRLEDIGCVAVM
PLAAPIGSGLGIQNKYNLLEIIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
AFLAGRIPRKRYASASSPVDGVIG
>Mature_263_residues
TNPAPSDALVIAGKHYRSRLLTGTGKFKDLDETRLATEAAAAEIVTVAIRRVNIGQDPNAPSLLDVLPPERYTLLPNTAG
CYTAEDAVRTCRLARELLDGHNLTKLEVLGDEKTLYPDVVQTLKAAEQLVADGFEVMVYTSDDPILAKRLEDIGCVAVMP
LAAPIGSGLGIQNKYNLLEIIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGREA
FLAGRIPRKRYASASSPVDGVIG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=254, Percent_Identity=48.8188976377953, Blast_Score=218, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_XANAC (Q8PHF5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643609.1
- ProteinModelPortal:   Q8PHF5
- SMR:   Q8PHF5
- GeneID:   1157373
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3302
- NMPDR:   fig|190486.1.peg.3253
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   XAXO190486:XAC3302-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27939; Mature: 27807

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 106-106 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPAPSDALVIAGKHYRSRLLTGTGKFKDLDETRLATEAAAAEIVTVAIRRVNIGQDPN
CCCCCCCCEEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APSLLDVLPPERYTLLPNTAGCYTAEDAVRTCRLARELLDGHNLTKLEVLGDEKTLYPDV
CCCCEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
VQTLKAAEQLVADGFEVMVYTSDDPILAKRLEDIGCVAVMPLAAPIGSGLGIQNKYNLLE
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCEEEHHHHHHCCCCCCCCCCHHHHH
IIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
HHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHH
AFLAGRIPRKRYASASSPVDGVIG
HHHHCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TNPAPSDALVIAGKHYRSRLLTGTGKFKDLDETRLATEAAAAEIVTVAIRRVNIGQDPN
CCCCCCCEEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APSLLDVLPPERYTLLPNTAGCYTAEDAVRTCRLARELLDGHNLTKLEVLGDEKTLYPDV
CCCCEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
VQTLKAAEQLVADGFEVMVYTSDDPILAKRLEDIGCVAVMPLAAPIGSGLGIQNKYNLLE
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHCCCEEEHHHHHHCCCCCCCCCCHHHHH
IIENAKVPIIVDAGVGTASDAAIAMELGCDGVLMNTAIAGARDPILMASAMRKAIEAGRE
HHHCCCCCEEEECCCCCCCCCEEEEEECCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHH
AFLAGRIPRKRYASASSPVDGVIG
HHHHCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217